Biological chemistry Hoppe-Seyler最新文献_第8页

Identification of human nonpancreatic-type ribonuclease by antibodies obtained against a synthetic peptide. 人非胰型核糖核酸酶的合成肽抗体鉴定。

Biological chemistry Hoppe-Seyler Pub Date : 1995-09-01 DOI: 10.1515/bchm3.1995.376.9.555

M I Bravo, C M Cuchillo, M V Nogués

引用次数: 1

Diversity in the properties of two sialidase isoenzymes produced by Clostridium perfringens spp. 产气荚膜梭菌两种唾液酸酶同工酶性质的差异。

Biological chemistry Hoppe-Seyler Pub Date : 1995-09-01 DOI: 10.1515/bchm3.1995.376.9.569

P Roggentin, R G Kleineidam, R Schauer

{"title":"Diversity in the properties of two sialidase isoenzymes produced by Clostridium perfringens spp.","authors":"P Roggentin, R G Kleineidam, R Schauer","doi":"10.1515/bchm3.1995.376.9.569","DOIUrl":"https://doi.org/10.1515/bchm3.1995.376.9.569","url":null,"abstract":"Clostridium perfringens produces two sialidases, one of which has a molecular mass of 71 kDa and is secreted, while the 'small', 43 kDa isoenzyme remains in the cells. The secreted, higher molecular mass sialidases of two different clostridial strains, DSM756T and A99, exhibit maximum activity at pH 5.5 and at 51 or 55 degrees C, respectively. The molecular mass of both enzymes is 71 kDa in SDS-PAGE and 63 kDa as determined by gel-filtration, which indicates the absence of subunits. Natural sialidase substrates are hydrolyzed at comparably high rates, e.g. the glycoproteins fetuin and bovine submandibular gland mucin, the homopolymer colominic acid, and the ganglioside mixture from bovine brain. The partially purified 'small' isoenzyme from C. perfringens A99 cells had similar properties to the corresponding recombinant sialidase isolated from the Escherichia coli host. It is located inside the clostridial and E. coli cells and exhibits maximum activity at pH 6.1 and 37 degrees C. A relative molecular mass of 32,000 was found with FPLC gel-filtration chromatography, while primary structure analysis yielded a value of 43,000. It differs a significantly from the 'large' isoenzyme by substrate specificity. Preferred substrates are oligosaccharides, while other, more complex sialoglycoconjugates are hydrolyzed only at very low rates. alpha 2,3-linkages are hydrolyzed much faster than alpha 2,6-bonds.","PeriodicalId":8963,"journal":{"name":"Biological chemistry Hoppe-Seyler","volume":"376 9","pages":"569-75"},"PeriodicalIF":0.0,"publicationDate":"1995-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm3.1995.376.9.569","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19542355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 42

Characterization of N-linked carbohydrate chains of the crayfish, Astacus leptodactylus hemocyanin. 螯虾(Astacus leptodactylus)血青素n链碳水化合物的表征。

Biological chemistry Hoppe-Seyler Pub Date : 1995-09-01 DOI: 10.1515/bchm3.1995.376.9.531

D Tseneklidou-Stoeter, G J Gerwig, J P Kamerling, K D Spindler

引用次数: 21

Kinetic analysis of an autocatalytic process coupled to a reversible inhibition: the inhibition of the system trypsinogen-trypsin by p-aminobenzamidine. 耦合可逆抑制的自催化过程动力学分析:对氨基苄胺对胰蛋白酶原-胰蛋白酶系统的抑制。

Biological chemistry Hoppe-Seyler Pub Date : 1995-09-01 DOI: 10.1515/bchm3.1995.376.9.577

M C Manjabacas, E Valero, M García-Moreno, R Varón

引用次数: 14

Atrial-natriuretic-peptide receptors in glomerular cryosections of renal malignant and spontaneously hypertensive rats. 肾恶性和自发性高血压大鼠肾小球冷冻切片中的心房钠尿肽受体。

Biological chemistry Hoppe-Seyler Pub Date : 1995-09-01 DOI: 10.1515/bchm3.1995.376.9.539

M Marin-Grez, C Grigelat, P Heinz-Erian, J M Heim, H G Klein

{"title":"Atrial-natriuretic-peptide receptors in glomerular cryosections of renal malignant and spontaneously hypertensive rats.","authors":"M Marin-Grez, C Grigelat, P Heinz-Erian, J M Heim, H G Klein","doi":"10.1515/bchm3.1995.376.9.539","DOIUrl":"https://doi.org/10.1515/bchm3.1995.376.9.539","url":null,"abstract":"ANP-receptors affinities (KD) and capacities (Bmax) were assayed in cryosections of glomeruli from 'malignant' hypertensive rats (2K-1C) and spontaneously hypertensive rats (PHR). Plasma ANP concentration was twofold higher in 2K-1C (P < 0.05) and PHR (P < 0.02) than in the respective controls, KD and Bmax for rANP99-126 and ANP103-123 did not differ. ANP mediated cGAMP release in 2K-1C rats was also unaffected. ANP-C glomerular receptors (i.e. displacement of tracer binding with ANP103-123) were not down-regulated and had unchanged peptide binding affinity in either kidney of rats with 'malignant' hypertension and in PHR. The difference between Bmax for rANP99-126 and Bmax for rANP103-123 (ANP-A receptor binding) indicates moderate up-regulation of ANP-A receptors in the clipped, and down-regulation in the contralateral kidney of 2K-1C (2K-1C, right vs. left, P < 0.05). Since [ANP]pl, and also Bmax and KD for ANP were similar in both hypertension models investigated, changes of the [ANP]pl/ANP-receptor system can not completely explain the marked natriuresis of rats with 'malignant' hypertension.","PeriodicalId":8963,"journal":{"name":"Biological chemistry Hoppe-Seyler","volume":"376 9","pages":"539-44"},"PeriodicalIF":0.0,"publicationDate":"1995-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm3.1995.376.9.539","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19542351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evidence of the coevolution of a snake toxin and its endogenous antitoxin cloning, sequence and expression of a serum albumin cDNA of the Chinese cobra. 中国眼镜蛇血清白蛋白cDNA的克隆、序列及表达。

Biological chemistry Hoppe-Seyler Pub Date : 1995-09-01 DOI: 10.1515/bchm3.1995.376.9.545

X Wang, B Havsteen, H Hansen

{"title":"Evidence of the coevolution of a snake toxin and its endogenous antitoxin cloning, sequence and expression of a serum albumin cDNA of the Chinese cobra.","authors":"X Wang, B Havsteen, H Hansen","doi":"10.1515/bchm3.1995.376.9.545","DOIUrl":"https://doi.org/10.1515/bchm3.1995.376.9.545","url":null,"abstract":"A full-length cDNA of the serum albumin (CSA) of the cobra (Naja naja kaouthia) was cloned from a lambda gt 11 library. It encodes a mature protein of 614 amino-acid residues homologous to the precursor of mammalian serum albumins. The 1 degree and 2 degrees structures of the CSA resemble those of the human variety. The putative toxin binding sites are mainly located in the subdomains IIA and IIIA. The relation between structural homology and function of the serum albumins (SA) is discussed. An analysis of their evolutionary tree revealed that anti-toxicity arose by < 90 amino-acid exchanges. The rate of substitution is much higher in the SA than in cytochrome C, which probably reflects the difference in evolutionary driving forces. The evolutionary period of the SA (6.7 +/- 0.1 M.Y.) significantly exceeds that of hemoglobin (5.8 M.Y.). Eight tripeptides in the nicotinic acetylcholine receptor (ACR), all flanking the putative toxin binding site, are also found in the CSA where they join to form 1 octa-, 1 penta- and 4 tripeptides, thus indicating the concerted evolution of two functionally linked proteins: toxin and antitoxin (CSA).","PeriodicalId":8963,"journal":{"name":"Biological chemistry Hoppe-Seyler","volume":"376 9","pages":"545-53"},"PeriodicalIF":0.0,"publicationDate":"1995-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm3.1995.376.9.545","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19542352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

46th Mosbacher Kolloquium. The Biochemistry and Molecular Biology of Tumor Development, Basic Science at the Doorstep of Clinical Medicine. Mosbach, April 27-29, 1995. Abstracts. Mosbacher Kolloquium 46号。肿瘤发展的生物化学和分子生物学，临床医学门口的基础科学。莫斯巴赫，1995年4月27日至29日。摘要。

Biological chemistry Hoppe-Seyler Pub Date : 1995-09-01

引用次数: 0

Generation and activity of the ternary gelatinase B/TIMP-1/LMW-stromelysin-1 complex. 三联明胶酶B/TIMP-1/LMW-stromelysin-1复合物的生成及活性研究。

Biological chemistry Hoppe-Seyler Pub Date : 1995-08-01 DOI: 10.1515/bchm3.1995.376.8.495

H Kolkenbrock, D Orgel, A Hecker-Kia, J Zimmermann, N Ulbrich

引用次数: 26

Alterations in the visual cortex receptor pattern by operant conditioning in a reward paradigm. 奖赏模式下操作性条件反射对视觉皮层受体模式的影响。

Biological chemistry Hoppe-Seyler Pub Date : 1995-08-01 DOI: 10.1515/bchm3.1995.376.8.483

B Löhrke, H Franz, R Brose, M Klein

{"title":"Alterations in the visual cortex receptor pattern by operant conditioning in a reward paradigm.","authors":"B Löhrke, H Franz, R Brose, M Klein","doi":"10.1515/bchm3.1995.376.8.483","DOIUrl":"https://doi.org/10.1515/bchm3.1995.376.8.483","url":null,"abstract":"The aim of the study was to investigate the impact of operant conditioning on the receptor pattern in the visual cortex of calves. A reward paradigm was used to induce conditioned preference for colours. Binding sites in visual cortex specimens from conditioned and naive animals were assayed in vitro on cellular basis after dissociation of the tissue by collagenase, incubation with fluorescent ligands and flow-cytometry for fluorescence analysis. The cellular counts were subdivided according to sedimentation at 200 g as well as via the flow-cytometrical histogram by size and granularity. Binding sites of dopamine D1 and D2 receptor subtypes, glucocorticoids, opioids, casein as well as glycine and N-methyl-D-aspartic acid (NMDA) were detected by fluorescent molecular probes. In displacing naloxone fluorescein from NMDA- and mu-opioid receptors NMDA and meth-enkephalin were used. Comparisons between portions of fluorescent cellular counts from visual cortex tissue of conditioned and naive animals revealed a small increase (1.2-fold, P < 0.05) in opioid receptors of large and high granulated counts, bearing > 80% D1 receptors, and a decrease (0.70-fold, P < 0.05) in less granulated counts with variable portion of D1 receptors. Conditioning resulted in higher and lower displacing rates by meth-enkephalin and NMDA, resp., and in a reduce in counts with dopamine D1 (0.8-fold, P < 0.05), glycine and glucocorticoid binding sites (0.6-fold in both cases, P < 0.01). A tendency of elevated phagocyte marker expression occurred in high granulated counts. The data suggest that conditioning is accompanied with significant and in part marked change in binding sites studied. Induction of scavenger activity may parallel this process. As cellular portion with glycine and glucocorticoid receptors were most markedly altered by conditioning, the neurochemical needs of the used paradigm seem to focus to motility-related functions.","PeriodicalId":8963,"journal":{"name":"Biological chemistry Hoppe-Seyler","volume":"376 8","pages":"483-93"},"PeriodicalIF":0.0,"publicationDate":"1995-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm3.1995.376.8.483","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18582863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Posttranslational mitochondrial protein import in a homologous yeast in vitro system. 同源酵母体外系统中翻译后线粒体蛋白的导入。

Biological chemistry Hoppe-Seyler Pub Date : 1995-08-01

A Hönlinger, P Keil, R J Nelson, E A Craig, N Pfanner

引用次数: 0