Bioengineered bugs最新文献_第9页

Molecular responses of Escherichia coli caused by heat stress and recombinant protein production during temperature induction. 高温诱导下大肠杆菌热应激的分子响应及重组蛋白的产生。

Bioengineered bugs Pub Date : 2011-03-01 DOI: 10.4161/bbug.2.2.14316

Norma A Valdez-Cruz, Octavio T Ramírez, Mauricio A Trujillo-Roldán

引用次数: 19

Time it was, and what a time it was... 是时候了，是多么美好的时刻啊……

Bioengineered bugs Pub Date : 2011-01-01 DOI: 10.4161/bbug.2.1.14315

Roy D Sleator

引用次数: 24

Proteomics in the microbial sciences. 微生物科学中的蛋白质组学。

Bioengineered bugs Pub Date : 2011-01-01 DOI: 10.4161/bbug.2.1.14413

Ciaren Graham, Geoff McMullan, Robert L J Graham

引用次数: 30

Escherichia coli K5 heparosan fermentation and improvement by genetic engineering. 大肠杆菌K5肝素聚糖的发酵及基因工程改良。

Bioengineered bugs Pub Date : 2011-01-01 DOI: 10.4161/bbug.2.1.14201

Zhenyu Wang, Jonathan S Dordick, Robert J Linhardt

引用次数: 50

Parenteral vaccination of mammalian livestock with Newcastle disease virus-based vector vaccines offers optimal efficacy and safety. 以新城疫病毒为基础的载体疫苗对哺乳动物进行肠外接种具有最佳的有效性和安全性。

Bioengineered bugs Pub Date : 2011-01-01 DOI: 10.4161/bbug.2.1.13349

Michiel M Harmsen, Adriaan F G Antonis, Rob J M Moormann, Jeroen Kortekaas

{"title":"Parenteral vaccination of mammalian livestock with Newcastle disease virus-based vector vaccines offers optimal efficacy and safety.","authors":"Michiel M Harmsen, Adriaan F G Antonis, Rob J M Moormann, Jeroen Kortekaas","doi":"10.4161/bbug.2.1.13349","DOIUrl":"https://doi.org/10.4161/bbug.2.1.13349","url":null,"abstract":"Newcastle disease virus (NDV) is an avian virus that is being evaluated as a vaccine vector for the delivery of foreign genes in mammals. The use of NDV as a vaccine vector in these species offers two major advantages. First, NDV is highly attenuated in mammals, rendering its use inherently safe. Second, mammals lack pre-existing NDV immunity, which minimizes the risk of vaccination failure. NDV-vector vaccines are generally administered to mammals via the respiratory route. We recently showed that intramuscular vaccination with NDV-based Rift Valley fever virus (RVFV) vaccines provides complete protection in mice and induces neutralizing antibodies in sheep and cattle, the main target species of RVFV. Here, we discuss the use of NDV as a vaccine vector for applications in mammalian livestock with an emphasis on the vaccination route. We also report the results of novel experiments that underscore our notion that vaccination via a parenteral route is more effective than immunization via the respiratory route.","PeriodicalId":89091,"journal":{"name":"Bioengineered bugs","volume":"2 1","pages":"58-62"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bbug.2.1.13349","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29910145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

The microalga Chlamydomonas reinhardtii as a platform for the production of human protein therapeutics. 微藻莱茵衣藻作为生产人类蛋白质治疗剂的平台。

Bioengineered bugs Pub Date : 2011-01-01 DOI: 10.4161/bbug.2.1.13423

Beth A Rasala, Stephen P Mayfield

{"title":"The microalga Chlamydomonas reinhardtii as a platform for the production of human protein therapeutics.","authors":"Beth A Rasala, Stephen P Mayfield","doi":"10.4161/bbug.2.1.13423","DOIUrl":"https://doi.org/10.4161/bbug.2.1.13423","url":null,"abstract":"Microalgae are a diverse group of eukaryotic photosynthetic microorganisms. While microalgae play a crucial role in global carbon fixation and oxygen evolution, these organisms have recently gained much attention for their potential role in biotechnological and industrial applications, such as the production of biofuels. We investigated the potential of the microalga Chlamydomonas reinhardtii to be a platform for the production of human therapeutic proteins. C. reinhardtii is a unicellular freshwater green alga that has served as a popular model alga for physiological, molecular, biochemical and genetic studies. As such, the molecular toolkit for this microorganism is highly developed, including well-established methods for genetic transformation and recombinant gene expression. We transformed the chloroplast genome of C. reinhardtii with seven unrelated genes encoding for current or potential human therapeutic proteins and found that four of these genes supported protein accumulation to levels that are sufficient for commercial production. Furthermore, the algal-produced proteins were bioactive. Thus, the microalga C. reinhardtii has the potential to be a robust platform for human therapeutic protein production.","PeriodicalId":89091,"journal":{"name":"Bioengineered bugs","volume":"2 1","pages":"50-4"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bbug.2.1.13423","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29910146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 106

Transformation of the mycorrhizal fungus Laccaria bicolor using Agrobacterium tumefaciens. 农杆菌转化菌根真菌双色拉卡菌。

Bioengineered bugs Pub Date : 2011-01-01 DOI: 10.4161/bbug.2.1.14394

Minna J Kemppainen, Alejandro G Pardo

引用次数: 16

Development of a broad-host-range phage cocktail for biocontrol. 用于生物防治的宽宿主噬菌体鸡尾酒的研制。

Bioengineered bugs Pub Date : 2011-01-01 DOI: 10.4161/bbug.2.1.13657

David Kelly, Olivia McAuliffe, R Paul Ross, Jim O'Mahony, Aidan Coffey

{"title":"Development of a broad-host-range phage cocktail for biocontrol.","authors":"David Kelly, Olivia McAuliffe, R Paul Ross, Jim O'Mahony, Aidan Coffey","doi":"10.4161/bbug.2.1.13657","DOIUrl":"https://doi.org/10.4161/bbug.2.1.13657","url":null,"abstract":"The aims of this study were to investigate the incidence of different resistance mechanisms to phage K in a bank of Irish Staph aureus hospital strains; and to develop a broad host-range phage cocktail with enhanced lytic activity against those strains which were previously phage resistant. A bank of 180 Staph aureus strains, which included all the sequence types currently in existence in Ireland, were tested for sensitivity to phage K. Twenty nine strains were identified, which did not permit plaque formation. The phage resistance systems in the 29 strain were investigated and it was found that restriction modification (r-m) was evident in 24, an adsorption inhibition mechanism was evident in three, while two were resistant by an unidentified mechanism. Seventeen modified derivatives of phage K were developed which could circumvent all the r-m systems. Nevertheless, six of the modified phage were considered superior in terms of their individual host ranges. These six were pooled as a cocktail with phage K, which then lysed 24 of the 29 resistant strains (97.2% of the entire staphylococcal bank). In conclusion, phage resistant systems affecting phage K are common in Staph. aureus but it is possible to significantly broaden the host-range of this phage for biocontrol applications.","PeriodicalId":89091,"journal":{"name":"Bioengineered bugs","volume":"2 1","pages":"31-7"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bbug.2.1.13657","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29910141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 57

Efficient E. coli expression systems for the production of recombinant β-mannanases and other bacterial extracellular enzymes. 高效大肠杆菌表达系统生产重组β-甘露聚糖酶和其他细菌胞外酶。

Bioengineered bugs Pub Date : 2011-01-01 DOI: 10.4161/bbug.2.1.13419

Montarop Yamabhai, Bancha Buranabanyat, Nanthnit Jaruseranee, Chomphunuch Songsiriritthigul

{"title":"Efficient E. coli expression systems for the production of recombinant β-mannanases and other bacterial extracellular enzymes.","authors":"Montarop Yamabhai, Bancha Buranabanyat, Nanthnit Jaruseranee, Chomphunuch Songsiriritthigul","doi":"10.4161/bbug.2.1.13419","DOIUrl":"https://doi.org/10.4161/bbug.2.1.13419","url":null,"abstract":"Two Escherichia coli expression systems based on T7 RNA polymerase promoter (pET system) and tac promoter (pFLAG system) have been used for the production and secretion of recombinant β-mannanases from Bacillus sp. Both E. coli OmpA signal peptide and native Bacillus signal peptide could be used efficiently for the secretion of recombinant enzymes into periplasmic space and culture media. The genes could be induced for over-expression with 0.1-1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG) when the OD 600 of the culture broth reached 0.6-1.5. The recombinant enzymes could be harvested from whole cell lysate, perimplasmic extract, or culture broth after induction for 4-20 hours. Since the enzyme is C-terminally tagged with hexahistidine, the recombinant enzymes could be conveniently purified to apparent homogeneity by one-step immobilized-metal affinity chromatography (IMAC) using Ni-NTA resins. The characteristics of purified recombinant β-mannanases from B. licheniformis and B. subtilis, which share 78% amino acid identity, are slightly different. These systems should be applicable for the production of various recombinant bacterial extracellular enzymes.","PeriodicalId":89091,"journal":{"name":"Bioengineered bugs","volume":"2 1","pages":"45-9"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bbug.2.1.13419","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29910143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Bacterial luciferase reporters: the Swiss army knife of molecular biology. 细菌荧光素酶报告者:分子生物学的瑞士军刀。

Bioengineered bugs Pub Date : 2011-01-01 DOI: 10.4161/bbug.2.1.13566

Mark S Waidmann, Fenja S Bleichrodt, Tanja Laslo, Christian U Riedel

引用次数: 61