Biological Communications最新文献_第4页

The crocodilian forearm and wrist: biomechanics and functional morphology 鳄鱼前臂和手腕:生物力学和功能形态学

Biological Communications Pub Date : 2022-10-10 DOI: 10.21638/spbu03.2022.304

D. Pashchenko

引用次数: 0

Genetic diversity of rhizobial strains isolated from the relict legumes Gueldenstaedtia monophylla and G. verna growing in the republics of Altai and Buryatia (Russia) 阿尔泰共和国和布里亚特共和国（俄罗斯）残留豆科植物Gueldenstaedtia monophylla和G.verna中分离的根瘤菌菌株的遗传多样性

Biological Communications Pub Date : 2022-10-10 DOI: 10.21638/spbu03.2022.301

D. Karlov, A. Sazanova, P. Guro, I. Kuznetsova, A. Verkhozina, A. Belimov, V. Safronova

{"title":"Genetic diversity of rhizobial strains isolated from the relict legumes Gueldenstaedtia monophylla and G. verna growing in the republics of Altai and Buryatia (Russia)","authors":"D. Karlov, A. Sazanova, P. Guro, I. Kuznetsova, A. Verkhozina, A. Belimov, V. Safronova","doi":"10.21638/spbu03.2022.301","DOIUrl":"https://doi.org/10.21638/spbu03.2022.301","url":null,"abstract":"For the first time, bacteria were isolated and identified from the root nodules of relict legumes Gueldenstaedtia monophylla Fisch. and G. verna (Georgi) Boriss. growing in the republics of Altai and Buryatia. The taxonomic position of the 29 obtained isolates was determined by sequencing the 16S rRNA gene (rrs). Showing a significant biodiversity, the isolates from G. monophylla and G. verna belonged to five genera of the order Rhizobiales: Mesorhizobium and Phyllobacterium (family Phyllobacteriaceae), Rhizobium (family Rhizobiaceae), Bosea (family Boseaceae), Bradyrhizobium (family Bradyrhizobiaceae). Three isolates which belonged to the species Bradyrhizobium valentinum and Rhizobium alamii showed 100 % of rrs-similarity with the type strains B. valentinum LmjM3T and R. alamii GBV016T, respectively. Six isolates of the genera Bosea and Rhizobium had a low level of rrs-similarity with the closest type strains (less than 99.5 %), which indicates that they may be assigned to new species. The data obtained can be used to itemise taxonomy within the order Rhizobiales, as well as to reveal the mechanisms of the formation of specific plant-microbial relationships during the evolution of symbiosis by studying the intermediate link between the extinct and modern rhizobia-legume symbiotic systems.","PeriodicalId":8998,"journal":{"name":"Biological Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44029804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation, culturing and 3D bioprinting equine myoblasts 马成肌细胞的分离、培养和3D生物打印

Biological Communications Pub Date : 2022-10-10 DOI: 10.21638/spbu03.2022.302

Alexandr Aimaletdinov, M. Abyzova, I. Kurilov, Alina Yuferova, Catrin Rutland, A. Rizvanov, E. Zakirova

{"title":"Isolation, culturing and 3D bioprinting equine myoblasts","authors":"Alexandr Aimaletdinov, M. Abyzova, I. Kurilov, Alina Yuferova, Catrin Rutland, A. Rizvanov, E. Zakirova","doi":"10.21638/spbu03.2022.302","DOIUrl":"https://doi.org/10.21638/spbu03.2022.302","url":null,"abstract":"Isolating and culturing myoblasts is essential for techniques such as tissue regeneration and in vitro meat production. This research describes a protocol to isolate primary myoblasts from skeletal muscle of an adult horse. The equine primary myoblasts expressed markers specific to myoblasts and had multipotent potential capabilities with differentiation into chondrocytes, adipocytes and osteoblasts in vitro. The horse myoblasts did not adhere to Cytodex 3 and grew poorly on CultiSpher-S microcarriers during in vitro cultivation. Our studies showed that the use of GelMa bioink and ionic cross-linking did not have negative effects on cell proliferation at the beginning of cultivation. However, cells showed reduced proliferative activity by day 40 following in vitro culturing. The population of primary equine myoblasts obtained from an adult individual, and propagated on microcarriers and bioink, did not meet the requirements of the regenerative veterinary and manufacturing meat in vitro regarding the quantity and quality of the cells required. Nonetheless, further optimization of the cell scaling up process, including both microcarriers and/or the bioreactor program and bioprinting, is still important.","PeriodicalId":8998,"journal":{"name":"Biological Communications","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42053597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Preferable and limiting conditions of trans-Palearctic Orthocephalus species (Heteroptera: Miridae) 跨北极原头类物种的优选和限制条件（异翅目：Miridae）

Biological Communications Pub Date : 2022-10-10 DOI: 10.21638/spbu03.2022.305

A. Namyatova

引用次数: 0

Agar-based polyethylene glycol (PEG) infusion model for pea (Pisum sativum L.) — perspectives of translation to legume crop plants 豌豆（Pisum sativum L.）琼脂基聚乙二醇（PEG）灌注模型——转化为豆类作物的前景

Biological Communications Pub Date : 2022-10-10 DOI: 10.21638/spbu03.2022.309

T. Leonova, Julia Shumilina, A. Kim, N. Frolova, L. Wessjohann, T. Bilova, A. Frolov

{"title":"Agar-based polyethylene glycol (PEG) infusion model for pea (Pisum sativum L.) — perspectives of translation to legume crop plants","authors":"T. Leonova, Julia Shumilina, A. Kim, N. Frolova, L. Wessjohann, T. Bilova, A. Frolov","doi":"10.21638/spbu03.2022.309","DOIUrl":"https://doi.org/10.21638/spbu03.2022.309","url":null,"abstract":"Due to the oncoming climate changes water deficit represents one of the most important abiotic stressors which dramatically affects crop productivity worldwide. Because of their importance as the principal source of food protein, legumes attract a special interest of plant scientists. Moreover, legumes are involved in symbiotic association with rhizobial bacteria, which is morphologically localized to root nodules. These structures are critical for fixation of atmospheric nitrogen and highly sensitive to drought. Therefore, new drought-tolerant legume cultivars need to be developed to meet the growing food demand. However, this requires a comprehensive knowledge of the molecular mechanisms behind the plant stress response. To access these mechanisms, adequate and reliable drought stress models need to be established. The agar-based polyethylene glycol (PEG) infusion model allows a physiologically relevant reduction of soil water potential (Ψw), although it is restricted to seedlings and does not give access to proteomics and metabolomics studies. Earlier, we successfully overcame this limitation and optimized this model for mature Arabidopsis plants. Here we make the next step forward and address its application to one of the major crop legumes — pea. Using a broad panel of physiological and biochemical markers, we comprehensively prove the applicability of this setup to legumes. The patterns of drought-related physiological changes are well-interpretable and generally resemble the stress response of plants grown in soil-based stop-watering models. Thus, the proposed model can be efficiently used in the study of stress-related metabolic adjustment in green parts, roots and root nodules of juvenile and flowering plants.","PeriodicalId":8998,"journal":{"name":"Biological Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46531023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Auxin sensitivity improves production of rosmarinic acid in transformed hairy roots of Lavandula angustifolia 生长素敏感性提高狭叶薰衣草毛状根迷迭香酸的产生

Biological Communications Pub Date : 2022-10-10 DOI: 10.21638/spbu03.2022.303

Azade Khadem, A. Bagheri, N. Moshtaghi, Fatemeh Keykha Akhar, A. Sharifi

{"title":"Auxin sensitivity improves production of rosmarinic acid in transformed hairy roots of Lavandula angustifolia","authors":"Azade Khadem, A. Bagheri, N. Moshtaghi, Fatemeh Keykha Akhar, A. Sharifi","doi":"10.21638/spbu03.2022.303","DOIUrl":"https://doi.org/10.21638/spbu03.2022.303","url":null,"abstract":"Hairy root culture is a promising approach to improve production of plant secondary metabolites. The genes, which are located in T-DNA of a root-inducing plasmid, regulate auxin sensitivity of hairy roots. Therefore, this study was aimed to improve the growth and rosmarinic acid production of Lavandula angustifolia hairy roots. Lateral branches of hairy roots were transferred to ½ MS and ½ B5 liquid media. To assess auxin sensitivity, indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) with four different concentrations (0, 0.1, 0.5 and 1 mg/l) were also applied. The growth of hairy roots in ½ MS medium was two-fold higher than in ½ B5 medium. In addition, both auxins were found to significantly improve the growth of hairy roots whereas non-transformed roots stopped growing in the presence of the auxins. The highest dry weight and rosmarinic acid production of hairy roots were obtained from ½ MS medium supplemented with IBA irrespective of its concentration. As a result, the hairy roots grown in ½ MS medium supplemented with IBA produced the maximum amount of rosmarinic acid (7.98 mg/g dry weight of hairy roots). This first report of rosmarinic acid production in L. angustifolia hairy roots provides new insights into the auxin sensitivity of L. angustifolia hairy roots.","PeriodicalId":8998,"journal":{"name":"Biological Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42412198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Changes in heart rate during electrical stimulation of the atrium in rainbow trout (Oncorhynchus mykiss) at low temperature 低温下虹鳟鱼心房电刺激时心率的变化

Biological Communications Pub Date : 2022-06-24 DOI: 10.21638/spbu03.2022.204

N. Kibler, V. Nuzhny, D. Shmakov

{"title":"Changes in heart rate during electrical stimulation of the atrium in rainbow trout (Oncorhynchus mykiss) at low temperature","authors":"N. Kibler, V. Nuzhny, D. Shmakov","doi":"10.21638/spbu03.2022.204","DOIUrl":"https://doi.org/10.21638/spbu03.2022.204","url":null,"abstract":"In this work, we investigated the effect of high heart rate (HR) in vivo on the electrical properties and pumping functions of the heart ventricle of the rainbow trout (Oncorhynchus mykiss) at low ambient temperatures. HR was altered by atrial pacing. The electrocardiogram (ECG) parameters and hemodynamic parameters of the heart ventricle of rainbow trout adapted to a temperature of 5–7 °C were studied from the normal sinus rhythm (21.6 ± 4.9 bpm) to the maximum possible HR. Results show that a HR of about 60 bpm is the upper limit of the normal functional activity of the ventricle of the heart. An increase in heart rate up to 60 bpm leads to an increase in the PQ interval and QRS complex, a decrease in the QT interval on the ECG, and a violation of the rhythmic activity of the heart (i.e., the occurrence of extrasystole), as well as to a considerable change in the hemodynamic parameters of the ventricle of the heart and a decrease in its contractile properties. After a period of ventricular extrasystole for several minutes (10–15 min), the activity of the sinus node resumes but with a lower HR compared with the initial HR. The duration of the QRS complex recovers to the initial one, and the PQ and QT intervals increase. Maximum systolic pressure and end-diastolic pressure also return to their original values after extrasystolic contraction. After the experimental extrasystole, the phenomenon of the absence of an increase in HR is observed. The arising extrasystole probably has a functional nature and is one of the mechanisms of electromechanical homeostatic control in the heart.","PeriodicalId":8998,"journal":{"name":"Biological Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42165045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rate of spontaneous polyploidization in haploid yeast Saccharomyces cerevisiae 单倍体酵母的自发多倍体率

Biological Communications Pub Date : 2022-06-24 DOI: 10.21638/spbu03.2022.202

Yu. V. Andreychuk, A. Zhuk, E. Tarakhovskaya, S. Inge-Vechtomov, E. Stepchenkova

{"title":"Rate of spontaneous polyploidization in haploid yeast Saccharomyces cerevisiae","authors":"Yu. V. Andreychuk, A. Zhuk, E. Tarakhovskaya, S. Inge-Vechtomov, E. Stepchenkova","doi":"10.21638/spbu03.2022.202","DOIUrl":"https://doi.org/10.21638/spbu03.2022.202","url":null,"abstract":"Polyploidization is involved in a variety of biological processes. It is one of the principal mechanisms of new species formation since it provides material for gene diversification and subsequent selection. Multiple cases of polyploidization were registered in different branches of the evolutionary tree of eukaryotes. Besides its role in evolution, polyploidization affects gene expression in living cells: pathological genome duplications often happen in cancer cells. The mechanisms and consequences of polyploidization are being studied extensively. However, quantitative determination of the polyploidization rate is challenging due to its low frequency and the absence of selective genetic markers that would phenotypically distinguish between haploids and polyploids. Our work describes a robust and straightforward method for discriminating haploid and polyploid states in the yeast Saccharomyces cerevisiae, a model organism for studying different aspects of polyploidization. The measurement of polyploidization rate showed that in yeast cells this process is mainly caused by autodiploidization rather than mating-type switching followed by hybridization.","PeriodicalId":8998,"journal":{"name":"Biological Communications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46940109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Lateralization in the visual perception of a potential source of danger in free-ranging steppe marmots (Marmota bobak) 自由放养草原土拨鼠（Marmota bobak）潜在危险源视觉感知的横向化

Biological Communications Pub Date : 2022-06-24 DOI: 10.21638/spbu03.2022.205

I. Demina, A. Dyomin, K. Karenina

引用次数: 0

Lithobiotic cyanobacteria diversity of the Karelian Isthmus 卡累利阿地峡的石生蓝细菌多样性

Biological Communications Pub Date : 2022-06-24 DOI: 10.21638/spbu03.2022.203

O. Rodina, D. Davydov, D. Vlasov

引用次数: 0