BioFactors最新文献

{"title":"Phenolic Ethanolic Extracts of Specialty Sorghum Ameliorate Intestinal Colitis and Inflammation Induced by Dextran Sulfate Sodium in Mice","authors":"Ibtesam Sleem,&nbsp;Abby Rodriguez,&nbsp;Bingqi Chen,&nbsp;Ramasamy Perumal,&nbsp;Jaymi Peterson,&nbsp;Adina L. Santana,&nbsp;Dmitriy Smolensky,&nbsp;Vermont P. Dia","doi":"10.1002/biof.70028","DOIUrl":"https://doi.org/10.1002/biof.70028","url":null,"abstract":"<div>\u0000 \u0000 <p>Recently, sorghum [<i>Sorghum bicolor</i> (L.) Moench] has been given great attention as an excellent source of polyphenols that exhibit protective effects against multiple chronic disease models. Ulcerative colitis (UC) is strongly linked to the incidence of colon cancer and other intestinal chronic diseases. This study aimed to determine the ability of sorghum ethanolic phenolic extracts (SEPEs) to mitigate intestinal colitis and inflammation induced by dextran sulfate sodium (DSS) in a mice model. Forty C57BL/6 male mice were randomly assigned to one of the experimental groups: negative control, positive control (DSS only), and three groups given SEPEs containing (100 μg gallic acid eq/mL) from specialty brown sorghum accession SC84 grains (BSG) and leaves (BSL) from the same brown cultivar, and white sorghum grains (WSG). SEPEs-fed groups showed a significant reduction of the inflammatory cytokines including IL-6, TNF-alpha, and IL-1-beta in the plasma and colon, colonic disease activity index values, and fecal hemoglobin content compared to the DSS group (<i>p</i> ≤ 0.001). Furthermore, SEPEs mitigated neutrophil infiltration by inhibiting myeloperoxidase activity in the colon and enhancing intestinal integrity by upregulation of tight junction proteins' production such as ZO-1 and claudin-7. Histopathological results showed an improvement in mucosal structure and colon morphology under SEPE uptake. BSL extract exhibited a better effect against DSS compared to BSG and WSG. Metabolomic and enrichment analyses of plasma showed that SEPEs effectively recovered the disrupted metabolomic profiling in UC via modulating key pathways associated with colitis-related inflammation and oxidative stress such as bile acids metabolism, amino acids metabolism, and taurine and hypotaurine metabolism. SEPEs ameliorate colonic colitis and inflammation by suppressing proinflammatory cytokines production, neutrophil infiltration, and enhancement of intestinal integrity and functionality. Thus, specialty sorghum phenolics represent a potential alternative to mitigate colonic inflammation and colitis.</p>\u0000 </div>","PeriodicalId":8923,"journal":{"name":"BioFactors","volume":"51 3","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144323447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural Product Daidzin Inhibits Glioma Development via Suppressing the LRP5-Mediated GSK-3β/c-Myc Signaling Pathway","authors":"Yijing Pan,&nbsp;Shunshun Wang,&nbsp;Guoliang Duan,&nbsp;Jiaqin Wu,&nbsp;Fan Feng,&nbsp;Lin Chen,&nbsp;Anzheng Li,&nbsp;Kang Xu,&nbsp;Chunli Wang,&nbsp;Shibing Fan","doi":"10.1002/biof.70025","DOIUrl":"https://doi.org/10.1002/biof.70025","url":null,"abstract":"<div>\u0000 \u0000 <p>Daidzin (DZN) is a natural flavonoid compound derived from soybeans that has recently been recognized for its potential antitumor properties. In traditional Chinese medicine, soybeans and their extracts are extensively used to prevent and treat various diseases. To evaluate the therapeutic efficacy of DZN on human glioblastoma through in vivo and in vitro experiments, and through multi-omics analyses to elucidate potential molecular mechanisms. Cell viability of LN-229 and U-87MG glioblastoma cells was assessed using the CCK-8 assay. Protein and mRNA levels of proliferation and apoptosis-related genes were analyzed via Western blotting and qPCR. Metabolomics and transcriptomics identified key pathways and targets, which were confirmed by in-cell Western blotting and expression correlation analysis. The in vivo antitumor effects of DZN were evaluated in nude mice with LN-229 tumors. DZN treatment reduced cell viability, migration, and survival in a dose-dependent manner, demonstrating strong antitumor effects in both in vitro and in vivo models. Multi-omics analysis identified amino acid metabolism and ubiquitin-mediated proteolysis as key mechanisms. Bioinformatics highlighted LRP5 as a prognostic biomarker in glioblastoma. DZN decreased LRP5 activity, downregulated p-GSK-3<i>β</i>, and promoted c-Myc degradation, thereby inhibiting the Wnt signaling pathway. In vivo, DZN significantly reduced tumor size and Ki67 expression. These findings highlight LRP5 as a promising therapeutic target, with DZN emerging as a potent LRP5 inhibitor and exhibiting significant antitumor effects in glioblastoma.</p>\u0000 </div>","PeriodicalId":8923,"journal":{"name":"BioFactors","volume":"51 3","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144220101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the Antiadipogenic Potential of Carotenoids From Galdieria phlegrea","authors":"Enrica Giustino,&nbsp;Paola Imbimbo,&nbsp;Jenifer Trepiana,&nbsp;Maria Puy Portillo,&nbsp;Daria Maria Monti","doi":"10.1002/biof.70027","DOIUrl":"https://doi.org/10.1002/biof.70027","url":null,"abstract":"<p>Oxidative stress, a sedentary and inactive lifestyle, or a high-fat diet are among the main causes responsible for the alteration of skin topography, a complex multifactorial disorder of the deeper layers of the skin, mainly caused by an impairment of lipid production, degradation, and storage. Natural bioactive compounds can improve skin health and appearance as well as reassure consumers' about the safety of the molecules used in cosmetics. Carotenoids from microalgae are receiving attention because of their potent antioxidant activity. Here, a carotenoid extract from <i>Galdieria phlegrea</i> residual biomass was successfully explored for its ability to inhibit in vitro skin hyperpigmentation, to interfere with 3T3-L1 adipocytes differentiation, as well as to affect de novo lipogenesis and enhance lipolysis in mature adipocytes. The same set of experiments was done in parallel on the extract obtained from fresh biomass, but no biological activity was observed. The obtainment of a high-value class of molecules in a cascade approach highlights the importance of a biorefinery approach to obtain active extracts with a concurrent lowering of the overall process costs.</p>","PeriodicalId":8923,"journal":{"name":"BioFactors","volume":"51 3","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/biof.70027","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144213980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Potential Role of Matrix Metalloproteinase in Polycystic Ovary Syndrome: Implications for Extracellular Matrix Remodeling","authors":"Saba Nikanfar,&nbsp;Christiani A. Amorim","doi":"10.1002/biof.70026","DOIUrl":"https://doi.org/10.1002/biof.70026","url":null,"abstract":"<div>\u0000 \u0000 <p>Polycystic ovary syndrome (PCOS) is a prevalent endocrine disorder and a leading cause of infertility primarily due to impaired folliculogenesis and anovulation. Central to ovarian function and follicular development is the extracellular matrix (ECM), which undergoes significant remodeling facilitated by matrix metalloproteinases (MMPs). In PCOS, the ovarian cortex becomes thickened and collagen-rich, creating a rigid environment that disrupts normal follicular growth and oocyte maturation. Altered MMP activity further complicates this scenario by impairing ECM degradation, leading to the accumulation of small, quiescent follicles, and elevated androgen levels. This review aims to explore the intricate roles of ECM and MMP alterations in PCOS pathogenesis, highlighting their impact on folliculogenesis and steroidogenesis. Understanding MMP/TIMP dynamics offers insights into potential therapeutic targets to restore normal ovarian function and improve fertility outcomes for women with PCOS.</p>\u0000 </div>","PeriodicalId":8923,"journal":{"name":"BioFactors","volume":"51 3","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Immunotherapeutic Target ANLN Promotes Renal Clear Cell Carcinoma by Regulating PI3K/Akt and P53 Signaling Pathways","authors":"Li Shan,&nbsp;Guo-jian Gu,&nbsp;Cheng-Cheng Xiang,&nbsp;Er-Dong Zuo,&nbsp;Lian Lian,&nbsp;Xu Cheng","doi":"10.1002/biof.70023","DOIUrl":"https://doi.org/10.1002/biof.70023","url":null,"abstract":"<div>\u0000 \u0000 <p>The role of Anillin (ANLN) in clear cell renal cell carcinoma (ccRCC) is not well understood. This study aimed to investigate the possible function and mechanism of ANLN in ccRCC. By using data from the Cancer Genome Atlas (TCGA), we assessed ANLN expression in various cancers. Furthermore, the GEPIA database was used to analyze survival rates, pathway enrichment, and immune infiltration. Experiments were employed to study how ANLN expression affects tumor cell apoptosis, migration, invasion, and proliferation. Protein blotting was conducted to evaluate apoptosis, proliferation, epithelial mesenchymal transition (EMT), and the expression of proteins linked to the PI3K/AKT and P53 signaling pathways. mRNA levels of ANLN were notably increased in diverse cancer tissues, as shown in the TCGA database, a finding that was also observed in ccRCC patients. Remarkably, high ANLN levels were associated with lower pathologic grades and a worse survival prognosis in ccRCC patients. In vitro experiments revealed that knockdown of ANLN in ccRCC cells led to reduced proliferation, migration, invasion, and EMT. Additionally, ANLN seemed to impact immune evasion and increase the likelihood of distant metastasis based on immune infiltration analyses. The use of PI3K/AKT pathway inhibitors or P53 signaling pathway agonists was effective in reducing the invasive behavior of ccRCC and correcting immunosuppression. Overall, ANLN could be a valuable prognostic indicator for the survival of ccRCC patients.</p>\u0000 </div>","PeriodicalId":8923,"journal":{"name":"BioFactors","volume":"51 3","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to “Untargeted metabolomics reveals biomarkers for the diagnosis of coronary artery plaques as observed by coronary cardiac computed tomography”","authors":"","doi":"10.1002/biof.70019","DOIUrl":"https://doi.org/10.1002/biof.70019","url":null,"abstract":"<p>Shmet M, Amasha M, Khattib A, Schweitzer R, Khatib S, Hamudi J, Halabi M, Khatib S. Untargeted metabolomics reveals biomarkers for the diagnosis of coronary artery plaques as observed by coronary cardiac computed tomography. Biofactors 2025;51(1):e2156. https://doi.org/10.1002/biof.2156</p><p>In the originally published article, Figure 6 repeated parts A and B. The correct figure, which incudes parts A–D, is included below.</p><p>We apologize for this error.</p>","PeriodicalId":8923,"journal":{"name":"BioFactors","volume":"51 3","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/biof.70019","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EVs Biodistribution and Antifibrotic Impact in Aged Lung Fibrosis Model","authors":"Ilias Amtil-Ouahdi,&nbsp;Fabián Vergara,&nbsp;Carlos Rio,&nbsp;Coral González-Martínez,&nbsp;Andreas Jahn,&nbsp;María Antonia Forteza-Genestra,&nbsp;Antonio Gayá,&nbsp;Javier Calvo,&nbsp;Ernest Sala-Llinas,&nbsp;Bernardino Alcázar Navarrete,&nbsp;Ana Dolores Romero-Ortiz,&nbsp;Marta Monjo,&nbsp;Johana M. Ramis,&nbsp;Francisco G. Ortega","doi":"10.1002/biof.70021","DOIUrl":"https://doi.org/10.1002/biof.70021","url":null,"abstract":"<div>\u0000 \u0000 <p>Pulmonary fibrosis (PF) is a progressive, life-threatening disease marked by excessive scarring of lung tissue. Recently, extracellular vesicles (EVs) have emerged as a promising antifibrotic therapy due to their regenerative and anti-inflammatory properties. However, the success of EV-based therapies depends on the route of administration, which can significantly influence their biodistribution and therapeutic effects. Furthermore, in PF, aging is a significant risk factor for the disease and, until today, EV treatment efficacy has not been studied in aged tissues. Specifically, we studied EVs derived from human umbilical cord mesenchymal stem cells and compared the biodistribution of these vesicles delivered via three routes: intravenous (IV), intrapleural (IP), and intratracheal (IT). A protocol was developed to set EV staining and concentration, minimizing animal use while maximizing the accuracy of results. To evaluate therapeutic effects, we conducted three experimental setups: (i) to assess their ability to reverse established fibrosis; (ii) to evaluate their effect on fibrosis progression; and (iii) to study early inflammation and macrophage polarization. Lung fibrosis and inflammation were assessed by analyzing fibrotic markers, inflammatory cytokines, collagen deposition, and bronchoalveolar lavage (BAL) fluid cell analysis, providing insights into EVs therapeutic potential in aged, fibrotic lung tissue. In the biodistribution study, IV administration was identified as the most effective route, successfully delivering EVs to both normal and fibrotic lung tissues. In the therapeutic study, antifibrotic effects were observed only when EVs were administered prophylactically, before the establishment of fibrosis. Under this protocol, IV-administered EVs reduced fibrotic mRNA biomarkers, collagen deposition, inflammatory cell infiltration, and macrophage polarization in BAL, as well as altering cytokine. Our findings emphasize the critical importance of selecting the appropriate route of administration for EV-based therapies. Notably, our work with an aging model reveals that EV treatments primarily exhibit prophylactic effects, with a marked reduction in their regenerative potential compared to previous studies conducted in younger models.</p>\u0000 </div>","PeriodicalId":8923,"journal":{"name":"BioFactors","volume":"51 3","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143944834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"UPS and Kinases—Gatekeepers of the G1/S Transition","authors":"Srija Roy,&nbsp;Gouranga Saha,&nbsp;Mrinal K. Ghosh","doi":"10.1002/biof.70020","DOIUrl":"https://doi.org/10.1002/biof.70020","url":null,"abstract":"<div>\u0000 \u0000 <p>The G1/S transition is a highly regulated and pivotal checkpoint in the cell cycle, where the cell decides whether to commit to DNA replication and subsequent division or enter a non-dividing state. This checkpoint serves as a critical control point for preventing uncontrolled cell proliferation and maintaining genomic stability. The major driving force underlying the G1/S transition is the sequential activation of Cyclin-dependent kinases (CDKs), which is regulated by the coordinated binding of Cyclin partners, as well as the phosphorylation and ubiquitin-mediated degradation of both Cyclin partners and Cyclin-dependent kinase inhibitors (CKIs). Various E3 ligase families govern the timely degradation of these regulatory proteins, with their activity intricately controlled by phosphorylation events. This coordination enables the cells to efficiently translate the environmental cues and molecular signaling inputs to determine their fate. We explore the evolution of three distinct models describing the G1/S transition, highlighting how the traditional linear model is being challenged by recent paradigm shifts and conflicting findings. These advances reveal emerging complexity and unresolved questions in the field, particularly regarding how the latest insights into coordinated phosphorylation and ubiquitination-dependent degradation integrate into contemporary models of the G1/S transition.</p>\u0000 </div>","PeriodicalId":8923,"journal":{"name":"BioFactors","volume":"51 3","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-beneficial effects of resveratrol on renal epithelial cell cultures: Implications in cell differentiation","authors":"L. C. Erjavec,&nbsp;L. G. Parra,&nbsp;D. E. Sendyk,&nbsp;M. Recabarren,&nbsp;C. Herbón,&nbsp;N. O. Favale,&nbsp;S. Tulino,&nbsp;M. Carballo,&nbsp;M. López Nigro,&nbsp;N. Magnani,&nbsp;P. Evelson,&nbsp;C. I. Casali,&nbsp;María del Carmen Fernández Tome","doi":"10.1002/biof.70018","DOIUrl":"https://doi.org/10.1002/biof.70018","url":null,"abstract":"<p>During the last three decades, resveratrol (RSV), a polyphenolic compound, has been proposed as a new panacea due to its all-potential beneficial actions. However, due to its hormetic properties, RSV has also been associated with toxic effects. In this work, we explored whether RSV affects osmoprotection and/or differentiation processes. Experiments were performed in Madin–Darby canine kidney (MDCK) cells subjected to high-NaCl hyperosmolar medium, a requisite for cell differentiation, in the absence or presence of RSV. Our results show that RSV does not affect osmoprotection but impedes epithelial cell differentiation by inducing oxidative stress, DNA damage, and impairing cell-cycle progression and motility. All these observations drive cells to stay in S phase and induce apoptosis. Our results warn about the possible harmful effects on tubular epithelial restitution processes or in postnatal kidney structures maturation when large quantities of this nutraceutical are ingested.</p>","PeriodicalId":8923,"journal":{"name":"BioFactors","volume":"51 2","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143845798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel peptide derived from Haematococcus pluvialis residue balanced lipid metabolism through NHR-49/PPARα and AAK-2/AMPK pathways in Caenorhabditis elegans","authors":"Liang Ke,&nbsp;Wanshi He,&nbsp;Chuyao Zong,&nbsp;Tiantian Wang,&nbsp;Jie Xiao,&nbsp;Yong Cao,&nbsp;Hang Xiao,&nbsp;Xiaojuan Liu","doi":"10.1002/biof.70017","DOIUrl":"https://doi.org/10.1002/biof.70017","url":null,"abstract":"<p>To explore the potential value of <i>Haematococcus pluvialis</i> residue after astaxanthin extraction, a novel peptide (HPp) was identified as a bioactive component. However, the possible lipid-lowering effect in vivo remains unclear. Thus, the classic model of <i>Caenorhabditis elegans</i> (<i>C. elegans</i>) was employed to evaluate the anti-obesity effects and underlying mechanism. The results showed that 100 μM HPp significantly reduced the overall fat and triglyceride contents, while also remarkably decreasing the lipid droplets size and promoting desaturation of C18:0 to C18:1n9. Subsequent analysis indicated that HPp increased energy expenditure and alleviated intestinal distension. Further molecular research revealed that HPp activated the gene expression of <i>fat-6</i>, <i>fat-7</i>, <i>nhr-49</i>, <i>acs-2</i>, <i>aak-2</i>, <i>atgl-1</i>. Notably, the lipid-lowering effects were abolished in <i>fat-6</i>, <i>fat-7</i>, <i>nhr-49</i> mutants, and further verified in GFP-tagged nematodes, indicating that HPp balanced lipid metabolism by activating NHR-49/PPARα and AAK-2/AMPK pathways in <i>C. elegans</i>. These findings highlight the high-value applications of marine microalgae.</p>","PeriodicalId":8923,"journal":{"name":"BioFactors","volume":"51 2","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143845799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}