British biotechnology journal最新文献_第4页

Genetic Improvement of Saccharomyces boulardii R7 and Generate Suitable Strains for Synthesis and Expression of Recombinant Products 博氏酵母菌R7的遗传改良及重组产物的合成与表达

British biotechnology journal Pub Date : 2016-01-10 DOI: 10.9734/bbj/2016/22927

M. Al-Jailawi, Amer Al-Shekdhaher, Rahem. E. AL-Zaiadi

引用次数: 2

Heterobeltiosis in Wheat (Triticum aestivum L.) F 1 Diallel Crosses under Contrasting Soil-N Conditions 小麦杂种优势的研究土壤-氮对比条件下的f1双列杂交

British biotechnology journal Pub Date : 2016-01-10 DOI: 10.9734/BBJ/2016/21916

A. M. M. A. Naggar, R. Shabana, M. Aleem, M. El-Aleem, Zainab A. El Rashidy, Z. El-Rashidy

引用次数: 1

Production and Characterization of Polyhydroxyalkanoate (PHA) Using Mango Seed Kernel as an Alternative to Glucose 用芒果籽仁替代葡萄糖制备聚羟基烷酸酯(PHA)及其特性研究

British biotechnology journal Pub Date : 2016-01-10 DOI: 10.9734/BBJ/2016/25112

K. Nasir-Naeem, K. Shittu, A. Kabiru

引用次数: 7

Phytochemical, Anti-nutrients and Toxicity Evaluation of Cleome gynandra and Solanum nigrum: Common Indigenous Vegetables in Zimbabwe 辛巴威常见的土生蔬菜金缕子和茄的植物化学、抗营养成分及毒性评价

British biotechnology journal Pub Date : 2016-01-10 DOI: 10.9734/BBJ/2016/25164

Caroline Sango, L. Marufu, C. Zimudzi

{"title":"Phytochemical, Anti-nutrients and Toxicity Evaluation of Cleome gynandra and Solanum nigrum: Common Indigenous Vegetables in Zimbabwe","authors":"Caroline Sango, L. Marufu, C. Zimudzi","doi":"10.9734/BBJ/2016/25164","DOIUrl":"https://doi.org/10.9734/BBJ/2016/25164","url":null,"abstract":"Aim: This study assesses the phytochemical profiles, toxicity and anti-nutrient properties of two indigenous vegetables, Cleome gynandra and Solanum nigrum. Methodology: Aerial parts of S. nigrum and C. gynandra collected from wild populations were extracted in water and methanol solvents using the cold maceration procedure. Chemical tests to identify different phytochemicals and antinutrients were carried out using specified reagents. Toxicity of the plant extracts was tested using the Brine Shrimp (Artemia salina) lethality bioassay procedure. Results: Phytochemical tests indicated presence of alkaloids, steroids, flavonoids, cardiac glycosides, saponins, phenols and tannins in both S. nigrum and C. gynandra. Anthraquinones were only found in C. gynandra, whereas terpenoids and coumarin glycosides were found only in Original Research Article Sango et al.; BBJ, 13(3): 1-11, 2016; Article no.BBJ.25164 2 S. nigrum. The anti-nutrient levels in the vegetables were high; tannins (3.580 and 1.820 mg/ 100 g), oxalates (21.560 and 24.97±0.156 g/100 g), saponins (71 and 42%), alkaloids (15.160 and 3.44%) and phytates (0.099±0.046 and 0.250±0.013%) in S. nigrum and C. gynandra respectively. The Brine Shrimp LC50 values obtained were 0.67 and 1.84 mg/ml for methanol and water extracts of S. nigrum and 1.09 and 4.9 mg/ml for C. gynandra, respectively. Conclusion: The vegetables, C. gynandra and S. nigrum are rich sources of diverse phytochemicals. However, some phytochemicals are present in high levels and might act as anti nutritional factors interfering with absorption of food. C. gynandra is safe for consumption but S. nigrum contains some toxic phytochemicals and should not be taken regularly.","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"13 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Database Analysis of Acidic Proteins from Halophilic Species and Their Corresponding Basic Proteins from Non-halophilic Species 嗜盐物种酸性蛋白与非嗜盐物种相应碱性蛋白的数据库分析

British biotechnology journal Pub Date : 2016-01-10 DOI: 10.9734/bbj/2016/25207

H. Nakashima, K. Homma, Michiko Yamazaki, M. Ishizaki, K. Mawatari

{"title":"Database Analysis of Acidic Proteins from Halophilic Species and Their Corresponding Basic Proteins from Non-halophilic Species","authors":"H. Nakashima, K. Homma, Michiko Yamazaki, M. Ishizaki, K. Mawatari","doi":"10.9734/bbj/2016/25207","DOIUrl":"https://doi.org/10.9734/bbj/2016/25207","url":null,"abstract":"Aims: To reveal which amino acid residues determine whether a protein is acidic or basic between orthologous pairs, acidic proteins from halophilic species and corresponding basic proteins from non-halophilic species were compared. Similarly acidic versus acidic protein pairs, and basic versus basic protein pairs were also analyzed. Place and Duration of Study: Department of Clinical Laboratory Science, Graduate Course of Medical Science and Technology, School of Health Sciences, Kanazawa University, Japan. Methodology: Halobacterium sp. NRC-1 was used as halophilic species and Gram-positive bacterium Bacillus subtilis, and radiation resistant bacterium Deinococcus radiodurans were used as non-halophilic species. The three species were selected because their proteins were closely Original Research Article Nakashima et al.; BBJ, 14(2): 1-12, 2016; Article no.BBJ.25207 2 related each other. The amino acid compositions were compared and the amino acid substitutions were counted for the orthologous protein pairs between Halobacterium and B. subtilis. Similar comparison was done for the proteins between Halobacterium and D. radiodurans. Results: The Asp and Glu residues are determinant whether a protein of Halobacterium sp. NRC-1 is acidic or basic. Amino acid substitutions to increase the Asp residues in the acidic proteins of Halobacterium from the corresponding proteins of non-halophilic species were almost identical whether the corresponding proteins were acidic or basic. This result suggested that the change of protein charges from basic proteins to acidic ones was same as from acidic proteins to acidic ones. The proteins of Halobacterium showed a tendency to have residues with smaller side chain than the proteins of B. subtilis / D. radiodurans.","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"14 1","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial Diversity and Time Divergence of Commonly Cultured Cichlids in Nigeria 尼日利亚常见养殖慈鲷的线粒体多样性和时间分化

British biotechnology journal Pub Date : 2016-01-10 DOI: 10.9734/BBJ/2016/25470

O. Agbebi, C. Echefu, I. Adeosun, A. Ajibade, E. A. Adegbite, A. Adebambo, M. B. Ilori, So Durosaro, A. B. Ajibike

引用次数: 2

Molecular Diversity Analysis of Coat Protein Gene Encoded by Legume Begomoviruses and PCR Assay to Detect Yellow Mosaic Viruses Infecting Soybean in India 豆科begomovirus编码外壳蛋白基因的分子多样性分析及印度大豆黄花叶病毒的PCR检测

British biotechnology journal Pub Date : 2016-01-10 DOI: 10.9734/BBJ/2016/24362

S. Ramesh, B. S. Chouhan, G. Gupta, R. Ramteke, S. Chand, S. M. Husain

引用次数: 13

Antifungal Activity of Camel Faeces with Special Reference to Dermatophytes 骆驼粪便抗真菌活性与皮肤真菌的关系

British biotechnology journal Pub Date : 2016-01-10 DOI: 10.9734/BBJ/2016/24744

E. Suleiman, M. Kabashi, S. Elbashir, A. Elhassan

{"title":"Antifungal Activity of Camel Faeces with Special Reference to Dermatophytes","authors":"E. Suleiman, M. Kabashi, S. Elbashir, A. Elhassan","doi":"10.9734/BBJ/2016/24744","DOIUrl":"https://doi.org/10.9734/BBJ/2016/24744","url":null,"abstract":"Aim: The present study was conducted to evaluate the antifungal activity of camel faeces on some pathogenic fungi. Study Design: This is a descriptive evaluation study. Methodology: Camel faeces was extracted following Harborne method using organic solvents. Organic extracts besides, aqueous extract and ash were screened against clinical isolates using agar-well diffusion and incorporated methods. Parallel experiments were conducted with ketoconazole and nystatin, as positive control whereas; the vehicle solvents were used as negative control. Phytochemical analysis of Camel feaces was carried out following Harborne method. Short Research Article Suleiman et al.; BBJ, 13(2): 1-5, 2016; Article no.BBJ.24744 2 Results: Water and ethanol extracts exerted significant effect on dermatophytes followed by chloroform and hexane extracts compared to the ash which revealed no activity. Aspergillus and Pencillium species were found insensitive to all test extracts where as Candida albicans was found sensitive only to the hexane extract. Sterols and triterpenes were revealed on phytochemical analysis. Discussion: The antifungal activity of camel faeces might be due to the sterols and triterpenes. Conclusion: The study confirms efficacy of camel faeces as natural antifungal agent, and suggests the possibility of employing it for treatment of skin infections, caused by the test pathogens. The present study reveals first report on the use of camel faeces against some pathogenic fungi. Recommendation: Identification and characterization of novel molecules are highly recommended.","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"251 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploration of Phytochemical and Antibacterial Potentiality of Anagallis arvensis L. Extract against Methicillin-Resistant Staphylococcus aureus (MRSA) 刺尾草提取物对耐甲氧西林金黄色葡萄球菌(MRSA)的植物化学及抑菌活性研究

British biotechnology journal Pub Date : 2016-01-10 DOI: 10.9734/BBJ/2016/20505

J. Sharifi‐Rad, S. Hoseini-Alfatemi, A. Miri, M. Sharifi-Rad, M. Sharifi-Rad, M. Hoseini, M. Sharifi-Rad

引用次数: 6

Biotechnological Application of Cassava-Degrading Fungal (CDF) Amylase in Broiler Feed Formulation 木薯降解真菌淀粉酶在肉鸡饲料配方中的生物技术应用

British biotechnology journal Pub Date : 2016-01-10 DOI: 10.9734/bbj/2016/20614

O. Avwioroko, A. Anigboro, N. J. Tonukari

引用次数: 10