Endocrine disruptors (Austin, Tex.)最新文献

{"title":"Effects of pre- and post-natal exposure to bisphenol A on the stress system","authors":"E. Kitraki, I. Nalvarte, A. Alavian-Ghavanini, J. Rüegg","doi":"10.1080/23273747.2016.1184775","DOIUrl":"https://doi.org/10.1080/23273747.2016.1184775","url":null,"abstract":"ABSTRACT Evidence is accumulating that developmental exposure to Bisphenol A (BPA), an abundant endocrine disrupting chemical (EDC), affects neurodevelopment and later brain function, including cognitive performance, social and affective behaviors. However, an impact of BPA exposure on the stress system regulators has only recently come into focus. This mini-review aims to summarize and discuss the available findings from animal studies regarding BPA effects on the stress system and the hypothalamic-pituitary- adrenal (HPA) axis in particular. It appears that perinatal exposure of rodents to low doses of BPA, around the human tolerable daily intake, can modify the levels, expression and epigenetic patterning of key stress mediators at later-life stages in a sex-specific way. These observations provide evidence that developmental exposure to one of the most highly produced chemicals today may contribute to the regulation of the stress axis. Given the importance of the HPA axis in coordination of the organisms' global homeostasis and its implications in stress-related disorders, further studies are needed to delineate the underling mechanisms of these BPA actions.","PeriodicalId":90159,"journal":{"name":"Endocrine disruptors (Austin, Tex.)","volume":"102 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/23273747.2016.1184775","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60059150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of vehicle mortality following in ovo exposure of Japanese quail (Coturnix japonica) eggs to corn oil, triolein and a fatty acid mix","authors":"Karen M. Dean, Leah D. Baltos, T. Carro, A. Iwaniuk, Meredith E Bohannon, M. Ottinger","doi":"10.1080/23273747.2016.1224022","DOIUrl":"https://doi.org/10.1080/23273747.2016.1224022","url":null,"abstract":"ABSTRACT The use of avian egg bioassays for the determination of embryonic mortality and development effects of toxicant exposure is widespread in ecotoxicology. While these studies have a number of experimental limitations to consider, they offer a rapid, cost effective alternative to maternal feeding studies. In preparing to conduct such studies a number of factors must be taken into consideration, including solubility of the toxicant, dissolution solvent, injection site, volume and incubation position. Species-specific requirements for humidity and position should be considered in order to optimize successful incubation with different species. Japanese quail eggs, were injected prior to incubation with 1µl or 5µl of corn oil, triolein or a fatty acid mix, using air cell or albumen injection. Eggs were incubated according to standard poultry practices or in a prone position to determine if there were any differences in hatching success. Hatching success was reduced in eggs that were injected with 5µl and those incubated in a prone position. The highest rate of hatching success was observed for eggs injected with 1µl of the fatty acid mixture through the air cell.","PeriodicalId":90159,"journal":{"name":"Endocrine disruptors (Austin, Tex.)","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/23273747.2016.1224022","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60058757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bisphenol A alters the self-renewal and differentiation capacity of human bone-marrow-derived mesenchymal stem cells","authors":"A. Strong, David F. B. Miller, A. Buechlein, F. Fang, J. Glowacki, J. Mclachlan, K. Nephew, M. Burow, B. Bunnell","doi":"10.1080/23273747.2016.1200344","DOIUrl":"https://doi.org/10.1080/23273747.2016.1200344","url":null,"abstract":"ABSTRACT Bisphenol A (BPA) is an endocrine disruptor commonly used in industry to manufacture polycarbonate plastics. This endocrine disrupting chemical has been shown to mimic estrogens and has been associated with many serious health conditions. The effects of BPA on bone marrow derived mesenchymal stem cells (BMSCs) were investigated in vitro in the present study. BMSCs were exposed to BPA and assessed for proliferation and self-renewal capacity. The impact of BPA on osteogenic and adipogenic differentiation of BMSCs were also explored. Inhibitor studies were conducted to determine whether BPA alters BMSC biology through estrogen receptor (ER) signaling or MAPK signaling pathways. Although BMSCs treated with BPA demonstrated enhanced proliferation rate, BPA-treated BMSCs demonstrated a reduction in the total number of colony forming units (CFU); however, each CFU was larger (composed of more cells). BPA enhanced adipogenic differentiation of BMSCs, which correlated with increased mRNA expression levels for LEP, PPARG, and LPL following treatment with BPA. The administration of the mitogen-activated protein kinase (MAPK) pathway inhibitor PD184,352 reversed the effects of BPA on proliferation and adipogenesis, whereas the treatment of the cells with ICI182,780 blocked the effects of BPA on self-renewal and adipogenesis. Together, these results suggest that BPA markedly impacts BMSC biology, by enhancing proliferation through MAPK signaling, inhibiting self-renewal through ER signaling, and enhancing adipogenesis through both ER signaling or MAPK signaling.","PeriodicalId":90159,"journal":{"name":"Endocrine disruptors (Austin, Tex.)","volume":"204 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/23273747.2016.1200344","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60058744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diverse sources for endocrine disruption in the wild","authors":"M. Lambert, D. Skelly","doi":"10.1080/23273747.2016.1148803","DOIUrl":"https://doi.org/10.1080/23273747.2016.1148803","url":null,"abstract":"ABSTRACT We are increasingly realizing that diverse human activities are associated with endocrine disruption in wildlife populations. Most field research on endocrine disruption tends to narrowly target particular endocrine disrupting chemicals (EDCs) and contaminant sources as the dominant culprit of observed endocrine disruption. Recently, we and our colleagues assessed patterns of endocrine disruption in metamorphosing frogs in ponds along a forest-suburban land use gradient. In conjunction, we assayed for a diversity of putative of EDCs from an array of possible sources. We found that endocrine disruption in suburban frog populations was associated with a cocktail of different EDCs including classical estrogens, phytoestrogens, and metalloestrogens. These results indicate that endocrine disruption in suburban frogs is likely to be the product of multiple chemicals and sources. Our findings have implications for other systems where endocrine disruption is attributed to a limited set of contaminants or sources without necessarily exploring the contributions of other EDC pathways. Here, we discuss our findings in the context of other field endocrine disruption research and encourage future studies to assess the broader EDC landscape which may be impacting wildlife populations.","PeriodicalId":90159,"journal":{"name":"Endocrine disruptors (Austin, Tex.)","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/23273747.2016.1148803","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60059041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bisphenols: More unnecessary surprises","authors":"K. Thayer, Katherine E Pelch, L. Birnbaum, J. Bucher","doi":"10.1080/23273747.2015.1131032","DOIUrl":"https://doi.org/10.1080/23273747.2015.1131032","url":null,"abstract":"ABSTRACT A recent biomonitoring study “Bisphenol A, Bisphenol S, and 4-Hydroxyphenyl 4-Isoprooxyphenylsulfone (BPSIP) in Urine and Blood of Cashiers” reported on levels of BPA, BPS, and a novel BPS-derivative (BPSIP) in cashiers compared to non-cashiers. Our study was the first to detect BPSIP in humans. In this commentary we discuss our findings in the context of considering bisphenols as a class in health assessments and how technological advances in exposure assessment could be utilized to more efficiently identify emerging chemicals of interest.","PeriodicalId":90159,"journal":{"name":"Endocrine disruptors (Austin, Tex.)","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/23273747.2015.1131032","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60059032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential endocrine disruptor activity of drinking water samples","authors":"Marize de Lm Solano, C. Montagner, C. Vaccari, W. Jardim, J. Anselmo-Franci, Ruither de Og Carolino, João Fl Luvizutto, Gisela de A. Umbuzeiro, J. L. de Camargo","doi":"10.4161/23273747.2014.983384","DOIUrl":"https://doi.org/10.4161/23273747.2014.983384","url":null,"abstract":"Conventional water treatment plants (WTP) do not completely remove contaminants with endocrine activity which may then be present in drinking water (DW). The potential for endocrine disruption of 2 DW samples collected in 2010 and 2012 from a conventional WTP in São Paulo, Brazil was investigated. In vivo assays were conducted with 21-day old female rats exposed to DW extracts for 3- (uterotrophic assay) or 20-days (pubertal assay). The exposure represented a daily ingestion of 2 L, 10 L and 20 L of DW per 60 kg-body weight. Caffeine (5.8 – 21 ug/L), estrone (1 ng/L), atrazine (2.2 – 11.2 ng/L), carbendazim (0.22 ng/L), azoxystrobin (0.23 ng/L), tebuconazole (0.19 ng/L) and imidacloprid (0.88 ng/L) were detected in DW extracts by LC-MS/MS. No increase in uterus wet weight in the uterotrophic assay, and no alteration of vaginal opening in the pubertal assay were observed. However, there were increased absolute blotted uterus weights in animals treated for 3-days with the 3 doses of both DW samples. LH and FSH levels showed significant dose-response increases in the uterotrophic assay using the 2010 DW sample, in association with a significantly increased incidence of vaginal keratinization after the 3-day exposure. The pubertal animals exposed to the 2010 DW had a significant body weight gain and decreased LH at the highest dose. Results suggest that DW samples tested exerted estrogenic and hypothalamic-hypophysis activity alterations in vivo.","PeriodicalId":90159,"journal":{"name":"Endocrine disruptors (Austin, Tex.)","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/23273747.2014.983384","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70554834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gastroschisis and endocrine disruptors","authors":"M. Lubinsky","doi":"10.1080/23273747.2015.1039688","DOIUrl":"https://doi.org/10.1080/23273747.2015.1039688","url":null,"abstract":"Endocrine disruptors have been linked to a variety of human and animal disorders showing ongoing increases in frequency, but analysis is often complicated by retrospective issues of definition and ascertainment. Gastroschisis, a congenital abdominal wall defect with 10 to 20 fold or greater increases over the past half century, has origins linked to an estrogenic thrombophilia, suggesting a relationship to estrogen disruption. With this, a distinct morphology and other features make it particularly suited for retrospective studies. Early reports show ongoing increases starting in the U.S. and North-East Europe during the early 1960s. Exogenous and endogenous factors modify risks, which are primarily related to thrombophilia, although other mechanisms, especially vascular, may have a role. These factors can interact, often vary from study to study, and include geographic, genetic, ethnic and racial influences. Increases in other prenatal defects with similar origins indicate more than just an idiosyncratic effect limited to a single disorder. Findings suggest that a single agent is responsible for increases, although other factors can modify its effects. The same agent may affect other conditions through other mechanisms related to endocrine disruptors, which would be supported by a similar epidemiology. Bisphenol A is a possible candidate in terms of mechanisms, timing, increases, and distribution.","PeriodicalId":90159,"journal":{"name":"Endocrine disruptors (Austin, Tex.)","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/23273747.2015.1039688","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60058928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atrazine disrupts gonadal development in a live-bearing lizard","authors":"L. M. Parsley, E. Wapstra, S. M. Jones","doi":"10.1080/23273747.2015.1006071","DOIUrl":"https://doi.org/10.1080/23273747.2015.1006071","url":null,"abstract":"Atrazine (ATZ) is an endocrine disruptor that increases aromatase activity. In viviparous (live-bearing) vertebrates, embryos may be exposed to endocrine disruptors via the placenta. Studies of the effects of ATZ in viviparous amniotes have focused on rodents, which are relatively insensitive to ATZ: studies on other viviparous amniotes are therefore required. We aimed to determine the effects of gestational exposure to a single dose of ATZ at 10 ppb on gonadal development in a viviparous skink, Niveoscincus metallicus. Pregnant skinks were exposed to ATZ, the synthetic estrogen diethylstilbestrol (DES) (positive control), vehicle solvent or no treatment. Gonads were examined histologically at birth. Females born to ATZ and DES exposed mothers were more likely to exhibit ovaries with abnormal oocytes than were females whose mothers received vehicle solvent or no treatment. Males born to ATZ and DES exposed mothers were equally more likely to exhibit testes devoid of germ cells with reduced organization of seminiferous tubules (ST) compared to males born to mothers receiving vehicle solvent or no treatment. However, ATZ treatment significantly increased the number of male neonates born with testicular lesions compared to males born to mothers in any other group. We conclude that atrazine disrupts gonadal differentiation in the viviparous lizard, N. metallicus. The similar effects of DES and ATZ suggest that the developmental effects of ATZ in N. metallicus reflect increased estrogen signaling. Atrazine should be used with caution as exposure of wildlife to this EDC is likely to have adverse effects on reproductive health.","PeriodicalId":90159,"journal":{"name":"Endocrine disruptors (Austin, Tex.)","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/23273747.2015.1006071","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60058839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polychlorinated biphenyl exposure alters oxytocin receptor gene expression and maternal behavior in rat model","authors":"E. N. Dover, David E. Mankin, H. C. Cromwell, V. Phuntumart, L. Meserve","doi":"10.4161/23273747.2014.979681","DOIUrl":"https://doi.org/10.4161/23273747.2014.979681","url":null,"abstract":"Polychlorinated biphenyl (PCB) is a persistent organic pollutant known to induce diverse molecular and behavioral alterations. Effects of PCB exposure could be transmitted to future generations via changes in behavior and gene expression. Previous work has shown that PCB-exposure can alter social behavior. The present study extends this work by examining a possible molecular mechanism for these changes. Pregnant rats (Sprague-Dawley) were exposed through diet to a combination of non-coplanar (PCB 47 - 2,2′,4,4′-tetrachlorobiphenyl) and coplanar (PCB 77 - 3,3′,4,4′-tetrachlorobiphenyl) congeners. Maternal care behaviors were examined by evaluating the rate and quality of nest building on the last 4 d of gestation and dam/pup interactions on postnatal days 1, 2, 4 and 6. On postnatal day 17, dams were euthanized and hypothalamic tissue was removed for expression analyses of the oxytocin receptor (OXTR) and cytochrome P450 1a1 (Cyp1a1). PCB altered nest building and maternal care behaviors. Specifically, there was a significant increase in time spent in low crouch and high crouch nursing posture on PND 4 and PND 6 respectively. Molecular analysis revealed that PCB exposure upregulated OXTR expression in the hypothalamus of dams. These results provide a possible molecular mechanism for PCB-induced changes in social interactions during early development.","PeriodicalId":90159,"journal":{"name":"Endocrine disruptors (Austin, Tex.)","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/23273747.2014.979681","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70554741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicity and tissue accumulation of 4-nonylphenol in the catfish Heteropneustes fossilis with a note on prevalence of 4-NP in water samples","authors":"G. J. Gautam, R. Chaube, K. Joy","doi":"10.4161/23273747.2014.981442","DOIUrl":"https://doi.org/10.4161/23273747.2014.981442","url":null,"abstract":"4-nonylphenol (4-NP) a persistent and ubiquitous xenoestrogen with endocrine-disrupting activities, is present in varying concentrations in wastewater, ground water, treated drinking water and sediments. It is a biodegradation product of alkylphenolethoxylates used widely as a nonionic surfactant. In the present study, the presence of 4-NP in water samples and its toxicity and tissue accumulation were investigated in an air-breathing catfish Heteropneustes fossilis. The concentration of 4-NP varied between 12.40 ± 1.11 μg/L and 16.29 ± 1.18 μg/L in water samples drawn from river Ganga and river Varuna at 3 different sites that witness high anthropogenic activities or near a city wastewater treatment efflux point. Stored tap water contained 4-NP albeit at a low level (3.9 ± 0.23 μg/L). The median lethal concentrations (LC50) of 4-NP were 1600 μg/L and 1632 μg/L, respectively, in the gonad resting phase and gonad active (preparatory) phase. The concentration of 4-NP added to fish holding water was reduced by 67% over 10 hr of fish introduction suggesting a faster accumulation of the toxicant by fish. In a chronic study spanning 60 days, 4-NP added to the water was accumulated by tissues in a concentration (64 and 160 μg/L) and exposure time (15, 30, 45 and 60 days) – dependent manner. Brain accumulated the highest concentration of 4-NP while muscle showed the lowest uptake. Gill, liver, kidney, ovary and plasma showed intermediate ranges of accumulation. HPLC chromatogram profiles showed that 4-NP was metabolized in all tissues (brain, liver, kidney, gill and ovary) except muscle and plasma. A distinct seasonality of 4-NP was not discerned in this study, probably due to the fact that a steady state level was not reached up to 60 d of exposure. The study concludes that 4-NP is an environmentally relevant toxicant and the catfish has a high tolerance level, which may be due to the fact that 4-NP is metabolized by many tissues during its elimination.","PeriodicalId":90159,"journal":{"name":"Endocrine disruptors (Austin, Tex.)","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/23273747.2014.981442","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70554779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}