Journal of biomedical graphics and computing最新文献

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Triple interest of imaging in Lemierre syndrome: A case report Lemierre综合征的三重兴趣成像1例
Journal of biomedical graphics and computing Pub Date : 2016-06-12 DOI: 10.5430/JBGC.V6N2P4
A. Judicael, Salami Fatima Adéniké, Gui-Bilé Ln, N. Paul, Touré Aboulaye
{"title":"Triple interest of imaging in Lemierre syndrome: A case report","authors":"A. Judicael, Salami Fatima Adéniké, Gui-Bilé Ln, N. Paul, Touré Aboulaye","doi":"10.5430/JBGC.V6N2P4","DOIUrl":"https://doi.org/10.5430/JBGC.V6N2P4","url":null,"abstract":"Oropharyngeal infections can sometimes be complicated by Lemierre syndrome (LS), which is a septic thrombophlebitis of the internal jugular vein. This is a diagnostic and therapeutic emergency. It must be systematically suspected in a patient presenting oropharyngeal infection, clinical signs of sepsis and cervical swelling and pain. Cervical and thoracic computed tomography is absolutely necessary with a triple interest; diagnosis, prognosis and therapy.","PeriodicalId":89580,"journal":{"name":"Journal of biomedical graphics and computing","volume":"6 1","pages":"4"},"PeriodicalIF":0.0,"publicationDate":"2016-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5430/JBGC.V6N2P4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71216386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Swyer-James syndrome with vascular ring in a child: Echocardiogrpahic diagnosis 儿童伴有血管环的斯威-詹姆斯综合征:超声心动图诊断
Journal of biomedical graphics and computing Pub Date : 2016-04-28 DOI: 10.5430/JBGC.V6N2P1
Fong-Lin Chen, Wei Chen, S. Kuo
{"title":"Swyer-James syndrome with vascular ring in a child: Echocardiogrpahic diagnosis","authors":"Fong-Lin Chen, Wei Chen, S. Kuo","doi":"10.5430/JBGC.V6N2P1","DOIUrl":"https://doi.org/10.5430/JBGC.V6N2P1","url":null,"abstract":"Swyer-James syndrome (SJS) is a relatively uncommon type of postinfectious obliterative bronchiolitis as a result of respiratory tract infection (RTI) in early childhood. Vascular ring (VR) is usually an underlying cause of RTI. When VR complicated with SJS, both diseases can be a relationship of cause and result, but have not been published in the past. Herein, we described an 8-year-old female school-age child who had history of recurrent RTI since age of 6 months. After screening echocardiographic findings of hypoplastic left pulmonary artery and aberrant right subclavian artery (ARSCA), confirmed by multisliced CT and functional evaluation by bronchoscopy and manometry. After she underwent aortopexy for ARSCA and balloon dilatation of constrictive bronchus and tracheal stenosis, and her growth pattern and pulmonary function improved progressively. Early detection by echocardiography and elimination of the associated underlying airway obstruction were mandatory for improvement of pulmonary function.","PeriodicalId":89580,"journal":{"name":"Journal of biomedical graphics and computing","volume":"6 1","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2016-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71216553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Role of percutaneous ethanol ablation in malignant liver tumours 经皮乙醇消融在恶性肝肿瘤中的作用
Journal of biomedical graphics and computing Pub Date : 2016-02-22 DOI: 10.5430/JBGC.V6N1P43
Satnam Singh, Jagjeet Singh, Pankaj Dugg, Sanjiv Sharma, V. Thakur, Kamaljeet Kaur, S. Sood, M. Vora, Prabhjyot Singh, J. Parmar
{"title":"Role of percutaneous ethanol ablation in malignant liver tumours","authors":"Satnam Singh, Jagjeet Singh, Pankaj Dugg, Sanjiv Sharma, V. Thakur, Kamaljeet Kaur, S. Sood, M. Vora, Prabhjyot Singh, J. Parmar","doi":"10.5430/JBGC.V6N1P43","DOIUrl":"https://doi.org/10.5430/JBGC.V6N1P43","url":null,"abstract":"Purpose: To study the effectiveness of image-guided percutaneous ethanol injection (PEI) to ablate malignant hepatic lesions. Methods: It was a hospital based prospective study. Study included 15 patients (8 men, 7 women); aged 40-75 years (mean age 58.3 years); involving 4 hepatocellular carcinoma (HCC) (ranging from 2.8 cm-8.0 cm in diameter) and 18 liver metastases (ranging from 0.78 cm-4.5 cm in diameter). Forty eight sessions (18 sessions for 3 HCCs and 30 for 11 liver metastases) using 99.9% ethanol injection were done under image guidance to treat 21 tumours in 14 patients. Under local anaesthesia, absolute ethanol (99.9%) was instilled into the tumour via 22 gauge spinal needle. Triple phased Contrast enhanced computed tomography (CECT) was used in all the cases to assess the treatment response. Results: The 3 patients having solitary HCC tumours measuring 2.8 cm, 6.5 cm and 8 cm were assigned into complete response, partial response and progressive disease categories respectively after PEI. Treatment response was not assessed in 1 patient. Among 11 liver metastases patients; complete response, partial response, stable disease and progressive disease was assigned in 5 (45.5%), 1 (6.6%), 2 (18.1%) and 3 (27.2%) patients respectively. Conclusions: PEI is a safe and effective technique for treating smaller liver neoplasms and less than 3 lesions for each patient as per BCLC criteria.","PeriodicalId":89580,"journal":{"name":"Journal of biomedical graphics and computing","volume":"6 1","pages":"43"},"PeriodicalIF":0.0,"publicationDate":"2016-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5430/JBGC.V6N1P43","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71216472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Oculus Rift virtual colonoscopy: introducing a new technology and initial impressions Oculus Rift虚拟结肠镜:介绍一项新技术和初步印象
Journal of biomedical graphics and computing Pub Date : 2015-12-22 DOI: 10.5430/JBGC.V6N1P34
D. Randall, P. Metherall, K. Bardhan, P. Spencer, R. Gillott, R. D. Noronha, J. Fenner
{"title":"The Oculus Rift virtual colonoscopy: introducing a new technology and initial impressions","authors":"D. Randall, P. Metherall, K. Bardhan, P. Spencer, R. Gillott, R. D. Noronha, J. Fenner","doi":"10.5430/JBGC.V6N1P34","DOIUrl":"https://doi.org/10.5430/JBGC.V6N1P34","url":null,"abstract":"Introduction: Virtual colonoscopy has proven to be a reliable, sensitive technique and is now widely adopted in routine clinical practice. We present a novel extension to virtual colonoscopy by incorporating the immersive Oculus Rift virtual reality technology. We describe our initial experience to explore its potential diagnostic benefit. To the best of our knowledge, this is the first Oculus Rift related publication in the radiology field. Method: A procedure for generating an Oculus Rift virtual colonoscopy (ORVC) has been created. It is derived from a CT image of an air/CO 2 inflated colon with contrast labelled faecal matter. The mucosal surface is segmented from its surroundings and subsequently meshed and rendered to produce a 3D surface. The mucosal surface is then imported into the Unity game engine along with the calculated coordinates of its centreline. Within Unity, a virtual reality camera is assigned to travel both outside and inside the colon, aided by controls allocated to an Xbox controller. Two ORVCs were created from differing quality CT data and trialled by two experienced radiologists and a gastroenterologist. Results: The ORVCs prompted an enthusiastic response from the clinicians. It was considered to offer potential for improved diagnostic power but further development is required if its full capability is to be realised. Conclusions: A mechanism for examining virtual colonoscopies within the immersive environment of the Oculus Rift has been developed. The resulting ORVCs were trialled by three clinicians with largely positive feedback providing encouragement for pursuing this work further.","PeriodicalId":89580,"journal":{"name":"Journal of biomedical graphics and computing","volume":"23 1","pages":"34"},"PeriodicalIF":0.0,"publicationDate":"2015-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5430/JBGC.V6N1P34","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71216383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 18
Improved automated vessel segmentation for diagnosing eye diseases using fundus images 改进的眼底图像自动血管分割诊断眼病
Journal of biomedical graphics and computing Pub Date : 2015-11-30 DOI: 10.5430/JBGC.V6N1P23
S. Onal, Humeyra Dabil-Karacal
{"title":"Improved automated vessel segmentation for diagnosing eye diseases using fundus images","authors":"S. Onal, Humeyra Dabil-Karacal","doi":"10.5430/JBGC.V6N1P23","DOIUrl":"https://doi.org/10.5430/JBGC.V6N1P23","url":null,"abstract":"The retina can provide evidence of diseases originating in other parts of the body. Among the eye diseases that can be diagnosed through a retinal examination, age-related macular degeneration, glaucoma, and diabetic retinopathy are the most common, and they cause vision loss. Although these diseases can be diagnosed by examining blood vessels and the optic disk in retinal images, assessment of blood vessels on colored fundus images is a time-consuming and subjective process. Here, we present an automated blood vessel segmentation algorithm that facilitates the evaluation of diabetic retinopathy through assessment of blood vessel abnormalities. The blood vessels are extracted using a random forest classification model combined with wavelet features and local binary pattern texture information. Discriminant analysis is modified and used for feature selection to train the proposed classification model. The boundary of the optic disk is identified using low-pass filtering, fuzzy c-means clustering, and template matching so that it may be removed and not confound the segmentation analysis. Validation test results using three publicly available retinal image datasets demonstrated that our proposed method achieves as good or better blood vessel segmentation accuracy than the other supervised model approaches examined. Results show that the proposed scheme is able to segment the blood vessels and optic disk structures accurately (Accuracy Index) in 95.80%, 95.20%, and 97.10% of the testing Digital Retinal Images for Vessel Extraction (DRIVE), Structured Analysis of the Retina (STARE), and CHASE_DB1 image datasets respectively. The main advantage of our proposed model is that it provides robust and computationally efficient segmentation of blood vessels and the optic disk. The proposed model aims to provide supportive information for cases in which a diagnosis remains unclear following a clinical examination.","PeriodicalId":89580,"journal":{"name":"Journal of biomedical graphics and computing","volume":"6 1","pages":"23"},"PeriodicalIF":0.0,"publicationDate":"2015-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5430/JBGC.V6N1P23","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71215903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Acute intussusception on a lipoma of the left colon: report of a case 左结肠脂肪瘤致急性肠套叠1例
Journal of biomedical graphics and computing Pub Date : 2015-11-27 DOI: 10.5430/JBGC.V6N1P20
R. Ghrissi, Mohamed Habib Belhajkhlifa, M. Elghali, M. S. Jarrar, F. Hamila, M. R. Letaief
{"title":"Acute intussusception on a lipoma of the left colon: report of a case","authors":"R. Ghrissi, Mohamed Habib Belhajkhlifa, M. Elghali, M. S. Jarrar, F. Hamila, M. R. Letaief","doi":"10.5430/JBGC.V6N1P20","DOIUrl":"https://doi.org/10.5430/JBGC.V6N1P20","url":null,"abstract":"Introduction: Lipomas of the colon are rare benign tumors. Colo-colonic intussusception caused by colonic lipoma is more rare. Case report: We report a case of a 52-year-old patient presenting with recurrent colonic obstruction which occurred in recent days. Colonoscopy revealed a large submucosal mass having benign appearance. Abdominal computed tomography scan diagnosed colo-colonic intussusception due to a large lipoma of the sigmoid colon. Diagnosis was confirmed during operative reduction and resection. He had segmental left colectomy. The postoperative course was uneventful. Conclusion: Computed tomography scan and colonoscopy seem important for diagnosing both lipoma and intussusception. The treatment may be surgical or endoscopic.","PeriodicalId":89580,"journal":{"name":"Journal of biomedical graphics and computing","volume":"6 1","pages":"20"},"PeriodicalIF":0.0,"publicationDate":"2015-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71216208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Shape localization, quantification and correspondence using Region Matching Algorithm 区域匹配算法的形状定位、量化和对应
Journal of biomedical graphics and computing Pub Date : 2015-11-24 DOI: 10.5430/JBGC.V6N1P8
Faraz Janan, S. M. Brady
{"title":"Shape localization, quantification and correspondence using Region Matching Algorithm","authors":"Faraz Janan, S. M. Brady","doi":"10.5430/JBGC.V6N1P8","DOIUrl":"https://doi.org/10.5430/JBGC.V6N1P8","url":null,"abstract":"We propose a method for local, region-based matching of planar shapes, especially as those shapes that change over time. This is a problem fundamental to medical imaging, specifically the comparison over time of mammograms. The method is based on the non-emergence and non-enhancement of maxima, as well as the causality principle of integral invariant scale space. The core idea of our Region Matching Algorithm (RMA) is to divide a shape into a number of “salient” regions and then to compare all such regions for local similarity in order to quantitatively identify new growths or partial/complete occlusions. The algorithm has several advantages over commonly used methods for shape comparison of segmented regions. First, it provides improved key-point alignment for optimal shape correspondence. Second, it identifies localized changes such as new growths as well as complete/partial occlusion in corresponding regions by dividing the segmented region into sub-regions based upon the extrema that persist over a sufficient range of scales. Third, the algorithm does not depend upon the spatial locations of mammographic features and eliminates the need for registration to identify salient changes over time. Finally, the algorithm is fast to compute and requires no human intervention. We apply the method to temporal pairs of mammograms in order to detect potentially important differences between them.","PeriodicalId":89580,"journal":{"name":"Journal of biomedical graphics and computing","volume":"6 1","pages":"8"},"PeriodicalIF":0.0,"publicationDate":"2015-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5430/JBGC.V6N1P8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71216498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Quantitative assessment of atherosclerotic lesion of rabbit aorta by compressed slice analysis of magnetic resonance images ex vivo 兔主动脉动脉粥样硬化病变的离体磁共振压缩切片定量评价
Journal of biomedical graphics and computing Pub Date : 2015-09-29 DOI: 10.5430/JBGC.V6N1P1
B. Cho, D. Kholine, B. Chung, B. Odintsov
{"title":"Quantitative assessment of atherosclerotic lesion of rabbit aorta by compressed slice analysis of magnetic resonance images ex vivo","authors":"B. Cho, D. Kholine, B. Chung, B. Odintsov","doi":"10.5430/JBGC.V6N1P1","DOIUrl":"https://doi.org/10.5430/JBGC.V6N1P1","url":null,"abstract":"The objective of the present study was to evaluate a new compressed slice analysis technique of aortas ex vivo. Atherosclerosis was developed in rabbits by feeding an atherogenic diet containing 2% cholesterol and 6% peanut oil for 8 weeks. At termination, aortas were excised and imaged ex vivo by Magnetic resonance images (MRI). MRI data were acquired on a super high magnetic field vertical bore imaging scanner equipped with a Unity/Inova console, operating at 14.1 T and dedicated to small animal studies. T2-weighted coronal 2-D sections were acquired first to determine a ROI (region of interest) of the thoracic aorta using a Spin-Echo multi slice pulse sequence. Then, T2-weighted images were acquired in transversal sections of ROI slice by slice (2 mm/slice) in the total length of 4 cm. The MRI images of 20 slices were then compressed into a single image, and the resultant compressed single image was used as a quantitative characteristic of atherosclerotic lesions. By utilizing the compressed slices (CS) analysis, the imaging intensity of atherosclerotic lesions was calculated. A significant correlation was found between the quantitative CS-MRI and the aortic cholesterol content (Pearson correlation coefficient r = 0.95). The present findings demonstrate that the compressed slice analysis technique of MRI images could be applied for a quantitative assessment of the progression and/or regression of atherosclerosis.","PeriodicalId":89580,"journal":{"name":"Journal of biomedical graphics and computing","volume":"6 1","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2015-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5430/JBGC.V6N1P1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71216331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reproducibility of a method to evaluate the pelvic floor in normal pregnancy and puerperium using 3D ultrasound and 3D-Power Doppler angiography to assess endopelvic vascularization 用3D超声和3D功率多普勒血管造影评估正常妊娠和产褥期盆底血管形成的方法的可重复性
Journal of biomedical graphics and computing Pub Date : 2015-09-27 DOI: 10.5430/JBGC.V5N2P40
Juan Troyano-Luque, M. F. Borges, A. Padilla-Pérez, M. A. D. L. Rosa, José Luis Trujillo-Carrillo, S. Mastrolia, V. Engels, L. S. Frutos, T. Pérez-Medina
{"title":"Reproducibility of a method to evaluate the pelvic floor in normal pregnancy and puerperium using 3D ultrasound and 3D-Power Doppler angiography to assess endopelvic vascularization","authors":"Juan Troyano-Luque, M. F. Borges, A. Padilla-Pérez, M. A. D. L. Rosa, José Luis Trujillo-Carrillo, S. Mastrolia, V. Engels, L. S. Frutos, T. Pérez-Medina","doi":"10.5430/JBGC.V5N2P40","DOIUrl":"https://doi.org/10.5430/JBGC.V5N2P40","url":null,"abstract":"Introduction and hypothesis: We tested inter/intra-observer reproducibility of ten different three-D ultrasound (3D-US) pelvic floor variables and endopelvic vascularization using 3D-power Doppler angiography (3D-PDA) as a reliable method to assess pelvic floor status during pregnancy and puerperium. We hypothesized that the method would show good reproducibility. Materials and methods: A prospective study was conducted in two university hospitals, with 162 nulliparous pregnant women evaluated at weeks 12, 28 and 36, and at 48 hours postpartum and three months post-delivery. At each visit, we performed transperineal 3D-US and 3D-PDA of the pelvic floor. Images corresponding to 30 cases were electronically sent between the 2 observers to test the reproducibility of the results. Results: Correlation coefficients > 0.70 for all 10 variables were obtained. Intra-observer reproducibility for each observer was very good, with intra-class correlation coefficients > 0.86. Inter-observer reproducibility of urethral sphincter volume and vascularization, 3D-PDA, VI, FI and VFI measured by VOCAL technique with automatic threshold mode showed good correlation (ICC > 0.80), considered sufficiently high to be clinically applicable. Conclusions: The present study showed good reproducibility and high inter- and intra-observer correlation coefficients for all the variables used to assess the pelvic floor during normal pregnancy and puerperium, including vaginal anterior wall anchors (AWA) as new biometric measures. This makes them reliable parameters for diagnosis and monitoring of pelvic floor disorders in normal pregnancies. We found that use of the VOCAL technique with automatic threshold mode for measurements was superior to the manual mode due to its accuracy and shorter time requirement.","PeriodicalId":89580,"journal":{"name":"Journal of biomedical graphics and computing","volume":"5 1","pages":"40"},"PeriodicalIF":0.0,"publicationDate":"2015-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5430/JBGC.V5N2P40","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71216027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A new self-made digital slide scanner and microscope for imaging and quantification of fluorescent microspheres 一种自制的荧光微球成像和定量的数字切片扫描仪和显微镜
Journal of biomedical graphics and computing Pub Date : 2015-09-21 DOI: 10.5430/JBGC.V5N2P33
William Henning, Julie B Andersen, L. Højgaard, G. Greisen, I. Law, A. Thorseth, A. Christensen
{"title":"A new self-made digital slide scanner and microscope for imaging and quantification of fluorescent microspheres","authors":"William Henning, Julie B Andersen, L. Højgaard, G. Greisen, I. Law, A. Thorseth, A. Christensen","doi":"10.5430/JBGC.V5N2P33","DOIUrl":"https://doi.org/10.5430/JBGC.V5N2P33","url":null,"abstract":"Objective: A low-cost microscope slide scanner was constructed for the purpose of digital imaging of newborn piglet brain tissue and to quantify fluorescent microspheres in tissue. Methods: Using a standard digital single-lens reflex (DSLR) camera, fluorescent imaging of newborn piglet brain tissue was performed. A computer algorithm available for download was created to detect fluorescent microspheres in the brain tissue slides and to calculate regional cerebral blood flow (rCBF). The precision of the algorithm was tested by comparing with manual counting of the fluorescent microspheres. Finally, bright-field imaging was tested by adding light diffuser film. Results: Cost of the slide scanner was a fraction of the cost of a commercial slide scanner. The slide scanner was able to image a large number of tissue slides in a semiautomatic manner and provided a large field of view (FOV) of 101 mm 2 combined with a resolution of 2.9 μm. The mean difference (SD) between manual and automatic counts was in absolute numbers 0.32 (1.5) microspheres ranging from -5 to 5 microspheres per slide. The relative total difference between automatic and manual counts was -3.1%. Conclusions: A slide scanner was constructed and an automatic algorithm to detect fluorescent microspheres in tissue was developed and validated and showed an acceptable difference to “gold standard” manual counting. The slide scanner can be regarded as a low-cost alternative for researchers when digital slide imaging and quantification of fluorescent microspheres are needed.","PeriodicalId":89580,"journal":{"name":"Journal of biomedical graphics and computing","volume":"17 1","pages":"33-39"},"PeriodicalIF":0.0,"publicationDate":"2015-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5430/JBGC.V5N2P33","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71215917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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