Biophysical journal最新文献

{"title":"Physics of a super-fast viral jab.","authors":"Alex Mogilner","doi":"10.1016/j.bpj.2024.11.006","DOIUrl":"10.1016/j.bpj.2024.11.006","url":null,"abstract":"","PeriodicalId":8922,"journal":{"name":"Biophysical journal","volume":" ","pages":"1-2"},"PeriodicalIF":3.2,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11739864/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frequency-sensitive cell membrane dynamics under ultrasonic stimulation.","authors":"Bing Qi, Shaobao Liu","doi":"10.1016/j.bpj.2024.11.3321","DOIUrl":"10.1016/j.bpj.2024.11.3321","url":null,"abstract":"","PeriodicalId":8922,"journal":{"name":"Biophysical journal","volume":" ","pages":"10-11"},"PeriodicalIF":3.2,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11739865/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142754493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristic frequencies of localized stress relaxation in scaling-law rheology of living cells.","authors":"Jiu-Tao Hang, Huajian Gao, Guang-Kui Xu","doi":"10.1016/j.bpj.2024.11.015","DOIUrl":"10.1016/j.bpj.2024.11.015","url":null,"abstract":"<p><p>Living cells are known to exhibit power-law viscoelastic responses and localized stress relaxation behaviors in the frequency spectrum. However, the precise interplay between molecular-scale cytoskeletal dynamics and macroscale dynamical rheological responses remains elusive. Here, we propose a mechanism-based general theoretical model showing that cytoskeleton dissociation generates a peak in the loss modulus as a function of frequency, while the cytoplasmic viscosity promotes its recovery, producing a subsequent trough. We define two characteristic frequencies (ω_c1 and ω_c2) related to the dissociation rate of crosslinkers and the viscosity of the cytoplasm, where the loss modulus 1) exhibits peak and trough values for ω_c1<ω_c2 and 2) monotonically increases with frequency for ω_c1>ω_c2. Furthermore, the characteristic frequency ω_c1 exhibits a biphasic stress-dependent behavior, with a local minimum at sufficiently high stress due to the stress-dependent dissociation rate. Moreover, the characteristic frequency ω_c2 evolves with age, following a power-law relationship. The predictions of the dissociation-based multiscale theoretical mechanical model align well with experimental observations. Our model provides a comprehensive description of the dynamical viscoelastic behaviors of cells and cell-like materials.</p>","PeriodicalId":8922,"journal":{"name":"Biophysical journal","volume":" ","pages":"125-133"},"PeriodicalIF":3.2,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11739877/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive method to analyze single-cell vibrations.","authors":"Ali Al-Khaz'Aly, Salim Ghandorah, Jared J Topham, Nasir Osman, Taye Louie, Farshad Farshidfar, Matthias Amrein","doi":"10.1016/j.bpj.2024.11.003","DOIUrl":"10.1016/j.bpj.2024.11.003","url":null,"abstract":"<p><p>All living cells vibrate depending on metabolism. It has been hypothesized that vibrations are unique for a given phenotype and thereby suitable to diagnose cancer type and stage and to pre-assess the effectiveness of pharmaceutical treatments in real time. However, cells exhibit highly variable vibrational signals, can be subject to environmental noise, and may be challenging to differentiate, having so far limited the phenomenon's applicability. Here, we combined the sensitive method of force spectroscopy using optical tweezers with comprehensive statistical analysis. After data acquisition, the signal was decomposed into its spectral components via fast Fourier transform. Peaks were parameterized and subjected to principal-component analysis to perform an unbiased multivariate statistical evaluation. This method, which we term cell vibrational profiling (CVP), systematically assesses cellular vibrations. To validate the CVP technique, we conducted experiments on five U251 glioblastoma cells, using 8- to 10-μm polystyrene beads as a control for comparison. We collected raw data using optical tweezers, segmenting into 150+ 5-s intervals. Each segment was converted into power spectra representing a frequency resolution of 10,000 Hz for both cells and controls. U251 glioblastoma cells exhibited significant vibrations at 402.6, 1254.6, 1909.0, 2169.4, and 3462.8 Hz (p < 0.0001). This method was further verified with principal-component analysis modeling, which revealed that, in cell-cell comparisons using the selected frequencies, overlap frequently occurred, and clustering was difficult to discern. In contrast, comparison between cell-bead models showed that clustering was easily distinguishable. Our paper establishes CVP as an unbiased, comprehensive technique to analyze cell vibrations. This technique effectively differentiates between cell types and evaluates cellular responses to therapeutic interventions. Notably, CVP is a versatile, cell-agnostic technique requiring minimal sample preparation and no labeling or external interference. By enabling definitive phenotypic assessments, CVP holds promise as a diagnostic tool and could significantly enhance the evaluation of pharmaceutical treatments.</p>","PeriodicalId":8922,"journal":{"name":"Biophysical journal","volume":" ","pages":"77-92"},"PeriodicalIF":3.2,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11739874/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contact area and tissue growth dynamics shape synthetic juxtacrine signaling patterns.","authors":"Jonathan E Dawson, Abby Bryant, Breana Walton, Simran Bhikot, Shawn Macon, Amber Ajamu-Johnson, Trevor Jordan, Paul D Langridge, Abdul N Malmi-Kakkada","doi":"10.1016/j.bpj.2024.11.007","DOIUrl":"10.1016/j.bpj.2024.11.007","url":null,"abstract":"<p><p>Cell-cell communication through direct contact, or juxtacrine signaling, is important in development, disease, and many areas of physiology. Synthetic forms of juxtacrine signaling can be precisely controlled and operate orthogonally to native processes, making them a powerful reductionist tool with which to address fundamental questions in cell-cell communication in vivo. Here, we investigate how cell-cell contact length and tissue growth dynamics affect juxtacrine signal responses through implementing a custom synthetic gene circuit in Drosophila wing imaginal discs alongside mathematical modeling to determine synthetic Notch (synNotch) activation patterns. We find that the area of contact between cells largely determines the extent of synNotch activation, leading to the prediction that the shape of the interface between signal-sending and signal-receiving cells will impact the magnitude of the synNotch response. Notably, synNotch outputs form a graded spatial profile that extends several cell diameters from the signal source, providing evidence that the response to juxtacrine signals can persist in cells as they proliferate away from source cells, or that cells remain able to communicate directly over several cell diameters. Our model suggests that the former mechanism may be sufficient, since it predicts graded outputs without diffusion or long-range cell-cell communication. Overall, we identify that cell-cell contact area together with output synthesis and decay rates likely govern the pattern of synNotch outputs in both space and time during tissue growth, insights that may have broader implications for juxtacrine signaling in general.</p>","PeriodicalId":8922,"journal":{"name":"Biophysical journal","volume":" ","pages":"93-106"},"PeriodicalIF":3.2,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11739929/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vibrational signatures of living cells.","authors":"Oren Tchaicheeyan, Ramon Zaera Polo, Beth Mortimer, Ayelet Lesman","doi":"10.1016/j.bpj.2024.12.008","DOIUrl":"10.1016/j.bpj.2024.12.008","url":null,"abstract":"","PeriodicalId":8922,"journal":{"name":"Biophysical journal","volume":" ","pages":"12-14"},"PeriodicalIF":3.2,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11739867/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A physical model for M1-mediated influenza A virus assembly.","authors":"Julia Peukes, Serge Dmitrieff, François J Nédélec, John A G Briggs","doi":"10.1016/j.bpj.2024.11.016","DOIUrl":"10.1016/j.bpj.2024.11.016","url":null,"abstract":"<p><p>Influenza A virus particles assemble at the plasma membrane of infected cells. During assembly all components of the virus come together in a coordinated manner to deform the membrane into a protrusion eventually forming a new, membrane-enveloped virus. Here, we integrate recent molecular insights of this process, particularly concerning the structure of the matrix protein 1 (M1), within a theoretical framework describing the mechanics of virus assembly. Our model describes M1 polymerization and membrane protrusion formation, explaining why it is efficient for M1 to form long strands assembling into helices in filamentous virions. Eventually, we find how the architecture of M1 helices is controlled by physical properties of viral proteins and the host cell membrane. Finally, by considering the growth force and speed of viral filaments, we propose that the helical geometry of M1 strands might have evolved to optimize for fast and efficient virus assembly and growth.</p>","PeriodicalId":8922,"journal":{"name":"Biophysical journal","volume":" ","pages":"134-144"},"PeriodicalIF":3.2,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11739876/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tension makes the cell throw up","authors":"Hugo Lachuer","doi":"10.1016/j.bpj.2024.12.033","DOIUrl":"https://doi.org/10.1016/j.bpj.2024.12.033","url":null,"abstract":"","PeriodicalId":8922,"journal":{"name":"Biophysical journal","volume":"5 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Migrasome formation is initiated preferentially in tubular junctions by membrane tension.","authors":"Ben Zucker, Raviv Dharan, Dongju Wang, Li Yu, Raya Sorkin, Michael M Kozlov","doi":"10.1016/j.bpj.2024.12.029","DOIUrl":"10.1016/j.bpj.2024.12.029","url":null,"abstract":"<p><p>Migrasomes, the vesicle-like membrane microstructures, arise on the retraction fibers (RFs), the branched nanotubules pulled out of cell plasma membranes during cell migration and shaped by membrane tension. Migrasomes form in two steps: a local RF bulging is followed by a protein-dependent stabilization of the emerging spherical bulge. Here, we addressed theoretically and experimentally the previously unexplored mechanism of bulging of membrane tubular systems. We assumed that the bulging could be driven by increases in membrane tension and experimentally verified this hypothesis in live-cell and biomimetic systems. We exposed RF-generating live cells to a hypotonic medium, which produced water flows into the cells and a related increase in the membrane tension. We observed the formation of migrasome-like bulges with a preferential location in the RF branching sites. Next, we developed a biomimetic system of three membrane tubules pulled out of a giant plasma membrane vesicle (GPMV), connected by a junction, and subjected to pulling forces controlled by the GPMV membrane tension. An abrupt increase in the GPMV tension resulted in the generation of migrasome-like bulges mainly in the junctions. To understand the physical forces behind these observations, we considered theoretically the mechanical energy of a membrane system consisting of a three-way tubular junction with emerging tubular arms subjected to membrane tension. Substantiating our experimental observations, the energy minimization predicted a tension increase to drive the formation of membrane bulges, preferably in the junction site, independently of the way of the tension application. We generalized the model to derive universal criteria of bulging in branched membrane tubules.</p>","PeriodicalId":8922,"journal":{"name":"Biophysical journal","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142926386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Squishy things: Mechanophenotyping of actin networks in minimal cell models","authors":"Nadab Wubshet","doi":"10.1016/j.bpj.2024.12.035","DOIUrl":"https://doi.org/10.1016/j.bpj.2024.12.035","url":null,"abstract":"","PeriodicalId":8922,"journal":{"name":"Biophysical journal","volume":"49 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}