Bioengineered bugs最新文献_第7页

The future of industrial antibiotic production: From random mutagenesis to synthetic biology 工业抗生素生产的未来:从随机诱变到合成生物学

Bioengineered bugs Pub Date : 2011-07-01 DOI: 10.4161/bbug.2.4.16114

M. Medema, M. Alam, R. Breitling, E. Takano

引用次数: 38

Cell immobilization studies using a cellulose-binding domain fused to PrtP in Lactococcus lactis. 在乳酸乳球菌中使用纤维素结合结构域与PrtP融合的细胞固定化研究。

Bioengineered bugs Pub Date : 2011-05-01 DOI: 10.4161/bbug.2.3.15348

Shanna Liu, Kari Kylä-Nikkilä, Per E J Saris

引用次数: 7

Recombinant adeno-associated virus expressing a modified transcription factor triggered by the hepatitis C viral protease NS3/4a. 表达由丙型肝炎病毒蛋白酶NS3/4a触发的修饰转录因子的重组腺相关病毒。

Bioengineered bugs Pub Date : 2011-05-01 DOI: 10.4161/bbug.2.3.15449

Chul Hyun Joo

引用次数: 1

Engineered cyanobacteria: teaching an old bug new tricks. 工程蓝藻:教老虫子新把戏。

Bioengineered bugs Pub Date : 2011-05-01 DOI: 10.4161/bbug.2.3.15285

Anne M Ruffing

{"title":"Engineered cyanobacteria: teaching an old bug new tricks.","authors":"Anne M Ruffing","doi":"10.4161/bbug.2.3.15285","DOIUrl":"https://doi.org/10.4161/bbug.2.3.15285","url":null,"abstract":"Cyanobacteria have played an important role in the development of the Earth and have long been studied as model organisms for photosynthesis and the circadian rhythm. Recent developments have led to increased interest in the use of engineered cyanobacteria for the production of protein and chemical products. This review highlights the genetic tools and strategies for manipulation of cyanobacteria as well as previous accomplishments in the development of engineered cyanobacteria for applied use. Particular attention is given to the engineering of cyanobacteria for biofuel production, including both hydrocarbon and hydrogen fuels. Genetic engineering efforts to enhance cyanobacterial fitness are reviewed with an emphasis on physiological improvements for large-scale production. Lastly, a future outlook on engineered cyanobacteria is presented, highlighting the future areas of focus and technical challenges in this field. With the uncertainty of future energy security, it is an exciting time in applied cyanobacterial research, but we must take the time to learn from these past accomplishments before we can capitalize on the potential of these photosynthetic microorganisms. ","PeriodicalId":89091,"journal":{"name":"Bioengineered bugs","volume":"2 3","pages":"136-49"},"PeriodicalIF":0.0,"publicationDate":"2011-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bbug.2.3.15285","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29910000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 107

Vaccine and adjuvant design for emerging viruses: mutations, deletions, segments and signaling. 新出现病毒的疫苗和佐剂设计：突变、缺失、片段和信号传导。

Bioengineered bugs Pub Date : 2011-05-01 DOI: 10.4161/bbug.2.3.15367

Gavin C Bowick, Alexander J McAuley

{"title":"Vaccine and adjuvant design for emerging viruses: mutations, deletions, segments and signaling.","authors":"Gavin C Bowick, Alexander J McAuley","doi":"10.4161/bbug.2.3.15367","DOIUrl":"10.4161/bbug.2.3.15367","url":null,"abstract":"Vaccination is currently the most effective strategy to medically control viral diseases. However, developing vaccines is a long and expensive process, and traditional methods, such as attenuating wild-type viruses by serial passage, may not be suitable for all viruses and may lead to vaccine safety considerations, particularly in the case of the vaccination of particular patient groups, such as the immunocompromised and the elderly. In particular, developing vaccines against emerging viral pathogens adds a further level of complexity, as they may only be administered to small groups of people or only in response to a specific event or threat, limiting our ability to study and evaluate responses. In this commentary, we discuss how novel techniques may be used to engineer a new generation of vaccine candidates as we move toward a more targeted vaccine design strategy, driven by our understanding of the mechanisms of viral pathogenesis, attenuation and the signaling events which are required to develop a lasting, protective immunity. We will also briefly discuss the potential future role of vaccine adjuvants, which could be used to bridge the gap between vaccine safety, and lasting immunity from a single vaccination. ","PeriodicalId":89091,"journal":{"name":"Bioengineered bugs","volume":"2 3","pages":"129-35"},"PeriodicalIF":0.0,"publicationDate":"2011-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bbug.2.3.15367","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29910001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

E. coli filament formation induced by heterologous S-layer expression. 异源s层表达诱导大肠杆菌纤维形成。

Bioengineered bugs Pub Date : 2011-05-01 DOI: 10.4161/bbug.2.3.15418

Franziska L Lederer, Tobias J Günther, Johannes Raff, Katrin Pollmann

{"title":"E. coli filament formation induced by heterologous S-layer expression.","authors":"Franziska L Lederer, Tobias J Günther, Johannes Raff, Katrin Pollmann","doi":"10.4161/bbug.2.3.15418","DOIUrl":"https://doi.org/10.4161/bbug.2.3.15418","url":null,"abstract":"Escherichia coli is a rod-shaped intestinal bacterium which has a size of 1.1-1.5 µm x 2.0-6.0 µm. The fast cell division process and the uncomplicated living conditions have turned E. coli into a widely used host in genetic engineering and into one of the best studied microorganisms of all. We used E. coli BL21(DE3) as host for heterologous expression of S-layer proteins of Lysinibacillus sphaericus JG-A12 in order to enable a fast and high efficient protein production. The S-layer expression induced in E. coli an unusual elongation of the cells, thus producing filaments of > 100 µm in length. In the stationary growth phase, E. coli filaments develop tube-like structures that contain E. coli single cells. Fluorescence microscopic analyses of S-layer expressing E. coli cells that were stained with membrane stain FM (®) 5-95 verify the membrane origin of the tubes. Analyses of DAPI stained GFP-S-layer expressing E. coli support the assumption of a disordered cell division that is induced by the huge amount of recombinant S-layer proteins. However, the underlying mechanism is still not characterized in detail. These results describe the occurrence of a novel stable cell form of E. coli as a result of a disordered cell division process. ","PeriodicalId":89091,"journal":{"name":"Bioengineered bugs","volume":"2 3","pages":"178-81"},"PeriodicalIF":0.0,"publicationDate":"2011-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bbug.2.3.15418","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29910002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Caspase and nm23: apoptosis genes linked to the antibacterial response of the Chinese mitten crab. Caspase和nm23:与中华绒螯蟹抗菌反应相关的凋亡基因。

Bioengineered bugs Pub Date : 2011-05-01 DOI: 10.4161/bbug.2.3.15046

Xing-Kun Jin, Wei-Wei Li, Qun Wang

引用次数: 5

Modulation of RNA splicing as a potential treatment for cancer. 将调节 RNA 剪接作为一种潜在的癌症治疗方法。

Bioengineered bugs Pub Date : 2011-05-01 DOI: 10.4161/bbug.2.3.15165

John A Bauman, Ryszard Kole

{"title":"Modulation of RNA splicing as a potential treatment for cancer.","authors":"John A Bauman, Ryszard Kole","doi":"10.4161/bbug.2.3.15165","DOIUrl":"10.4161/bbug.2.3.15165","url":null,"abstract":"Close to 90% of human genes are transcribed into pre-mRNA that undergoes alternative splicing, producing multiple mRNAs and proteins from single genes. This process is largely responsible for human proteome diversity, and about half of genetic disease-causing mutations affect splicing. Splice-switching oligonucleotides (SSOs) comprise an emerging class of antisense therapeutics that modify gene expression by directing pre-mRNA splice site usage. Bauman et al. investigated an SSO that up-regulated the expression of an anti-cancer splice variant while simultaneously eliminating an over-expressed cancer-causing splice variant. This was accomplished by targeting pre-mRNA of the apoptotic regulator Bcl-x, which is alternatively spliced to express anti- and pro-apoptotic splice variants Bcl-xL and Bcl-xS, respectively. High expression of Bcl-xL is a hallmark of many cancers and is considered a general mechanism used by cancer cells to evade apoptosis. Redirection of Bcl-x pre-mRNA splicing from Bcl-xL to -xS by SSO induced apoptotic and chemosensitizing effects in various cancer cell lines. Importantly, the paper shows that delivery of Bcl-x SSO using a lipid nanoparticle redirected Bcl-x splicing and reduced tumor burden in melanoma lung metastases. This was the first demonstration of SSO efficacy in tumors in vivo. SSOs are not limited to be solely potential anti-cancer drugs. SSOs were first applied to repair aberrant splicing in thalassemia, a genetic disease, they have been used to create novel proteins (e.g., ∆7TNFR1), and they have recently progressed to clinical trials for patients with Duchenne muscular dystrophy. ","PeriodicalId":89091,"journal":{"name":"Bioengineered bugs","volume":"2 3","pages":"125-8"},"PeriodicalIF":0.0,"publicationDate":"2011-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3225653/pdf/bbug0203_0125.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29909998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

E phage gene transfection associated to chemotherapeutic agents increases apoptosis in lung and colon cancer cells. 与化疗药物相关的E噬菌体基因转染增加肺癌和结肠癌细胞的凋亡。

Bioengineered bugs Pub Date : 2011-05-01 DOI: 10.4161/bbug.2.3.15533

Ana R Rama, Jose Prados, Consolacion Melguizo, Pablo J Alvarez, Raúl Ortiz, Roberto Madeddu, Antonia Aranega

{"title":"E phage gene transfection associated to chemotherapeutic agents increases apoptosis in lung and colon cancer cells.","authors":"Ana R Rama, Jose Prados, Consolacion Melguizo, Pablo J Alvarez, Raúl Ortiz, Roberto Madeddu, Antonia Aranega","doi":"10.4161/bbug.2.3.15533","DOIUrl":"https://doi.org/10.4161/bbug.2.3.15533","url":null,"abstract":"The limited ability of conventional therapies to achieve the long-term survival of metastatic lung and colon cancer patients suggests the need for new treatment options. In this respect, genes encoding cytotoxic proteins have been proposed as a new strategy to enhance the activity of drugs, and combined therapies involving such genes and classical antitumoral drugs have been studied intensively. The E gene from phiX174 encodes a membrane protein with a toxic domain that leads to a decrease in tumour cell growth rates. Therefore, in order to improve the anti-tumour effects of currently used chemotherapeutic drugs on cancer cells, we investigated the association of the E suicide gene with these antineoplastic drugs. The E gene has antitumoral effects in both lung and colon cancer cells. In addition, expression of this gene induces ultrastructural changes in lung cancer transfected cells (A-549), although the significance of these changes remains unknown. The effect of combined therapy (gene and cytotoxic therapy) enhances the inhibition of tumour cell proliferation in comparison to single treatments. Indeed, our in vitro results indicate that an experimental therapeutic strategy based on this combination of E gene therapy and cytotoxic drugs may result in a new treatment strategy for patients with advanced lung and colon cancer.","PeriodicalId":89091,"journal":{"name":"Bioengineered bugs","volume":"2 3","pages":"163-7"},"PeriodicalIF":0.0,"publicationDate":"2011-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bbug.2.3.15533","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29999152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Identification and characterization of an essential gene in Listeria monocytogenes using an inducible gene expression system. 利用诱导基因表达系统鉴定单核增生李斯特菌必需基因。

Bioengineered bugs Pub Date : 2011-05-01 DOI: 10.4161/bbug.2.3.15476

Kiera M Considine, Roy D Sleator, Alan L Kelly, Gerald F Fitzgerald, Colin Hill

{"title":"Identification and characterization of an essential gene in Listeria monocytogenes using an inducible gene expression system.","authors":"Kiera M Considine, Roy D Sleator, Alan L Kelly, Gerald F Fitzgerald, Colin Hill","doi":"10.4161/bbug.2.3.15476","DOIUrl":"https://doi.org/10.4161/bbug.2.3.15476","url":null,"abstract":"The Listeria monocytogenes gene lmo1594 is a homolog of the Bacillus subtilis cell division gene ezrA. EzrA is a negative regulator of FtsZ ring formation, which is required for efficient cell division as it regulates the frequency and position of Z-rings in the cell and prevents aberrant polar cell division. Previously identified as a putative high pressure (HP) resistance mechanism; conferring enhanced barotolerance when heterologously expressed against an Escherichia coli background; the aim of the current study was to investigate whether lmo1594 plays a role in listerial barotolerance. When the creation of a deletion mutant proved unsuccessful, the role of lmo1594 was addressed by creating a conditional knockout mutant which demonstrated that the gene is in fact essential for cell survival and growth in L. monocytogenes. In order to investigate the effect of lmo1594 on barotolerance, the gene was over-expressed. The over-expression of lmo1594 increased survival levels in L. monocytogenes treated at 300 MPa, but survival levels similar to those of the wild-type strain were observed when treated at a higher pressure (≥400 MPa). In conclusion, this study reveals for the first time that lmo1594 is absolutely essential for listerial cell growth and survival, and also plays an important role in listerial barotolerance.","PeriodicalId":89091,"journal":{"name":"Bioengineered bugs","volume":"2 3","pages":"150-9"},"PeriodicalIF":0.0,"publicationDate":"2011-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bbug.2.3.15476","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29910496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11