Efficient expansion of cryopreserved CD4+CD25+CD127lo/− cells in Type 1 diabetes

Results in immunology Pub Date : 2011-05-01 Epub Date: 2011-08-22 DOI:10.1016/j.rinim.2011.08.001

Anna Rydén , Maria Faresjö

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引用次数: 10

Abstract

Increased attention has been drawn to the important role played by regulatory T-cells (Treg) in immune homoeostasis. However, the small numbers of Tregs make them elusive to study. We investigated the cryostability of Tregs and whether they can be expanded from cryopreserved peripheral blood mononuclear cells (PBMCs). Further, to elucidate if there is a difference in ex-vivo frequency or in vitro expansion of Tregs among T1D children (n=9), high-risk (n=7) and healthy (n=10) individuals, Tregs defined as CD4⁺CD25⁺CD127^lo/− were isolated from cryopreserved PBMCs.

Cryopreserved PBMCs maintained a stable expression of Treg-markers. Tregs were efficiently expanded in vitro from all donors and Tregs from T1D children acquired higher FOXP3 expression compared to healthy subjects. T1D children had a significantly lower percentage of Tregs among CD4⁺ T-cells and also lower Treg to CD4⁺CD25⁻ cell ratios compared to healthy individuals.

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低温保存的CD4+CD25+CD127lo/−细胞在1型糖尿病中的高效扩增

调节性t细胞(Treg)在免疫平衡中的重要作用已引起越来越多的关注。然而，treg的数量很少，使它们难以研究。我们研究了Tregs的低温稳定性，以及它们是否可以从冷冻保存的外周血单个核细胞(PBMCs)中扩增。此外，为了阐明在T1D儿童(n=9)、高危人群(n=7)和健康人群(n=10)中，treg的离体频率或体外扩增是否存在差异，我们从冷冻保存的PBMCs中分离出定义为CD4+CD25+CD127lo/−的treg。冷冻保存的pbmc保持treg标记物的稳定表达。所有供体的treg均能在体外有效扩增，与健康受试者相比，T1D儿童的treg具有更高的FOXP3表达。与健康个体相比，T1D儿童CD4+ t细胞中Treg的百分比明显较低，Treg与CD4+CD25 -细胞的比率也较低。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Results in immunology

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