Bone Reports最新文献

{"title":"Bone hierarchical organization through the lens of materials science: Present opportunities and future challenges","authors":"Chiara Micheletti,&nbsp;Furqan A. Shah","doi":"10.1016/j.bonr.2024.101783","DOIUrl":"https://doi.org/10.1016/j.bonr.2024.101783","url":null,"abstract":"<div><p>Multiscale characterization is essential to better understand the hierarchical architecture of bone and an array of analytical methods contributes to exploring the various structural and compositional aspects. Incorporating X-ray tomography, X-ray scattering, vibrational spectroscopy, and atom probe tomography alongside electron microscopy provides a comprehensive approach, offering insights into the diverse levels of organization within bone. X-ray scattering techniques reveal information about collagen-mineral spatial relationships, while X-ray tomography captures 3D structural details, especially at the microscale. Electron microscopy, such as scanning and transmission electron microscopy, extends resolution to the nanoscale, showcasing intricate features such as collagen fibril organization. Additionally, atom probe tomography achieves sub-nanoscale resolution and high chemical sensitivity, enabling detailed examination of bone composition. Despite various technical challenges, a correlative approach allows for a comprehensive understanding of bone material properties. Real-time investigations through in situ and <em>in operando</em> approaches shed light on the dynamic processes in bone. Recently developed techniques such as liquid, in situ transmission electron microscopy provide insights into calcium phosphate formation and collagen mineralization. Mechanical models developed in the effort to link structure, composition, and function currently remain oversimplified but can be improved. In conclusion, correlative analytical platforms provide a holistic perspective of bone extracellular matrix and are essential for unraveling the intricate interplay between structure and composition within bone.</p></div>","PeriodicalId":9043,"journal":{"name":"Bone Reports","volume":"22 ","pages":"Article 101783"},"PeriodicalIF":2.1,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352187224000500/pdfft?md5=0467c214373ede185f74a2dfc951bb42&pid=1-s2.0-S2352187224000500-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141595669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DXA evaluation of bone fragility 2 years after bariatric surgery in patients with obesity","authors":"Marine Fauny ,&nbsp;Marion Halin ,&nbsp;Edem Allado ,&nbsp;Laurent Brunaud ,&nbsp;Claire Nomine-Criqui ,&nbsp;Eliane Albuisson ,&nbsp;Isabelle Chary-Valckenaere ,&nbsp;Didier Quilliot ,&nbsp;Damien Loeuille","doi":"10.1016/j.bonr.2024.101782","DOIUrl":"https://doi.org/10.1016/j.bonr.2024.101782","url":null,"abstract":"<div><h3>Purpose</h3><p>The primary objective was to evaluate bone fragility on dual X-ray absorptiometry (DXA) in patients with obesity before and 2 years after bariatric surgery. The secondary objective was to identify risk factors for the development of a bone mineral density ≤ −2 SD at 2 years.</p></div><div><h3>Methods</h3><p>This descriptive study included patients with obesity who underwent DXA before and 2 years (±6 months) after bariatric surgery. The BMD and the T-score were assessed at the lumbar spine, femoral neck and total hip. Data on body composition on DXA were also collected. The diagnosis of osteoporosis was retained for a T-score ≤ − 2.5 SD at any measured location. Osteopenia, or low bone mass, was defined by −2.5 SD &lt; T-score ≤ −1 SD.</p></div><div><h3>Results</h3><p>Among the 675 included patients, 77.8 % were women, with a mean age of 49.5 years (±11.1). After bariatric surgery, there were significantly more patients with osteoporosis: 3.6 % vs. 0.9 % (<em>p</em> = 0.0001). Multivariate analysis revealed that the risk factors for developing a bone mineral density ≤ −2 SD 2 years after bariatric surgery in patients with normal BMD before surgery were age and lower lean and fat mass before the surgery (OR = 1.07, 95%CI = [1.03–1.12], OR = 0.83, 95%CI = [0.77–0.91], OR = 1.08, 95%CI = [1.02–1.15], respectively).</p></div><div><h3>Conclusion</h3><p>There was a significantly higher prevalence of osteoporosis and low bone mass 2 years after bariatric surgery. Older age and lower lean and fat mass at baseline were risk factors for the development of a BMD ≤ -2SD at 2 years.</p></div>","PeriodicalId":9043,"journal":{"name":"Bone Reports","volume":"22 ","pages":"Article 101782"},"PeriodicalIF":2.1,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352187224000494/pdfft?md5=2af139fe90844d4d4ac4f8c2a4bff14b&pid=1-s2.0-S2352187224000494-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141481970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radiofrequency echographic multi-spectrometry-based measurement of bone mineral density in patients with severe motor and intellectual disability: An opportunity for patients with severe scoliosis and hip dislocation","authors":"Tomoko Sakai ,&nbsp;Masanobu Hirao ,&nbsp;Yusuke Takashina ,&nbsp;Ryo Kitagawa ,&nbsp;Tsutomu Oishi","doi":"10.1016/j.bonr.2024.101781","DOIUrl":"https://doi.org/10.1016/j.bonr.2024.101781","url":null,"abstract":"<div><h3>Purpose</h3><p>Radiofrequency echographic multi-spectrometry (REMS) is an ultrasound technology currently used for the densitometric evaluation of osteoporosis and has been validated against dual-energy X-ray absorptiometry. However, the use of REMS for bone densitometry in patients with severe motor and intellectual disabilities (SMID) remains to be reported. This study aimed to investigate whether REMS technology can be used for densitometric evaluation of osteoporosis in patients with SMID with hip dislocation and severe scoliosis.</p></div><div><h3>Methods</h3><p>Sixty-five patients with SMID, who resided in a long-term care facility and received comprehensive medical and rehabilitation care, underwent REMS scans of the femoral neck and/or lumbar spine. Data regarding anthropometric parameters (height and weight), bone mineral density (BMD), clinical diagnostic classification, physical ability, presence of scoliosis and hip dislocation, and frontal radiographs of both hip joints were obtained.</p></div><div><h3>Results</h3><p>We included 29 men and 34 women (mean age: 52.6 years). All patients underwent successful scanning at either the femoral neck (82.5 %) or lumbar spine (95.2 %). BMD measurements obtained using REMS revealed low values, with a mean BMD, T-score, and <em>Z</em>-score of 0.67 g/cm², −2.39 standard deviation (SD), and − 1.38 SD, respectively, at the femoral neck and 0.66 g/cm², −2.70 SD, and − 1.87 SD, respectively, at the lumbar spine. The average Cobb angle of the lumbar spine was 34.0°; furthermore, dislocation rates did not significantly differ between those with and without successful BMD measurements (<em>p</em> = 0.073). Lumbar BMD T-scores were significantly correlated with femoral neck BMD T-scores (<em>p</em> &lt; 0.001, <em>r</em> = 0.530).</p></div><div><h3>Conclusion</h3><p>All patients with SMID were able to undergo measurements of either spinal or femoral neck BMD; furthermore, 77.7 % of the patients underwent measurements at both the lumbar spine and femur. Our data suggest that REMS is useful for measuring BMD in patients with SMID who are residing in institutions.</p></div>","PeriodicalId":9043,"journal":{"name":"Bone Reports","volume":"22 ","pages":"Article 101781"},"PeriodicalIF":2.1,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352187224000482/pdfft?md5=a345bb9a2711acf85ee72d7cf3ff622e&pid=1-s2.0-S2352187224000482-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141486160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activation of Wnt signaling in human fracture callus and nonunion tissues","authors":"Michael Hadjiargyrou ,&nbsp;Maria Kotsiopriftis ,&nbsp;Dominique Lauzier ,&nbsp;Reggie C. Hamdy ,&nbsp;Peter Kloen","doi":"10.1016/j.bonr.2024.101780","DOIUrl":"https://doi.org/10.1016/j.bonr.2024.101780","url":null,"abstract":"<div><p>The Wnt signaling pathway is a key molecular process during fracture repair. Although much of what we now know about the role of this pathway in bone is derived from in vitro and animal studies, the same cannot be said about humans. As such, we hypothesized that Wnt signaling will also be a key process in humans during physiological fracture healing as well as in the development of a nonunion (hypertrophic and oligotrophic). We further hypothesized that the expression of Wnt-signaling pathway genes/proteins would exhibit a differential expression pattern between physiological fracture callus and the pathological nonunion tissues. We tested these two hypotheses by examining the mRNA levels of key Wnt-signaling related genes: ligands (WNT4, WNT10a), receptors (FZD4, LRP5, LRP6), inhibitors (DKK1, SOST) and modulators (CTNNB1 and PORCN). RNA sequencing from calluses as well as from the two nonunion tissue types, revealed that all of these genes were expressed at about the same level in these three tissue types. Further, spatial expression experiments identified the cells responsible of producing these proteins. Robust expression was detected in osteoblasts for the majority of these genes except SOST which displayed low expression, but in contrast, was mostly detected in osteocytes. Many of these genes were also expressed by callus chondrocytes as well. Taken together, these results confirm that Wnt signaling is indeed active during both human physiological fracture healing as well as in pathological nonunions.</p></div>","PeriodicalId":9043,"journal":{"name":"Bone Reports","volume":"22 ","pages":"Article 101780"},"PeriodicalIF":2.5,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352187224000470/pdfft?md5=5849fc4b34d3d280b9e9ffa30686a7fc&pid=1-s2.0-S2352187224000470-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141429715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pseudotumoral calcinosis of the elbow with ulnar and radial nerve deficits","authors":"Marie-Charlotte Trojani ,&nbsp;Marc-Olivier Gauci ,&nbsp;Caroline Cointat ,&nbsp;Laura Cabane ,&nbsp;Véronique Breuil","doi":"10.1016/j.bonr.2024.101779","DOIUrl":"10.1016/j.bonr.2024.101779","url":null,"abstract":"<div><p>We report here a rare case of an acute peripheral nerve compression by pseudotumoral calcinosis (PCT) at the right elbow in a patient with severe tertiary hyperaparathyroidism. This complication required urgent multidisciplinary management. Surgical decompression by PCT resection enabled rapid motor and sensory recovery.</p></div>","PeriodicalId":9043,"journal":{"name":"Bone Reports","volume":"22 ","pages":"Article 101779"},"PeriodicalIF":2.5,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352187224000469/pdfft?md5=70fcd3af4cba9e18f8fb187fc445c851&pid=1-s2.0-S2352187224000469-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141394427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Skeletal abnormalities, pediatric-onset severe osteoporosis, and multiple fragility fractures in a patient with a novel CTNNB1 de novo variant","authors":"Olga Lesnyak ,&nbsp;Francesca Marini ,&nbsp;Polina Sokolnikova ,&nbsp;Margarita Sorokina ,&nbsp;Kseniya Sukhareva ,&nbsp;Irina Artamonova ,&nbsp;Vladimir Kenis ,&nbsp;Olga Tkach ,&nbsp;Anna Kostareva ,&nbsp;Maria Luisa Brandi","doi":"10.1016/j.bonr.2024.101777","DOIUrl":"https://doi.org/10.1016/j.bonr.2024.101777","url":null,"abstract":"<div><p>We report a case of a patient with a <em>de novo</em> germline heterozygous truncating variant of <em>CTNNB1</em> gene (c.2172del, p.Tyr724Ter) causing neurodevelopmental disorder with spastic diplegia and visual defects syndrome (NEDSDV) associated with a new clinical feature — severe pediatric-onset osteoporosis and multiple fractures. A functional effect of the identified variant was demonstrated using adipose-tissue derived primary mesenchymal stem cells, where we detected the alteration of <em>CTNNB1</em>mRNA and β-catenin protein levels using real-time PCR and Western blot analysis.</p></div>","PeriodicalId":9043,"journal":{"name":"Bone Reports","volume":"21 ","pages":"Article 101777"},"PeriodicalIF":2.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352187224000445/pdfft?md5=54d3edbd74b98ae614c3cd3553c98985&pid=1-s2.0-S2352187224000445-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141249504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eggshell membrane as promising supplement to maintain bone health: A systematic review","authors":"Johannes-Paul Fladerer ,&nbsp;Selina Grollitsch","doi":"10.1016/j.bonr.2024.101776","DOIUrl":"10.1016/j.bonr.2024.101776","url":null,"abstract":"<div><p>Bone loss is a well-known phenomenon in the older population leading to increased bone fracture risk, morbidity, and mortality. Supplementation of eggshell membrane (ESM) is evaluated due to its possible application to prevent bone loss and usage in osteoporosis therapy. The similar organic chemical composition of ESM and human bone is described in detail as both mainly consist of collagen type I, chondroitin sulfate, dermatan sulfate, hyaluronic acid and elastan. ESM and its components are reported to improve mineralization in bone tissue. In many studies ESM intake reduced pain in patients with joint disorders and reduced inflammatory processes. Additionally, ESM improved calcium uptake in human cells. These findings in comparison with a clinical pilot study reporting pain reduction in osteoporotic patients and increased osteoblast activity in in vitro assays support ESM to be a beneficial supplement for bone health. In this systematic review we combined chemical structure analysis with clinical studies to give a more comprehensive picture with novel explanations.</p></div>","PeriodicalId":9043,"journal":{"name":"Bone Reports","volume":"21 ","pages":"Article 101776"},"PeriodicalIF":2.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352187224000433/pdfft?md5=b746c3e0bddae5ba111a0a4371169f5c&pid=1-s2.0-S2352187224000433-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141133839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of medication treatment for women with osteoporosis: A real-world retrospective study from Chinese tertiary grade A hospital","authors":"Xiaolei Liu ,&nbsp;Zhenling Zhu ,&nbsp;Xianli Wang","doi":"10.1016/j.bonr.2024.101778","DOIUrl":"https://doi.org/10.1016/j.bonr.2024.101778","url":null,"abstract":"<div><h3>Purpose</h3><p>This study aimed to analyze the current medication treatment status for women with osteoporosis (OP) based on real-world prescription data from 2016 to 2021 in Chinese nine cities' tertiary Grade A hospital and systematically describe the medication treatment patterns in women with OP.</p></div><div><h3>Methods</h3><p>Prescription information for female OP patients in nine cities (Beijing, Shanghai, Guangzhou, Hangzhou, Tianjin, Zhengzhou, Chengdu, Shenyang, Harbin) was extracted from the Hospital Prescription Analysis Collaboration Project Database of the Hospital Pharmacy Professional Committee of the Chinese Pharmaceutical Association. Statistical analysis was conducted to evaluate demographic characteristics and medication treatment patterns.</p></div><div><h3>Results</h3><p>A total of 669,505 prescriptions for medication treatment of female OP patients were included in this study. The majority of patients were aged 60 to 99 years (69.79 %) followed by 50 to 59 years (18.81 %) and 40 to 49 years (6.69 %). Geographically, the highest concentration of patients was in North China (Beijing, Tianjin) (43.05 %) followed by East China (Shanghai, Hangzhou) (31.43 %). The top three prescribed medications were active vitamin D and its analogs (40.78 %), calcium supplements (32.51 %), and bisphosphonates (18.75 %). The prescription frequency of menopausal hormone therapy (MHT) was 0.31 %. The proportion of female OP patients receiving monotherapy and two drug combinations therapy is equivalent (about 37 %).</p></div><div><h3>Conclusion</h3><p>The diagnosis and treatment of female OP patients in China showed regional variations. The most commonly prescribed medications for this population were calcitriol, calcium carbonate with vitamin D3, and alendronate sodium with vitamin D3. The use of MHT was relatively limited.</p></div>","PeriodicalId":9043,"journal":{"name":"Bone Reports","volume":"21 ","pages":"Article 101778"},"PeriodicalIF":2.5,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352187224000457/pdfft?md5=daf2c9d299476053c5e1df742a44ea63&pid=1-s2.0-S2352187224000457-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141297991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic dosage of isotretinoin in rats may influence orthodontic tooth movement","authors":"Mayra Fernanda Ferreira ,&nbsp;Alberto Carlos Botazzo Delbem ,&nbsp;Edilson Ervolino ,&nbsp;Luy de Abreu Costa ,&nbsp;Cristina Antoniali Silva ,&nbsp;José Ricardo Prando dos Santos ,&nbsp;Marcos Rogério de Mendonça","doi":"10.1016/j.bonr.2024.101775","DOIUrl":"https://doi.org/10.1016/j.bonr.2024.101775","url":null,"abstract":"<div><p>Objective Isotretinoin, also known as 13-cis-retinoic acid, is an isomer of tretinoin, the oxidized form of Vitamin A. Orthodontic tooth movement (OTM) is the result of a cascade of inflammatory responses stimulated by a physical element that is the force generated by orthodontic appliances. Isotretinoin is mainly used among adolescents and young adults, and coincidentally it is this age group that also undergoes orthodontic treatment. Materials and Methods Fifty-five animals were used, and they were randomly divided into 11 groups, containing 5 animals in each group. Group 1: Control; Group 2: OTM for 7 days; Group 3: OTM for 14 days; Group 4: Treated with isotretinoin for 14 days with a dosage of 7.5 mg/kg/day; Group 5: Treated with isotretinoin for 14 days with a dosage of 1.0 mg/kg/day; Group 6: Treated with isotretinoin for 21 days with a dosage of 7.5 mg/kg/day; Group 7: Treated with isotretinoin for 21 days with a dosage of 1.0 mg/kg/day; Group 8: Treated with isotretinoin for 14 days with a dosage of 7.5 mg/kg/day and undergoing OTM for 7 days; Group 9: Treated with isotretinoin for 14 days with a dosage of 1.0 mg/kg/day and undergoing OTM for 7 days; Group 10: Treated with isotretinoin for 21 days with a dosage of 7.5 mg/kg/day and undergoing OTM for 14 days; Group 11: Treated with isotretinoin for 21 days with a dosage of 1.0 mg/kg/day and undergoing OTM for 14 days. In Groups 8, 9, 10 and 11, the animals were treated with isotretinoin for 7 days before OTM and maintained during the movement period in the respective groups.</p><p>Results There was a significant difference in microtomographic parameters, including Trabecular Volume (BV/TV), Trabecular Thickness (Tb.Th), Number of Trabeculae (Tb.N), and Trabecular Separation (Tb.Sp), between the groups. The group that received orthodontic force in conjunction with isotretinoin treatment at a dosage of 7.5 mg/kg/day exhibited lower tooth displacement over a period of 21 days and 14 days. Conclusion Isotretinoin caused a reduction in tooth displacement during OTM when administered at a dose of 7.5 mg/kg/day and isotretinoin did change the microtomographic parameters of treated animals.</p></div>","PeriodicalId":9043,"journal":{"name":"Bone Reports","volume":"21 ","pages":"Article 101775"},"PeriodicalIF":2.5,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352187224000421/pdfft?md5=ef156b594461ac255577f4c51b821958&pid=1-s2.0-S2352187224000421-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141067088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bone collagen tensile properties of the aging human proximal femur","authors":"Stefan Bracher ,&nbsp;Benjamin Voumard ,&nbsp;Mathieu Simon ,&nbsp;Tatiana Kochetkova ,&nbsp;Michael Pretterklieber ,&nbsp;Philippe Zysset","doi":"10.1016/j.bonr.2024.101773","DOIUrl":"https://doi.org/10.1016/j.bonr.2024.101773","url":null,"abstract":"<div><p>Despite the dominant role of bone mass in osteoporotic fractures, aging bone tissue properties must be thoroughly understood to improve osteoporosis management. In this context, collagen content and integrity are considered important factors, although limited research has been conducted on the tensile behavior of demineralized compact bone in relation to its porosity and elastic properties in the native mineralized state. Therefore, this study aims (i) at examining the age-dependency of mineralized bone and collagen micromechanical properties; (ii) to test whether, and if so to which extent, collagen properties contribute to mineralized bone mechanical properties.</p><p>Two cylindrical cortical bone samples from fresh frozen human anatomic donor material were extracted from 80 proximal diaphyseal sections from a cohort of 24 female and 19 male donors (57 to 96 years at death). One sample per section was tested in uniaxial tension under hydrated conditions. First, the native sample was tested elastically (0.25 % strain), and after demineralization, up to failure. Morphology and composition of the second specimen was assessed using micro-computed tomography, Raman spectroscopy, and gravimetric methods. Simple and multiple linear regression were employed to relate morphological, compositional, and mechanical variables with age and sex.</p><p>Macro-tensile properties revealed that only elastic modulus of native samples was age dependent whereas apparent elastic modulus was sex dependent (<em>p</em> &lt; 0.01). Compositional and morphological analysis detected a weak but significant age and sex dependency of relative mineral weight (<em>r</em> = −0.24, <em>p</em> &lt; 0.05) and collagen disorder ratio (I_∼1670/I_∼1640, <em>r</em> = 0.25, p &lt; 0.05) and a strong sex dependency of bone volume fraction while generally showing consistent results in mineral content assessment. Young's modulus of demineralized bone was significantly related to tissue mineral density and Young's modulus of native bone.</p><p>The results indicate that mechanical properties of the organic phase, that include collagen and non-collagenous proteins, are independent of donor age. The observed reduction in relative mineral weight and corresponding overall stiffer response of the collagen network may be caused by a reduced number of mineral-collagen connections and a lack of extrafibrillar and intrafibrillar mineralization that induces a loss of waviness and a collagen fiber pre-stretch.</p></div>","PeriodicalId":9043,"journal":{"name":"Bone Reports","volume":"21 ","pages":"Article 101773"},"PeriodicalIF":2.5,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352187224000408/pdfft?md5=b8a6e5c1f670a144e7a148a0373fd890&pid=1-s2.0-S2352187224000408-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140947972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}