Biological chemistry Hoppe-Seyler最新文献_第9页

Hoppe-Seyler, Stokes and haemoglobin. 霍普-塞勒，斯托克斯和血红蛋白。

Biological chemistry Hoppe-Seyler Pub Date : 1995-08-01 DOI: 10.1515/bchm3.1995.376.8.449

M Perutz

引用次数: 10

Characterization of the epitope recognized by a monoclonal antibody directed against the largest subunit of Drosophila RNA polymerase II. 针对果蝇RNA聚合酶II最大亚基的单克隆抗体识别的表位特征。

Biological chemistry Hoppe-Seyler Pub Date : 1995-08-01 DOI: 10.1515/bchm3.1995.376.8.473

R E Kontermann, Z Liu, R A Schulze, K A Sommer, I Queitsch, S Dübel, S M Kipriyanov, F Breitling, E K Bautz

{"title":"Characterization of the epitope recognized by a monoclonal antibody directed against the largest subunit of Drosophila RNA polymerase II.","authors":"R E Kontermann, Z Liu, R A Schulze, K A Sommer, I Queitsch, S Dübel, S M Kipriyanov, F Breitling, E K Bautz","doi":"10.1515/bchm3.1995.376.8.473","DOIUrl":"https://doi.org/10.1515/bchm3.1995.376.8.473","url":null,"abstract":"The epitope recognized by monoclonal antibody MAb215 generated previously against Drosophila melanogaster RNA polymerase II was mapped to amino acid residues 806-820 of the largest, 215 kDa, subunit located in a region conserved within the largest subunits of pro- and eukaryotic RNA polymerases. The affinities of MAb215 and of a recombinant single-chain Fv fragment (scFv215) were determined for binding to the enzyme as well as the fusion protein and synthetic peptides used for epitope mapping. In addition, amino acid residues of the epitope important for binding to MAb215 were identified using peptides carrying single amino acid substitutions. The epitope is not involved in the polymerization reaction or the DNA unwinding process since no inhibitory effects of the monoclonal antibody were observed in nonspecific in vitro transcription using denatured calf thymus DNA or double stranded oligo dC-tailed T7 DNA as template. In contrast, MAb215 inhibits accurate in vitro transcription from the Krüppel gene promoter and from the adenovirus-2 major late promoter. Preincubation of template DNA with the nuclear extract had no effects on inhibition supporting the notion that the epitope does not participate directly in the formation of preinitiation complexes. The same inhibitory effects were observed using scFv215. The results provide further evidence that recombinant antibody fragments produced in Escherichia coli possess the same specificity and similar affinity as their parental antibodies and demonstrate that scFv fragments are useful tools for analysis of transcriptional processes.","PeriodicalId":8963,"journal":{"name":"Biological chemistry Hoppe-Seyler","volume":"376 8","pages":"473-81"},"PeriodicalIF":0.0,"publicationDate":"1995-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm3.1995.376.8.473","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18582862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 31

Felix Hoppe-Seyler (1825-1895) a pioneer of biochemistry and molecular biology. 费利克斯·霍普-塞勒(1825-1895)，生物化学和分子生物学的先驱。

Biological chemistry Hoppe-Seyler Pub Date : 1995-08-01

M Noyer-Weidner, W Schaffner

引用次数: 0

Molecular cloning and identification of a novel porcine cathelin-like antibacterial peptide precursor. 一种新型猪cathelin样抗菌肽前体的分子克隆与鉴定。

Biological chemistry Hoppe-Seyler Pub Date : 1995-08-01 DOI: 10.1515/bchm3.1995.376.8.507

B Strukelj, J Pungercar, G Kopitar, M Renko, B Lenarcic, S Berbić, V Turk

引用次数: 23

Lysyl-tRNA synthetase. Lysyl-tRNA合成酶。

Biological chemistry Hoppe-Seyler Pub Date : 1995-08-01 DOI: 10.1515/bchm3.1995.376.8.451

W Freist, D H Gauss

{"title":"Lysyl-tRNA synthetase.","authors":"W Freist, D H Gauss","doi":"10.1515/bchm3.1995.376.8.451","DOIUrl":"https://doi.org/10.1515/bchm3.1995.376.8.451","url":null,"abstract":"Lysyl-tRNA synthetase catalyses the formation of lysyl-transfer RNA, Lys-tRNA(Lys), which then is ready to insert lysine into proteins. Lysine is important for proteins since it is one of only two proteinogenic amino acids carrying an alkaline functional group. Seven genes of lysyl-tRNA synthetases have been localized in five organisms, and the nucleotide and the amino acid sequences have been established. The lysyl-tRNA synthetase molecules are of average chain lengths among the aminoacyl-tRNA synthetases, which range from about 300 to 1100 amino acids. Lysyl-tRNA synthetases act as dimers; in eukaryotes they can be localized in multienzyme complexes and can contain carbohydrates or lipids. Lysine tRNA is recognized by lysyl-tRNA synthetase via standard identity elements, namely anticodon region and acceptor stem. The aminoacylation follows the standard two-step mechanism. However the accuracy of selecting lysine against the other amino acids is less than average. The first threedimensional structure of a lysyl-tRNA synthetase worked out very recently, using the enzyme from the Escherichia coli lysU gene which binds one molecule of lysine, is similar to those of other class II synthetases. However, none of the reaction steps catalyzed by the enzyme is clarified to atomic resolution. Thus surprising findings might be possible. Lysyl-tRNA synthetase and its precursors as well as its substrates and products are targets and starting points of many regulation circuits, e.g. in multienzyme complex formation and function, dinucleoside polyphosphate synthesis, heat shock regulation, activation or deactivation by phosphorylation/dephosphorylation, inhibition by amino acid analogs, and generation of antibodies against lysyl-tRNA synthetase. None of these pathways is clarified completely.","PeriodicalId":8963,"journal":{"name":"Biological chemistry Hoppe-Seyler","volume":"376 8","pages":"451-72"},"PeriodicalIF":0.0,"publicationDate":"1995-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm3.1995.376.8.451","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18582861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 33

n-alkylglucosides serve as acceptors for galactosyltransferases from rat liver Golgi vesicles. 正烷基糖苷作为大鼠肝脏高尔基囊半乳糖转移酶的受体。

Biological chemistry Hoppe-Seyler Pub Date : 1995-08-01 DOI: 10.1515/bchm3.1995.376.8.501

G Pohlentz, M Trimborn, H Egge

引用次数: 1

Rat and human glutamate transporter GLAST1 stable heterologous expression, biochemical and functional characterization. 大鼠和人谷氨酸转运蛋白GLAST1的稳定异源表达、生化和功能表征。

Biological chemistry Hoppe-Seyler Pub Date : 1995-08-01

W Stoffel, R Blau

引用次数: 0

Lysosomal proteases cathepsins D, B, H, L and their inhibitors stefins A and B in head and neck cancer. 头颈癌溶酶体蛋白酶组织蛋白酶D、B、H、L及其抑制剂stefins A和B。

Biological chemistry Hoppe-Seyler Pub Date : 1995-07-01 DOI: 10.1515/bchm3.1995.376.7.401

J Kos, A Smid, M Krasovec, B Svetic, B Lenarcic, I Vrhovec, J Skrk, V Turk

{"title":"Lysosomal proteases cathepsins D, B, H, L and their inhibitors stefins A and B in head and neck cancer.","authors":"J Kos, A Smid, M Krasovec, B Svetic, B Lenarcic, I Vrhovec, J Skrk, V Turk","doi":"10.1515/bchm3.1995.376.7.401","DOIUrl":"https://doi.org/10.1515/bchm3.1995.376.7.401","url":null,"abstract":"In cytosols of tumour and normal tissue of 53 patients suffering from head and neck carcinoma cathepsins D, B, H and L were measured using quantitative immunoreactive assays (ELISA). The values of cathepsins D, B and L were significantly higher in tumour tissue, whereas cathepsin H concentration was lower in tumour than in normal tissue. Median cathepsin D values were 27 pmol (tumour tissue) vs. 12 pmol (normal tissue) per mg of total protein, median cathepsin B values were 1.25 micrograms/mg (tumour tissue) vs. 0.23 micrograms/mg (normal tissue) and median cathepsin L values were 39.8 ng/mg (tumour tissue) vs. 20.0 ng/mg (normal tissue). Median cathepsin H values were 1.05 micrograms/mg and 2.20 micrograms/mg for tumour and normal tissue, respectively. Additionally, stefin A and stefin B were measured in tumour and normal tissue samples. In contrast to the cathepsins, the concentrations of these inhibitors of cysteine proteinases was not significantly different between tumour and normal samples. The concentrations of cathepsins D, B, H and L and stefins A and B measured in head and neck tumours, were independent of standard clinical and histological prognostic factors. Significant correlation of tumour tissue values was observed between cathepsins B and L and between both stefins.","PeriodicalId":8963,"journal":{"name":"Biological chemistry Hoppe-Seyler","volume":"376 7","pages":"401-5"},"PeriodicalIF":0.0,"publicationDate":"1995-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm3.1995.376.7.401","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18581763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 96

Regulated expression of the retinoblastoma susceptibility gene in mammary carcinoma cells restores cyclin D1 expression and G1-phase control. 在乳腺癌细胞中调节视网膜母细胞瘤易感基因的表达可恢复cyclin D1的表达和g1期的控制。

Biological chemistry Hoppe-Seyler Pub Date : 1995-07-01

T Gjetting, J Lukas, J Bartek, M Strauss

{"title":"Regulated expression of the retinoblastoma susceptibility gene in mammary carcinoma cells restores cyclin D1 expression and G1-phase control.","authors":"T Gjetting, J Lukas, J Bartek, M Strauss","doi":"","DOIUrl":"","url":null,"abstract":"The product of the retinoblastoma susceptibility tumour suppressor gene, pRb, is a negative regulator of cell proliferation. In order to investigate the interaction between pRb and the cell cycle machinery in more detail, a functional Rb gene was reintroduced into the Rb-deficient human mammary carcinoma cell line Bt549. Since constitutive high level expression of Rb turned out to be difficult to maintain, the tetracycline-dependent gene expression system was used. A number of clones was generated which all showed low level expression in the noninduced state. Considerable induction rates were obtained. The low level of noninduced Rb expression was sufficient to induce the expression of cyclin D1 the level of which was not further increased by upregulation of Rb expression. Concomittantly, an increase in cell doubling time was observed due to retardation of the cell cycle in the G1-phase. The data suggest that limiting amounts of cyclin D1 determine, at least partly, the extent of growth-repressing properties of pRb. The inducible system allows for maintenance of Rb-reconstituted cells at a low level of expression and for their use in the investigation of downstream functions of pRb.","PeriodicalId":8963,"journal":{"name":"Biological chemistry Hoppe-Seyler","volume":"376 7","pages":"441-6"},"PeriodicalIF":0.0,"publicationDate":"1995-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18581769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alpha-melanocyte stimulating hormone induces collagenase/matrix metalloproteinase-1 in human dermal fibroblasts. α -黑色素细胞刺激激素诱导人真皮成纤维细胞胶原酶/基质金属蛋白酶-1。

Biological chemistry Hoppe-Seyler Pub Date : 1995-07-01 DOI: 10.1515/bchm3.1995.376.7.425

M Kiss, M Wlaschek, P Brenneisen, G Michel, C Hommel, T S Lange, D Peus, L Kemeny, A Dobozy, K Scharffetter-Kochanek

{"title":"Alpha-melanocyte stimulating hormone induces collagenase/matrix metalloproteinase-1 in human dermal fibroblasts.","authors":"M Kiss, M Wlaschek, P Brenneisen, G Michel, C Hommel, T S Lange, D Peus, L Kemeny, A Dobozy, K Scharffetter-Kochanek","doi":"10.1515/bchm3.1995.376.7.425","DOIUrl":"https://doi.org/10.1515/bchm3.1995.376.7.425","url":null,"abstract":"Recent reports have suggested that alpha-melanocyte stimulating hormone (alpha-MSH) plays an important role in untraviolet (UV) irradiation mediated skin changes including pigmentation, inflammation and connective tissue damage. alpha-MSH synthesis has been found to be induced in human keratinocytes following UV irradiation. In order to test the hypothesis whether UV induced alpha-MSH - via a paracrine loop - regulates the synthesis and the activity of collagenase/MMP-1, we studied the effects of alpha-MSH on the expression of MMP-1 and its tissue inhibitor of matrix metalloproteinases (TIMP-1). Confluent human dermal fibroblast cultures from foreskin biopsies of healthy human donors were treated with 10(-5)M, 10(-8)M and 10(-11)M alpha-MSH for 30 min. As determined by Northern blot analysis 10(-5)M and 10(-8)M alpha-MSH dose- and time-dependently induced steady state levels of MMP-1 mRNA up to 9-fold compared to untreated controls. TIMP-1 mRNA steady state levels were also slightly induced, however, the increased MMP-1/TIMP-1 ratio when normalized to beta-actin reflected an unbalanced synthesis. MMP-1 protein expression was studied with an immunofluorescence technique using a monoclonal antibody against MMP-1. After alpha-MSH treatment an increased number of fibroblasts revealed an intense perinuclear staining pattern compared to the less intense staining of control fibroblasts. The overall collagenolytic activity of supernatants from alpha-MSH treated fibroblasts was increased by 35%. Our data support the view that UV induced alpha-MSH - by the stimulation of collagenase/MMP-1 - may contribute to the loss of interstitial collagen related to cutaneous photoaging.","PeriodicalId":8963,"journal":{"name":"Biological chemistry Hoppe-Seyler","volume":"376 7","pages":"425-30"},"PeriodicalIF":0.0,"publicationDate":"1995-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm3.1995.376.7.425","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18581766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 46