Bioengineered bugs最新文献_第4页

Attenuated Bordetella pertussis BPZE1 as a live vehicle for heterologous vaccine antigens delivery through the nasal route. 减毒百日咳杆菌BPZE1作为异源疫苗抗原通过鼻腔传递的活载体。

Bioengineered bugs Pub Date : 2011-11-01 DOI: 10.4161/bbug.2.6.18167

Rui Li, Annabelle Lim, Sylvie Alonso

{"title":"Attenuated Bordetella pertussis BPZE1 as a live vehicle for heterologous vaccine antigens delivery through the nasal route.","authors":"Rui Li, Annabelle Lim, Sylvie Alonso","doi":"10.4161/bbug.2.6.18167","DOIUrl":"https://doi.org/10.4161/bbug.2.6.18167","url":null,"abstract":"Whereas the great majority of the current vaccines are delivered through the parenteral route, mucosal administration has been increasingly considered for controlling infection and preventing disease. Mucosal vaccination can trigger both humoral and cell-mediated protection, not only at the targeted mucosal surface, but also systemically. In this regard, nasal vaccination has shown great potential. The live attenuated strain of Bordetella pertussis, BPZE1, is particularly attractive and promising as a nasal vaccine delivery vector of heterologous antigen vaccine candidates. BPZE1 was originally developed as a live nasal pertussis vaccine candidate, and is currently undergoing phase I clinical trial in human (http://www.child-innovac.org). Highly adapted to the human respiratory tract and offering several potential protein carriers for presentation of the heterologous antigen vaccine candidates, BPZE1 represents an appealing platform for the development of live recombinant vaccines delivered via the nasal route that would confer simultaneous protection against pertussis and the targeted infectious disease(s).","PeriodicalId":89091,"journal":{"name":"Bioengineered bugs","volume":" ","pages":"315-9"},"PeriodicalIF":0.0,"publicationDate":"2011-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bbug.2.6.18167","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40140520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Metabolic engineering to improve 5-aminolevulinic acid production. 代谢工程提高5-氨基乙酰丙酸产量。

Bioengineered bugs Pub Date : 2011-11-01 DOI: 10.4161/bbug.2.6.17237

Zhen Kang, Yang Wang, Qiang Wang, Qingsheng Qi

引用次数: 23

Erratum: "The bacterial ghost platform system—Production and applications" 勘误:“细菌幽灵平台系统-生产与应用”

Bioengineered bugs Pub Date : 2011-11-01 DOI: 10.4161/bbug.2.6.18711

引用次数: 0

Lysins to kill - a tale of viral weapons of mass destruction. 溶酶杀死——一个关于病毒大规模杀伤性武器的故事。

Bioengineered bugs Pub Date : 2011-11-01 DOI: 10.4161/bbug.2.6.16804

Jim O'Mahony, Mark Fenton, Marine Henry, Roy D Sleator, Aidan Coffey

引用次数: 13

Designing symbiosis. 设计共生。

Bioengineered bugs Pub Date : 2011-11-01 DOI: 10.4161/bbug.2.6.16801

Kazufumi Hosoda, Tetsuya Yomo

引用次数: 10

Metabolic regulation of an fnr gene knockout Escherichia coli under oxygen limitation. 缺氧条件下fnr基因敲除大肠杆菌的代谢调控。

Bioengineered bugs Pub Date : 2011-11-01 DOI: 10.4161/bbug.2.6.16350

Lolo Wal Marzan, Khandaker Al Zaid Siddiquee, Kazuyuki Shimizu

{"title":"Metabolic regulation of an fnr gene knockout Escherichia coli under oxygen limitation.","authors":"Lolo Wal Marzan, Khandaker Al Zaid Siddiquee, Kazuyuki Shimizu","doi":"10.4161/bbug.2.6.16350","DOIUrl":"https://doi.org/10.4161/bbug.2.6.16350","url":null,"abstract":"In addition to our previous study on the effect of fnr gene knockout on the metabolism in Escherichia coli under aerobic conditions (Kumar and Shimizu, Microb Cell Fact 2011), here we further investigated the effect of fnr gene knockout on the metabolism under micro-aerobic condition based on gene expressions, enzyme activities and intracellular metabolic fluxes. The objective of the present research is to clarify the metabolic regulation mechanism on how the culture environment, such as oxygen level, affects the cell metabolism in relation to gene expressions, enzyme activities and fluxes via global regulators such as Fnr and ArcA/B systems. Under micro-aerobic condition, the flux through Pfl and Frd were reduced for the mutant, which are due to fnr gene knockout. The decreased flux through Pfl may have caused accumulation of PYR, which increased the flux through LDH. The fnr gene knockout caused arcA to be downregulated, and thus the TCA cycle was activated, and cyoA and cydB genes were upregulated. The downregulation of arcA caused lpdA and aceE, F to be upregulated where the flux through PDHc increased. The fnr gene knockout indirectly caused cra gene transcript level to be decreased, which in turn caused the glycolysis genes to be upregulated, which correspond to the increase in the specific glucose consumption rate. The fnr gene knockout also caused crp transcript level to be increased, where there might be some relationship between the two due to similar structure and gene sequence. It may be quite important to understand the metabolic regulation mechanism based on different levels of information for the efficient metabolic engineering and control of the culture environment for process optimization.","PeriodicalId":89091,"journal":{"name":"Bioengineered bugs","volume":"2 6","pages":"331-7"},"PeriodicalIF":0.0,"publicationDate":"2011-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bbug.2.6.16350","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30217323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

The genetic toolbox for Leishmania parasites. 利什曼原虫的基因工具箱。

Bioengineered bugs Pub Date : 2011-11-01 DOI: 10.4161/bbug.2.6.18205

Sigrid C Roberts

{"title":"The genetic toolbox for Leishmania parasites.","authors":"Sigrid C Roberts","doi":"10.4161/bbug.2.6.18205","DOIUrl":"https://doi.org/10.4161/bbug.2.6.18205","url":null,"abstract":"Leishmania parasites cause a variety of devastating diseases in tropical areas around the world. Due to the lack of vaccines and limited availability of drugs, new therapeutic targets are urgently needed. A variety of genetic tools have been developed to investigate the complex biology of this parasite and its interactions with the host. One of the main techniques is the generation of knock-out parasites via targeted gene replacement, a process that takes advantage of the parasites ability to undergo homologous recombination. Studying the effect of gene deletions in vitro and in infectivity models in vivo allows understanding the function of a target gene and its potential as a therapeutic target. Other genetic manipulations available include episomal and chromosomal complementation and the generation of overproducer strains. However, there are also limitations, such as the lack of RNA interference machinery in most Leishmania species and limited options for inducible expression systems. The genomes of several Leishmania species have now been sequenced and will provide powerful resources in combination with the genetic tools that are available. The increasing knowledge of parasite biology and host parasite interactions derived from these studies will raise the number of potential therapeutic targets, which are sorely needed to combat leishmaniasis.","PeriodicalId":89091,"journal":{"name":"Bioengineered bugs","volume":" ","pages":"320-6"},"PeriodicalIF":0.0,"publicationDate":"2011-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bbug.2.6.18205","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40140612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Shifting from a gene-centric to metabolite-centric strategy to determine the core gut microbiome. 从以基因为中心转向以代谢物为中心的策略来确定核心肠道微生物组。

Bioengineered bugs Pub Date : 2011-11-01 DOI: 10.4161/bbug.2.6.17235

Julian R Marchesi

引用次数: 5

Current knowledge on isobutanol production with Escherichia coli, Bacillus subtilis and Corynebacterium glutamicum. 用大肠杆菌、枯草芽孢杆菌和谷氨酸棒状杆菌生产异丁醇的最新知识。

Bioengineered bugs Pub Date : 2011-11-01 DOI: 10.4161/bbug.2.6.17845

Bastian Blombach, Bernhard J Eikmanns

{"title":"Current knowledge on isobutanol production with Escherichia coli, Bacillus subtilis and Corynebacterium glutamicum.","authors":"Bastian Blombach, Bernhard J Eikmanns","doi":"10.4161/bbug.2.6.17845","DOIUrl":"https://doi.org/10.4161/bbug.2.6.17845","url":null,"abstract":"Due to steadily rising crude oil prices great efforts have been made to develop designer bugs for the fermentative production of higher alcohols, such as 2-methyl-1-butanol, 3-methyl-1-butanol and 2-Methyl-1-propanol (isobutanol), which all possess quality characteristics comparable to traditional oil based fuels. The common metabolic engineering approach uses the last two steps of the Ehrlich pathway, catalyzed by 2-ketoacid decarboxylase and an alcohol dehydrogenase converting the branched chain 2-ketoacids of L-isoleucine, L-leucine, and L-valine into the respective alcohols. This strategy was successfully used to engineer well suited and industrially employed bacteria, such as Escherichia coli, Bacillus subtilis and Corynebacterium glutamicum for the production of higher alcohols. Among these alcohols, isobutanol is currently the most promising one regarding final titer and yield. This article summarizes the current knowledge and achievements on isobutanol production with E. coli, B. subtilis and C. glutamicum regarding the metabolic engineering approaches and process conditions.","PeriodicalId":89091,"journal":{"name":"Bioengineered bugs","volume":"2 6","pages":"346-50"},"PeriodicalIF":0.0,"publicationDate":"2011-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bbug.2.6.17845","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30217317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 86

Online homology modelling as a means of bridging the sequence-structure gap. 在线同源建模作为一种弥合序列结构差距的手段。

Bioengineered bugs Pub Date : 2011-11-01 DOI: 10.4161/bbug.2.6.16116

David Sheehan, Siobhán O'Sullivan

{"title":"Online homology modelling as a means of bridging the sequence-structure gap.","authors":"David Sheehan, Siobhán O'Sullivan","doi":"10.4161/bbug.2.6.16116","DOIUrl":"https://doi.org/10.4161/bbug.2.6.16116","url":null,"abstract":"For even the best-studied species, there is a large gap in their representation in the protein databank (PDB) compared to within sequence databases. Typically, less than 2% of sequences are represented in the PDB. This is partly due to the considerable experimental challenge and manual inputs required to solve three dimensional structures by methods such as X-ray diffraction and multi-dimensional nuclear magnetic resonance (NMR) spectroscopy in comparison to high-throughput sequencing. This gap is made even wider by the high level of redundancy within the PDB and under-representation of some protein categories such as membrane-associated proteins which comprise approximately 25% of proteins encoded in genomes. A traditional route to closing the sequence-structure gap is offered by homology modelling whereby the sequence of a target protein is modelled on a template represented in the PDB using in silico energy minimisation approaches. More recently, online homology servers have become available which automatically generate models from proffered sequences. However, many online servers give little indication of the structural plausibility of the generated model. In this paper, the online homology server Geno3D will be described. This server uses similar software to that used in modelling structures during structure determination and thus generates data allowing determination of the structural plausibility of models. For illustration, modelling of a chemotaxis protein (CheY) from Pseudomononas entomophila L48 (accession YP_609298) on a template (PDB id. 1mvo), the phosphorylation domain of an outer membrane protein PhoP from Bacillus subtilis, will be described.","PeriodicalId":89091,"journal":{"name":"Bioengineered bugs","volume":" ","pages":"299-305"},"PeriodicalIF":0.0,"publicationDate":"2011-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bbug.2.6.16116","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40136200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13