American medical journal最新文献

Expression of Exosome-Derived MicroRNAs miR-21 and miR-135 are Differentially Regulated Among Dental Pulp Stem Cells 外泌体衍生的微小RNA miR-21和miR-135在牙髓干细胞中的表达受到差异调节

American medical journal Pub Date : 2023-01-01 DOI: 10.3844/amjsp.2023.1.11

M. Hunsaker, Brandon Richards, Adelle Fuller, K. Kingsley, K. Howard

{"title":"Expression of Exosome-Derived MicroRNAs miR-21 and miR-135 are Differentially Regulated Among Dental Pulp Stem Cells","authors":"M. Hunsaker, Brandon Richards, Adelle Fuller, K. Kingsley, K. Howard","doi":"10.3844/amjsp.2023.1.11","DOIUrl":"https://doi.org/10.3844/amjsp.2023.1.11","url":null,"abstract":": Dental Pulp Stem Cells (DPSCs) are a type of mesenchymal stem cell that has the potential to differentiate into various types of cells and tissue. Previous research has demonstrated that DPSCs can be easily accessed and isolated from both permanent and deciduous teeth, such as wisdom teeth or third molars, although there is less information available about the mechanisms and factors that regulate the growth and proliferative phenotypes and responses of DPSCs. Recent studies have revealed that a type of non-coding RNA known as microRNA can modulate these characteristics among many types of stem cells, although much is still unknown about how DPSC phenotypes may be regulated by microRNAs. Due to this lack of knowledge, the primary objective of this study was to evaluate microRNA expression and determine any correlations with DPSC phenotypes, such as proliferation or growth. Six DPSC isolates were retrieved from an existing repository and cultured using an existing approved protocol. Exosomes and extracellular vesicles were extracted from each DPSC isolate, which ranged in size from 50 to 250 nm. Exosome isolation was confirmed using Western blots for CD63 and Bradford protein assays. In addition, RNA was extracted, cDNA was synthesized, and qPCR was performed, which revealed that all DPSC isolates expressed miR-124, miR-133, and miR-224. However, differential expression of miR-21 among rapid and intermediate doubling time (rDT, iDT) isolates was observed, with expression of miR-135 found only among the intermediate and slow (iDT, sDT) DPSC isolates. This study provides some of the first evidence of associations between miRNA expression and specific DPSC growth phenotypes. Further studies will be needed to confirm these results and determine the mechanisms associated with the expression of miR-21 among rapidly growing DPSCs and miR-135 expression among more slowly growing DPSCs","PeriodicalId":89887,"journal":{"name":"American medical journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41896728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transfusion-Related Acute Lung Injury 输血相关性急性肺损伤

American medical journal Pub Date : 2020-01-01 DOI: 10.3844/amjsp.2020.1.4

F. Raziq

引用次数: 0

Effects of Vascular Endothelial Growth Factor (VEGF) on Dental Pulp Stem Cells (DPSC) 血管内皮生长因子（VEGF）对牙髓干细胞（DPSC）的影响

American medical journal Pub Date : 2019-01-01 DOI: 10.3844/AMJSP.2019.1.8

E. Mullins, Cale Forgues, K. Kingsley

{"title":"Effects of Vascular Endothelial Growth Factor (VEGF) on Dental Pulp Stem Cells (DPSC)","authors":"E. Mullins, Cale Forgues, K. Kingsley","doi":"10.3844/AMJSP.2019.1.8","DOIUrl":"https://doi.org/10.3844/AMJSP.2019.1.8","url":null,"abstract":"Dental Pulp Stem Cells (DPSCs) are non-embryonic, mesenchymal stem cells that may have significant potential for therapeutic and regenerative biomedical applications. Studies of DPSC differentiation have demonstrated the potential to form many tissue types, including neural, osteogenic and vascular precursors using cytokines and growth factors, such as Vascular Endothelial Growth Factor (VEGF). Eight previously isolated Dental Pulp Stem Cell (DPSC) isolates were grown in culture and treated with VEGF to evaluate any effects on growth, viability or biomarker expression. Administration of VEGF at 10 ng/mL significantly inhibited growth in two rapidly dividing or rDT DPSC isolates, with no other measurable effects noted among the intermediate (iDT) or slow (sDT) growing DPSC isolates. In addition, administration of VEGF had no significant effects on viability of the sDT or iDT DPSC isolates, however, all three of the rapidly dividing or rDT DPSC isolates exhibited significantly increased viability. Finally, mRNA expression of osteogenic biomarkers Alkaline Phosphatase (ALP) and Dentin Sialophosphoprotein (DSPP) was observed among the rDT isolates with specific combinations of DPSC biomarkers expressed (NANOG in combination with Sox-2 or Oct-4 but not both). The results of these data suggest that VEGF administration may be sufficient to induce partial differentiation of DPSC isolates, although this may be dependent upon the MSC biomarker expression of the DPSCs. These preliminary data may further research into the potential for tissue regeneration and bioengineering.","PeriodicalId":89887,"journal":{"name":"American medical journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3844/AMJSP.2019.1.8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48487429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Primary Eosinophilic Lung Diseases and the Therapeutic Role of Corticosteroids: A Case Report and Literature Review 原发性嗜酸性肺疾病和皮质类固醇的治疗作用:1例报告和文献复习

American medical journal Pub Date : 2019-01-01 DOI: 10.3844/amjsp.2019.9.13

H. Sani, N. S. Zulkufli

引用次数: 0

Is Air Pollution Controlled Enough? “Tapered Probabilities” Answer 空气污染控制得足够吗？“递减概率”答案

American medical journal Pub Date : 2017-11-06 DOI: 10.3844/AMJSP.2017.14.28

R. Shanmugam

引用次数: 0

Level of Non-Matching Primary Transplant Cases According to Repeat Transplants Cases and a New Bivariate Poisson Distribution 根据重复移植病例和新的二元泊松分布的不匹配初次移植病例水平

American medical journal Pub Date : 2017-07-10 DOI: 10.3844/AMJSP.2017.1.13

R. Shanmugam

{"title":"Level of Non-Matching Primary Transplant Cases According to Repeat Transplants Cases and a New Bivariate Poisson Distribution","authors":"R. Shanmugam","doi":"10.3844/AMJSP.2017.1.13","DOIUrl":"https://doi.org/10.3844/AMJSP.2017.1.13","url":null,"abstract":"In their practice, healthcare administrators and professionals often wonder about the non-matching level organs in transplants for the sake of future forecasting. Currently, there is no appropriate methodology to analyze the pertinent transplant data and describe the patterns. The lack of a suitable methodology in the literature originates from an incorrect impression that the primary transplant cases and the repeat transplant cases are two separate and independent Poisson probability processes. In fact, the actual data on the primary and repeat transplant cases in USA during the year 2014 indicate otherwise with a high degree of correlation between them. One wonders about the missing link and it hides in their model as this article articulates. The aims of this article are set to find an appropriate underlying model for the data and then construct an analytic methodology. In this research process, a novel and useful bivariate probability distribution is discovered and it is named here \"seemingly independent bivariate Poisson distribution\" for a lack of better title. Its statistical properties are derived, explained and illustrated. This new bivariate distribution helps not only to estimate the non-matching level of organs in the transplant cases but also to project the number of repeat transplant cases based on knowing the number of primary transplant cases and vice versa.","PeriodicalId":89887,"journal":{"name":"American medical journal","volume":"8 1","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"2017-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3844/AMJSP.2017.1.13","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43966138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Self Medication with Antibiotics among Medical and Pharmacy Students in North India 印度北部医学和药学学生的抗生素自我用药

American medical journal Pub Date : 2016-12-30 DOI: 10.3844/AMJSP.2016.7.12

B. Pal, K. Murti, A. Gupta, Urmi Choudhury, M. K. Rastogi, H. Pandey, C. Lal, K. Pandey, P. Das

{"title":"Self Medication with Antibiotics among Medical and Pharmacy Students in North India","authors":"B. Pal, K. Murti, A. Gupta, Urmi Choudhury, M. K. Rastogi, H. Pandey, C. Lal, K. Pandey, P. Das","doi":"10.3844/AMJSP.2016.7.12","DOIUrl":"https://doi.org/10.3844/AMJSP.2016.7.12","url":null,"abstract":"Self medication practices among the medical and paramedical professionals have been reported in many countries, however, very limited research was done exclusively on antibiotic in North India. This study was designed to assess the prevalence and pattern of antibiotic usage for self diagnosed diseases among the medical and pharmacy students. This was a questionnaire based cross sectional study conducted among medical and pharmacy students in North India. A total 326 students participated in the study and data of 316 students were eligible for analysis. 57.63% medical and 66.9% pharmacy students reported to use antibiotics in last one year. Fever was the predominant ailments for which medical (48.41%) and pharmacy (46.88%) students' self medicated. Most of the medical (74.6%) and pharmacy (61.9%) students had preferred amoxicillin for their ailments. The majority of medical students, 65.87% have self medicated because of their pharmacological knowledge in contrast to 62.1% pharmacy students. Adverse drug reaction experienced by 29.36% and 11.7% medical and pharmacy students respectively. Statistically no significant difference was observed in antibiotic usage between medical and pharmacy students (p = 0.08). To curb the growing trend of this unethical practices students of health care professional needs to be educated.","PeriodicalId":89887,"journal":{"name":"American medical journal","volume":"7 1","pages":"7-12"},"PeriodicalIF":0.0,"publicationDate":"2016-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3844/AMJSP.2016.7.12","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70193124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

A Case Vignette and Review of Maladaptive Denial and Related Ethical Issues 适应不良否认及相关伦理问题的个案回顾

American medical journal Pub Date : 2016-11-12 DOI: 10.3844/amjsp.2016.3.6

M. Prevost, Wendy Gerstein, N. Salas, C. Geppert, Holly Fleming

引用次数: 0

Naloxone as a Harm Reduction Initiative: Misinterpretations of Research 纳洛酮作为减少危害的倡议:对研究的误解

American medical journal Pub Date : 2016-04-09 DOI: 10.3844/AMJSP.2016.1.2

Richard R. Massatti

引用次数: 1

What is Missing in STD Screening in Hong Kong 香港性传播疾病筛检缺少什么

American medical journal Pub Date : 2016-02-01 DOI: 10.3844/AMJSP.2016.13.18

C. Chan, W. Chan, C. H. Cheng, P. Xia

{"title":"What is Missing in STD Screening in Hong Kong","authors":"C. Chan, W. Chan, C. H. Cheng, P. Xia","doi":"10.3844/AMJSP.2016.13.18","DOIUrl":"https://doi.org/10.3844/AMJSP.2016.13.18","url":null,"abstract":"This retrospective analysis was to study the prevalence of Sexually Transmitted Diseases (STD) in a Reproductive Medical Center in Hong Kong. A total of 1190 patients were included in this study. Group 1A, couples had no symptoms but presented with subinfertility; Group 1B, the subfertile couples were positive for either Chlamydia trachomatis (CT) or Ureaplasma urealyticum (UU); Group 2, couples with symptoms were offered full STD screening including CT, UU, Mycoplasma hominis (MH), Neisseria gonorrhoea (NG), Syphilis, Herpes Simplex Virus 1 and 2. The methods of ELISA and quantitative real-time PCR were used for these analyses. Group 1A: UU detection rates in both male and female (13.84 v.s. 37.07%) were significantly higher than that of CT (5.65 v.s. 5.57%); Group 1B and Group 2: For those who had Full STD check, UU and MH detection rates were significantly higher than that of CT (35, 13.7 and 7.1% respectively). Over 47% of patients showed positive for one or more organism. In the subfertile couples, the UU and CT detection rates were much higher in females than those in males. Both semen and urine samples gave the same rates of infection among CT, UU, NG and MH. U. urealyticum infection rate rather than Chlamydis trachomatis infection is highest in Hong Kong. The infection rate in females is higher than in males. The detection rates in semen and urine samples in males are similar.","PeriodicalId":89887,"journal":{"name":"American medical journal","volume":"7 1","pages":"13-18"},"PeriodicalIF":0.0,"publicationDate":"2016-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3844/AMJSP.2016.13.18","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70193058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1