Results in immunology最新文献_第8页

Snapshot of spatio-temporal cytokine responses to single and co-infections with helminths and bacteria 对寄生虫和细菌单独感染和联合感染的时空细胞因子反应的快照

Results in immunology Pub Date : 2011-05-01 Epub Date: 2011-11-04 DOI: 10.1016/j.rinim.2011.10.001

Ashutosh K. Pathak , Michael C. Biarnes , Lisa Murphy , Isabella M. Cattadori

{"title":"Snapshot of spatio-temporal cytokine responses to single and co-infections with helminths and bacteria","authors":"Ashutosh K. Pathak , Michael C. Biarnes , Lisa Murphy , Isabella M. Cattadori","doi":"10.1016/j.rinim.2011.10.001","DOIUrl":"10.1016/j.rinim.2011.10.001","url":null,"abstract":"<div>Cytokines play a key role in maintaining communication between organs and in so doing modulate the interaction between concurrent infections. The extent of these effects depends on the properties of the organ infected and the intensity and type of infections. To determine systemic bystander effects among organs, IFN-γ, IL-4 and IL-10 gene expression was quantified at 7 days post-challenge in directly infected and uninfected organs during single and co-infections with the respiratory bacterium Bordetella bronchiseptica and the gastrointestinal helminths Graphidium strigosum and Trichostrongylus retortaeformis. Results showed that cytokine expression in a specific organ was influenced by the type of infection occurring in another organ, and this bystander effect was more apparent in some organs than others. Within the same organ the relative cytokine expression was consistent across infections, although some cytokines were more affected by bystander effects than others. For the infected gastrointestinal tract, a stronger cytokine response was observed in the tissue that harbored the majority of helminths (i.e. duodenum and fundus). Overall, co-infections altered the intensity but to a lesser extent the relative cytokine profile against the focal infection, indicating clear bystander effects and low organ compartmentalization. However, organs appear to actively modulate cytokine expression to avoid potential immuno-pathological consequences.</div>","PeriodicalId":89845,"journal":{"name":"Results in immunology","volume":"1 1","pages":"Pages 95-102"},"PeriodicalIF":0.0,"publicationDate":"2011-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.rinim.2011.10.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31983468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Hematopoietic cell populations in dolphin bone marrow: Analysis of colony formation and differentiation 海豚骨髓中的造血细胞群:集落形成和分化的分析

Results in immunology Pub Date : 2011-05-01 Epub Date: 2011-05-19 DOI: 10.1016/j.rinim.2011.05.004

Takao Segawa , Takuya Itou , Miwa Suzuki , Tadaaki Moritomo , Teruyuki Nakanishi , Takeo Sakai

{"title":"Hematopoietic cell populations in dolphin bone marrow: Analysis of colony formation and differentiation","authors":"Takao Segawa , Takuya Itou , Miwa Suzuki , Tadaaki Moritomo , Teruyuki Nakanishi , Takeo Sakai","doi":"10.1016/j.rinim.2011.05.004","DOIUrl":"10.1016/j.rinim.2011.05.004","url":null,"abstract":"<div>Bone marrow biopsy is useful for diagnosis of hematopoietic diseases. We have recently reported that bone marrow biopsy from the flipper might be useful for diagnosis of hematopoietic diseases in dolphins. In this study, to demonstrate whether biopsy from the flipper is useful for clinical diagnosis, we investigated the gene expression profiles and proliferation and differentiation of bone marrow mononuclear cell (BMMC) isolated from the humeral bone marrow of bottlenose dolphins. BMMC exhibited gene expression profiles considered to be characteristic of hematopoietic cells. Similarly, a colony forming unit assay showed that dolphin BMMC possessed vigorous colony forming ability. The proliferation of hematopoietic progenitor cells resulted in the formation of three types of colonies, containing neutrophils, monocytes/macrophages and eosinophils with or without megakaryocytes, all of which could be identified based on the morphological characteristics and gene expression profiles typically associated with hematopoietic markers. Thus, dolphin BMMCs from humeral bone marrow contain many hematopoietic progenitor cells, and bone marrow biopsy from the flipper is suggested useful for clinical diagnosis for the dolphins.</div>","PeriodicalId":89845,"journal":{"name":"Results in immunology","volume":"1 1","pages":"Pages 1-5"},"PeriodicalIF":0.0,"publicationDate":"2011-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.rinim.2011.05.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31983075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Efficient expansion of cryopreserved CD4+CD25+CD127lo/− cells in Type 1 diabetes 低温保存的CD4+CD25+CD127lo/−细胞在1型糖尿病中的高效扩增

Results in immunology Pub Date : 2011-05-01 Epub Date: 2011-08-22 DOI: 10.1016/j.rinim.2011.08.001

Anna Rydén , Maria Faresjö

引用次数: 10

Molecular cloning and characterisation of the rock bream, Oplegnathus fasciatus, Fas (CD95/APO-1), and its expression analysis in response to bacterial or viral infection 板鱼Fas (CD95/APO-1)的克隆、鉴定及其对细菌和病毒感染的表达分析

Results in immunology Pub Date : 2011-05-01 Epub Date: 2011-07-12 DOI: 10.1016/j.rinim.2011.06.002

Ji-Min Jeong , Ju-Won Kim , Hyoung-Jun Park , Jeong-Hun Song , Do-Hyung Kim , Chan-Il Park

{"title":"Molecular cloning and characterisation of the rock bream, Oplegnathus fasciatus, Fas (CD95/APO-1), and its expression analysis in response to bacterial or viral infection","authors":"Ji-Min Jeong , Ju-Won Kim , Hyoung-Jun Park , Jeong-Hun Song , Do-Hyung Kim , Chan-Il Park","doi":"10.1016/j.rinim.2011.06.002","DOIUrl":"10.1016/j.rinim.2011.06.002","url":null,"abstract":"<div>Fas belongs to the tumour necrosis factor (TNF) receptor superfamily and can transmit a death signal leading to apoptosis. In the present study, we isolated the full-length cDNA for rock bream (Oplegnathus fasciatus) Fas (RbFas). The full-length RbFas cDNA was 1770bp long and contained an open reading frame of 957bp that encoded 319 amino acid residues with a predicted molecular mass of 35.1kDa. The 319 amino-acid predicted RbFas sequence is homologous to other Fas sequences, contains three cysteine-rich domains and a death domain (DD) and two potential N-glycosylation sites. Expression of RbFas mRNA was detected in nine different tissues from healthy rock bream and was the highest in red blood cells. In analyses of mitogen-stimulated RbFas expression in peripheral blood leucocytes, expression of RbFas mRNA was observed between 1 and 36h after stimulation with LPS, and 1 and 3h stimulation with poly I:C. In the case of bacterial injection, the RbFas transcript peaked 6h after injection in both the kidney and the spleen. Otherwise, the RbFas transcript peaked after 1h in spleen and 6h in kidney following injection with RSIV.</div>","PeriodicalId":89845,"journal":{"name":"Results in immunology","volume":"1 1","pages":"Pages 11-17"},"PeriodicalIF":0.0,"publicationDate":"2011-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.rinim.2011.06.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31983077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Peritoneal macrophages from patients with cirrhotic ascites show impaired phagocytosis and vigorous respiratory burst 肝硬化腹水患者的腹膜巨噬细胞表现为吞噬功能受损和剧烈的呼吸爆发

Results in immunology Pub Date : 2011-05-01 Epub Date: 2011-09-01 DOI: 10.1016/j.rinim.2011.08.004

Abdel Motaal M. Ahmed , Adrian Bomford , Kayhan T. Nouri-Aria , Ted Davies , Roger Smith , Roger Williams

引用次数: 10

Advisory Board 咨询委员会

Results in immunology Pub Date : 2011-05-01 Epub Date: 2011-12-26 DOI: 10.1016/S2211-2839(11)00020-7

引用次数: 0

Localization of antimicrobial peptides in the tunic of Ciona intestinalis (Ascidiacea, Tunicata) and their involvement in local inflammatory-like reactions 海鞘鞘(Ascidiacea, Tunicata)中抗菌肽的定位及其参与局部炎症样反应

Results in immunology Pub Date : 2011-05-01 Epub Date: 2011-09-21 DOI: 10.1016/j.rinim.2011.09.001

M.A. Di Bella , H. Fedders , G. De Leo , M. Leippe

{"title":"Localization of antimicrobial peptides in the tunic of Ciona intestinalis (Ascidiacea, Tunicata) and their involvement in local inflammatory-like reactions","authors":"M.A. Di Bella , H. Fedders , G. De Leo , M. Leippe","doi":"10.1016/j.rinim.2011.09.001","DOIUrl":"10.1016/j.rinim.2011.09.001","url":null,"abstract":"<div>Tunicates comprising a wide variety of different species synthesize antimicrobial peptides as important effector molecules of the innate immune system. Recently, two putative gene families coding for antimicrobial peptides were identified in the expressed sequence tag database of the tunicate Ciona intestinalis. Two synthetic peptides representing the cationic core region of one member of each of the families displayed potent antibacterial and antifungal activities. Moreover, the natural peptides were demonstrated to be synthesized and stored in distinct hemocyte types. Here, we investigated the presence of these natural peptides, namely Ci-MAM-A and Ci-PAP-A, in the tunic of C. intestinalis considering that the ascidian tunic is a body surface barrier exposed to constant microbial assault. Furthermore, as the tunic may represent a major route of entry for pathogen invasion after its damage we monitored the location of these peptides upon a local inflammatory-like reaction induced by injection of foreign cells. Using immunocytochemistry and electron microscopy both peptides were localized to the tunic and were massively present in granulocytes of inflamed tissue. Conclusively, antimicrobial peptides may constitute a chemical barrier within the tunic of urochordates.</div>","PeriodicalId":89845,"journal":{"name":"Results in immunology","volume":"1 1","pages":"Pages 70-75"},"PeriodicalIF":0.0,"publicationDate":"2011-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.rinim.2011.09.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31983464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Molecular modeling and expression of the Litopenaeus vannamei proliferating cell nuclear antigen (PCNA) after white spot syndrome virus shrimp infection 虾感染白斑综合征病毒后凡纳滨对虾增殖细胞核抗原(PCNA)的分子模拟与表达

Results in immunology Pub Date : 2011-05-01 Epub Date: 2011-07-26 DOI: 10.1016/j.rinim.2011.07.001

Enrique de-la-Re-Vega , Adriana Muhlia-Almazan , Aldo A. Arvizu-Flores , Maria A. Islas-Osuna , Gloria Yepiz-Plascencia , Luis G. Brieba , Rogerio R. Sotelo-Mundo

{"title":"Molecular modeling and expression of the Litopenaeus vannamei proliferating cell nuclear antigen (PCNA) after white spot syndrome virus shrimp infection","authors":"Enrique de-la-Re-Vega , Adriana Muhlia-Almazan , Aldo A. Arvizu-Flores , Maria A. Islas-Osuna , Gloria Yepiz-Plascencia , Luis G. Brieba , Rogerio R. Sotelo-Mundo","doi":"10.1016/j.rinim.2011.07.001","DOIUrl":"10.1016/j.rinim.2011.07.001","url":null,"abstract":"<div>Proliferating cell nuclear antigen (PCNA) is the eukaryotic sliding clamp that tethers DNA polymerase to DNA during replication. The full-length cDNA of the Pacific white shrimp Litopenaeus vannamei PCNA (LvPCNA) was cloned and encoded a protein of 260 amino acids that is highly similar to other Crustacean PCNAs. The theoretical shrimp PCNA structure has all the domains that are necessary for its interaction with template DNA and DNA polymerase. RT-PCR analysis showed that LvPCNA is expressed mainly in muscle and hemocytes and much less in hepatopancreas and gills. LvPCNA mRNA levels are not statistically different in muscle from healthy and challenged shrimp with the white spot syndrome virus (WSSV). In contrast, the mRNA levels of the viral DNA polymerase show a biphasic pattern with expression at 6h post-infection and later at 24 and 48h. These results suggest that in shrimp muscle LvPCNA levels are steadily kept to allow viral replication and that WSSV DNA polymerase (WSSV-DNApol) is more responsive towards later stages of infection. More knowledge of the DNA replication machinery would result in a better understanding of the mechanism and components of viral replication, since the WSSV genome does not have all the components required for assembly of a fully functional replisome.</div>","PeriodicalId":89845,"journal":{"name":"Results in immunology","volume":"1 1","pages":"Pages 24-30"},"PeriodicalIF":0.0,"publicationDate":"2011-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.rinim.2011.07.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31983079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Molecular characterization and phylogenetic analysis of two antimicrobial peptides: Anti-lipopolysaccharide factor and crustin from the brown mud crab, Scylla serrata 褐泥蟹抗脂多糖因子和壳蛋白两种抗菌肽的分子特征及系统发育分析

Results in immunology Pub Date : 2011-05-01 Epub Date: 2011-07-08 DOI: 10.1016/j.rinim.2011.06.001

V.V. Afsal, Swapna P. Antony, Naveen Sathyan, Rosamma Philip

{"title":"Molecular characterization and phylogenetic analysis of two antimicrobial peptides: Anti-lipopolysaccharide factor and crustin from the brown mud crab, Scylla serrata","authors":"V.V. Afsal, Swapna P. Antony, Naveen Sathyan, Rosamma Philip","doi":"10.1016/j.rinim.2011.06.001","DOIUrl":"10.1016/j.rinim.2011.06.001","url":null,"abstract":"<div>AMPs are evolutional weapons, widely used by animals and plants in their innate immune system to fend off invading microbes. The present study reports characterization of a new ALF isoform (Sc-ALF; HQ638024) and the first crustin (Sc-crustin; HQ638025) from the mud crab, Scylla serrata. The full-length cDNA of Sc-ALF consisted of 477bp with an ORF of 123 amino acids and a putative signal peptide of 26 amino acids. Sc-ALF had a predicted molecular weight (MW) of 11.17kDa and theoretical isoelectric point (pI) of 9.95. Two highly conserved cysteine residues and putative LPS binding domain were observed in Sc-ALF. Comparison of amino acid sequences with neighbor-joining tree indicated that Sc-ALF shared maximum similarity with ALF of S. paramamosain. Peptide model of Sc-ALF created using SWISS-MODEL server was found to consist of two α-helices crowded against a four-strand β-sheet. The full-length cDNA of Sc-crustin consisted of 433 base pairs with an ORF of 111 amino acids and a putative signal peptide of 21 amino acids. Comparison of amino acid sequences with a neighbor-joining tree revealed that Sc-crustin shared high identity with other known crustins characterized from S. paramamosain, P. trituberculatus, H. araneus, C. maenas and F. chinensis. A whey-acidic-protein domain could be detected at the C-terminus with the characteristic four disulfide core. Sc-crustin had a predicted MW of 10.24kDa and a pI of 8.76. Peptide model of Sc-crustin created using SWISS-MODEL server indicated a random coiled structure that is with two possible β-sheets but no helices.</div>","PeriodicalId":89845,"journal":{"name":"Results in immunology","volume":"1 1","pages":"Pages 6-10"},"PeriodicalIF":0.0,"publicationDate":"2011-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.rinim.2011.06.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31983076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

CD3γ/δ in sea bass (Dicentrarchus labrax): Molecular characterization and expression analysis 黑鲈(Dicentrarchus labrax) CD3γ/δ的分子特征及表达分析

Results in immunology Pub Date : 2011-05-01 Epub Date: 2011-08-30 DOI: 10.1016/j.rinim.2011.08.003

Elisa Randelli, Giuseppe Scapigliati, Francesco Buonocore

{"title":"CD3γ/δ in sea bass (Dicentrarchus labrax): Molecular characterization and expression analysis","authors":"Elisa Randelli, Giuseppe Scapigliati, Francesco Buonocore","doi":"10.1016/j.rinim.2011.08.003","DOIUrl":"10.1016/j.rinim.2011.08.003","url":null,"abstract":"<div>The CD3 complex is the common marker on the surface of both αβ and γδ T cells and is essential for formation of the T-cell receptor complex and for T-cell activation.In this paper, we report the gene cloning and molecular characterization of a CD3γ/δ homologue in sea bass (Dicentrarchus labrax), the analysis of transcription levels in lymphoid and non-lymphoid organs and the gene regulation after in vitro stimulation with LPS and PHA.Four cysteine residues in the extracellular domain, involved in the constitution of immunoglobulin-like domain, are present in sea bass CD3γ/δ sequence and they are conserved both in number and position from mammals to teleost sequences. Similar to other known CD3γ/δs, in sea bass CD3γ/δ there is also a conserved immunoreceptor tyrosine-based activation ITAM motif that could be responsible for its individual signal transduction capacity.The real time RT-PCR basal analysis shows the highest level of CD3γ/δ mRNA in thymus, followed by peripheral blood leucocytes, spleen, gills, gut, liver, head kidney, brain and muscle. The expression analysis under stimuli condition reveals a significant decrease of CD3γ/δ expression after LPS stimulation and a significant increase after PHA-L stimulation, in agreement with mammals results.In conclusion, these data allow us to affirm that sea bass CD3γ/δ can be used as a T cell marker and will help in adding new insight on the immune response mechanisms of sea bass.</div>","PeriodicalId":89845,"journal":{"name":"Results in immunology","volume":"1 1","pages":"Pages 31-35"},"PeriodicalIF":0.0,"publicationDate":"2011-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.rinim.2011.08.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31983080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9