ISRN dentistryPub Date : 2013-09-12eCollection Date: 2013-01-01DOI: 10.1155/2013/573262
Ina Koban, Marie Henrike Geisel, Birte Holtfreter, Lukasz Jablonowski, Nils-Olaf Hübner, Rutger Matthes, Kai Masur, Klaus-Dieter Weltmann, Axel Kramer, Thomas Kocher
{"title":"Synergistic Effects of Nonthermal Plasma and Disinfecting Agents against Dental Biofilms In Vitro.","authors":"Ina Koban, Marie Henrike Geisel, Birte Holtfreter, Lukasz Jablonowski, Nils-Olaf Hübner, Rutger Matthes, Kai Masur, Klaus-Dieter Weltmann, Axel Kramer, Thomas Kocher","doi":"10.1155/2013/573262","DOIUrl":"https://doi.org/10.1155/2013/573262","url":null,"abstract":"<p><p>Aim. Dental biofilms play a major role in the pathogenesis of many dental diseases. In this study, we evaluated the synergistic effect of atmospheric pressure plasma and different agents in dentistry on the reduction of biofilms. Methods and Results. We used monospecies (S. mutans) and multispecies dental biofilm models grown on titanium discs in vitro. After treatment with one of the agents, the biofilms were treated with plasma. Efficacy of treatment was determined by the number of colony forming units (CFU) and by live-dead staining. For S. mutans biofilms no colonies could be detected after treatment with NaOCl or H2O2. For multispecies biofilms the combination with plasma achieved a higher CFU reduction than each agent alone. We found an additive antimicrobial effect between argon plasma and agents irrespective of the treatment order with cultivation technique. For EDTA and octenidine, antimicrobial efficacy assessed by live-dead staining differed significantly between the two treatment orders (P < 0.05). Conclusions. The effective treatment of dental biofilms on titanium discs with atmospheric pressure plasma could be increased by adding agents in vitro. </p>","PeriodicalId":89396,"journal":{"name":"ISRN dentistry","volume":" ","pages":"573262"},"PeriodicalIF":0.0,"publicationDate":"2013-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/573262","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40263056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ISRN dentistryPub Date : 2013-09-12eCollection Date: 2013-01-01DOI: 10.1155/2013/704027
Seyed Mohsen Hasheminia, Nastaran Farhadi, Ali Shokraneh
{"title":"Effect of Patency File on Transportation and Curve Straightening in Canal Preparation with ProTaper System.","authors":"Seyed Mohsen Hasheminia, Nastaran Farhadi, Ali Shokraneh","doi":"10.1155/2013/704027","DOIUrl":"https://doi.org/10.1155/2013/704027","url":null,"abstract":"<p><p>The aim of this ex vivo study was to evaluate the effect of using a patency file on apical transportation and curve straightening during canal instrumentation with the ProTaper rotary system. Seventy permanent mandibular first molars with mesiobuccal canals, measuring 18-23 mm in length and with a 25-40° curvature (according to the Schneider method), were selected. The working lengths were determined and the teeth were mounted and divided into two experimental groups: (A) prepared by the ProTaper system without using a patency file (n = 35) and (B) prepared by the ProTaper system using a patency file (n = 35). Radiographs taken before and after the preparation were imported into Photoshop software and the apical transportation, and curve straightening were measured. Data were analyzed using independent t-test. Partial correlation analysis was performed to evaluate the relationship between the initial curvature, transportation, and curve straightening (α = 0.05). Using a patency file during canal preparation significantly decreased both apical transportation and curve straightening (P < 0.001). There were significant relationships between the angle of curvature, transportation and curve straightening in pairs (P < 0.001). Apical patency is recommended during root canal preparation with the ProTaper rotary system. </p>","PeriodicalId":89396,"journal":{"name":"ISRN dentistry","volume":" ","pages":"704027"},"PeriodicalIF":0.0,"publicationDate":"2013-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/704027","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40263057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"An in vitro evaluation of the biological effects of carbon nanotube-coated dental zirconia.","authors":"Wen Kou, Tsukasa Akasaka, Fumio Watari, Göran Sjögren","doi":"10.1155/2013/296727","DOIUrl":"https://doi.org/10.1155/2013/296727","url":null,"abstract":"<p><p>THE PURPOSE OF THIS STUDY IS TO EVALUATE FUNCTIONALIZED MULTIWALLED CARBON NANOTUBES (FMWCNTS) AS A POTENTIAL COATING MATERIAL FOR DENTAL ZIRCONIA FROM A BIOLOGICAL PERSPECTIVE: its effect on cell proliferation, viability, morphology, and the attachment of an osteoblast-like cell. Osteoblast-like (Saos-2) cells were seeded on uncoated and fMWCNT-coated zirconia discs and in culture dishes that served as controls. The seeding density was 10(4) cells/cm(2), and the cells were cultured for 6 days. Cell viability, proliferation and attachment of the Saos-2 cells were studied. The results showed that Saos-2 cells were well attached to both the uncoated and the fMWCNT-coated zirconia discs. Cell viability and proliferation on the fMWCNT-coated zirconia discs were almost the same as for the control discs. Better cell attachment was seen on the fMWCNT-coated than on the uncoated zirconia discs. In conclusion, fMWCNTs seem to be a promising coating material for zirconia-based ceramic surfaces to increase the roughness and thereby enhance the osseointegration of zirconia implants. </p>","PeriodicalId":89396,"journal":{"name":"ISRN dentistry","volume":"2013 ","pages":"296727"},"PeriodicalIF":0.0,"publicationDate":"2013-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/296727","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31724987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ISRN dentistryPub Date : 2013-08-19eCollection Date: 2013-01-01DOI: 10.1155/2013/579039
Steven Naoum, Elizabeth Martin, Ayman Ellakwa
{"title":"Long-term fluoride exchanges at restoration surfaces and effects on surface mechanical properties.","authors":"Steven Naoum, Elizabeth Martin, Ayman Ellakwa","doi":"10.1155/2013/579039","DOIUrl":"https://doi.org/10.1155/2013/579039","url":null,"abstract":"<p><p>Aim. The aim of the study was to determine whether three fluoride containing resin composites could maintain fluoride release, fluoride recharge, and mechanical stability over long-term (18-month) aging. Materials and Methods. Fluoride containing composites Beautifil II, Gradia Direct X, Tetric EvoCeram, and glass ionomer Fuji IX Extra were analyzed. Specimens of each material were fabricated for two test groups: Group 1: bimonthly fluoride release/recharge analysis (n = 5); Group 2: hardness and elastic modulus analysis (n = 6). Nanoindentation was employed at 24 hours and at 1, 3, 6, 12, and 18 months. After 18 months, each specimen was immersed (recharged) in 5000 ppm NaF gel, and fluoride rerelease, hardness, and elastic modulus were measured. Results. Beautifil II and Gradia Direct X maintained fluoride release and recharge capability throughout 18-month aging (Beautifil II > Gradia Direct X > Tetric EvoCeram). The fluoride rerelease from Beautifil II following a 10-minute NaF recharge (at 18 months) was comparable to the long-term fluoride release from Fuji IX Extra. Elastic modulus and hardness did not change significantly (P > 0.05) with fluoride release, recharge, and water aging over 18 months for all three analyzed composites. Conclusions. The long-term fluoride release, fluoride recharge, and mechanical property stability of Beautifil II and Gradia Direct X render these composites suitable for load bearing restorations in high caries risk patients. Clinical Relevance. The ability for Beautifil II and Gradia Direct X to maintain fluoride release and fluoride recharge capability, despite long-term aging, raises the potential for unrestored tooth surfaces in contact with Beautifil II or Gradia Direct X restorations to demonstrate a reduced rate of caries incidence compared to unrestored surfaces adjacent to conventional nonfluoride containing composites. </p>","PeriodicalId":89396,"journal":{"name":"ISRN dentistry","volume":"2013 ","pages":"579039"},"PeriodicalIF":0.0,"publicationDate":"2013-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/579039","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31723477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ISRN dentistryPub Date : 2013-07-28eCollection Date: 2013-01-01DOI: 10.1155/2013/605847
Lívia Andrade Vitória, Thaiane Rodrigues Aguiar, Poliana Ramos Braga Santos, Andrea Nóbrega Cavalcanti, Paula Mathias
{"title":"Changes in water sorption and solubility of dental adhesive systems after cigarette smoke.","authors":"Lívia Andrade Vitória, Thaiane Rodrigues Aguiar, Poliana Ramos Braga Santos, Andrea Nóbrega Cavalcanti, Paula Mathias","doi":"10.1155/2013/605847","DOIUrl":"https://doi.org/10.1155/2013/605847","url":null,"abstract":"<p><p>Aim. To evaluate the effect of cigarette smoke on water sorption and solubility of four adhesive systems. Materials and Methods. Sixteen disks of each adhesive system were prepared (Adper Scotchbond Multipurpose Adhesive (SA); Adper Scotchbond Multipurpose Adhesive System (Adhesive + Primer) (SAP); Adper Single Bond Plus (SB); Adper Easy One (EO)). Specimens were desiccated until a constant mass was obtained and divided into two groups (n = 8). One-half of the specimens were immersed in deionized water, while the other half were also immersed, but with daily exposure to tobacco smoke. After 21 days, disks were measured again and stored in desiccators until constant mass was achieved. Data were calculated according to ISO specifications and statistically analyzed. Results. The tobacco smoke only significantly affected the water sorption and solubility of EO. There were significant differences in both analyses among materials tested. The SB exhibited the highest water sorption, followed by EO, which demonstrated significantly higher solubility values than SB. The SA and SAP showed low water sorption and solubility, and there were no significant differences between the two. Conclusion. Regardless of smoke exposure, both simplified adhesive systems presented an inferior performance that could be related to the complex mixture of components in such versions. </p>","PeriodicalId":89396,"journal":{"name":"ISRN dentistry","volume":"2013 ","pages":"605847"},"PeriodicalIF":0.0,"publicationDate":"2013-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/605847","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31689601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ISRN dentistryPub Date : 2013-07-14eCollection Date: 2013-01-01DOI: 10.1155/2013/276070
Timucin Ari, Nilgun Ari
{"title":"The Performance of ICDAS-II Using Low-Powered Magnification with Light-Emitting Diode Headlight and Alternating Current Impedance Spectroscopy Device for Detection of Occlusal Caries on Primary Molars.","authors":"Timucin Ari, Nilgun Ari","doi":"10.1155/2013/276070","DOIUrl":"https://doi.org/10.1155/2013/276070","url":null,"abstract":"<p><p>Early detection of occlusal caries in children is challenging for the dentists, because of the morphology of pit and fissures. The aim of this study was to compare in vitro the diagnostic performance of low-powered magnification with light-emitting diode headlight (LPMLED) using ICDAS-II criteria and AC Impedance Spectroscopy (ACIS) device, on occlusal surfaces of primary molars. The occlusal surfaces of 18 extracted primary molars were examined blindly by two examiners. The teeth were sectioned and examined under light microscopy using Downer's histological criteria as gold standard. Good to excellent inter- and intraexaminer reproducibility, higher sensitivity, specificity, and AUC values were achieved by LPMLED at D1 threshold. Also the relationship between histology and LPMLED was statistically significant. In conclusion visual aids have the potential to improve the performance of early caries detection and clinical diagnostics in children. Despite its potential, ACIS device should be considered as an adjunct method in detecting caries on primary teeth. </p>","PeriodicalId":89396,"journal":{"name":"ISRN dentistry","volume":"2013 ","pages":"276070"},"PeriodicalIF":0.0,"publicationDate":"2013-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/276070","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31666838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ISRN dentistryPub Date : 2013-06-26Print Date: 2013-01-01DOI: 10.1155/2013/352621
Khaled A Al-Haddad, Yahia T Ibrahim, Ahmed M Al-Haddad, Nezar N Al-Hebshi
{"title":"Assessment of Gingival Health Status among 5- and 12-Year-Old Children in Yemen: A Cross-Sectional Study.","authors":"Khaled A Al-Haddad, Yahia T Ibrahim, Ahmed M Al-Haddad, Nezar N Al-Hebshi","doi":"10.1155/2013/352621","DOIUrl":"https://doi.org/10.1155/2013/352621","url":null,"abstract":"<p><p>Purpose. There are limited data about the gingival health status in Yemeni children. The aim, therefore, was to assess oral hygiene status and prevalence and severity of gingivitis among Yemeni preschool and school children. Materials and Methods. A total of 5396 children were included from 5 representative Yemeni governorates: Sana'a, Hajjah, Hodeida, Hadramaut, and Taiz. Five-year olds (1292) were recruited from private kindergartens while 12-year olds (4104) were selected from public primary schools. Gingival health status was assessed using the plaque index (PI), calculus index (CAI), and gingival index (GI) on the 6 Ramfjord teeth. The latter index was used to categorize gingivitis severity at the subject level. Data were analyzed using simple hypothesis testing, as well as ordinal regression. Results. The 12-year old children had significantly much higher mean PI, CAI, and GI (P < 0.001) with 78.6% presenting with gingivitis and 47.8% with moderate gingivitis. In contrast, the figures were 27.2% and 3.1% in the younger group (P < 0.001). There were significant variations according to gender, area of residence, and governorate. Regression analysis revealed that mean PI (OR = 35), mean CAI (OR = 7.7), male gender (OR = 1.6), living in rural areas (OR = 1.4), and being from Hajjah or Sana'a were independent risk factors of gingivitis severity in the older group. For the 5-year olds, the determinants were mean PI (OR = 122), male gender (OR = 1.4), and living in Sana'a or Taiz. Conclusions. Bad oral hygiene and moderate gingivitis are highly prevalent among Yemeni preschool and school children. Geographical location appeared as important independent risk factors of gingival inflammation. </p>","PeriodicalId":89396,"journal":{"name":"ISRN dentistry","volume":"2013 ","pages":"352621"},"PeriodicalIF":0.0,"publicationDate":"2013-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/352621","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31601166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ISRN dentistryPub Date : 2013-06-24eCollection Date: 2013-01-01DOI: 10.1155/2013/959813
Mohammad Yazdizadeh, Zeinab Bouzarjomehri, Navid Khalighinejad, Leyli Sadri
{"title":"Evaluation of Apical Microleakage in Open Apex Teeth Using MTA Apical Plug in Different Sessions.","authors":"Mohammad Yazdizadeh, Zeinab Bouzarjomehri, Navid Khalighinejad, Leyli Sadri","doi":"10.1155/2013/959813","DOIUrl":"10.1155/2013/959813","url":null,"abstract":"<p><p>Aim. To compare microleakage of apexification using MTA in one or two sessions. Materials and Methods. 88 single rooted teeth were prepared and divided into two groups then received MTA apical plug. In the first group, the teeth were immersed in normal saline for 24 hours and then backfilled with guttapercha and AH26 sealer. In the second group, the teeth were obturated immediately after receiving apical plug. Four positive and four negative controls were selected. All specimens were placed in 1% methylene blue and decalcified in 5% nitric acid and finally were placed in methyl salicylate until getting transparent. All teeth were visualized for assessment of dye penetration under stereo dissecting microscope. Results. 36 and 35 teeth showed dye leakage in the first and second groups. Dye penetration into the entire canal length was confirmed in the positive control group, and in the negative control group no dye penetration was seen. Mean dye penetration in the first and second group was 5813 and 9152 μ m. t-test revealed a significant difference between dye penetrations of two groups (P < 0.05). Conclusion. MTA requires adequate time for setting in the presence of the moisture, and final obturation should be delayed until final setting of MTA. </p>","PeriodicalId":89396,"journal":{"name":"ISRN dentistry","volume":"2013 ","pages":"959813"},"PeriodicalIF":0.0,"publicationDate":"2013-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3826560/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31907435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comment on \"Evaluation of Manual and Two-Rotary NiTi Retreatment Systems in Removing Gutta-Percha Obturated with Two Root Canal Sealers\".","authors":"Seema K Dixit, Varun Arora, Kapil Loomba, Ashutosh Dixit, Ridhima Birmani Gaunkar, Bhaskar Agarwal, Alok Misra, Narendra Kumar Gupta","doi":"10.1155/2013/694027","DOIUrl":"https://doi.org/10.1155/2013/694027","url":null,"abstract":"<p><p>Proper research design, appropriate evaluation and measurement methods, use of correct statistical tests, interpretation, and inference are the essentials without which any scientific research reporting is incomplete and does not serve its proposed purpose. In this communication, some common flaws in research designing, evaluation, analysis, and inference have been explained using a published article as a reference. The purpose of the paper is to help the scientific community recognize the significance of proper research designing and planning in order to achieve the results which are worthwhile.</p>","PeriodicalId":89396,"journal":{"name":"ISRN dentistry","volume":"2013 ","pages":"694027"},"PeriodicalIF":0.0,"publicationDate":"2013-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/694027","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31518578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Oral lichen planus in thai patients has a low prevalence of human papillomavirus.","authors":"Pratanporn Arirachakaran, Jira Chansaengroj, Woradee Lurchachaiwong, Patnarin Kanjanabud, Kobkan Thongprasom, Yong Poovorawan","doi":"10.1155/2013/362750","DOIUrl":"https://doi.org/10.1155/2013/362750","url":null,"abstract":"<p><p>Background. Oral lichen planus (OLP) is a common chronic inflammatory immune-mediated disease, with an etiopathogenesis associated with cell-mediated immunological dysfunction. Viral infection has been hypothesized as a predisposing factor in the pathogenesis of this disease. Viruses may alter host cell function by inducing the abnormal expression of cellular proteins leading to disease development. However, reports on the relationship between human papillomavirus (HPV) and OLP are inconclusive. Objective. To explore the association between HPV and OLP in Thai patients. Materials and Methods. DNA was extracted from thirty-seven fresh-frozen tissue biopsy specimens from OLP lesions, and polymerase chain reaction assay for the L1 and E1 genes covering 32 types of high- and low-risk HPV was performed. Results. HPV DNA was detected in one tissue biopsy from an atrophic-type OLP lesion. All control samples were negative. Genomic sequencing of the E1 gene PCR product demonstrated that the HPV-type 16 found in the lesion is closely related to the East Asian type. Conclusion. Our data indicate a low prevalence of HPV infection in OLP lesions in Thai patients.</p>","PeriodicalId":89396,"journal":{"name":"ISRN dentistry","volume":"2013 ","pages":"362750"},"PeriodicalIF":0.0,"publicationDate":"2013-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/362750","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31503900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}