Blood vessels最新文献_第5页

Hypernoradrenergic innervation and vascular smooth muscle hyperplastic change. 高去甲肾上腺素能神经支配与血管平滑肌增生性改变。

Blood vessels Pub Date : 1991-01-01 DOI: 10.1159/000158858

R J Head

引用次数: 47

Spatial and temporal resolution of serotonin-induced changes in intracellular calcium in a cultured arterial smooth muscle cell line. 5 -羟色胺诱导的动脉平滑肌细胞系细胞内钙变化的时空分辨率。

Blood vessels Pub Date : 1991-01-01 DOI: 10.1159/000158870

W F Goldman

{"title":"Spatial and temporal resolution of serotonin-induced changes in intracellular calcium in a cultured arterial smooth muscle cell line.","authors":"W F Goldman","doi":"10.1159/000158870","DOIUrl":"https://doi.org/10.1159/000158870","url":null,"abstract":"Ca2+ transients (1-2 microM) evoked by serotonin (5-HT) in cultured A7r5 cells were studied using fura-2 and digital imaging microscopy. Fura-2 was introduced into cells either by incubation with its acetoxymethyl ester analogue fura-2/AM or by transient ATP-induced permeabilization of the sarcolemma such that the free fura-2 entered the cell directly. The distribution of cytoplasmic Ca2+ in unstimulated cells loaded by the former method was heterogeneous, reflecting, in part, separate pools of Ca2+ in the cytosol and sarcoplasmic reticulum (SR). In contrast, the distribution of Ca2+ was uniform in cells loaded with fura-2 by transient permeabilization; this reflected the restriction of fura-2 to the cytosol. Average Ca2+ in these cells was substantially lower than that in fura-2/AM-loaded cells, because SR Ca2+ influences the fura-2 signal from fura-2/AM-loaded cells, but not from cells loaded with free fura-2. The differences in the Ca2+ distribution measured by the two loading methods were also evident during the course of 5-HT-evoked Ca2+ transients. Spatial and temporal resolution of the rising phase of 5-HT-evoked Ca2+ transients in fura-2/AM-loaded cells revealed that the onset of the Ca2+ transients was first manifested as small regions of elevated Ca2+ that subsequently expanded until peak apparent intracellular Ca2+ levels were present in virtually all of the nonnuclear regions of the cells. The rate of rise of Ca2+ varied in different cell regions with the nucleus responding the slowest.","PeriodicalId":9009,"journal":{"name":"Blood vessels","volume":"28 1-3","pages":"252-61"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000158870","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13159383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Release of vasoactive peptides from autonomic and sensory nerves. 自主神经和感觉神经血管活性肽的释放。

Blood vessels Pub Date : 1991-01-01 DOI: 10.1159/000158840

J M Lundberg, A Franco-Cereceda, J S Lacroix, J Pernow

引用次数: 29

Postischemic cerebral blood flow and neuroeffector mechanisms. 脑缺血后血流和神经效应机制。

Blood vessels Pub Date : 1991-01-01 DOI: 10.1159/000158842

R Macfarlane, M A Moskowitz, E Tasdemiroglu, E P Wei, H A Kontos

{"title":"Postischemic cerebral blood flow and neuroeffector mechanisms.","authors":"R Macfarlane, M A Moskowitz, E Tasdemiroglu, E P Wei, H A Kontos","doi":"10.1159/000158842","DOIUrl":"https://doi.org/10.1159/000158842","url":null,"abstract":"The influence of neuroeffector mechanisms in the regulation of postischemic cerebral blood flow was investigated by microsphere determination in 8 cats after chronic unilateral vascular deafferentation by trigeminal ganglionectomy. The animals were subjected to 90 min of reperfusion following 10 min of global ischemia induced by 4-vessel occlusion and systemic hypotension. Cortical hyperemia 30 min after reperfusion was attenuated by up to 48% in cortical gray matter ipsilateral to the side of trigeminal ganglionectomy (p less than 0.01). Axon reflex mechanisms involving the release of neuropeptides from peripheral sensory nerve fibers, such as substance P (SP), calcitonin gene-related peptide (CGRP) and neurokinin A (NKA), mediate this response. SP and NKA cause vasodilation by endothelium-dependent mechanisms (endothelium-dependent relaxing factor), whereas CGRP relaxes vascular smooth muscle by direct receptor interactions. Studies were therefore undertaken to determine the extent to which endothelium-dependent mechanisms mediate the hyperemia following global cerebral ischemia. In 7 intact cats, the postischemic response of pial arterioles to the topical application of acetylcholine (ACh; 10(-7) M), an endothelial-dependent vasodilator, was measured using a closed cranial window technique. Although ACh increased pial arteriolar caliber by 17% under resting conditions, the same dose elicited a vasoconstrictor response (87% of pre-ACh diameter 30 min after reperfusion) for the first 60 min of reperfusion after 10 min of ischemia. ACh-induced vasodilation was restored by 75 min (105%), but was less than control even at 120 min (109 vs. 117%; p less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":9009,"journal":{"name":"Blood vessels","volume":"28 1-3","pages":"46-51"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000158842","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13159386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Contractile and morphologic properties of a saphenous vein after 12 years as an aortocoronary bypass graft. 冠状动脉旁路移植术12年后隐静脉的收缩和形态学特征。

Blood vessels Pub Date : 1991-01-01 DOI: 10.1159/000158880

S. Steen, R. Willén, T. Sjöberg, B. Carlén

引用次数: 5

Nucleotides as cotransmitters in vascular sympathetic neuroeffector transmission. 核苷酸在血管交感神经效应器传递中的共递质作用。

Blood vessels Pub Date : 1991-01-01 DOI: 10.1159/000158839

K Starke, I von Kügelgen, J M Bulloch, P Illes

引用次数: 13

Third International Symposium on Resistance Arteries. Rebild, Skorping, Denmark, May 21-25, 1991. Abstracts. 第三届抗动脉国际研讨会。Rebild, Skorping，丹麦，1991年5月21-25日。摘要。

Blood vessels Pub Date : 1991-01-01

引用次数: 0

Functional and morphologic endothelial damage in rabbit external jugular veins stored in heparinized normal saline. 肝素化生理盐水对兔颈外静脉内皮功能和形态学损伤的观察。

Blood vessels Pub Date : 1991-01-01 DOI: 10.1159/000158897

L B Schwartz, Z S Radic, M K O'Donohoe, R L McCann, E M Mikat, P O Hagen

{"title":"Functional and morphologic endothelial damage in rabbit external jugular veins stored in heparinized normal saline.","authors":"L B Schwartz, Z S Radic, M K O'Donohoe, R L McCann, E M Mikat, P O Hagen","doi":"10.1159/000158897","DOIUrl":"https://doi.org/10.1159/000158897","url":null,"abstract":"Previous studies have demonstrated that vein storage in normal saline leads to significant mechanical morphological, and biochemical aberrations. However, little information is available regarding the functional damage that occurs. The purpose of this study was to evaluate the effect of saline storage on venous smooth muscle and endothelial function. Segments of ten external jugular veins from male New Zealand White rabbits were placed nondistended in either modified Krebs solution at 37 degrees C (Krebs-stored, KS) or heparinized normal saline at room temperature (saline-stored, SS) for 1 h. Segments 4 mm in length were then simultaneously studied in vitro under isometric tension. There was no difference in maximum tension or sensitivity to either bradykinin or histamine. Acetylcholine-induced relaxation in KS segments was not significantly different from relaxation in a historical cohort of nonstored segments (nonstored 87.4 +/- 1.0% vs. KS 84.5 +/- 2.0%; p = NS). However, there were significant attenuations in SS segment endothelium-dependent relaxation in response to both acetylcholine (KS 84.5 +/- 2.0% vs. SS 76.4 +/- 2.7%, p less than 0.02) and adenosine diphosphate (KS 47.9 +/- 2.9% vs. SS 40.6 +/- 3.7%, p less than 0.002). Relaxant responses to sodium nitroprusside (endothelium-independent) were not significantly different in the two groups (KS 94.6 +/- 1.6% vs. SS 95.7 +/- 2.2%; p = NS). Electron microscopic evaluation of SS segments revealed endothelial cell disruption with cellular edema and loss of intact junctions.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":9009,"journal":{"name":"Blood vessels","volume":"28 6","pages":"511-9"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000158897","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12944477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Release of nerve growth factor from cultured aortic smooth-muscle cells. 培养的主动脉平滑肌细胞释放神经生长因子。

Blood vessels Pub Date : 1991-01-01 DOI: 10.1159/000158899

T Ueyama, M Hamada, T Hano, I Nishio, Y Masuyama, A Ooshima

引用次数: 11

Contractile protein interactions in smooth muscle. 平滑肌中的收缩蛋白相互作用。

Blood vessels Pub Date : 1991-01-01 DOI: 10.1159/000158856

J C Rüegg, G Pfitzer

引用次数: 9