Results in immunology最新文献_第3页

DNA-encapsulated magnesium phosphate nanoparticles elicit both humoral and cellular immune responses in mice dna包裹的磷酸镁纳米颗粒在小鼠体内引起体液和细胞免疫反应

Results in immunology Pub Date : 2014-01-01 DOI: 10.1016/j.rinim.2014.04.001

Gajadhar Bhakta , Victor Nurcombe , Amarnath Maitra , Anju Shrivastava

{"title":"DNA-encapsulated magnesium phosphate nanoparticles elicit both humoral and cellular immune responses in mice","authors":"Gajadhar Bhakta , Victor Nurcombe , Amarnath Maitra , Anju Shrivastava","doi":"10.1016/j.rinim.2014.04.001","DOIUrl":"10.1016/j.rinim.2014.04.001","url":null,"abstract":"<div>The efficacy of pEGFP (plasmid expressing enhanced green fluorescent protein)-encapsulated PEGylated (meaning polyethylene glycol coated) magnesium phosphate nanoparticles (referred to as MgPi-pEGFP nanoparticles) for the induction of immune responses was investigated in a mouse model. MgPi-pEGFP nanoparticles induced enhanced serum antibody and antigen-specific T-lymphocyte responses, as well as increased IFN-γ and IL-12 levels compared to naked pEGFP when administered via intravenous, intraperitoneal or intramuscular routes. A significant macrophage response, both in size and activity, was also observed when mice were immunized with the nanoparticle formulation. The response was highly specific for the antigen, as the increase in interaction between macrophages and lymphocytes as well as lymphocyte proliferation took place only when they were re-stimulated with recombinant green fluorescence protein (rGFP). Thus the nanoparticle formulation elicited both humoral as well as cellular responses. Cytokine profiling revealed the induction of Th-1 type responses. The results suggest DNA-encapsulated magnesium phosphate (MgPi) nanoparticles may constitute a safer, more stable and cost-efficient DNA vaccine formulation.</div>","PeriodicalId":89845,"journal":{"name":"Results in immunology","volume":"4 ","pages":"Pages 46-53"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.rinim.2014.04.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32429947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

IL-6 signalling in patients with acute ST-elevation myocardial infarction 急性st段抬高型心肌梗死患者的IL-6信号传导

Results in immunology Pub Date : 2014-01-01 DOI: 10.1016/j.rinim.2013.11.002

Vibeke N. Ritschel , Ingebjørg Seljeflot , Harald Arnesen , Sigrun Halvorsen , Thomas Weiss , Jan Eritsland , Geir Ø Andersen

{"title":"IL-6 signalling in patients with acute ST-elevation myocardial infarction","authors":"Vibeke N. Ritschel , Ingebjørg Seljeflot , Harald Arnesen , Sigrun Halvorsen , Thomas Weiss , Jan Eritsland , Geir Ø Andersen","doi":"10.1016/j.rinim.2013.11.002","DOIUrl":"10.1016/j.rinim.2013.11.002","url":null,"abstract":"<div>Cytokines of the IL-6 family have been related to infarct size and prognosis in patients with myocardial infarction. The aims of the present study were to elucidate possible associations between myocardial necrosis and left ventricular impairment and members of the IL-6 transsignalling system including soluble (s) IL-6R and (s) glycoprotein 130 (sgp130) in patients with ST-elevation myocardial infarction (STEMI) treated with primary PCI.In blood samples from 1028 STEMI patients, collected in-hosptial, we found significant correlations between peak TnT and IL-6 and CRP (p < 0.001, all) and between IL-6 and CRP and LV ejection fraction and NT-proBNP (p < 0.001, all). On the contrary, no significant associations were found between peak TnT and sgp130 or sIL-6R. Furthermore sgp130 was significantly elevated in diabetic patients and also associated with the glucometabolic state.In conclusion, circulating levels of IL-6 and CRP, but not the soluble forms of the receptor (sIL-6R) or the receptor signalling subunit (sgp130) were associated with the extent of myocardial necrosis. The biological importance of the IL-6/gp130-mediated signalling pathways in patients with acute myocardial infarction and dysglycemia should be further elucidated.</div>","PeriodicalId":89845,"journal":{"name":"Results in immunology","volume":"4 ","pages":"Pages 8-13"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.rinim.2013.11.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32241091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 60

Establishment of c-myc-immortalized Kupffer cell line from a C57BL/6 mouse strain C57BL/6小鼠株c-myc永生化库普弗细胞系的建立

Results in immunology Pub Date : 2014-01-01 DOI: 10.1016/j.rinim.2014.08.001

Hiroshi Kitani , Chisato Sakuma , Takato Takenouchi , Mitsuru Sato , Miyako Yoshioka , Noriko Yamanaka

{"title":"Establishment of c-myc-immortalized Kupffer cell line from a C57BL/6 mouse strain","authors":"Hiroshi Kitani , Chisato Sakuma , Takato Takenouchi , Mitsuru Sato , Miyako Yoshioka , Noriko Yamanaka","doi":"10.1016/j.rinim.2014.08.001","DOIUrl":"10.1016/j.rinim.2014.08.001","url":null,"abstract":"<div>We recently demonstrated in several mammalian species, a novel procedure to obtain liver-macrophages (Kupffer cells) in sufficient numbers and purity using a mixed primary culture of hepatocytes. In this study, we applied this method to the C57BL/6 mouse liver and established an immortalized Kupffer cell line from this mouse strain. The hepatocytes from the C57BL/6 adult mouse liver were isolated by a two-step collagenase perfusion method and cultured in T25 culture flasks. Similar to our previous studies, the mouse hepatocytes progressively changed their morphology into a fibroblastic appearance after a few days of culture. After 7–10 days of culture, Kupffer-like cells, which were contaminants in the hepatocyte fraction at the start of the culture, actively proliferated on the mixed fibroblastic cell sheet. At this stage, a retroviral vector containing the human c-myc oncogene and neomycin resistance gene was introduced into the mixed culture. Gentle shaking of the culture flask, followed by the transfer and brief incubation of the culture supernatant, resulted in a quick and selective adhesion of Kupffer cells to a plastic dish surface. After selection with G418 and cloning by limiting dilutions, a clonal cell line (KUP5) was established. KUP5 cells displayed typical macrophage morphology and were stably passaged at 4–5 days intervals for more than 5 months, with a population doubling time of 19h. KUP5 cells are immunocytochemically positive for mouse macrophage markers, such as Mac-1, F4/80. KUP5 cells exhibited substantial phagocytosis of polystyrene microbeads and the release of inflammatory cytokines upon lipopolysaccharide stimulation. Taken together, KUP5 cells provide a useful means to study the function of Kupffer cells in vitro.</div>","PeriodicalId":89845,"journal":{"name":"Results in immunology","volume":"4 ","pages":"Pages 68-74"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.rinim.2014.08.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32799524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 30

Extracellular ATP does not induce P2X7 receptor-dependent responses in cultured renal- and liver-derived swine macrophages 在培养的肾源和肝源猪巨噬细胞中，细胞外ATP不会诱导P2X7受体依赖性反应

Results in immunology Pub Date : 2014-01-01 DOI: 10.1016/j.rinim.2014.07.002

Takato Takenouchi , Shunichi Suzuki , Hiroki Shinkai , Mitsutoshi Tsukimoto , Mitsuru Sato , Hirohide Uenishi , Hiroshi Kitani

{"title":"Extracellular ATP does not induce P2X7 receptor-dependent responses in cultured renal- and liver-derived swine macrophages","authors":"Takato Takenouchi , Shunichi Suzuki , Hiroki Shinkai , Mitsutoshi Tsukimoto , Mitsuru Sato , Hirohide Uenishi , Hiroshi Kitani","doi":"10.1016/j.rinim.2014.07.002","DOIUrl":"10.1016/j.rinim.2014.07.002","url":null,"abstract":"<div>The P2X7 receptor (P2X7R) is an ATP-gated cation channel that is abundantly expressed in monocytes/macrophages. P2X7R activation by ATP results in various cellular responses including Ca2+ influx, membrane pore formation, and cytokine secretion. Since P2X7R has low affinity for ATP, high concentrations of ATP (in the mM range) are generally required to activate this receptor in vitro. Functional expression of P2X7R has been detected in monocytes/macrophages obtained from different animal species including humans, rodents, dogs, and bovines, but so far it has not been detected in swine (Sus scrofa). In this study, we investigated the expression and functions of P2X7R in swine macrophages, which were isolated from mixed primary cultures of swine kidney or liver tissue. The P2X7R mRNA and protein expression observed in the swine macrophages was comparable to that seen in a c-myc-immortalized mouse kidney-derived clonal macrophage cell line (KM-1). However, extracellular ATP did not induce P2X7R-dependent sustained Ca2+ influx, membrane pore formation, or the secretion of the bioactive cytokine interleukin-1β in the swine macrophages, whereas these responses were clearly observed in the mouse KM-1 cells after stimulation with millimolar concentrations of ATP as a positive control. These findings suggest that the ATP/P2X7R pathway is impaired in swine macrophages at least in the culture conditions used in the present study.</div>","PeriodicalId":89845,"journal":{"name":"Results in immunology","volume":"4 ","pages":"Pages 62-67"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.rinim.2014.07.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32800667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Uptake of biodegradable poly(γ-glutamic acid) nanoparticles and antigen presentation by dendritic cells in vivo 树突状细胞对可生物降解的聚γ-谷氨酸纳米颗粒的吸收和抗原递呈

Results in immunology Pub Date : 2013-01-01 DOI: 10.1016/j.rinim.2012.11.002

Tomofumi Uto , Masaaki Toyama , Yosuke Nishi , Takami Akagi , Fumiaki Shima , Mitsuru Akashi , Masanori Baba

{"title":"Uptake of biodegradable poly(γ-glutamic acid) nanoparticles and antigen presentation by dendritic cells in vivo","authors":"Tomofumi Uto , Masaaki Toyama , Yosuke Nishi , Takami Akagi , Fumiaki Shima , Mitsuru Akashi , Masanori Baba","doi":"10.1016/j.rinim.2012.11.002","DOIUrl":"10.1016/j.rinim.2012.11.002","url":null,"abstract":"<div>Poly(γ-glutamic acid) (γ-PGA) nanoparticles (NPs) carrying antigens have been shown to induce potent antigen-specific immune responses. However, in vivo delivery of γ-PGA NPs to dendritic cells (DCs), a key regulator of immune responses, still remains unclear. In this study, γ-PGA NPs were examined for their uptake by DCs and subsequent migration from the skin to the regional lymph nodes (LNs) in mice. After subcutaneous injection of fluorescein 5-isothiocyanate (FITC)-labeled NPs or FITC-ovalbumin (OVA)-carrying NPs (FITC-OVA-NPs), DCs migrated from the skin to the LNs and maturated, resulting in the upregulation of the costimulatory molecules CD80 and CD86 and the chemokine receptor CCR7. However, the migrated DCs were not detected in the spleen. FITC-OVA-NPs were found to be taken up by skin-derived CD103+ DCs, and the processed antigen peptides were cross-presented by the major histocompatibility complex (MHC) class I molecule of DCs. Furthermore, significant activation of antigen-specific CD8+ T cells was observed in mice immunized with OVA-carrying NPs (OVA-NPs) but not with OVA alone or OVA with an aluminum adjuvant. The antigen-specific CD8+ T cells were induced within 7 days after immunization with OVA-NPs. Thus, γ-PGA NPs carrying various antigens may have great potential as an antigen-delivery system and vaccine adjuvant in vivo.</div>","PeriodicalId":89845,"journal":{"name":"Results in immunology","volume":"3 ","pages":"Pages 1-9"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.rinim.2012.11.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40286827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

Sequence analysis of a normalized cDNA library of Mytilus edulis hemocytes exposed to Vibrio splendidus LGP32 strain 脾弧菌LGP32株暴露贻贝血细胞规范化cDNA文库的序列分析

Results in immunology Pub Date : 2013-01-01 DOI: 10.1016/j.rinim.2013.04.001

Marion Tanguy , Patty McKenna , Sophie Gauthier-Clerc , Jocelyne Pellerin , Jean-Michel Danger , Ahmed Siah

{"title":"Sequence analysis of a normalized cDNA library of Mytilus edulis hemocytes exposed to Vibrio splendidus LGP32 strain","authors":"Marion Tanguy , Patty McKenna , Sophie Gauthier-Clerc , Jocelyne Pellerin , Jean-Michel Danger , Ahmed Siah","doi":"10.1016/j.rinim.2013.04.001","DOIUrl":"10.1016/j.rinim.2013.04.001","url":null,"abstract":"<div>In the past decades, reports on bivalves' pathogens and associated mortalities have steadily increased. To face pathogenic micro-organisms, bivalves rely on innate defenses established in hemocytes which are essentially based on phagocytosis and cytotoxic reactions. As a step towards a better understanding of the molecular mechanisms involved in the mussel Mytilus edulis innate immune system, we constructed and sequenced a normalized cDNA library specific to M. edulis hemocytes unchallenged (control) and challenged with Vibrio splendidus LGP32 strain for 2, 4 and 6 h. A total of 1,024,708 nucleotide reads have been generated using 454 pyrosequencing. These reads have been assembled and annotated into 19,622 sequences which we believe cover most of the M. edulis hemocytes transcriptome. These sequences were successfully assigned to biological process, cellular component, and molecular function Gene Ontology (GO) categories. Several transcripts related to immunity and stress such as some fibrinogen related proteins and Toll-like receptors, the complement C1qDC, some antioxidant enzymes and antimicrobial peptides have already been identified. In addition, Toll-like receptors signaling pathways and the lysosome and apoptosis mechanisms were compared to KEGG reference pathways. As an attempt for large scale RNA sequencing, this study focuses on identifying and annotating transcripts from M. edulis hemocytes regulated during an in vitro experimental challenge with V. splendidus. The bioinformatic analysis provided a reference transcriptome, which could be used in studies aiming to quantify the level of transcripts using high-throughput analysis such as RNA-Seq.</div>","PeriodicalId":89845,"journal":{"name":"Results in immunology","volume":"3 ","pages":"Pages 40-50"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.rinim.2013.04.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40288361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 31

Development of an ELISA detecting Tumor Protein 53-Induced Nuclear Protein 1 in serum of prostate cancer patients ELISA检测前列腺癌患者血清中肿瘤蛋白53诱导核蛋白1的建立

Results in immunology Pub Date : 2013-01-01 DOI: 10.1016/j.rinim.2013.05.002

Houda Saadi , Marion Seillier , Maria José Sandi , Sylvain Peuget , Christine Kellenberger , Gwenaëlle Gravis , Nelson J. Dusetti , Juan L. Iovanna , Palma Rocchi , Mohamed Amri , Alice Carrier

{"title":"Development of an ELISA detecting Tumor Protein 53-Induced Nuclear Protein 1 in serum of prostate cancer patients","authors":"Houda Saadi , Marion Seillier , Maria José Sandi , Sylvain Peuget , Christine Kellenberger , Gwenaëlle Gravis , Nelson J. Dusetti , Juan L. Iovanna , Palma Rocchi , Mohamed Amri , Alice Carrier","doi":"10.1016/j.rinim.2013.05.002","DOIUrl":"10.1016/j.rinim.2013.05.002","url":null,"abstract":"<div>Tumor Protein 53-Induced Nuclear Protein 1 (TP53INP1) plays an important role during cell stress response in synergy with the potent “genome-keeper” p53. In human, the gene encoding TP53INP1 is expressed at very high level in some pathological situations, such as inflammation and prostate cancer (PC). TP53INP1 overexpression in PC seems to be a worse prognostic factor, particularly predictive of biological cancer relapse, making TP53INP1 a relevant specific target for molecular therapy of Castration Resistant (CR) PC. In that context, detection of TP53INP1 in patient biological fluids is a promising diagnostic avenue. We report here successful development of a new Enzyme-Linked Immunosorbent Assay (ELISA) detecting TP53INP1, taking advantage of molecular tools (monoclonal antibodies (mAbs) and recombinant proteins) generated in the laboratory during the course of basic functional investigations devoted to TP53INP1. The ELISA principle is based on a sandwich immunoenzymatic system, TP53INP1 protein being trapped by a first specific mAb coated on microplate then recognized by a second specific mAb. This new assay allows specific detection of TP53INP1 in serum of several PC patients. This breakthrough paves the way towards investigation of a large cohort of patients and assessment of clinical applications of TP53INP1 dosage.</div>","PeriodicalId":89845,"journal":{"name":"Results in immunology","volume":"3 ","pages":"Pages 51-56"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.rinim.2013.05.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40288362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

OX62+OX6+OX35+ rat dendritic cells are unable to prime CD4+ T cells for an effective immune response following acute burn injury OX62+OX6+OX35+大鼠树突状细胞在急性烧伤后不能启动CD4+ T细胞产生有效的免疫反应

Results in immunology Pub Date : 2013-01-01 DOI: 10.1016/j.rinim.2013.06.001

Nadeem Fazal

{"title":"OX62+OX6+OX35+ rat dendritic cells are unable to prime CD4+ T cells for an effective immune response following acute burn injury","authors":"Nadeem Fazal","doi":"10.1016/j.rinim.2013.06.001","DOIUrl":"10.1016/j.rinim.2013.06.001","url":null,"abstract":"<div>Co-stimulatory molecules expressed on Dendritic Cells (DCs) function to coordinate an efficient immune response by T cells in the peripheral lymph nodes. We hypothesized that CD4+ T cell-mediated immune suppression following burn injury may be related to dysfunctional DCs residing in gut associated lymphoid tissues (GALT), such as Mesenteric Lymph Nodes (MLN). Therefore, we studied co-stimulatory molecules expressed on burn rat MLN DCs as an index of functional DCs that would mount an effective normal CD4+ T cell immune response. In a rat model of 30% Total Body Surface Area (TBSA) scald burn, OX62+OX6+OX35+ DCs and CD4+ T cells were isolated from MLN of day 3 post-burn and sham control rats. DCs were tested for their expression of co-stimulatory molecules, and prime CD4+ T cell (DC:CD4+T cell co-culture assays) to determine an effector immune response such as CD4+ T cell proliferation. The surface receptor expressions of MLN DCs co-stimulatory molecules, i.e., MHC-II, CD40, CD80 (B7-1), and CD86 (B7-2) were determined by Flow cytometry (quantitatively) and confocal microscopy (qualitatively). Tritiated thymidine and CFDA-SE determined CD4+ T cell proliferation following co-incubation with DCs. Cytokine milieu of MLN (IL-12 and IL-10) was assessed by mRNA determination by RT-PCR. The results showed down-regulated expressions of co-stimulatory markers (CD80, CD86, CD40 and MHC-II) of MLN DCs obtained from burn-injured rats, as well as lack of ability of these burn-induced DCs to stimulate CD4+ T cell proliferation in co-culture assays, as compared to the sham rats. Moreover, anti-CD40 stimulation of affected burn MLN DCs did not reverse this alteration. Furthermore, a marked up-regulation of mRNA IL-10 and down-regulation of mRNA IL-12 in burn MLN as compared to sham animals was also observed. To surmise, the data indicated that dysfunctional OX62+OX6+OX35+ rat MLN DCs may contribute to CD4+ T-cell-mediated immune suppression observed following acute burn injury.</div>","PeriodicalId":89845,"journal":{"name":"Results in immunology","volume":"3 ","pages":"Pages 64-72"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.rinim.2013.06.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40288364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Complement regulator C4BP binds to Staphylococcus aureus surface proteins SdrE and Bbp inhibiting bacterial opsonization and killing 补体调节剂C4BP与金黄色葡萄球菌表面蛋白SdrE和Bbp结合，抑制细菌的调理和杀伤

Results in immunology Pub Date : 2013-01-01 DOI: 10.1016/j.rinim.2013.10.004

Pamela S. Hair , Caitlin K. Foley , Neel K. Krishna , Julius O. Nyalwidhe , Joan A. Geoghegan , Timothy J. Foster , Kenji M. Cunnion

{"title":"Complement regulator C4BP binds to Staphylococcus aureus surface proteins SdrE and Bbp inhibiting bacterial opsonization and killing","authors":"Pamela S. Hair , Caitlin K. Foley , Neel K. Krishna , Julius O. Nyalwidhe , Joan A. Geoghegan , Timothy J. Foster , Kenji M. Cunnion","doi":"10.1016/j.rinim.2013.10.004","DOIUrl":"10.1016/j.rinim.2013.10.004","url":null,"abstract":"<div>Staphylococcus aureus is a premier human pathogen and the most common cause of osteoarticular, wound, and implanted device infections. We recently demonstrated S. aureus efficiently binds the classical complement regulator C4b-binding protein (C4BP) inhibiting antibody-initiated complement-mediated opsonization. Here we identify S. aureus surface protein SdrE as a C4BP-binding protein. Recombinant SdrE and recombinant bone sialoprotein-binding protein (Bbp), an allelic variant of SdrE, both efficiently bound to C4BP in heat-inactivated human serum. We previously described SdrE as binding alternative pathway regulator factor H. Recombinant SdrE and Bbp efficiently bound C4BP and factor H in serum without apparent interference. Gain of function studies utilizing Lactococcus lactis clones expressing SdrE or Bbp increased serum C4BP and factor H binding, compared with empty-vector control (WT) approximately 2-fold. Correspondingly, classical pathway-mediated C3-fragment opsonization and bacterial killing by human neutrophils decreased by half for L. lactis clones expressing SdrE or Bbp compared with WT. In summary, we identify SdrE and allelic variant Bbp as S. aureus surface proteins that bind the complement regulator C4BP inhibiting classical pathway-mediated bacterial opsonization and killing.</div>","PeriodicalId":89845,"journal":{"name":"Results in immunology","volume":"3 ","pages":"Pages 114-121"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.rinim.2013.10.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40287768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

CD4 and CD8 T cells participate in the immune memory response against Vaccinia virus after a previous natural infection CD4和CD8 T细胞参与先前自然感染后对牛痘病毒的免疫记忆反应

Results in immunology Pub Date : 2013-01-01 DOI: 10.1016/j.rinim.2013.10.002

Daniela Carla Medeiros-Silva , Eduardo Augusto dos Santos Moreira-Silva , Juliana de Assis Silva Gomes , Flávio Guimarães da Fonseca , Rodrigo Correa-Oliveira

{"title":"CD4 and CD8 T cells participate in the immune memory response against Vaccinia virus after a previous natural infection","authors":"Daniela Carla Medeiros-Silva , Eduardo Augusto dos Santos Moreira-Silva , Juliana de Assis Silva Gomes , Flávio Guimarães da Fonseca , Rodrigo Correa-Oliveira","doi":"10.1016/j.rinim.2013.10.002","DOIUrl":"10.1016/j.rinim.2013.10.002","url":null,"abstract":"<div>The present study evaluates the immune response of memory CD4+ and CD8+ T cells from patients following a natural Vaccinia virus (VACV) infection. A total of 42 individuals were involved in the study being: 22 previously infected individuals (vaccinated or not against smallpox) and 20 non-infected individuals (vaccinated or not). A short-term in vitro stimulation with UV-inactivated VACV of whole blood cells was performed. Our study showed that previously infected individuals have a lower percentage of CD4+ T cells expressing lymph-node homing receptors (CD4+CD62L+CCR7+) and higher percentage of memory CD4+ T cells subsets (CD4+CD45ROHigh) when compared with non-infected subjects, after in vitro viral stimulation. We also showed that infected individuals presented higher percentages of CD4+ and CD8+ memory T lymphocytes expressing IFN-γ when compared to non-infected individuals. We verified that the percentage of CD4+ and CD8+ T memory cells expressing TNF-α was higher in infected and non-infected vaccinated subjects when compared with non-infected unvaccinated individual. We also observed that previously infected individuals have higher percentages of CD8+ T cells expressing lymph-node homing receptors (CCR7+ and CD62L+) and that the memory T cells expressing IFN-γ and TNF-α were at higher percentages in the whole blood cells from infected and non-infected vaccinated individuals, when compared to unvaccinated non-infected subjects. Thus, our findings suggest that CD4+ and CD8+ T cells are involved in the immune memory response against Vaccinia virus natural infection.</div>","PeriodicalId":89845,"journal":{"name":"Results in immunology","volume":"3 ","pages":"Pages 104-113"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.rinim.2013.10.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40287767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6