Chimerism最新文献_第6页

Chimerism Volume 4, Issue 1 Table of Contents 嵌合第4卷，第1期目录

Chimerism Pub Date : 2013-01-01 DOI: 10.4161/chim.24515

引用次数: 0

Reconsidering the bio-detection of tolerance in renal transplantation. 肾移植耐受生物检测的再思考。

Chimerism Pub Date : 2013-01-01 DOI: 10.4161/chim.23347

Faouzi Braza, Jean Paul Soulillou, Sophie Brouard

引用次数: 2

We are all born as microchimera. 我们都是天生的微型嵌合体。

Chimerism Pub Date : 2013-01-01 Epub Date: 2012-12-21 DOI: 10.4161/chim.23348

Miranda P Dierselhuis, Els Goulmy

引用次数: 12

Validation of chimerism in pediatric recipients of allogeneic hematopoietic stem cell transplantation (HSCT) a comparison between two methods: real-time PCR (qPCR) vs. variable number tandem repeats PCR (VNTR PCR). 验证儿童同种异体造血干细胞移植(HSCT)受体嵌合:实时PCR (qPCR)和可变数串联重复PCR (VNTR PCR)两种方法的比较。

Chimerism Pub Date : 2013-01-01 Epub Date: 2012-12-13 DOI: 10.4161/chim.23158

Morris Kletzel, Wei Huang, Marie Olszewski, Sana Khan

{"title":"Validation of chimerism in pediatric recipients of allogeneic hematopoietic stem cell transplantation (HSCT) a comparison between two methods: real-time PCR (qPCR) vs. variable number tandem repeats PCR (VNTR PCR).","authors":"Morris Kletzel, Wei Huang, Marie Olszewski, Sana Khan","doi":"10.4161/chim.23158","DOIUrl":"https://doi.org/10.4161/chim.23158","url":null,"abstract":"Post-hematopoietic stem cell transplantation (HSCT) chimerism monitoring is important to assess relapse and therapeutic intervention. The purpose of our study is to compare two methods variable number tandem repeats (VNTR) vs. quantitative real- time polymerase chain reaction (qPCR) in terms of determining chimerism. 127 (peripheral blood n=112, bone marrow n=15) samples were simultaneously tested by VNTR using APO-B, D1S80, D1S111, D17S30, gene loci SRY and ZP3 and qPCR using 34 assays (CA001-CA034) that are designed to a bi-allelic insertion/deletion (indel) polymorphism in the human genome. Samples were separated in three subsets: total WBC, T-cell and Myeloid cells. Extraction of DNA was performed then quantified. We analyzed column statistics, paired t-test and regression analysis for both methods. There was complete correlation between the two methods. The simplicity and rapidity of the test results from the qPCR method is more efficient and accurate to assess chimerism.","PeriodicalId":89114,"journal":{"name":"Chimerism","volume":"4 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/chim.23158","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31118234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26

Molecular chimerism in IgE-mediated allergy: B-and T-cell tolerance toward highly immunogenic exogenous antigens. IgE 介导的过敏症中的分子嵌合体：B 细胞和 T 细胞对高免疫原性外来抗原的耐受性。

Chimerism Pub Date : 2013-01-01 DOI: 10.4161/chim.24071

Ulrike Baranyi, Rudolf Valenta, Thomas Wekerle

{"title":"Molecular chimerism in IgE-mediated allergy: B-and T-cell tolerance toward highly immunogenic exogenous antigens.","authors":"Ulrike Baranyi, Rudolf Valenta, Thomas Wekerle","doi":"10.4161/chim.24071","DOIUrl":"10.4161/chim.24071","url":null,"abstract":"Specific immunotherapy is the only curative treatment currently available for IgE-mediated allergy and preventive strategies are lacking altogether. We have recently reported that molecular chimerism induces durable tolerance in experimental models of allergy, thus potentially providing a new approach for the treatment and prevention of allergic diseases. Molecular chimerism is a gene-therapy approach for tolerance induction toward defined disease-causing antigens. In proof-of-concept studies, we introduced a clinically relevant grass pollen allergen into hematopoietic stem cells and transplanted those modified cells into preconditioned syngeneic mice. Long-lasting and robust tolerance toward the allergen was achieved. In our most recent studies published in Clinical and Experimental Allergy we demonstrated that milder, non-myeloablative conditioning is sufficient to induce tolerance. Our results revealed that, in contrast to other rodent models of chimerism, persistent microchimerism suffices to induce lasting tolerance at the T cell, B cell and effector cell levels in IgE-mediated allergy. This article addendum provides a summary of the recent paper and its implications.","PeriodicalId":89114,"journal":{"name":"Chimerism","volume":"4 1","pages":"29-31"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654736/pdf/chim-4-29.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31461354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Haploidentical hematopoietic stem cell transplantation without in vitro T-cell-depletion for the treatment of hematologic diseases. 无体外t细胞耗竭的单倍体造血干细胞移植治疗血液病。

Chimerism Pub Date : 2013-01-01 DOI: 10.4161/chim.24070

Xiao-Jun Huang

{"title":"Haploidentical hematopoietic stem cell transplantation without in vitro T-cell-depletion for the treatment of hematologic diseases.","authors":"Xiao-Jun Huang","doi":"10.4161/chim.24070","DOIUrl":"https://doi.org/10.4161/chim.24070","url":null,"abstract":"Haploidentical hematopoietic stem cell transplantation (HSCT) has been accepted worldwide as an alternative treatment for patients with hematologic diseases who do not have a human leukocyte antigen (HLA) identical sibling donor or who require urgent transplantation. The results from our nine-year experience showed that granulocyte colony-stimulating factor (G-CSF) primed bone marrow (G-BM) combined with peripheral blood grafts (G-PB) from haploidentical donors, without in vitro T cell depletion (TCD), is a reliable source of stem cells for transplantation to cure acute leukemia and chronic myeloid leukemia. Recent findings confirmed that unmanipulated haploidentical HSCT is a promising protocol that can be successfully extended to treat intermediate and high-risk myelodysplastic syndrome and severe aplastic anemia. Recent observations suggest the association of improved immune recovery with better transplant outcomes after haploidentical HSCT. Chronic graft-vs.-host-disease severity strongly correlates with negative impacts on patients' health-related quality of life, suggesting that it should be successfully controlled. ","PeriodicalId":89114,"journal":{"name":"Chimerism","volume":"4 1","pages":"26-8"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/chim.24070","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31254515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Toward minimal conditioning protocols for allogeneic chimerism in tolerance resistant recipients. 对耐受耐受受体同种异体嵌合的最小调理方案。

Chimerism Pub Date : 2013-01-01 DOI: 10.4161/chim.23350

David P Al-Adra, Colin C Anderson

引用次数: 2

Combined treatment with regulatory T cells and vascularized bone marrow transplantation creates mixed chimerism and induces donor-specific tolerance to vascularized composite allografts without cytoreductive conditioning. 调节性T细胞和血管化骨髓移植联合治疗可产生混合嵌合，并诱导供体特异性耐受血管化复合同种异体移植物，而无需细胞减少条件。

Chimerism Pub Date : 2013-01-01 DOI: 10.4161/chim.23349

Jeng-Yee Lin, Feng-Chou Tsai, Christopher Glenn Wallace, Wei-Chao Huang, Fu-Chan Wei, Shuen-Kuei Liao

引用次数: 3

Increased maternal microchimerism after open fetal surgery. 开放胎儿手术后母体微嵌合增加。

Chimerism Pub Date : 2012-07-01 DOI: 10.4161/chim.22277

Payam Saadai, Tippi C MacKenzie

引用次数: 5

The natural history of fetal cells in postpartum murine maternal lung and bone marrow: a two-stage phenomenon. 产后小鼠母体肺和骨髓中胎儿细胞的自然史:一个两阶段的现象。

Chimerism Pub Date : 2012-07-01 DOI: 10.4161/chim.22769

Stephanie Pritchard, Inga Peter, Kirby L Johnson, Diana W Bianchi

{"title":"The natural history of fetal cells in postpartum murine maternal lung and bone marrow: a two-stage phenomenon.","authors":"Stephanie Pritchard, Inga Peter, Kirby L Johnson, Diana W Bianchi","doi":"10.4161/chim.22769","DOIUrl":"https://doi.org/10.4161/chim.22769","url":null,"abstract":"During pregnancy, fetal cells cross into the maternal organs where they reside postpartum. Evidence from multiple laboratories suggests that these microchimeric fetal cells contribute to maternal tissue repair after injury. In mouse models, most injury experiments are performed during pregnancy; however, in a clinical setting most injuries or diseases occur postpartum. Therefore, experiments using animal models should be designed to address questions in the time period following delivery. In order to provide a baseline for such experiments, we analyzed the natural history of fetal cells in the postpartum maternal organs. Female C57BL/6J mice were mated to males homozygous for the enhanced green fluorescent protein gene. Fetal cells in the maternal lungs and bone marrow were identified by their green fluorescence using in a high-speed flow cytometer and their counts were compared between the lung and bone marrow. Spearman correlation analysis was used to identify relationships between the duration of time postpartum and the cell counts and ratio of live and dead cells. Our results show that fetal cells persist in these organs until at least three months postpartum in healthy female mice. We show a two-stage decline, with an initial two and a half-week rapid clearance followed by a trend of gradual decrease. Additionally, an increase in the ratio of live to dead cells within the lung over time suggests that these cells may replicate in vivo. The results presented here will inform the design of future experiments and may have implications for women's health.","PeriodicalId":89114,"journal":{"name":"Chimerism","volume":"3 3","pages":"59-64"},"PeriodicalIF":0.0,"publicationDate":"2012-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/chim.22769","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31026268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6