ISRN molecular biology最新文献

Recent Trends in Detection of Huntingtin and Preclinical Models of Huntington's Disease. 亨廷顿蛋白检测的最新趋势和亨廷顿病的临床前模型。

ISRN molecular biology Pub Date : 2014-05-14 eCollection Date: 2014-01-01 DOI: 10.1155/2014/190976

Neelima Mantha, Nandita G Das, Sudip K Das

引用次数: 5

Retracted: Genetic Analysis and Molecular Identification of Virulence in Xanthomonas oryzae pv. oryzae Isolates. 米黄单胞菌(Xanthomonas oryzae pv)毒力遗传分析及分子鉴定。oryzae隔离。

ISRN molecular biology Pub Date : 2014-05-04 eCollection Date: 2014-01-01 DOI: 10.1155/2014/780801

Isrn Molecular Biology

引用次数: 0

Identification of QTLs for Resistance to Sclerotinia sclerotiorum in Carioca Common Bean by the Moving Away Method. 移离法鉴定加勒比菜豆抗菌核病qtl

ISRN molecular biology Pub Date : 2014-02-06 eCollection Date: 2014-01-01 DOI: 10.1155/2014/828102

Letícia A de C Lara, João B Dos Santos, Juliana S Veloso, Marcio Balestre, Filipe C Alves, Monik E Leite

{"title":"Identification of QTLs for Resistance to Sclerotinia sclerotiorum in Carioca Common Bean by the Moving Away Method.","authors":"Letícia A de C Lara, João B Dos Santos, Juliana S Veloso, Marcio Balestre, Filipe C Alves, Monik E Leite","doi":"10.1155/2014/828102","DOIUrl":"https://doi.org/10.1155/2014/828102","url":null,"abstract":"The aim of this study was to use multiple DNA markers for detection of QTLs related to resistance to white mold in an F2 population of common bean evaluated by the straw test method. The DNA from 186 F2 plants and from the parents was extracted for genotypic evaluation using SSR, AFLP, and SRAP markers. For phenotypic analysis, 186 F2:4 progenies and ten lines were evaluated, in a 14 × 14 triple lattice experimental design. The adjusted mean values of the F2:4 progenies were used for identification of QTLs by Bayesian shrinkage analysis. Significant differences were observed among the progenies for reaction to white mold. In identification of QTLs, 17 markers identified QTLs for resistance-13 SSRs and 4 AFLPs. The moving away method under the Bayesian approach proved to be efficient in the identification of QTLs when a genetic map is not used due to the low density of markers. The ME1 and BM211 markers are near the QTLs, with the effect of increasing resistance to white mold, and they have high heritability. They are thus promising for marker-assisted selection. ","PeriodicalId":89785,"journal":{"name":"ISRN molecular biology","volume":"2014 ","pages":"828102"},"PeriodicalIF":0.0,"publicationDate":"2014-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/828102","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34669191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Expression of a Mutant kcnj2 Gene Transcript in Zebrafish. kcnj2基因突变体在斑马鱼中的表达

ISRN molecular biology Pub Date : 2013-11-26 eCollection Date: 2013-01-01 DOI: 10.1155/2013/324839

Ivone U S Leong, Jonathan R Skinner, Andrew N Shelling, Donald R Love

{"title":"Expression of a Mutant kcnj2 Gene Transcript in Zebrafish.","authors":"Ivone U S Leong, Jonathan R Skinner, Andrew N Shelling, Donald R Love","doi":"10.1155/2013/324839","DOIUrl":"https://doi.org/10.1155/2013/324839","url":null,"abstract":"Long QT 7 syndrome (LQT7, also known as Andersen-Tawil syndrome) is a rare autosomal-dominant disorder that causes cardiac arrhythmias, periodic paralysis, and dysmorphic features. Mutations in the human KCNJ2 gene, which encodes for the subunit of the potassium inwardly-rectifying channel (IK1), have been associated with the disorder. The majority of mutations are considered to be dominant-negative as mutant proteins interact to limit the function of wild type KCNJ2 proteins. Several LQT7 syndrome mouse models have been created that vary in the physiological similarity to the human disease. To complement the LQT7 mouse models, we investigated the usefulness of the zebrafish as an alternative model via a transient approach. Initial bioinformatic analysis identified the zebrafish orthologue of the human KCNJ2 gene, together with a spatial expression profile that was similar to that of human. The expression of a kcnj2-12 transcript carrying an in-frame deletion of critical amino acids identified in human studies resulted in embryos that exhibited defects in muscle development, thereby affecting movement, a decrease in jaw size, pupil-pupil distance, and signs of scoliosis. These defects correspond to some phenotypes expressed by human LQT7 patients. ","PeriodicalId":89785,"journal":{"name":"ISRN molecular biology","volume":"2013 ","pages":"324839"},"PeriodicalIF":0.0,"publicationDate":"2013-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890933/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34506606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Quantification of the Spatial Organization of the Nuclear Lamina as a Tool for Cell Classification. 核膜空间组织的量化作为细胞分类的工具。

ISRN molecular biology Pub Date : 2013-11-07 eCollection Date: 2013-01-01 DOI: 10.1155/2013/374385

Christiaan H Righolt, Diana A Zatreanu, Vered Raz

{"title":"Quantification of the Spatial Organization of the Nuclear Lamina as a Tool for Cell Classification.","authors":"Christiaan H Righolt, Diana A Zatreanu, Vered Raz","doi":"10.1155/2013/374385","DOIUrl":"https://doi.org/10.1155/2013/374385","url":null,"abstract":"The nuclear lamina is the structural scaffold of the nuclear envelope that plays multiple regulatory roles in chromatin organization and gene expression as well as a structural role in nuclear stability. The lamina proteins, also referred to as lamins, determine nuclear lamina organization and define the nuclear shape and the structural integrity of the cell nucleus. In addition, lamins are connected with both nuclear and cytoplasmic structures forming a dynamic cellular structure whose shape changes upon external and internal signals. When bound to the nuclear lamina, the lamins are mobile, have an impact on the nuclear envelop structure, and may induce changes in their regulatory functions. Changes in the nuclear lamina shape cause changes in cellular functions. A quantitative description of these structural changes could provide an unbiased description of changes in cellular function. In this review, we describe how changes in the nuclear lamina can be measured from three-dimensional images of lamins at the nuclear envelope, and we discuss how structural changes of the nuclear lamina can be used for cell classification. ","PeriodicalId":89785,"journal":{"name":"ISRN molecular biology","volume":"2013 ","pages":"374385"},"PeriodicalIF":0.0,"publicationDate":"2013-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/374385","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34506607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Genetic Analysis and Molecular Identification of Virulence in Xanthomonas oryzae pv. oryzae Isolates. 黄单胞菌（Xanthomonas oryzae pv. oryzae）分离株病毒性的遗传分析和分子鉴定。

ISRN molecular biology Pub Date : 2013-09-08 eCollection Date: 2013-01-01 DOI: 10.1155/2013/160157

Amos Onasanya, R O Onasanya, Abiodun A Ojo, B O Adewale

{"title":"Genetic Analysis and Molecular Identification of Virulence in Xanthomonas oryzae pv. oryzae Isolates.","authors":"Amos Onasanya, R O Onasanya, Abiodun A Ojo, B O Adewale","doi":"10.1155/2013/160157","DOIUrl":"10.1155/2013/160157","url":null,"abstract":"Bacterial leaf blight (BLB) of rice is a very destructive disease worldwide and is caused by Xanthomonas oryzae pv. oryzae (Xoo). The aim of the present study was to examine if the Xoo virulence pathotypes obtained using phenotypic pathotyping could be confirmed using molecular approach. After screening of 60 Operon primers with genomic DNA of two Xoo isolates (virulent pathotype, Vr, and mildly virulent pathotype, MVr), 12 Operon primers that gave reproducible and useful genetic information were selected and used to analyze 50 Xoo isolates from 7 West African countries. Genetic analysis revealed two major Xoo virulence genotypes (Mta and Mtb) with Mta having two subgroups (Mta1 and Mta2). Mta1 (Vr1) subgroup genotype has occurrence in six countries and Mta2 (Vr2) in three countries while Mtb genotype characterized mildly virulence (MVr) Xoo isolates present in five countries. The study revealed possible linkage and correlation between phenotypic pathotyping and molecular typing of Xoo virulence. Xoo virulence genotypes were known to exist within country and there was evidence of Xoo pathogen migration between countries. Durable resistance rice cultivars would need to overcome both Mta and Mtb Xoo virulence genotypes in order to survive after their deployment into different rice ecologies in West Africa. ","PeriodicalId":89785,"journal":{"name":"ISRN molecular biology","volume":"2013 ","pages":"160157"},"PeriodicalIF":0.0,"publicationDate":"2013-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890910/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34506604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Histone H2A Derived Antimicrobial Peptides, Harriottins, from Sicklefin Chimaera Neoharriotta pinnata (Schnakenbeck, 1931) and Its Evolutionary Divergence with respect to CO1 and Histone H2A. 组蛋白H2A衍生抗菌肽Harriottins的鉴定及其在CO1和组蛋白H2A方面的进化分化。

ISRN molecular biology Pub Date : 2013-06-02 eCollection Date: 2013-01-01 DOI: 10.1155/2013/930216

Naveen Sathyan, Rosamma Philip, E R Chaithanya, P R Anil Kumar, V N Sanjeevan, I S Bright Singh

{"title":"Characterization of Histone H2A Derived Antimicrobial Peptides, Harriottins, from Sicklefin Chimaera Neoharriotta pinnata (Schnakenbeck, 1931) and Its Evolutionary Divergence with respect to CO1 and Histone H2A.","authors":"Naveen Sathyan, Rosamma Philip, E R Chaithanya, P R Anil Kumar, V N Sanjeevan, I S Bright Singh","doi":"10.1155/2013/930216","DOIUrl":"https://doi.org/10.1155/2013/930216","url":null,"abstract":"Antimicrobial peptides (AMPs) are humoral innate immune components of fishes that provide protection against pathogenic infections. Histone derived antimicrobial peptides are reported to actively participate in the immune defenses of fishes. Present study deals with identification of putative antimicrobial sequences from the histone H2A of sicklefin chimaera, Neoharriotta pinnata. A 52 amino acid residue termed Harriottin-1, a 40 amino acid Harriottin-2, and a 21 mer Harriottin-3 were identified to possess antimicrobial sequence motif. Physicochemical properties and molecular structure of Harriottins are in agreement with the characteristic features of antimicrobial peptides, indicating its potential role in innate immunity of sicklefin chimaera. The histone H2A sequence of sicklefin chimera was found to differ from previously reported histone H2A sequences. Phylogenetic analysis based on histone H2A and cytochrome oxidase subunit-1 (CO1) gene revealed N. pinnata to occupy an intermediate position with respect to invertebrates and vertebrates. ","PeriodicalId":89785,"journal":{"name":"ISRN molecular biology","volume":"2013 ","pages":"930216"},"PeriodicalIF":0.0,"publicationDate":"2013-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/930216","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34556364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Efficient IDUA Gene Mutation Detection with Combined Use of dHPLC and Dried Blood Samples. dHPLC与干血联合检测IDUA基因突变的研究。

ISRN molecular biology Pub Date : 2013-04-21 eCollection Date: 2013-01-01 DOI: 10.1155/2013/451298

Diogo Ribeiro, Ana Cardoso, Ana Joana Duarte, Luis Vieira, Olga Amaral

{"title":"Efficient IDUA Gene Mutation Detection with Combined Use of dHPLC and Dried Blood Samples.","authors":"Diogo Ribeiro, Ana Cardoso, Ana Joana Duarte, Luis Vieira, Olga Amaral","doi":"10.1155/2013/451298","DOIUrl":"https://doi.org/10.1155/2013/451298","url":null,"abstract":"Objectives. Development of a simple mutation directed method in order to allow lowering the cost of mutation testing using an easily obtainable biological material. Assessment of the feasibility of such method was tested using a GC-rich amplicon. Design and Methods. A method of denaturing high-performance liquid chromatography (dHPLC) was improved and implemented as a technique for the detection of variants in exon 9 of the IDUA gene. The optimized method was tested in 500 genomic DNA samples obtained from dried blood spots (DBS). Results. With this dHPLC approach it was possible to detect different variants, including the common p.Trp402Ter mutation in the IDUA gene. The high GC content did not interfere with the resolution and reliability of this technique, and discrimination of G-C transversions was also achieved. Conclusion. This PCR-based dHPLC method is proved to be a rapid, a sensitive, and an excellent option for screening numerous samples obtained from DBS. Furthermore, it resulted in the consistent detection of clearly distinguishable profiles of the common p.Trp402Ter IDUA mutation with an advantageous balance of cost and technical requirements. ","PeriodicalId":89785,"journal":{"name":"ISRN molecular biology","volume":"2013 ","pages":"451298"},"PeriodicalIF":0.0,"publicationDate":"2013-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/451298","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34506608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Molecular Characterization and Phylogenetic Analysis of a Histone-Derived Antimicrobial Peptide Teleostin from the Marine Teleost Fishes, Tachysurus jella and Cynoglossus semifasciatus. 海洋硬骨鱼、水母速舌鱼和半鱼舌鱼组蛋白来源抗菌肽Teleostin的分子特征和系统发育分析。

ISRN molecular biology Pub Date : 2013-03-03 eCollection Date: 2013-01-01 DOI: 10.1155/2013/185807

E R Chaithanya, Rosamma Philip, Naveen Sathyan, P R Anil Kumar

引用次数: 17

The Not4 RING E3 Ligase: A Relevant Player in Cotranslational Quality Control. Not4 RING E3连接酶:与翻译质量控制相关的参与者。

ISRN molecular biology Pub Date : 2013-01-21 eCollection Date: 2013-01-01 DOI: 10.1155/2013/548359

Martine A Collart

{"title":"The Not4 RING E3 Ligase: A Relevant Player in Cotranslational Quality Control.","authors":"Martine A Collart","doi":"10.1155/2013/548359","DOIUrl":"https://doi.org/10.1155/2013/548359","url":null,"abstract":"The Not4 RING E3 ligase is a subunit of the evolutionarily conserved Ccr4-Not complex. Originally identified in yeast by mutations that increase transcription, it was subsequently defined as an ubiquitin ligase. Substrates for this ligase were characterized in yeast and in metazoans. Interestingly, some substrates for this ligase are targeted for polyubiquitination and degradation, while others instead are stable monoubiquitinated proteins. The former are mostly involved in transcription, while the latter are a ribosomal protein and a ribosome-associated chaperone. Consistently, Not4 and all other subunits of the Ccr4-Not complex are present in translating ribosomes. An important function for Not4 in cotranslational quality control has emerged. In the absence of Not4, the total level of polysomes is reduced. In addition, translationally arrested polypeptides, aggregated proteins, and polyubiquitinated proteins accumulate. Its role in quality control is likely to be related on one hand to its importance for the functional assembly of the proteasome and on the other hand to its association with the RNA degradation machines. Not4 is in an ideal position to signal to degradation mRNAs whose translation has been aborted, and this defines Not4 as a key player in the quality control of newly synthesized proteins. ","PeriodicalId":89785,"journal":{"name":"ISRN molecular biology","volume":"2013 ","pages":"548359"},"PeriodicalIF":0.0,"publicationDate":"2013-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/548359","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34669192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15