Arthritis最新文献_第2页

Combination of Interleukin-27 and MicroRNA for Enhancing Expression of Anti-Inflammatory and Proosteogenic Genes. 白细胞介素-27与MicroRNA联合增强抗炎和成骨基因表达的研究。

Arthritis Pub Date : 2017-01-01 Epub Date: 2017-02-07 DOI: 10.1155/2017/6365857

Manoel Figueiredo Neto, Marxa L Figueiredo

{"title":"Combination of Interleukin-27 and MicroRNA for Enhancing Expression of Anti-Inflammatory and Proosteogenic Genes.","authors":"Manoel Figueiredo Neto, Marxa L Figueiredo","doi":"10.1155/2017/6365857","DOIUrl":"https://doi.org/10.1155/2017/6365857","url":null,"abstract":"Remission of inflammation has become an achievable goal in inflammatory or rheumatoid arthritis (RA); however, bone erosion continues in many patients. Interleukin- (IL-) 27 regulates immune and bone cell balance and also suppresses activities of several inflammatory cell types in RA. Despite its promise, challenges to clinical translation of IL-27 have been its partial effects in vivo. Due to their ability to modulate plasticity of bone and immune cell differentiation, we examined the potential for several microRNA (miR) candidates in enhancing the effects of IL-27. Using differentiation, luciferase, and real time quantitative PCR assays, we show that IL-27 promotes osteoblast differentiation, reduces expression of osteoblast inhibitory genes, and reduces osteoclast differentiation, and results suggest a potential coordination with TGFβ/BMP/SMAD and JAK/STAT pathways. We selected miRNA regulators of these and related pathways to examine whether the effects of IL-27 could be augmented for therapeutic applications. miR-29b and miR-21 augmented IL-27 proosteogenic while downregulating osteoclastogenic signals and also worked to reduce inflammatory signaling in activated macrophages, while miR-21 and miR-20b worked with IL-27 to reduce inflammatory gene expression in fibroblasts and T cells. It appears that several miRNAs can be utilized to enhance IL-27's impact on modulating osteogenesis and reducing proinflammatory signaling.","PeriodicalId":89323,"journal":{"name":"Arthritis","volume":"2017 ","pages":"6365857"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2017/6365857","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34787594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Knee Fat Pad Volumes in Patients with Hemophilia and Their Relationship with Osteoarthritis. 血友病患者的膝关节脂肪垫体积及其与骨关节炎的关系。

Arthritis Pub Date : 2017-01-01 Epub Date: 2017-12-05 DOI: 10.1155/2017/1578623

Annette von Drygalski, Katherine C Rappazzo, Richard F W Barnes, Eric Y Chang

{"title":"Knee Fat Pad Volumes in Patients with Hemophilia and Their Relationship with Osteoarthritis.","authors":"Annette von Drygalski, Katherine C Rappazzo, Richard F W Barnes, Eric Y Chang","doi":"10.1155/2017/1578623","DOIUrl":"10.1155/2017/1578623","url":null,"abstract":"Hemophilic arthropathy is a progressive, disabling condition with poorly understood pathobiology. Since there is an emerging interest to study the role of intra-articular fat pad size and biology in arthritic conditions, we explored fat pad volume changes in hemophilic arthropathy and to what extent they differed from osteoarthritis. We matched a cohort of 13 adult patients with hemophilic arthropathy of the knee with age- and gender-matched cohorts without osteoarthritis (\"control cohort\") and with the same degree of radiographic osteoarthritis (\"OA cohort\") in 1 : 2 fashion. Infrapatellar fat pad (IPFP) and suprapatellar fat pad (SPFP) volumes were calculated based on magnetic resonance imaging and differences in fat pad volumes, demographics, height, weight, and osteoarthritis scores were evaluated. Fat pad volumes were positively associated with body size parameters in all three cohorts but were unaffected by the degree of osteoarthritis. While IPFP volumes did not differ between cohorts, SPFP volumes expanded disproportionally with weight in hemophilia patients. Our observations indicate that IPFPs and SPFPs behave biologically differently in response to different arthritic stimuli. The exaggerated expansion of the SPFP in hemophilia patients highlights the importance of further studying the implications of fat pad biology for progression of hemophilic arthropathy.","PeriodicalId":89323,"journal":{"name":"Arthritis","volume":"2017 ","pages":"1578623"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735625/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35759333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishing the Psychometric Properties of the ICOAP Questionnaire through Intra-Articular Treatment of Osteoarthritic Pain: Implementation for the Greek Version 通过关节内治疗骨关节炎疼痛建立ICOAP问卷的心理测量特性:希腊版本的实施

Arthritis Pub Date : 2016-03-13 DOI: 10.1155/2016/6201802

G. Manolarakis, N. Kontodimopoulos, D. Sifaki-Pistolla, D. Niakas

{"title":"Establishing the Psychometric Properties of the ICOAP Questionnaire through Intra-Articular Treatment of Osteoarthritic Pain: Implementation for the Greek Version","authors":"G. Manolarakis, N. Kontodimopoulos, D. Sifaki-Pistolla, D. Niakas","doi":"10.1155/2016/6201802","DOIUrl":"https://doi.org/10.1155/2016/6201802","url":null,"abstract":"Objectives. In this prospective study, we intend to establish the psychometric properties of ICOAP for its use in studies involving the Hellenic population. Methods. SF-36 Health Survey was used as a standard against ICOAP scores from a sample of 89 patients (mean age: 71.07, 69 females) with hip and knee OA pain who underwent 2 treatment cycles of 4 intra-articular injections of sodium hyaluronate, separated by a 12-week medication-free time interval. Both questionnaires were filled twice with no missing data during follow-up. Results. ROC analysis accomplished ICOAP's criterion-related validation. Wilcoxon Signed-Rank Test and paired samples t-test endorsed ICOAP's responsiveness along with Effect Size values, standard response mean, and Relative Efficiency. Comparisons between the areas under curves (AUC) on ROC plots established external responsiveness. Cronbach's-alpha value favored ICOAP's internal consistency. This, along with intraclass correlation, results in both advocated reliability and content validity. Interitem discrimination was demonstrated by the ease of completion of ICOAP as well as the degree of familiarity with it. These findings inaugurated construct validity in collaboration with Spearman's and One-Way ANOVA results. Conclusions. ICOAP is a valid, reliable, and responsive QoL instrument and suitable for studies of osteoarthritic joint pain in the Greek setting.","PeriodicalId":89323,"journal":{"name":"Arthritis","volume":"2016 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/6201802","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64466044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

The Combination of the Tunnel View and Weight-Bearing Anteroposterior Radiographs Improves the Detection of Knee Arthritis 隧道透视和负重正位x线片的结合提高了膝关节关节炎的检出率

Arthritis Pub Date : 2016-01-26 DOI: 10.1155/2016/9786924

Oladapo M. Babatunde, J. Danoff, David A. Patrick, Jonathan H. Lee, Jonathan K. Kazam, W. Macaulay

{"title":"The Combination of the Tunnel View and Weight-Bearing Anteroposterior Radiographs Improves the Detection of Knee Arthritis","authors":"Oladapo M. Babatunde, J. Danoff, David A. Patrick, Jonathan H. Lee, Jonathan K. Kazam, W. Macaulay","doi":"10.1155/2016/9786924","DOIUrl":"https://doi.org/10.1155/2016/9786924","url":null,"abstract":"Imaging used for the evaluation of knee pain has historically included weight-bearing anteroposterior (AP), lateral, and sunrise radiographs. We wished to evaluate the utility of adding the weight-bearing (WB) posteroanterior (PA) view of the knee in flexion. We hypothesize that (1) the WB tunnel view can detect radiographic osteoarthritis (OA) not visualized on the WB AP, (2) the combination of the AP and tunnel view increases the radiographic detection of OA, and (3) this may provide additional information to the clinician evaluating knee pain. We retrospectively reviewed the WB AP and tunnel view radiographs of 100 knees (74 patients) presenting with knee pain and analyzed for evidence of arthritis. The combination of the WB tunnel view and WB AP significantly increased the detection of joint space narrowing in the lateral (p < 0.001) and medial (p = 0.006) compartments over the AP view alone. The combined views significantly improved the identification of medial subchondral cysts (p = 0.022), sclerosis of the lateral tibial plateau (p = 0.041), and moderate-to-large osteophytes in the medial compartment (p = 0.012), intercondylar notch (p < 0.001), and tibial spine (p < 0.001). The WB tunnel view is an effective tool to provide additional information on affected compartments in the painful knee, not provided by the AP image alone.","PeriodicalId":89323,"journal":{"name":"Arthritis","volume":"2016 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/9786924","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64639072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Rituximab Downregulates Gene Expression Associated with Cell Proliferation, Survival, and Proteolysis in the Peripheral Blood from Rheumatoid Arthritis Patients: A Link between High Baseline Autophagy-Related ULK1 Expression and Improved Pain Control 利妥昔单抗下调类风湿关节炎患者外周血中与细胞增殖、存活和蛋白水解相关的基因表达:高基线自噬相关ULK1表达与改善疼痛控制之间的联系

Arthritis Pub Date : 2016-01-24 DOI: 10.1155/2016/4963950

E. Tchetina, Anastasya N. Pivanova, G. Markova, G. Lukina, E. N. Aleksandrova, A. Aleksankin, S. Makarov, A. N. Kuzin

{"title":"Rituximab Downregulates Gene Expression Associated with Cell Proliferation, Survival, and Proteolysis in the Peripheral Blood from Rheumatoid Arthritis Patients: A Link between High Baseline Autophagy-Related ULK1 Expression and Improved Pain Control","authors":"E. Tchetina, Anastasya N. Pivanova, G. Markova, G. Lukina, E. N. Aleksandrova, A. Aleksankin, S. Makarov, A. N. Kuzin","doi":"10.1155/2016/4963950","DOIUrl":"https://doi.org/10.1155/2016/4963950","url":null,"abstract":"Objective. To clarify molecular mechanisms for the response to rituximab in a longitudinal study. Methods. Peripheral blood from 16 RA patients treated with rituximab for a single treatment course and 26 healthy controls, blood and knee articular cartilages from 18 patients with long-standing RA, and cartilages from 14 healthy subjects were examined. Clinical response was assessed using ESR, ACPA, CRP, RF, DAS28 levels, CD19+ B-cell counts, bone erosion, and joint space narrowing scores. Protein expression in PBMCs was quantified using ELISA. Gene expression was performed with quantitative real-time PCR. Results. A decrease (p < 0.05) in DAS28, ESR, and CRP values after rituximab treatment was associated with the downregulation of MTOR, p21, caspase 3, ULK1, TNFα, IL-1β, and cathepsin K gene expression in the peripheral blood to levels found in healthy subjects. MMP-9 expression remained significantly higher compared to controls although decreased (p < 0.05) versus baseline. A negative correlation between baseline ULK1 gene expression and the number of tender joints at the end of follow-up was observed. Conclusions. The response to rituximab was associated with decreased MTOR, p21, caspase 3, ULK1, TNFα, IL-1β, and cathepsin K gene expression compared to healthy subjects. Residual increased expression in MMP-9, IFNα, and COX2 might account for remaining inflammation and pain. High baseline ULK1 gene expression indicates a good response in respect to pain.","PeriodicalId":89323,"journal":{"name":"Arthritis","volume":"2016 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/4963950","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64404546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

RNA Interference Mediated Interleukin-1β Silencing in Inflamed Chondrocytes Decreases Target and Downstream Catabolic Responses. RNA干扰介导的白介素-1β沉默在炎症软骨细胞中降低靶标和下游分解代谢反应。

Arthritis Pub Date : 2016-01-01 Epub Date: 2016-03-17 DOI: 10.1155/2016/3484961

Kyla F Ortved, Bethany S Austin, Michael S Scimeca, Alan J Nixon

{"title":"RNA Interference Mediated Interleukin-1β Silencing in Inflamed Chondrocytes Decreases Target and Downstream Catabolic Responses.","authors":"Kyla F Ortved, Bethany S Austin, Michael S Scimeca, Alan J Nixon","doi":"10.1155/2016/3484961","DOIUrl":"https://doi.org/10.1155/2016/3484961","url":null,"abstract":"Posttraumatic activation of the catabolic cascade plays a major role in degradation of cartilage. Interleukin-1β (IL-1β), a primary instigator in the catabolic axis, is upregulated in chondrocytes following injury. IL-1β activates key degradative enzymes, including MMPs and aggrecanases, and other proinflammatory mediators such as PGE2 which contribute to ECM breakdown. Posttranscriptional silencing of IL-1β by RNA interference (RNAi) may drive a reduction in IL-1β. We hypothesized that transduction of chondrocytes using rAAV2 expressing a short hairpin RNAi motif targeting IL-1β (shIL-1β) would significantly decrease IL-1β expression and, in turn, decrease expression of other catabolic enzymes. Chondrocyte cultures were transduced with rAAV2-tdT-shIL-1β in serum-free media. The fluorescent protein, tdTomato, was used to determine transduction efficiency via flow cytometry and fluorescent microscopy. Cells were stimulated with lipopolysaccharide (LPS) 48 hours following transduction. After 24-hour stimulation, supernatants were collected for cytokine analysis, and cells lysed for gene expression analysis. IL-1β knockdown led to significantly decreased expression of IL-1β, TNF-α, and ADAMTS5. PGE2 synthesis was also significantly downregulated. Overall, effective silencing of IL-1β using rAAV2 vector expressing a short hairpin IL-1β knockdown sequence was shown. Additionally, significant downstream effects were evident, including decreased expression of TNF-α and ADAMTS5. Targeted silencing of catabolic cytokines may provide a promising treatment avenue for osteoarthritic (OA) joints. ","PeriodicalId":89323,"journal":{"name":"Arthritis","volume":"2016 ","pages":"3484961"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/3484961","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34398200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Amtolmetin: A Reappraisal of NSAID with Gastroprotection. 氨托美汀:非甾体抗炎药与胃保护的再评价。

Arthritis Pub Date : 2016-01-01 Epub Date: 2016-03-22 DOI: 10.1155/2016/7103705

Amit Garg, Ahsan Shoeb, Latha Subramanya Moodahadu, Akhilesh Sharma, Arul Gandhi, Shyam Akku

{"title":"Amtolmetin: A Reappraisal of NSAID with Gastroprotection.","authors":"Amit Garg, Ahsan Shoeb, Latha Subramanya Moodahadu, Akhilesh Sharma, Arul Gandhi, Shyam Akku","doi":"10.1155/2016/7103705","DOIUrl":"https://doi.org/10.1155/2016/7103705","url":null,"abstract":"Aim. To assess the gastrosparing effect of amtolmetin guacyl (AMG) against other nonsteroidal anti-inflammatory drugs (NSAIDs) in patients with osteo-/rheumatoid arthritis. Methods. A literature search was done in the electronic databases (PubMed, Google Scholar, Embase, and Scopus) with key words \"amtolmetin guacyl\", \"amtolmetin\", and \"arthritis\"; filters were applied to obtain publications between 01-Jan-1985 and 01-Oct-2015, which were \"clinical trials\" in osteo-/rheumatoid arthritis patients and in \"English language.\" Studies were assessed using the Jadad criteria and trials with score ≥ 3 were included in the analysis to compare the safety and efficacy of AMG against other NSAIDs. Results. Search yielded 19 publications of which 3 were included for analysis. Baseline characteristics of patients were comparable between the AMG group and other NSAIDs (diclofenac, celecoxib, and piroxicam) groups in all trials. Efficacy of AMG was similar to the other NSAIDs compared in the trials. The number of adverse events (AEs) reported was similar between both the groups; however, severe AEs reported were significantly lower in the AMG group. Of note was the significant lower number of duodenal ulcers after treatment in the AMG group. Conclusions. AMG has efficacy similar to other NSAIDs and a safer gastrointestinal AE profile when compared to the other NSAIDs. ","PeriodicalId":89323,"journal":{"name":"Arthritis","volume":"2016 ","pages":"7103705"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/7103705","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34473348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Towards Tailored Patient's Management Approach: Integrating the Modified 2010 ACR Criteria for Fibromyalgia in Multidimensional Patient Reported Outcome Measures Questionnaire. 面向量身定制的患者管理方法:在多维患者报告的结果测量问卷中整合修改的2010年纤维肌痛ACR标准。

Arthritis Pub Date : 2016-01-01 Epub Date: 2016-04-13 DOI: 10.1155/2016/5371682

Yasser El Miedany, Maha El Gaafary, Sally Youssef, Ihab Ahmed

{"title":"Towards Tailored Patient's Management Approach: Integrating the Modified 2010 ACR Criteria for Fibromyalgia in Multidimensional Patient Reported Outcome Measures Questionnaire.","authors":"Yasser El Miedany, Maha El Gaafary, Sally Youssef, Ihab Ahmed","doi":"10.1155/2016/5371682","DOIUrl":"https://doi.org/10.1155/2016/5371682","url":null,"abstract":"Objectives. To assess the validity, reliability, and responsiveness to change of a patient self-reported questionnaire combining the Widespread Pain Index and the Symptom Severity Score as well as construct outcome measures and comorbidities assessment in fibromyalgia patients. Methods. The PROMs-FM was conceptualized based on frameworks used by the WHO Quality of Life tool and the PROMIS. Initially, cognitive interviews were conducted to identify item pool of questions. Item selection and reduction were achieved based on patients as well as an interdisciplinary group of specialists. Rasch and internal consistency reliability analyses were implemented. The questionnaire included the modified ACR criteria main items (Symptom Severity Score and Widespread Pain Index), in addition to assessment of functional disability, quality of life (QoL), review of the systems, and comorbidities. Every patient completed HAQ and EQ-5D questionnaires. Results. A total of 146 fibromyalgia patients completed the questionnaire. The PROMs-FM questionnaire was reliable as demonstrated by a high standardized alpha (0.886-0.982). Content construct assessment of the functional disability and QoL revealed significant correlation (p < 0.01) with both HAQ and EQ-5D. Changes in functional disability and QoL showed significant (p < 0.01) variation with diseases activity status in response to therapy. There was higher prevalence of autonomic symptoms, CVS risk, sexual dysfunction, and falling. Conclusions. The developed PROMs-FM questionnaire is a reliable and valid instrument for assessment of fibromyalgia patients. A phased treatment regimen depending on the severity of FMS as well as preferences and comorbidities of the patient is the best approach to tailored patient management. ","PeriodicalId":89323,"journal":{"name":"Arthritis","volume":"2016 ","pages":"5371682"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/5371682","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34401654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Infrapatellar Fat Pad: An Alternative Source of Adipose-Derived Mesenchymal Stem Cells. 髌下脂肪垫:脂肪来源间充质干细胞的替代来源。

Arthritis Pub Date : 2016-01-01 Epub Date: 2016-04-26 DOI: 10.1155/2016/4019873

P Tangchitphisut, N Srikaew, S Numhom, A Tangprasittipap, P Woratanarat, S Wongsak, C Kijkunasathian, S Hongeng, I R Murray, T Tawonsawatruk

{"title":"Infrapatellar Fat Pad: An Alternative Source of Adipose-Derived Mesenchymal Stem Cells.","authors":"P Tangchitphisut, N Srikaew, S Numhom, A Tangprasittipap, P Woratanarat, S Wongsak, C Kijkunasathian, S Hongeng, I R Murray, T Tawonsawatruk","doi":"10.1155/2016/4019873","DOIUrl":"https://doi.org/10.1155/2016/4019873","url":null,"abstract":"Introduction. The Infrapatellar fat pad (IPFP) represents an emerging alternative source of adipose-derived mesenchymal stem cells (ASCs). We compared the characteristics and differentiation capacity of ASCs isolated from IPFP and SC. Materials and Methods. ASCs were harvested from either IPFP or SC. IPFPs were collected from patients undergoing total knee arthroplasty (TKA), whereas subcutaneous tissues were collected from patients undergoing lipoaspiration. Immunophenotypes of surface antigens were evaluated. Their ability to form colony-forming units (CFUs) and their differentiation potential were determined. The ASCs karyotype was evaluated. Results. There was no difference in the number of CFUs and size of CFUs between IPFP and SC sources. ASCs isolated from both sources had a normal karyotype. The mesenchymal stem cells (MSCs) markers on flow cytometry was equivalent. IPFP-ASCs demonstrated significantly higher expression of SOX-9 and RUNX-2 over ASCs isolated from SC (6.19 ± 5.56-, 0.47 ± 0.62-fold; p value = 0.047, and 17.33 ± 10.80-, 1.56 ± 1.31-fold; p value = 0.030, resp.). Discussion and Conclusion. CFU assay of IPFP-ASCs and SC-ASCs harvested by lipoaspiration technique was equivalent. The expression of key chondrogenic and osteogenic genes was increased in cells isolated from IPFP. IPFP should be considered a high quality alternative source of ASCs. ","PeriodicalId":89323,"journal":{"name":"Arthritis","volume":"2016 ","pages":"4019873"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/4019873","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34593202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 46

Sucupira as a Potential Plant for Arthritis Treatment and Other Diseases 竹属植物作为治疗关节炎和其他疾病的潜在植物

Arthritis Pub Date : 2015-11-03 DOI: 10.1155/2015/379459

J. Hoscheid, M. Cardoso

{"title":"Sucupira as a Potential Plant for Arthritis Treatment and Other Diseases","authors":"J. Hoscheid, M. Cardoso","doi":"10.1155/2015/379459","DOIUrl":"https://doi.org/10.1155/2015/379459","url":null,"abstract":"Trees of the genus Pterodon, commonly known as “sucupira-branca” or “faveira,” are native to central Brazil. The Pterodon fruits are traditionally used in ethnomedicine as an infusion, in small doses, and at regular time intervals as an antirheumatic, anti-inflammatory, tonic, and depurative agent. The various compounds present in the Pterodon class are, generally, water-insoluble and derived from the fusion of high-molecular weight pentacarbonate units. Scientific research has shown that the major compounds isolated from Pterodon species are linear and/or tetracyclic diterpenes with vouacapane skeletons that partly underlie the pharmacological activities of the fruit-derived oil. Material from Pterodon species has several biological properties, such as analgesic, anti-inflammatory, and anticancer effects. Therefore, recent studies have sought to microencapsulate these extracts to protect them from potential chemical degradation and improve their water solubility, ensuring greater stability and quality of the end products. This review presents a succinct overview of the available scientific evidence of the biological activity and toxicity of Pterodon species in addition to other important aspects, including phytochemical and technological features.","PeriodicalId":89323,"journal":{"name":"Arthritis","volume":"2015 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/379459","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64932409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27