Biosciences, Biotechnology Research Asia最新文献_第9页

Potential of Molecular Plant Breeding for Sustaining the Global Food Security 分子植物育种对维持全球粮食安全的潜力

Biosciences, Biotechnology Research Asia Pub Date : 2023-03-30 DOI: 10.13005/bbra/3072

R. Mahajan, Nisha Kapoor

引用次数: 0

In Silico Prediction of Antidiabetic Activity of Phytoconstituents of Pterocarpus Marsupium Targeting α-Amylase Enzyme 以α-淀粉酶为靶点的有袋翼果植物成分抗糖尿病活性的计算机预测

Biosciences, Biotechnology Research Asia Pub Date : 2023-03-30 DOI: 10.13005/bbra/3077

K. Danao, Shruti Kale, Vijayshri Rokde, Deweshri Nandurkar, U. Mahajan, Nitin G Dumore, Atul R. Bendale, V. Naphade, A. Tatode

{"title":"In Silico Prediction of Antidiabetic Activity of Phytoconstituents of Pterocarpus Marsupium Targeting α-Amylase Enzyme","authors":"K. Danao, Shruti Kale, Vijayshri Rokde, Deweshri Nandurkar, U. Mahajan, Nitin G Dumore, Atul R. Bendale, V. Naphade, A. Tatode","doi":"10.13005/bbra/3077","DOIUrl":"https://doi.org/10.13005/bbra/3077","url":null,"abstract":"ABSTRACT: Background Diabetes is characterized by a metabolic imbalance of blood sugar levels. α-amylase enzyme hydrolyzed starch into glucose units. Current therapy has significant side effects. Current investigation of in silico antidiabetic evaluation of phytoconstituents of Pterocarpus marsupium targeting α-amylase. Methods In silico studies were investigated to determine the binding affinity of phytoconstituents of Pterocarpus marsupium in additional with the crystal structure of α-amylase (PDB ID: 3BC9) with help of Pyrx in autodock vina software. Further, investigate the amino acid interaction residue and impacts on the inhibitory potential of the active phytoconstituents. Additionally, the pharmacokinetics and SwissADME and pkCSM were used as online servers for the toxic effects research. Further, studied the pocket region of amino acid for the binding of phytoconstituents using the Ramachandran plot. Result Molecular docking results proposed that pterostilbenes and liquirtigenin (-8.1 kcal/mol) had best docked against α-amylase as related to native ligand (-5.6 kcal/mol) and metformin (-5.3 kcal/mol). The active phytoconstituent has actively participated in interaction with the amino acid residue leads to blockage of α-amylase activity. Furthermore, the pharmacokinetic and In ADMET investigations, the phytoconstituents toxicological values are within allowable ranges. Conclusion The most promising outcome was revealed by the phytoconstituents of Pterocarpus marsupium that bind to α -amylase. However, it encourages the traditional practice of Pterocarpus marsupium and delivers vital information in drug development and clinical treatment. It promotes traditional approach of Pterocarpus marsupium and provides crucial knowledge for medical research and therapeutic care.","PeriodicalId":9032,"journal":{"name":"Biosciences, Biotechnology Research Asia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89118538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effect of Handling Stress on Primary and Secondary Stress Responses of the Catfish, Clarias batrachus 处理应激对鲶鱼主、次应激反应的影响

Biosciences, Biotechnology Research Asia Pub Date : 2023-03-30 DOI: 10.13005/bbra/3076

Shifali Hafeez, F. A. Sherwani

引用次数: 0

Qualitative Analysis of Culturable Gut Microbes of Selected Cephalopod Species- A Comparative Study 选定头足类动物可培养肠道微生物的定性分析-比较研究

Biosciences, Biotechnology Research Asia Pub Date : 2023-03-30 DOI: 10.13005/bbra/3082

{"title":"Qualitative Analysis of Culturable Gut Microbes of Selected Cephalopod Species- A Comparative Study","authors":"","doi":"10.13005/bbra/3082","DOIUrl":"https://doi.org/10.13005/bbra/3082","url":null,"abstract":"ABSTRACT: Microbes present in the digestive tract of an organism are of great importance. The microbial qualitative and quantitative composition represents the physical, physiological conditions, habits, habitats of the organisms as well as their association patterns. The microbes of digestive system have prominent role not only in the sustainability of the organism but also in the food processing industry. The marine cephalopods are one of the preferred marine food resources, next to marine fisheries. The current study aims to understand the microbial content in the digestive system of consumable cephalopods such as sepia and cuttlefish. The commercially available squids from the market and cuttlefish samples from fishing area were collected and used for this study. The organisms were dissected in aseptic conditions and bacteria collected from the guts of these animals. Culturable bacteria were isolated and, identified using gram characteristics as well as 16s r RNA techniques based molecular identification. The identified bacteria were reported to Gen Bank submission. Bacterial representation in the gut microbiome of cephalopods is low and showed distinct difference between cuttlefish and squid species. This low number of bacterial composition may be due to the habitat conditions or the association of bacteria with the animal requires further studies to understand. The commercial value of cephalopods as protein rich food prioritizes the need to address proper treatment process which can alleviate their presence in Indian food industry scenario. The inter and intra-species relation of microbes and metazoans and the associated macromolecules can be used for pharma industry also in future","PeriodicalId":9032,"journal":{"name":"Biosciences, Biotechnology Research Asia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88335095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purification and Structural Characterization of an Antimicrobial Compound, Lipoxazolidinone a Produced by a Lactobacillus Apis YMP3 api乳酸杆菌YMP3抗菌化合物脂酰唑烷酮a的纯化及结构表征

Biosciences, Biotechnology Research Asia Pub Date : 2023-03-30 DOI: 10.13005/bbra/3090

D. Kumar, Palanisamy Venkatachalam

{"title":"Purification and Structural Characterization of an Antimicrobial Compound, Lipoxazolidinone a Produced by a Lactobacillus Apis YMP3","authors":"D. Kumar, Palanisamy Venkatachalam","doi":"10.13005/bbra/3090","DOIUrl":"https://doi.org/10.13005/bbra/3090","url":null,"abstract":"ABSTRACT: Strains of Vibrio cholerae are one among the most causative and serious disease causing human pathogenic agents, its infections are caused mostly by ingesting contaminated water and/or food. According to the recent estimates, between 1.3 and 4.0 million individuals are infected all around the world every year. The lactic acid bacteria are an important class of probiotics microbes have their ability to produce diversified bioactive compounds, hence this study focused on the identification of a promising antimicrobial agent from a Lactobacillus apis YMP3. This strain was cultured on MRS broth and the cell free supernatant was ethyl acetate extracted for the antimicrobial agent. The crude extract was further purified with C18 silica gel column chromatography and structurally characterized by FT-IR, NMR, GC and MS/MS spectrum. The chemistry of the compound was confirmed as Lipoxazolidinone A which has the IUPAC name of (2E)-5-butyl-2-[(E)-4-methyl-2-oxoundec-3-enylidene]-1,3-oxazolidin-4-one. This is the first report of Lipoxazolidinone A produced by a bacterium, L. apis YMP3 which was originally isolated from yoghurt. This finding expands the scope of identifying more promising bioactive compounds from probiotic Lactobacillus sp., further, this systematic procedure for purification of this antimicrobial agent stood as the baseline data for more elaborate therapeutic studies in future.","PeriodicalId":9032,"journal":{"name":"Biosciences, Biotechnology Research Asia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79191195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Advancements and Mechanism of Plastics Biodegradation Promoted by Bacteria: A Key for Sustainable Remediation for Plastic Wastes 细菌促进塑料生物降解的研究进展及机理:塑料垃圾可持续修复的关键

Biosciences, Biotechnology Research Asia Pub Date : 2023-03-30 DOI: 10.13005/bbra/3063

K. Kaur, Samiksha Sharma, Nidhi Shree, Rekha Mehrotra

{"title":"Recent Advancements and Mechanism of Plastics Biodegradation Promoted by Bacteria: A Key for Sustainable Remediation for Plastic Wastes","authors":"K. Kaur, Samiksha Sharma, Nidhi Shree, Rekha Mehrotra","doi":"10.13005/bbra/3063","DOIUrl":"https://doi.org/10.13005/bbra/3063","url":null,"abstract":"ABSTRACT: Plastic has become an indispensable part of our lives and cutting down plastic consumption entirely is difficult to achieve. The recalcitrant and non-biodegradable nature of plastic leads to accumulation of tons of plastic in landfills and water bodies which further risks marine life and human life too causing serious health issues. In recent years, several microbial enzymes have been discovered that have the ability to degrade plastic. The present review highlights the recent discovery and properties of the plastic-eating bacteria, Ideonella sakaiensis, that has potential to be used for plastic degradation and recycling. The bacteria possess unique enzymes that allow it to utilise Polyethylene terephthalate (PET) plastic, thereby degrading it to relatively safer monomeric forms that can be further degraded and purified to manufacture recycled plastics. The review focuses on the mechanism of PET hydrolysis, recent advances in the field to escalate enzymatic efficiency and development of new bacterial and enzymatic strains through genetic engineering which can enhance its catalytic competence and make the process time and cost-effective. The plastic metabolising bacteria can thus be a potential and efficient bio-alternative to degrade plastic in a biological and sustainable manner thereby helping scale the otherwise insurmountable plastic pollution crisis.","PeriodicalId":9032,"journal":{"name":"Biosciences, Biotechnology Research Asia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78402719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Assessment of Risk Factors for Developing Vulvovaginal Candidiasis Among Women at Various Age Groups 不同年龄组妇女发生外阴阴道念珠菌病的危险因素评估

Biosciences, Biotechnology Research Asia Pub Date : 2023-03-30 DOI: 10.13005/bbra/3095

Irene V.R, C. Sajeeth, V. Karthikeyan, Sabitha J Sabitha J

{"title":"Assessment of Risk Factors for Developing Vulvovaginal Candidiasis Among Women at Various Age Groups","authors":"Irene V.R, C. Sajeeth, V. Karthikeyan, Sabitha J Sabitha J","doi":"10.13005/bbra/3095","DOIUrl":"https://doi.org/10.13005/bbra/3095","url":null,"abstract":"ABSTRACT: Vulvovaginal candidiasis (VVC) is the second most frequent vaginal infection in women and occurs as an opportunistic infection generally caused by Candida albicans.The objective of the research is to determine the risk factors for developing VVC in women of reproductive age, pregnancy, and postmenopausal age. A 6-month prospective study was carried out in pregnant women, post-menopausal women, and women of reproductive age. In MS Excel 2007, the obtained cases were entered, and descriptive statistics were utilized to calculate the percentage of various parameters.The research consisted of a total of 90 individuals, whose participants ranged in age from 18 to 65. VVC was more common (40%) among females there in the reproductive ages of 18 to 35 (86.7%) of the 90 patients who don't follow regular exercise and 54.4 % have a BMI range (of 25-29.9) Overweight. Obesity, past medical and medication history such as Diabetes mellitus (27.7%), and antibiotic usage (23.3%) were major risk factors for acquiring VVC. Co-morbidities like diabetic mellitus, hypothyroidism, urinary tract infections, obesity, as well as PCOD are the main risk factors to cause VVC. Antibiotic usage in the past, the consumption of steroids, and an absence of regular exercise all contribute to the emergence of VVC.","PeriodicalId":9032,"journal":{"name":"Biosciences, Biotechnology Research Asia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77812912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nutritional and Sensory Characterization of Watermelon Rind Powder Incorporated Crackers 西瓜皮粉复合脆饼的营养和感官特性

Biosciences, Biotechnology Research Asia Pub Date : 2023-03-30 DOI: 10.13005/bbra/3087

R. A. R. Arivuchudar

引用次数: 0

Anethum Graveolens L. Microgreen Incorporated Pulse-Based Gluten-Free Crackers: A Potential Functional Snack 微绿结合了基于脉冲的无麸质饼干:一种潜在的功能性零食

Biosciences, Biotechnology Research Asia Pub Date : 2023-03-30 DOI: 10.13005/bbra/3092

K. P G, Subaratinam R Subaratinam R

{"title":"Anethum Graveolens L. Microgreen Incorporated Pulse-Based Gluten-Free Crackers: A Potential Functional Snack","authors":"K. P G, Subaratinam R Subaratinam R","doi":"10.13005/bbra/3092","DOIUrl":"https://doi.org/10.13005/bbra/3092","url":null,"abstract":"ABSTRACT: Crackers were formulated with pulse flours – Chickpea and Pea along with the incorporation of Anethum Graveolens L. microgreens to develop an end product that is rich in protein, fibre, and micronutrients, gluten-free and low in glycemic index. Microgreens are tiny greens that are often harvested in an immature state that are loaded with innumerable benefits thus, Anethum Graveolens L. was utilized as a part of the study. The main goal was to develop a gluten-free snack that was rich in micronutrients due to the lack of availability of such products in the market. The crackers were inflicted to different analyses that included – sensory analysis, physicochemical analysis, proximate analysis, antioxidant analysis (DPPH Radical Scavenging Activity-DRSA and Ferric Reducing Antioxidant Power- FRAP) , texture analysis, microbial analysis, and shelf-life analysis. Results exhibited that the crackers had a remarkable consumer acceptance, were nutrient-dense, had potential antioxidant activity, minimal microbial activity, and had an extended shelf-life. The crackers weighed 3.0±1.0g per unit and the average pH of the crackers was 6.09±0.34. They were rich in protein (28.4g), dietary fibre (3.99g) micronutrients, high in antioxidants (DRSA-21.4% and FRAP-575.71 µM Fe(II)/g), and had low fat (9.19g) content per 100g of the crackers. Thus, the Anethum graveolens L. microgreen incorporated pulse-based gluten-free crackers are an ideal snack with phenomenal potential.","PeriodicalId":9032,"journal":{"name":"Biosciences, Biotechnology Research Asia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75055769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Formulation and Evaluation of Tea tree/ Rosemary Essential oil-based Microemulsion for Antimicrobial Activity 茶树/迷迭香精油微乳液的制备及抑菌活性评价

Biosciences, Biotechnology Research Asia Pub Date : 2023-03-30 DOI: 10.13005/bbra/3084

Pooja Malik, P. Upadhyay

{"title":"Formulation and Evaluation of Tea tree/ Rosemary Essential oil-based Microemulsion for Antimicrobial Activity","authors":"Pooja Malik, P. Upadhyay","doi":"10.13005/bbra/3084","DOIUrl":"https://doi.org/10.13005/bbra/3084","url":null,"abstract":"ABSTRACT: This research aims to prepare microemulsion from Tea Tree and Rosemary essential oil to treat gram-positive and gram-negative bacterial infections. A phase titration has been used to make the proposed microemulsion, which contained 71% water, 23% of a 1:1 mixture of propylene glycol (surfactant), tween 80 (co-surfactant), and 6% of tea tree/rosemary essential oil. In Rosemary essential oil pale yellowish transparent microemulsion, droplet size, zeta potential, and polydispersity index (PDI) of 58.9, 883.6, and 0.678, respectively, showed a stable microemulsion. Tea tree oil loaded in a light, clear microemulsion with zeta potential, droplet size, and polydispersity index (PDI) values of 265.9, 746.8, and 0.987, respectively, indicated a stable microemulsion. The RMO/TTO micro emulsion's viscosity was 1.844/1.933 cps, and its pH ranged from 4.69 to 5.59. The formulation was determined to be a safe, reliable, and effective one in light and good in stability experiments. Escherichia coli, Staphylococcus aureus, and Candida albicans can all be treated using a microemulsion that contains 6% tea tree oil and rosemary oil by agar well diffusion method. Both the EOs-based microemulsion showed more zone of inhibition against Escherichia coli and Candida albicans while Staphylococcus aureus is the less susceptible zone of inhibition. The microemulsion formulation's minimum inhibitory concentration was effective against Staphylococcus aureus, Candida albicans, and E. coli on all physiological parameters. It can be concluded that TTO/RMO essential oil-loaded microemulsion is considered a promising substitute for the current gram-positive and gram-negative regimens.","PeriodicalId":9032,"journal":{"name":"Biosciences, Biotechnology Research Asia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72920297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0