ISRN molecular biology最新文献_第2页

Autophagy: New Questions from Recent Answers. 自噬:来自最近答案的新问题。

ISRN molecular biology Pub Date : 2012-12-30 eCollection Date: 2012-01-01 DOI: 10.5402/2012/738718

Fulvio Reggiori

引用次数: 9

The Dual Role of TGFβ in Human Cancer: From Tumor Suppression to Cancer Metastasis. TGFβ在人类癌症中的双重作用:从肿瘤抑制到癌症转移。

ISRN molecular biology Pub Date : 2012-12-24 eCollection Date: 2012-01-01 DOI: 10.5402/2012/381428

Jean-Jacques Lebrun

{"title":"The Dual Role of TGFβ in Human Cancer: From Tumor Suppression to Cancer Metastasis.","authors":"Jean-Jacques Lebrun","doi":"10.5402/2012/381428","DOIUrl":"https://doi.org/10.5402/2012/381428","url":null,"abstract":"The transforming growth factor-beta (TGFβ) superfamily encompasses widespread and evolutionarily conserved polypeptide growth factors that regulate and orchestrate growth and differentiation in all cell types and tissues. While they regulate asymmetric cell division and cell fate determination during early development and embryogenesis, TGFβ family members play a major regulatory role in hormonal and immune responses, cell growth, cell death and cell immortalization, bone formation, tissue remodeling and repair, and erythropoiesis throughout adult life. The biological and physiological functions of TGFβ, the founding member of this family, and its receptors are of central importance to human diseases, particularly cancer. By regulating cell growth, death, and immortalization, TGFβ signaling pathways exert tumor suppressor effects in normal cells and early carcinomas. Thus, it is not surprising that a high number of human tumors arise due to mutations or deletions in the genes coding for the various TGFβ signaling components. As tumors develop and progress, these protective and cytostatic effects of TGFβ are often lost. TGFβ signaling then switches to promote cancer progression, invasion, and tumor metastasis. The molecular mechanisms underlying this dual role of TGFβ in human cancer will be discussed in depth in this paper, and it will highlight the challenge and importance of developing novel therapeutic strategies specifically aimed at blocking the prometastatic arm of the TGFβ signaling pathway without affecting its tumor suppressive effects. ","PeriodicalId":89785,"journal":{"name":"ISRN molecular biology","volume":"2012 ","pages":"381428"},"PeriodicalIF":0.0,"publicationDate":"2012-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2012/381428","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34510240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 335

Assembly of the β-Barrel Outer Membrane Proteins in Gram-Negative Bacteria, Mitochondria, and Chloroplasts. 革兰氏阴性菌、线粒体和叶绿体中β-桶状外膜蛋白的组装。

ISRN molecular biology Pub Date : 2012-11-20 eCollection Date: 2012-01-01 DOI: 10.5402/2012/708203

Rajeev Misra

{"title":"Assembly of the β-Barrel Outer Membrane Proteins in Gram-Negative Bacteria, Mitochondria, and Chloroplasts.","authors":"Rajeev Misra","doi":"10.5402/2012/708203","DOIUrl":"https://doi.org/10.5402/2012/708203","url":null,"abstract":"In the last decade, there has been an explosion of publications on the assembly of β-barrel outer membrane proteins (OMPs), which carry out diverse cellular functions, including solute transport, protein secretion, and assembly of protein and lipid components of the outer membrane. Of the three outer membrane model systems-Gram-negative bacteria, mitochondria and chloroplasts-research on bacterial and mitochondrial systems has so far led the way in dissecting the β-barrel OMP assembly pathways. Many exciting discoveries have been made, including the identification of β-barrel OMP assembly machineries in bacteria and mitochondria, and potentially the core assembly component in chloroplasts. The atomic structures of all five components of the bacterial β-barrel assembly machinery (BAM) complex, except the β-barrel domain of the core BamA protein, have been solved. Structures reveal that these proteins contain domains/motifs known to facilitate protein-protein interactions, which are at the heart of the assembly pathways. While structural information has been valuable, most of our current understanding of the β-barrel OMP assembly pathways has come from genetic, molecular biology, and biochemical analyses. This paper provides a comparative account of the β-barrel OMP assembly pathways in Gram-negative bacteria, mitochondria, and chloroplasts. ","PeriodicalId":89785,"journal":{"name":"ISRN molecular biology","volume":"2012 ","pages":"708203"},"PeriodicalIF":0.0,"publicationDate":"2012-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890855/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34493547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

DNA Extraction Protocol for Plants with High Levels of Secondary Metabolites and Polysaccharides without Using Liquid Nitrogen and Phenol. 不使用液氮和苯酚的高浓度次生代谢物和多糖植物DNA提取方案。

ISRN molecular biology Pub Date : 2012-11-14 eCollection Date: 2012-01-01 DOI: 10.5402/2012/205049

Sunil Kumar Sahu, Muthusamy Thangaraj, Kandasamy Kathiresan

{"title":"DNA Extraction Protocol for Plants with High Levels of Secondary Metabolites and Polysaccharides without Using Liquid Nitrogen and Phenol.","authors":"Sunil Kumar Sahu, Muthusamy Thangaraj, Kandasamy Kathiresan","doi":"10.5402/2012/205049","DOIUrl":"https://doi.org/10.5402/2012/205049","url":null,"abstract":"Mangroves and salt marsh species are known to synthesize a wide spectrum of polysaccharides and polyphenols including flavonoids and other secondary metabolites which interfere with the extraction of pure genomic DNA. Although a plethora of plant DNA isolation protocols exist, extracting DNA from mangroves and salt marsh species is a challenging task. This study describes a rapid and reliable cetyl trimethylammonium bromide (CTAB) protocol suited specifically for extracting DNA from plants which are rich in polysaccharides and secondary metabolites, and the protocol also excludes the use of expensive liquid nitrogen and toxic phenols. Purity of extracted DNA was excellent as evident by A260/A280 ratio ranging from 1.78 to 1.84 and A260/A230 ratio was >2, which also suggested that the preparations were sufficiently free of proteins and polyphenolics/polysaccharide compounds. DNA concentration ranged from 8.8 to 9.9 μg μL(-1). The extracted DNA was amenable to RAPD, restriction digestion, and PCR amplification of plant barcode genes (matK and rbcl). The optimized method is suitable for both dry and fresh leaves. The success of this method in obtaining high-quality genomic DNA demonstrated the broad applicability of this method. ","PeriodicalId":89785,"journal":{"name":"ISRN molecular biology","volume":"2012 ","pages":"205049"},"PeriodicalIF":0.0,"publicationDate":"2012-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2012/205049","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34604830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 222

Molecular Determinants of Cav1.2 Calcium Channel Inactivation. Cav1.2钙通道失活的分子决定因素

ISRN molecular biology Pub Date : 2012-10-17 eCollection Date: 2012-01-01 DOI: 10.5402/2012/691341

Nikolai M Soldatov

引用次数: 5

Regulation of GLUT4 and Insulin-Dependent Glucose Flux. GLUT4和胰岛素依赖性葡萄糖通量的调节。

ISRN molecular biology Pub Date : 2012-10-17 eCollection Date: 2012-01-01 DOI: 10.5402/2012/856987

Ann Louise Olson

引用次数: 70

Identification and Molecular Characterization of Molluskin, a Histone-H2A-Derived Antimicrobial Peptide from Molluscs. 软体动物组蛋白h2a抗菌肽——软体动物皮的鉴定与分子特性研究。

ISRN molecular biology Pub Date : 2012-10-15 eCollection Date: 2012-01-01 DOI: 10.5402/2012/219656

Naveen Sathyan, Rosamma Philip, E R Chaithanya, P R Anil Kumar

引用次数: 36

Nucleosome Positioning. 核小体定位。

ISRN molecular biology Pub Date : 2012-10-15 eCollection Date: 2012-01-01 DOI: 10.5402/2012/245706

Hiromi Nishida

引用次数: 3

Models, Regulations, and Functions of Microtubule Severing by Katanin. 卡塔宁切断微管的模型、规律和功能。

ISRN molecular biology Pub Date : 2012-09-27 eCollection Date: 2012-01-01 DOI: 10.5402/2012/596289

Debasish Kumar Ghosh, Debdeep Dasgupta, Abhishek Guha

{"title":"Models, Regulations, and Functions of Microtubule Severing by Katanin.","authors":"Debasish Kumar Ghosh, Debdeep Dasgupta, Abhishek Guha","doi":"10.5402/2012/596289","DOIUrl":"https://doi.org/10.5402/2012/596289","url":null,"abstract":"Regulation of microtubule dynamics depends on stochastic balance between polymerization and severing process which lead to differential spatiotemporal abundance and distribution of microtubules during cell development, differentiation, and morphogenesis. Microtubule severing by a conserved AAA family protein Katanin has emerged as an important microtubule architecture modulating process in cellular functions like division, migration, shaping and so on. Regulated by several factors, Katanin manifests connective crosstalks in network motifs in regulation of anisotropic severing pattern of microtubule protofilaments in cell type and stage dependent way. Mechanisms of structural disintegration of microtubules by Katanin involve heterogeneous mechanochemical processes and sensitivity of microtubules to Katanin plays significant roles in mitosis/meiosis, neurogenesis, cilia/flagella formation, cell wall development and so on. Deregulated and uncoordinated expression of Katanin has been shown to have implications in pathophysiological conditions. In this paper, we highlight mechanistic models and regulations of microtubule severing by Katanin in context of structure and various functions of Katanin in different organisms. ","PeriodicalId":89785,"journal":{"name":"ISRN molecular biology","volume":"2012 ","pages":"596289"},"PeriodicalIF":0.0,"publicationDate":"2012-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890891/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34493545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Inner Membrane Protein YhcB Interacts with RodZ Involved in Cell Shape Maintenance in Escherichia coli. 大肠杆菌中参与细胞形状维持的细胞膜蛋白YhcB与RodZ相互作用

ISRN molecular biology Pub Date : 2012-09-03 eCollection Date: 2012-01-01 DOI: 10.5402/2012/304021

Gaochi Li, Kentaro Hamamoto, Madoka Kitakawa

引用次数: 14