Biophysical journal最新文献

The Dual Nature of Body-Axis Formation in Hydra Regeneration: Polarity-Morphology Concurrency. 水螅再生中体-轴形成的双重性：极性-形态并发性。

IF 3.1 3区生物学

Biophysical journal Pub Date : 2026-05-08 DOI: 10.1016/j.bpj.2026.05.009

Oded Agam, Erez Braun

{"title":"The Dual Nature of Body-Axis Formation in Hydra Regeneration: Polarity-Morphology Concurrency.","authors":"Oded Agam, Erez Braun","doi":"10.1016/j.bpj.2026.05.009","DOIUrl":"https://doi.org/10.1016/j.bpj.2026.05.009","url":null,"abstract":"The formation of a body-axis is central to animal development and involves both polarity and morphology. While polarity is traditionally associated with biochemical patterning, the morphological aspect of axis formation remains elusive. In regenerating Hydra tissues, we find that morphological evolution in all tissue samples depends on inherited positional information from the donor's axis, and a foot precursor emerges early in the process. From the onset of regeneration, the Ca2+ excitations that drive actomyosin forces for tissue reshaping follow a gradient aligned with the inferred head-foot polarity direction. We conclude that polarity and morphological axis progression occur concurrently through interlinked processes. In this progression, the early and reproducible emergence of a foot precursor provides a robust morphological marker of axis formation, a role often discussed primarily in the context of the head organizer. A toy model accounts for the observed regeneration dynamics and illustrates the mechanochemical integration of polarity and morphogenesis. We expect the insights from Hydra to be relevant to broader developmental systems.","PeriodicalId":8922,"journal":{"name":"Biophysical journal","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147855781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The cytoskeleton as an active regulator of membrane-protein condensation: a mesoscale simulation study. 细胞骨架作为膜-蛋白缩聚的主动调节剂：中尺度模拟研究。

IF 3.1 3区生物学

Biophysical journal Pub Date : 2026-05-07 DOI: 10.1016/j.bpj.2026.05.010

Yukang Zhang, Dechang Li

引用次数: 0

Active deformation in the basal organ of Corti in gerbil. 沙鼠Corti基底器官的主动变形。

IF 3.1 3区生物学

Biophysical journal Pub Date : 2026-05-07 DOI: 10.1016/j.bpj.2026.04.037

Kaitlyn H Wong, C Elliott Strimbu, Elizabeth S Olson

{"title":"Active deformation in the basal organ of Corti in gerbil.","authors":"Kaitlyn H Wong, C Elliott Strimbu, Elizabeth S Olson","doi":"10.1016/j.bpj.2026.04.037","DOIUrl":"https://doi.org/10.1016/j.bpj.2026.04.037","url":null,"abstract":"Optical coherence tomography (OCT) has allowed in vivo recording of sound-induced vibrations of different regions within the organ of Corti complex (OCC), including the basilar membrane (BM), outer hair cell/Deiters cell (OHC/DC) region, and reticular lamina (RL). In the hook region of the gerbil cochlea, where measurements can be made with a predominantly transverse optical axis, the three regions have different and characteristic motion responses: The OHC/DC region has greater motions than the other two regions at frequencies below the best frequency (sub-BF); the RL region typically has the greatest BF peak and smallest sub-BF motion. The phase of the OHC/DC-region motion increasingly lags BM motion phase as frequency increases; the RL-region motion phase leads BM, but with a relatively small value. All three regions are compressively nonlinear in the BF peak, but only the OHC/DC region shows sub-BF compressive nonlinearity. Here we describe the strains that arise when the motion transitions between these regions. Large strains (deformations) were observed in the OHC body close to the RL. A region of large strain can be as short as a single 2.7 μm measurement pixel, or can extend over several pixels, with extensive strains appearing more often at 70 than at 50 dB SPL. Beyond this RL region of large strain, over a distance that can exceed 20 μm, the OHC/DC region displayed nearly spatially-unvarying motion - this region vibrated as a relatively rigid body. And beyond this, at sub-BF frequencies another region of high strain was present, indicating stretching/compressing, or bowing/tilting of the DC microtubular stalk.","PeriodicalId":8922,"journal":{"name":"Biophysical journal","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147855788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellular nematics speeding up calcium propagation. 细胞向列线加速钙的繁殖。

IF 3.1 3区生物学

Biophysical journal Pub Date : 2026-05-07 DOI: 10.1016/j.bpj.2026.05.004

Aashrith Saraswathibhatla

引用次数: 0

Phosphorylation-Dependent Structure and Dynamics of Caveolin-1 8S Complex. 小洞蛋白- 18s复合物的磷酸化依赖性结构和动力学。

IF 3.1 3区生物学

Biophysical journal Pub Date : 2026-05-06 DOI: 10.1016/j.bpj.2026.04.033

Ukesh Karki, Sadeq Shabani, Prabin Dahal, Cathy Padilla, Joshua Hutcheson, Prem Chapagain

{"title":"Phosphorylation-Dependent Structure and Dynamics of Caveolin-1 8S Complex.","authors":"Ukesh Karki, Sadeq Shabani, Prabin Dahal, Cathy Padilla, Joshua Hutcheson, Prem Chapagain","doi":"10.1016/j.bpj.2026.04.033","DOIUrl":"https://doi.org/10.1016/j.bpj.2026.04.033","url":null,"abstract":"Caveolin-1 (Cav-1) is a membrane-associated scaffolding protein essential for lipid regulation, cellular signaling, and caveolae formation. Phosphorylation at tyrosine 14 (Y14) of Cav-1 plays a pivotal role in modulating its functional dynamics, but the structural consequences of this modification remain unexplored. This site belongs to the N-terminal tail, which is unresolved in the recent CryoEM structures of the Cav-1 8S complexes. In this work, we used AlphaFold 3 to generate a full-length model of the human Cav-1 8S complex and its phosphorylated form (pCav-1) and performed molecular dynamics simulations of both complexes embedded in the plasma membrane. Inclusion of the N-terminal tail in the AF3-predicted models significantly enhances protein-membrane interactions, highlighting the membrane-binding role of the N-terminal tail. Our results show that the Y14 phosphorylation induces significant conformational changes to the N-terminal tail structure with enhanced inter-protomer hydrogen bonding resulting in an altered conformational state. The results provide mechanistic insights into how phosphorylation may act as a molecular switch that regulates Cav-1's structural behavior, membrane affinity, and caveolae biogenesis.","PeriodicalId":8922,"journal":{"name":"Biophysical journal","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147832884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gating shift to an ohmic mode of TREK-1 channel induced by docosahexaenoic acid. 二十二碳六烯酸诱导TREK-1通道向欧姆模式的门控移位。

IF 3.1 3区生物学

Biophysical journal Pub Date : 2026-05-06 DOI: 10.1016/j.bpj.2026.05.005

Emilie Bechard, Jean-Yves Le Guennec, Marie Demion

{"title":"Gating shift to an ohmic mode of TREK-1 channel induced by docosahexaenoic acid.","authors":"Emilie Bechard, Jean-Yves Le Guennec, Marie Demion","doi":"10.1016/j.bpj.2026.05.005","DOIUrl":"https://doi.org/10.1016/j.bpj.2026.05.005","url":null,"abstract":"TREK-1 channel is a polymodulated K2P channel that responds to a wide range of physical and chemical stimuli, enabling it to contribute to the regulation of cellular excitability. This member of the TREK subfamily exhibits an outward rectification resulting from a voltage-dependent gating, which can be reversibly converted into an ohmic leak mode by both synthetic and natural modulators. The present study aimed to investigate the gating mechanism in response to docosahexaenoic acid (DHA), using transient transfections of either WT TREK-1 channel or its G171D variant at the TM2.6 position in HEK 293T cells. We reported that DHA enhances WT TREK-1 current by increasing the proportion of channels in a conductive state across the entire range of Vm, effectively shifting TREK-1 into an ohmic mode. Furthermore, we observed that disrupting the C-type gate function blunted DHA-induced activation, suggesting that DHA acts through a stabilization of the selectivity filter that may involve the TM2. Additionally, we observed considerable variability in the initial activity of TREK-1 under control conditions, which accounts for the heterogeneous effects of DHA among cells. These findings highlight the complex gating mechanism of TREK-1 and reveal a dynamic mechanism by which DHA, and likely other PUFAs, modulate membrane potential and influence cellular excitability.","PeriodicalId":8922,"journal":{"name":"Biophysical journal","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147832852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proton Hopping and Secondary Gating Reveal a Universal Transport Mechanism in a Minimal Bacterial Transporter EmrE. 质子跳跃和二次门控揭示了一个最小细菌转运体EmrE的普遍转运机制。

IF 3.1 3区生物学

Biophysical journal Pub Date : 2026-05-06 DOI: 10.1016/j.bpj.2026.04.039

Da Teng, Merissa Brousseau, Jong Ho Choi, Katherine A Henzler-Wildman, Gregory A Voth

{"title":"Proton Hopping and Secondary Gating Reveal a Universal Transport Mechanism in a Minimal Bacterial Transporter EmrE.","authors":"Da Teng, Merissa Brousseau, Jong Ho Choi, Katherine A Henzler-Wildman, Gregory A Voth","doi":"10.1016/j.bpj.2026.04.039","DOIUrl":"https://doi.org/10.1016/j.bpj.2026.04.039","url":null,"abstract":"The alternating access model has been widely applied to help understand many secondary transporters, but there are emerging exceptions that question the universal validity of such a theory. EmrE is a Small Multidrug Resistant (SMR) Transporter that utilizes the proton gradient across the membrane to efflux drug molecules from the cell. Recent experimental evidence has suggested that EmrE does not meet the conditions required for the alternating access model but still achieves coupled transport. This, consequently, suggests the need for a universal exchange model to explain the coupled transport. In this paper, through a combined computational and experimental approach, we provide key evidence that the transport cycle of EmrE follows the universal exchange model. First, we show that a proton can move between the two drug/proton co-binding glutamic acid residues (E14s) in proximity to one another (\"proton hopping\") at a rate faster than alternating access, which is a key step in the universal exchange model. Furthermore, we show that when both sites are bound with protons, two peripheral residues E25 and T56 will coordinate to occlude EmrE to prevent proton leak. The proposed model rectifies outstanding differences in the current mechanistic understanding of promiscuous SMR transporters and defines a means of maintaining a high degree of coupling with increased substrate promiscuity.","PeriodicalId":8922,"journal":{"name":"Biophysical journal","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147832858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular-level interaction of dopamine with lipids studied by sum frequency generation spectroscopy. 和频产生光谱法研究多巴胺与脂质的分子水平相互作用。

IF 3.1 3区生物学

Biophysical journal Pub Date : 2026-05-05 DOI: 10.1016/j.bpj.2026.05.002

Haicui Dong, Cunsheng Wei, Xiaoqing Lian, Jimeng Yang, Qin Tao, Pengcheng Hu

{"title":"Molecular-level interaction of dopamine with lipids studied by sum frequency generation spectroscopy.","authors":"Haicui Dong, Cunsheng Wei, Xiaoqing Lian, Jimeng Yang, Qin Tao, Pengcheng Hu","doi":"10.1016/j.bpj.2026.05.002","DOIUrl":"https://doi.org/10.1016/j.bpj.2026.05.002","url":null,"abstract":"Dopamine is a clinically essential pharmaceutical agent extensively used in cardiology and emergency medicine. During the drug treatment, interactions between dopamine and lipid membranes are unavoidable. However, their molecular-level interaction remains insufficiently understood. In this research, Sum frequency generation (SFG) spectroscopy was utilized to investigate the molecular-level interaction of dopamine with the negatively charged phosphatidylglycerol (PG) lipid bilayer, in real time and in situ. Our results reveal that both the outer and inner PG leaflets remain unaffected at a low dopamine concentration of 1.0 mM. In contrast, at 10 mM dopamine, sight perturbations to the outer PG leaflet were observed due to the hydrogen bonds and electrostatic interactions formed between them. The average tilt angles of the CD3 groups were calculated to be 33° and 38° before and after the addition of 10 mM dopamine, respectively. To the best of our understanding, this work experimentally quantified the dopamine-induced orientation variations of lipid molecules in a bilayer for the first time. These findings provide molecular-level insights into dopamine-membrane interactions, offering potential guidance for the development of new cardiovascular therapeutics involving dopamine-related membrane processes, such as treatments for hypotension, shock, and heart failure.","PeriodicalId":8922,"journal":{"name":"Biophysical journal","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147832866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chaperone proteins protect against desmin fragment amyloid aggregation. 伴侣蛋白可以防止消蛋白片段淀粉样蛋白聚集。

IF 3.1 3区生物学

Biophysical journal Pub Date : 2026-05-05 DOI: 10.1016/j.bpj.2026.04.038

Erin M Mulhearn, Ariel M Alperstein

引用次数: 0

Subdomains of Endophilin Can Drive Membrane Remodeling and Facilitate Controlled Membrane Scission. 嗜内啡肽的子结构域可以驱动膜重塑和促进可控的膜断裂。

IF 3.1 3区生物学

Biophysical journal Pub Date : 2026-05-05 DOI: 10.1016/j.bpj.2026.05.003

Jeriann R Beiter, Feng-Ching Tsai, Patricia Bassereau, Gregory A Voth

引用次数: 0