Peritoneal macrophages from patients with cirrhotic ascites show impaired phagocytosis and vigorous respiratory burst

Results in immunology Pub Date : 2011-05-01 Epub Date: 2011-09-01 DOI:10.1016/j.rinim.2011.08.004

Abdel Motaal M. Ahmed , Adrian Bomford , Kayhan T. Nouri-Aria , Ted Davies , Roger Smith , Roger Williams

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引用次数: 10

Abstract

Cirrhotic patients (CPs) are susceptible to spontaneous bacterial peritonitis (SBP). Aim of this study was to examine if this susceptibility was related to peritoneal macrophages' (PMs) altered host defence. Absorbance of phagocytosed particles by PMs from CPs was lower than that of control (31.88% vs. 77.2%). Particle opsonisation increased the absorbance to 41% in CPs' PMs, and this value remains lower than the control; 77.2%. Respiratory burst (RB) was expressed as fluorescence index values, and these were higher in PMs from CPs than in controls (82 vs. 41, 73 vs. 26 and 71 vs. 26). IFN-γ made no further increase of RB values in PMs from CPs. CD14 expression was also higher in CPs' PMs. IFN-γ significantly downregulated CD14 expression in both CPs' PMs and control. Reduced phagocytosis by predominantly CD14-positive PMs from CPs could be related to intense RB. Findings suggest altered host defence that could contribute to susceptibility to SBP.

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肝硬化腹水患者的腹膜巨噬细胞表现为吞噬功能受损和剧烈的呼吸爆发

肝硬化患者易发生自发性细菌性腹膜炎(SBP)。本研究的目的是检查这种易感性是否与腹膜巨噬细胞(pm)改变的宿主防御有关。PMs对CPs吞噬颗粒的吸光度低于对照(31.88%比77.2%)。粒子共振使CPs的吸光度增加到41%，这个值仍然低于对照组;77.2%。呼吸爆发(RB)以荧光指数表示，与对照组相比，CPs患者的呼吸爆发指数更高(82 vs. 41, 73 vs. 26, 71 vs. 26)。IFN-γ没有进一步增加cp对pm的RB值。CD14在CPs的pm中的表达也更高。IFN-γ在CPs的pm和对照组中均显著下调CD14的表达。主要来自CPs的cd14阳性pm的吞噬减少可能与强烈的RB有关。研究结果表明，宿主防御的改变可能导致对SBP的易感性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Results in immunology

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