Exosomes and microvesicles最新文献_第2页

Dynamics of post-stroke hand paresis kinematic pattern during rehabilitation 康复期间中风后手部麻痹运动模式的动力学研究

Exosomes and microvesicles Pub Date : 2019-08-31 DOI: 10.24075/brsmu.2019.056

A. Khizhnikova, A. Klochkov, A. Kotov–Smolenskiy, N. Suponeva, M. Piradov

{"title":"Dynamics of post-stroke hand paresis kinematic pattern during rehabilitation","authors":"A. Khizhnikova, A. Klochkov, A. Kotov–Smolenskiy, N. Suponeva, M. Piradov","doi":"10.24075/brsmu.2019.056","DOIUrl":"https://doi.org/10.24075/brsmu.2019.056","url":null,"abstract":"According to the literature data, only 5–20% of post-stroke patients are able to restore the hand motor function completely. Correct goal setting and individual approach to the patient's functional recovery are important. Our study aimed to develop an algorithm of impaired hand motor functioning assessment for post-stroke patients and to determine the principles of the rehabilitation tactics choosing based on the biomechanical analysis. Twenty five patients with hemispheric stroke and 10 healthy volunteers participated in the study. Formal clinical observation scales (Fugl-Meyer Assessment, Ashworth Scale, ARAT) and video motion analysis were used for evaluation of the hand motor function. Patients were divided into 2 groups according to the hand paresis severity (mild/moderate and pronounced/severe). Rehabilitation was carried out in both groups, including mechanotherapy, massage and physical therapy. It was revealed that in the 1st group of patients the motor function recovery in the paretic hand was due to movement performance recovery: biomechanical parameters restoration directly correlated with a decrease in the paresis degree according to the Fugl-Meyer Assessment Scale (r = 0.94; p = 0.01). In the 2nd group of patients, the motor function recovery in the paretic hand was due to motor deficit compensation: according to biomechanical analysis, the pathological motor synergies inversely correlated with a decrease in the paresis degree (r = –0.9; p = 0.03). As a result of the study, an algorithm for selecting the patient management tactics based on the baseline clinical indicators was developed.","PeriodicalId":90498,"journal":{"name":"Exosomes and microvesicles","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89735827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A medical career: barriers to professional identity 医疗事业:职业认同的障碍

Exosomes and microvesicles Pub Date : 2019-08-26 DOI: 10.24075/brsmu.2019.055

E. Mettini, B. Yasko, B. Kazarin, M. Ostroushko

引用次数: 0

Associations between blood and cerebrospinal fluid flow impairments assessed with phase-contrast MRI and brain damage in patients with age-related cerebral small vessel disease 与年龄相关的脑小血管疾病患者的相衬MRI评估血液和脑脊液流动障碍与脑损伤之间的关系

Exosomes and microvesicles Pub Date : 2019-08-25 DOI: 10.24075/brsmu.2019.054

E. Kremneva, B. M. Akhmetzyanov, L. Dobrynina, M. Krotenkova

{"title":"Associations between blood and cerebrospinal fluid flow impairments assessed with phase-contrast MRI and brain damage in patients with age-related cerebral small vessel disease","authors":"E. Kremneva, B. M. Akhmetzyanov, L. Dobrynina, M. Krotenkova","doi":"10.24075/brsmu.2019.054","DOIUrl":"https://doi.org/10.24075/brsmu.2019.054","url":null,"abstract":"Hemodynamic parameters of blood and cerebrospinal fluid (CSF) flow can be measured in vivo using phase-contrast MRI (PC-MRI). This opens new horizons for studying the mechanisms implicated in the development and progression of age-related cerebral small vessel disease (SVD). In this paper, we analyze associations between cerebral arterial, venous and CSF flow impairments and SVD features visible on MRI. The study was carried out in 96 patients with SVD (aged 60.91 ± 6.57 years) and 23 healthy volunteers (59.13 ± 6.56 years). The protocol of the MRI examination included routine MRI sequences (T2, FLAIR, T1, SWI, and DWI) applied to assess the severity of brain damage according to STRIVE advisory standards and PC-MRI used to quantify blood flow in the major arteries and veins of the neck, the straight and upper sagittal sinuses, and CSF flow at the aqueduct level. We analyzed the associations between linear and volumetric parameters of blood/CSF flow and the degree of brain matter damage using the Fazekas scale. We observed a reduction in tABF, stVBF, sssVBF, aqLF, Saq, and ICC values and a rise in Pi associated with WMH progression, as well as a gradual decline in tABF and an increase in Pi, Saq and ICC associated with a growing number of lacunes (р < 0.05). Patients with early (< 5) MB had lower sssVBF and stVBF rates in comparison with patients without MB; aqLF, Saq, and ICC values were elevated in patients with 5 to 10 MB, as compared to patients without MB or early (< 5) MB. The established associations between MRI findings in patients with SVD and blood/CSF flow impairments suggest the important role of mechanisms implicated in the disruption of Monro–Kellie intracranial homeostasis in promoting SVD.","PeriodicalId":90498,"journal":{"name":"Exosomes and microvesicles","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83037150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The feasibility of using computer-based models for reducing the risks of complications associated with temporary dentures 使用计算机模型降低临时假牙并发症风险的可行性

Exosomes and microvesicles Pub Date : 2019-08-16 DOI: 10.24075/brsmu.2019.050

N. V. Bagryantseva, S. Gazhva, A. A. Baranov, L. Shubin, V. A. Bagryantsev, O. Bagryantseva

引用次数: 0

Application of culture-based, mass spectrometry and molecular methods to the study of gut microbiota in children 基于培养、质谱和分子方法在儿童肠道微生物群研究中的应用

Exosomes and microvesicles Pub Date : 2019-08-09 DOI: 10.24075/brsmu.2019.048

Boris A. Efimov, A. V. Chaplin, S. R. Sokolova, Z. A. Chernaia, A. Pikina, A. M. Savilova, L. Kafarskaya

{"title":"Application of culture-based, mass spectrometry and molecular methods to the study of gut microbiota in children","authors":"Boris A. Efimov, A. V. Chaplin, S. R. Sokolova, Z. A. Chernaia, A. Pikina, A. M. Savilova, L. Kafarskaya","doi":"10.24075/brsmu.2019.048","DOIUrl":"https://doi.org/10.24075/brsmu.2019.048","url":null,"abstract":"In recent decades, nucleic acid sequencing technologies used for metagenomic analysis have become the main methods for assessing the composition of microbiota. At the same time, the use of novel methods of cultivation and identification of microorganisms in microbiological research led to the renaissance of culture-based technologies, because facilitated the discovery and isolation of both new strains of well-known microorganisms as well as uncultivated and unexplored bacterial taxa. The aim of this study was to evaluate the potential of using the culture-based method for the assessment of the qualitative and quantitative composition of the intestinal microbiota in healthy children. Eleven growth media were inoculated with serial dilutions of stool samples in order to analyze the profile of dominant anaerobic bacteria, as well as aerobic bacteria and fungi in 20 healthy children aged 2–4 years. The identification of microorganisms was performed using MALDI TOF MS and 16S rRNA gene fragment sequencing were used. 1,819 isolated and identified strains belong to 7 phyla, 13 classes, 18 orders, 33 families, 77 genera and 149 species in the Bacteria domain. The Bacteroidetes, Firmicutes, Actinobacteria and Proteobacteria phyla were most abundant and frequent. The greatest species diversity (more than 85 species) was found in the Firmicutes phylum. Ten new previously uncharacterized bacterial strains were isolated.","PeriodicalId":90498,"journal":{"name":"Exosomes and microvesicles","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72625026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Synchrotron IR-microspectroscopy-based visualization of molecular and chemical interactions between dental cement, biomimetic composite and native dental tissue 基于同步加速器红外显微光谱的牙水泥、仿生复合材料和原生牙组织之间分子和化学相互作用的可视化研究

Exosomes and microvesicles Pub Date : 2019-08-01 DOI: 10.24075/brsmu.2019.047

D. Goloshchapov, V. Kashkarov, Y. Ippolitov, I. Ippolitov, J. Vongsvivut, P. Seredin

{"title":"Synchrotron IR-microspectroscopy-based visualization of molecular and chemical interactions between dental cement, biomimetic composite and native dental tissue","authors":"D. Goloshchapov, V. Kashkarov, Y. Ippolitov, I. Ippolitov, J. Vongsvivut, P. Seredin","doi":"10.24075/brsmu.2019.047","DOIUrl":"https://doi.org/10.24075/brsmu.2019.047","url":null,"abstract":"The low affinity of composite materials for the hard tissue of human teeth poses a challenge to restorative dentists. This study was undertaken to explore molecular and chemical characteristics of the interface between the dental cement, the buffer layer formed from a next generation biomimetic material that mimics the organic mineral composition of human enamel and dentin, and the intact native hard dental tissue. Seven plane-parallel dental slices were analyzed using synchrotron IR microspectroscopy. The obtained absorption spectra of functional molecular groups were organized into cluster maps. This allowed us to identify the intact tissue, the adhesive agent and the biomimetic layer at their interface and to localize and measure concentrations of functional groups involved in the integration of the biomimetic composite into the hard tissue of the human tooth. The proposed biomimetic material is based on nanocrystal carbonate-substituted calcium hydroxyapatite synthesized from a biogenic calcium source and a complex of basic polar amino acids copying the composition of the human tooth and can form a functional bond with hard dental tissue.","PeriodicalId":90498,"journal":{"name":"Exosomes and microvesicles","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87291213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The impact of electronic devices on the physical growth and development of modern youth and recommendations on their safe use 电子设备对现代青少年身体生长发育的影响及安全使用的建议

Exosomes and microvesicles Pub Date : 2019-07-23 DOI: 10.24075/brsmu.2019.046

O. Milushkina, N. Skoblina, S. Markelova, A. Tatarinchik, E. P. Melikhova, I. I. Libina, M. Popov

{"title":"The impact of electronic devices on the physical growth and development of modern youth and recommendations on their safe use","authors":"O. Milushkina, N. Skoblina, S. Markelova, A. Tatarinchik, E. P. Melikhova, I. I. Libina, M. Popov","doi":"10.24075/brsmu.2019.046","DOIUrl":"https://doi.org/10.24075/brsmu.2019.046","url":null,"abstract":"The impact of excessive exposure to electronic devices (ED) on youth health remains understudied. There is a pressing need to develop recommendations for the safe use of stationary and mobile ED aimed at minimizing health risks. In this work, we assess the effect of ED on the physical growth and development of high-school and university students and provide recommendations for preventing the negative impact of prolonged screen time on health. The study recruited 460 high-school and 598 university students. Standard anthropometric measurements were taken. The psychological and emotional state of the participants was evaluated using the Test Anxiety Inventory by Spielberg (modified by Khanin). To estimate daily and weekly exposure to ED the participants were asked to fill out standardized questionnaires. In high school students, the average screen time was 7 h a day; in university students, 8.5 to 10 h a day. Only 60% of the participants, regardless of their place of residence or the type of educational institution they were attending, were physically healthy. We conclude that prolonged and frequent exposure to ED is one of the factors that can interfere with normal physical growth and development in youth. Regular daily use of stationary ED increases the risk of developing body weight deficit by 24% and gaining excess body weight by 10%. We recommend that students should eliminate computers, laptops and stationary ED from their daily activities for at least one day at the weekend and reduce total screen time to 3 hours a day.","PeriodicalId":90498,"journal":{"name":"Exosomes and microvesicles","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86447732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Molecular-genetic and phenotypic characteristics of desmoid-type fibromatosis 硬纤维瘤病的分子遗传学和表型特征

Exosomes and microvesicles Pub Date : 2019-07-18 DOI: 10.24075/brsmu.2019.045

А. Музаффарова, О. В. Новикова, Ю. Сачков, Ф. М. Кипкеева, Е. К. Гинтер, А. В. Карпухин, OV Novikova, Sachkov IYu, EK Ginter

{"title":"Molecular-genetic and phenotypic characteristics of desmoid-type fibromatosis","authors":"А. Музаффарова, О. В. Новикова, Ю. Сачков, Ф. М. Кипкеева, Е. К. Гинтер, А. В. Карпухин, OV Novikova, Sachkov IYu, EK Ginter","doi":"10.24075/brsmu.2019.045","DOIUrl":"https://doi.org/10.24075/brsmu.2019.045","url":null,"abstract":"Desmoid-type fibromatosis (DF) is a rare mesenchymal tumor occurring in only 2 to 4 people per 1,000,000 population a year. Desmoid tumors are either seen sporadically or in individuals with familial adenomatous polyposis (FAP). The etiology of sporadic DF is uncertain. The aim of this study was to estimate the potential significance of germline mutations in the APC gene in patients with sporadic DF. APC exons were amplified, studied using conformation sensitive gel electrophoresis and then Sanger-sequenced. The obtained data were processed in Statistica 10. Mutations were detected in 6 (12%) of 51 participants with sporadic DF. Those 6 patients shared a typical DF phenotype characterized by early age of onset (5.8 years on average, in contrast to the patients without APC mutations, who developed DF at 19 years of age; p = 0.02), severe clinical course, multifocal localization on the trunk, and poor prognosis. All of the detected APC mutations were localized to the 3'-end of the gene. For the purpose of comparison, we analyzed a sample of 12 patients with FAP-associated DF. Of those patients, 6 carried mutations in the APC gene. In the analyzed sample, the patients with FAP and the mutant APC gene developed DF at older age (35 years) than the patients with sporadic DF (p = 0.004) and their tumors were not multifocal. This means that sporadic and FAP-associated desmoids have different phenotypes in patients with APC mutations. Patients with sporadic tumors have mutations at the 3'-end of the APC gene more often than individuals with FAP-associated DF. To our knowledge, this is the first study to characterize the subtype of sporadic desmoid fibromatosis phenotypically determined by germline mutations in the APC gene.","PeriodicalId":90498,"journal":{"name":"Exosomes and microvesicles","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80029184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular Vesicles in Heart Disease: Excitement for the Future ? 心脏疾病中的细胞外囊泡:令人兴奋的未来?

Exosomes and microvesicles Pub Date : 2014-01-01 DOI: 10.5772/58390

Kirsty M Danielson, Saumya Das

引用次数: 48

Perspectives on the Potential Therapeutic Uses of Vesicles. 对囊泡潜在治疗用途的展望。

Exosomes and microvesicles Pub Date : 2013-01-01 DOI: 10.5772/57393

Giovanni Camussi, Peter J Quesenberry

引用次数: 23