BioScience最新文献_第10页

Design and Specificity Test of Specific Primers for Neuroglobin Gene Expression Modulation in Brain Tissue of Rattus norvegicus using qRT-PCR 利用 qRT-PCR 技术设计和测试用于调节鼠脑组织中神经球蛋白基因表达的特异性引物

IF 10.1 1区生物学

BioScience Pub Date : 2023-10-31 DOI: 10.24036/bsc.v7i2.125246

Bintang Fadhil Ramadhan, Muhammad Farikh, Muhammad Naufal Arrafi, Nagra Aulia Valofi, Walidatul Awaliyah, Jessi Rizkanauli Simangungsong, Dini Herisanti, S. Farma

{"title":"Design and Specificity Test of Specific Primers for Neuroglobin Gene Expression Modulation in Brain Tissue of Rattus norvegicus using qRT-PCR","authors":"Bintang Fadhil Ramadhan, Muhammad Farikh, Muhammad Naufal Arrafi, Nagra Aulia Valofi, Walidatul Awaliyah, Jessi Rizkanauli Simangungsong, Dini Herisanti, S. Farma","doi":"10.24036/bsc.v7i2.125246","DOIUrl":"https://doi.org/10.24036/bsc.v7i2.125246","url":null,"abstract":". Neuroglobin (Ngb) is a newly discovered globin that is found in large numbers in neurons. Brain cells are very sensitive to lack of oxygen and can begin to die within five minutes after the oxygen supply is cut off. Hypoxic conditions of brain tissue are ischemic in the area of the bleeding center This study aims to design and test the specificity of the Neuroglobin Rattus norvegicus mRNA gene in silico as a nucleotide capable of reading neuroglobin gene expression. The neuroglobin gene sequence was obtained using a \"nucleotide\" search menu provided by NCBI GenBank and designed using Geneious Prime bioinformatics software. The neuroglobin gene sequence used in this study was Rattus norvegicus mRNA with accession number NM_033359.3|:1-1,773. Synthesized primary pairs are optimized using PCR gradients. PCR products were analyzed by electrophoresis using 1.5% agarose gel, 100 V for 27 minutes. The results obtained one forward primer for Rattus norvegicus neuroglobin (Ngb) which has a length of 20 bases with the order of 5' AGTCTTAGCCTCTCCCCCAG -3' and reverse primer has a length of 20 bases with the order of 5' GTCTACAGAACCACGGCACAcx-3' product size 803 bp. The difference Tm in this pair of primers is 0.9 0C. The gradient PCR results showed the thickest and clearest DNA bands were at 57.7°C. Primers with the best primary criteria for neuroglobin genes were obtained with an amplicon size of 803 bp and an aneealing temperature of 57.7 °C. The design results meet the requirements of good criteria so that the primary candidate design results can be used for the PCR process.","PeriodicalId":9003,"journal":{"name":"BioScience","volume":"16 1","pages":""},"PeriodicalIF":10.1,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139308576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiple Alignment and Primer Design for Groups of Barnacle Organisms and Amphibalanus amphitrite as Biofouling Markers 作为生物污损标记物的藤壶生物和鳗鲡群的多重排列和引物设计

IF 10.1 1区生物学

BioScience Pub Date : 2023-10-31 DOI: 10.24036/bsc.v7i2.125392

Muhammad Farikh, Rahmad Wanizal Pastha, Rezeki Rival Alridho, Aura Zahra Nafisah, Bintang Fadhil Ramadhan, A. Achyar

{"title":"Multiple Alignment and Primer Design for Groups of Barnacle Organisms and Amphibalanus amphitrite as Biofouling Markers","authors":"Muhammad Farikh, Rahmad Wanizal Pastha, Rezeki Rival Alridho, Aura Zahra Nafisah, Bintang Fadhil Ramadhan, A. Achyar","doi":"10.24036/bsc.v7i2.125392","DOIUrl":"https://doi.org/10.24036/bsc.v7i2.125392","url":null,"abstract":". The impact caused by biota biofouling activities is the occurrence of corrosiveness and paralysis in installation buildings. To facilitate the installation and stability of biodiversity, researchers try to present environmental DNA (e-DNA) as environmental samples without killing and isolating an organism by utilizing environmental samples. This study aims to obtain a universal and specific primer of the Cytochrome Oxidase I (COX1) gene that can be used to identify biofouling barnacle groups designed in silico. The sequence used in this study was from Amphibalanus amphitrite mitochondria and Semibalanus balanoides with NCBI accession numbers NC_024525.1|:1-1,551 and NC_039849|:1-1,551. The sequence of COX1 sequences obtained was saved in FASTA format for further use in the primer design process using Geneious Prime. The determination of sustainable areas was determined using Benchling and Geneious Prime in the stages of multiple alignment. Sequence with the best specific primer criteria for COX1 A. amphitrite obtained with a length of 20-22 bases and an amplicon size of 910 bp. The sequence of forward and reverse primer bases were 5'-GAGCTGAACTTGGTCAACCG-3' and 5'GCTCAAAGAAGAGGAGGGCTAT-3'. Sequence with best universal primer criteria of the barnacle group for COX1 were obtained with a length of 24 bases each and an amplicon size of 1,270 bp. The sequence of forward and reverse primer bases were 5'-GACTTCTACCGTTAATGTTAGGAG-3' and 5'- CTATGGTAATGAGGAGGAGTAGTG-3'. The design results meet the requirements of a good primer so that the primer candidate design results can be used for the PCR process.","PeriodicalId":9003,"journal":{"name":"BioScience","volume":"34 1","pages":""},"PeriodicalIF":10.1,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139309491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Potential of Hot Water Sapan Sungai Aro Thermophilic Bacteria Consortium in Producing Bioethanol 热水 Sapan Sungai Aro 嗜热细菌联盟生产生物乙醇的潜力

IF 10.1 1区生物学

BioScience Pub Date : 2023-10-31 DOI: 10.24036/bsc.v7i2.123265

Inayatul Fatia, I. Irdawati, L. Advinda, A. Anhar

{"title":"The Potential of Hot Water Sapan Sungai Aro Thermophilic Bacteria Consortium in Producing Bioethanol","authors":"Inayatul Fatia, I. Irdawati, L. Advinda, A. Anhar","doi":"10.24036/bsc.v7i2.123265","DOIUrl":"https://doi.org/10.24036/bsc.v7i2.123265","url":null,"abstract":". Biofuel is a potentially renewable alternative fuel in Indonesia. Bioethanol is one example of the most commonly used biofuel. Microorganisms of thermophilic bacteria are known to contribute to the production of bioethanol. Thermophilic bacteria are efficient against high temperature conditions so as to minimize contamination. Production of bioethanol can also use joint culture (consortium). Bioethanol production using a microbial biculture consortium is known to significantly increase the level of bioethanol production. The purpose of this study was to determine the compatibility and to determine the optimum potential of the thermophilic bacterial biculture consortium of Sapan Sungai Aro hot water for bioethanol production. This research is a type of descriptive research. To test the cooperation between consortium isolates of thermophilic bacteria producing bioethanol, a compatibility test was carried out using the disk diffusion method. Then the consortium isolates were fermented in liquid TMM (Thermophilic Minimum Media) medium, the bioethanol content was measured after distillation using a pycnometer. The results of the bacterial compatibility test showed that there was one pair of isolates that were not compatible, namely SSA 8 & SSA 14 due to the presence of a clear zone. On research results. The production of bioethanol by a consortium of thermophilic bacteria gives more optimal results compared to a single isolate. The best thermophilic bacterial biculture consortium from the Sapan Sungai Aro hot spring in producing biofuels is SSA 14 & SSA 16 which is 3.009%.","PeriodicalId":9003,"journal":{"name":"BioScience","volume":"5 1","pages":""},"PeriodicalIF":10.1,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139308072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rice Growth Response to Drought Simulation Treatment Using PEG 水稻生长对使用 PEG 进行干旱模拟处理的响应

IF 10.1 1区生物学

BioScience Pub Date : 2023-10-31 DOI: 10.24036/bsc.v7i2.122674

Annisa Khaira, Z. Zulyusri, A. Achyar, Dwi Hilda Putri, Yusni Atifah, V. Violita

{"title":"Rice Growth Response to Drought Simulation Treatment Using PEG","authors":"Annisa Khaira, Z. Zulyusri, A. Achyar, Dwi Hilda Putri, Yusni Atifah, V. Violita","doi":"10.24036/bsc.v7i2.122674","DOIUrl":"https://doi.org/10.24036/bsc.v7i2.122674","url":null,"abstract":". Rice is the main food source for Indonesians. The demand for rice to meet people's needs increases every year due to population growth and efforts to improve nutrition by the community. One of the problems that can affect rice production is drought. Drought is an environmental condition when plants do not get enough water to grow and develop optimally, which can cause a decrease in rice production. To find out how rice growth responds to drought, a study was carried out by giving drought simulation treatments using polyethylene glycol (PEG) on several rice varieties. This study used a completely randomized design that was arranged in a factorial manner with two factors. The first factor was the rice varieties (Harum, Situbagendit, Rosna) the second factor was 0% and 20% PEG concentration. The data obtained were then analyzed statistically using a two-way ANOVA test, and if the results were significantly different, then proceed with Duncan's test at the 5% level.The results showed that the drought simulation treatment had a negative effect on rice growth. Drought simulation treatment using 20% PEG resulted in a decrease in Kadar air relatif (KAR), root length, plant height, and root dry weight of rice. The highest decrease in KAR was found in the sensitive rice variety (Rosna), which was 43.42%. The highest average root length (7.99 cm) was on the sitabaendit variety, and the lowest (5.61) was on the rosna variety. The highest average crown height (17.32 cm) and the lowest (6.61) were on the rosna variety.","PeriodicalId":9003,"journal":{"name":"BioScience","volume":"82 1","pages":""},"PeriodicalIF":10.1,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139308648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Specific primer design and optimization of annealing temperature for amplification gene peroxidase (POD) in Oryza sativa L. 用于扩增红豆杉过氧化物酶（POD）基因的特异性引物设计和退火温度优化

IF 10.1 1区生物学

BioScience Pub Date : 2023-10-31 DOI: 10.24036/bsc.v7i2.122972

Nella Fauziah, A. Achyar, Z. Zulyusri, Yusni Atifah, L. Advinda, V. Violita

引用次数: 0

Antioxidant Activity, Total Phenolic and Flavonoid Content of Honey Bee 蜜蜂的抗氧化活性、总酚和类黄酮含量

IF 10.1 1区生物学

BioScience Pub Date : 2023-10-31 DOI: 10.24036/bsc.v7i2.125200

Narti Fitriana, Risya Raisyah, Siti Nurbayti, Festy Auliaur Rahmah

{"title":"Antioxidant Activity, Total Phenolic and Flavonoid Content of Honey Bee","authors":"Narti Fitriana, Risya Raisyah, Siti Nurbayti, Festy Auliaur Rahmah","doi":"10.24036/bsc.v7i2.125200","DOIUrl":"https://doi.org/10.24036/bsc.v7i2.125200","url":null,"abstract":"Honey is a natural product that has many benefits,for living organism. The composition of honey is very diverse and depends on the type of honey and the region of origin. Bioactive compounds in honey including phenolics and flavonoids cause honey to having antioxidant activity. This study aims to identify honey bees, determine the chemical properties of honey based on the parameters of water content, acidity, and reducing sugar, and analyze the antioxidant activity and phenolic and flavonoid content of honey from Bukit Tiga Puluh National Park Riau and Prabumulih South Sumatra. The research using descriptive method were morphological identification of bees, testing parameters of honey bee chemical properties, DPPH antioxidant test to obtain IC 50 (Inhibition consenteration) values, total phenolic and flavonoid levels. The identified bees are Apis mellifera (from Riau) and A. dorsata (from Prabumulih). This essay showed that the determination of water content, acidity, reducing sugar, both honeys fulfill SNI 8664:2018 except the reducing sugar of A. dorsata honey bee. A. dorsata honey bee has antioxidant activity higher IC 50 value (590 mg/L) than A. mellifera honey (678 mg/L). Total phenolic (TPC) and flavonoid (TFC) of A. mellifera honey were 7.51 mg GAE/g and 0.06 mg QE/g, respectively higher than A. dorsata honey bee (3.35 mg GAE/g and 0.42 mg QE/g).","PeriodicalId":9003,"journal":{"name":"BioScience","volume":"8 1","pages":""},"PeriodicalIF":10.1,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139308014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prediction in ecology and evolution 生态学和进化中的预测

1区生物学

BioScience Pub Date : 2023-10-27 DOI: 10.1093/biosci/biad083

Andrew P Hendry

引用次数: 0

6000 wolves and 331,449,281 people: Can we coexist with other species if we do not abandon anthropocentrism? 6000只狼和331,449,281人:如果我们不放弃人类中心主义，我们能与其他物种共存吗?

1区生物学

BioScience Pub Date : 2023-10-26 DOI: 10.1093/biosci/biad094

Andrea Cardini, Eileen Crist

引用次数: 0

To the stars 对着星星

1区生物学

BioScience Pub Date : 2023-10-26 DOI: 10.1093/biosci/biad095

Scott Glisson

引用次数: 0

The 2023 state of the climate report: Entering uncharted territory 2023年气候状况报告:进入未知领域

1区生物学

BioScience Pub Date : 2023-10-24 DOI: 10.1093/biosci/biad080

William J Ripple, Christopher Wolf, Jillian W Gregg, Johan Rockström, Thomas M Newsome, Beverly E Law, Luiz Marques, Timothy M Lenton, Chi Xu, Saleemul Huq, Leon Simons, Sir David Anthony King

引用次数: 0