Biomatter最新文献_第6页

Kinetics and isotherm of fibronectin adsorption to three-dimensional porous chitosan scaffolds explored by ¹²⁵I-radiolabelling. 通过¹²5 - 1放射性标记研究了三维多孔壳聚糖支架吸附纤维连接蛋白的动力学和等温线。

Biomatter Pub Date : 2013-04-01 Epub Date: 2013-04-29 DOI: 10.4161/biom.24791

Isabel F Amaral, Susana R Sousa, Ismael Neiva, Lara Marcos-Silva, Charles J Kirkpatrick, Mário A Barbosa, Ana P Pêgo

{"title":"Kinetics and isotherm of fibronectin adsorption to three-dimensional porous chitosan scaffolds explored by ¹²⁵I-radiolabelling.","authors":"Isabel F Amaral, Susana R Sousa, Ismael Neiva, Lara Marcos-Silva, Charles J Kirkpatrick, Mário A Barbosa, Ana P Pêgo","doi":"10.4161/biom.24791","DOIUrl":"https://doi.org/10.4161/biom.24791","url":null,"abstract":"In this study, (125)I-radiolabelling was explored to follow the kinetics and isotherm of fibronectin (FN) adsorption to porous polymeric scaffolds, as well as to assess the elution and exchangeability of pre-adsorbed FN following incubation in serum-containing culture medium. Chitosan (CH) porous scaffolds with two different degrees of acetylation (DA 4% and 15%) were incubated in FN solutions with concentrations ranging from 5 to 50 µg/mL. The kinetic and isotherm of FN adsorption to CH were successfully followed using (125)I-FN as a tracer molecule. While on DA 4% the levels of adsorbed FN increased linearly with FN solution concentration, on DA 15% a saturation plateau was attained, and FN adsorbed amounts were significantly lower. These findings were supported by immunofluorescent studies that revealed, for the same FN solution concentration, higher levels of exposed cell-binding domains on DA 4% as compared with DA 15%. Following incubation in serum containing medium, DA 4% also revealed higher ability to exchange pre-adsorbed FN by new FN molecules from serum than DA 15%. In accordance, when assessing the efficacy of passively adsorbed FN to promote endothelial cell (EC) adhesion to CH, ECs were found to adhere at higher levels to DA 4% as compared with DA 15%, 5 µg/mL of FN being already efficient in promoting cell adhesion and cytoskeletal organization on CH with DA 4%. Taken together the results show that protein radiolabelling can be used as an effective tool to study protein adsorption to porous polymeric scaffolds, both from single and complex protein solutions. ","PeriodicalId":8891,"journal":{"name":"Biomatter","volume":"3 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2013-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/biom.24791","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31491622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Role of culture conditions on in vitro transformation and cellular colonization of biomimetic HA-Col scaffolds. 培养条件对仿生HA-Col支架体外转化和细胞定植的影响。

Biomatter Pub Date : 2013-04-01 DOI: 10.4161/biom.24922

Doris M Campos, Gloria A Soares, Karine Anselme

{"title":"Role of culture conditions on in vitro transformation and cellular colonization of biomimetic HA-Col scaffolds.","authors":"Doris M Campos, Gloria A Soares, Karine Anselme","doi":"10.4161/biom.24922","DOIUrl":"https://doi.org/10.4161/biom.24922","url":null,"abstract":"We have recently developed new 3D hydroxyapatite/collagen (50/50 wt%) scaffolds using a biomimetic synthesis approach. The first in vitro tests performed in static culture showed a limited cell colonization and survival inside the scaffolds. The current study evaluated in dynamic culture the scaffold changes and colonization by human immortalized osteoprogenitor STRO-1A cells. The stability of our scaffolds in the different culture conditions (static, low flow, high flow) was validated by the maintenance of the pore diameter and interconnectivity over 21 d. The colonization and the viability of STRO-1A cells inside the scaffolds were further evaluated on histological sections. It was demonstrated that only the high flow-rate allowed cell survival after 7 d and a complete scaffold colonization. Moreover, the colonization and viability were different in function of the scaffold position inside the perfusion container. The differentiation markers (alkaline phosphatase activity, type I procollagen and osteocalcin synthesis) of STRO-1A cells were analyzed in the culture medium after 7, 14 and 21 d. The low flow-rate increased significantly the three markers compared with static conditions. In contrast, markers were reduced in high flow-rate compared with low flow-rate. To explain this surprising result, we hypothesized that the different molecules were actually adsorbed on the scaffold because of the closed circuit used in the high flow-rate conditions. In summary, this study provides original results on the influence of flow rate but mostly of the circuit used (open/closed) on the structural modifications and cell colonization of collagen-HA scaffolds. ","PeriodicalId":8891,"journal":{"name":"Biomatter","volume":"3 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2013-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/biom.24922","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31466053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

A new synthesis route to high surface area sol gel bioactive glass through alcohol washing: a preliminary study. 酒精洗涤法合成高表面积溶胶-凝胶生物活性玻璃新途径的初步研究。

Biomatter Pub Date : 2013-04-01 Epub Date: 2013-03-19 DOI: 10.4161/biom.24288

Lakshmi M Mukundan, Remya Nirmal, Dhanesh Vaikkath, Prabha D Nair

{"title":"A new synthesis route to high surface area sol gel bioactive glass through alcohol washing: a preliminary study.","authors":"Lakshmi M Mukundan, Remya Nirmal, Dhanesh Vaikkath, Prabha D Nair","doi":"10.4161/biom.24288","DOIUrl":"https://doi.org/10.4161/biom.24288","url":null,"abstract":"Bioactive glass is one of the widely used bone repair material due to its unique properties like osteoconductivity, osteoinductivity and biodegradability. In this study bioactive glass is prepared by the sol gel process and stabilized by a novel method that involves a solvent instead of the conventional calcinations process. This study represents the first attempt to use this method for the stabilization of bioactive glass. The bioactive glass stabilized by this ethanol washing process was characterized for its physicochemical and biomimetic property in comparison with similar composition of calcined bioactive glass. The compositional similarity of the two stabilized glass powders was confirmed by spectroscopic and thermogravimetric analysis. Other physicochemical characterizations together with the cell culture studies with L929 fibroblast cells and bone marrow mesenchymal stem cells proved that the stabilization was achieved with the retention of its inherent bioactive potential. However an increase in the surface area of the glass powder was obtained as a result of this ethanol washing process and this add up to the success of the study. Hence the present study exhibits a promising route for high surface area bioactive glass for increasing biomimicity. ","PeriodicalId":8891,"journal":{"name":"Biomatter","volume":"3 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2013-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/biom.24288","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31414056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34

Synthesis of spherical calcium phosphate particles for dental and orthopedic applications. 合成用于牙科和整形外科的球形磷酸钙颗粒。

Biomatter Pub Date : 2013-04-01 DOI: 10.4161/biom.25103

Marc Bohner, Solène Tadier, Noémie van Garderen, Alex de Gasparo, Nicola Döbelin, Gamal Baroud

{"title":"Synthesis of spherical calcium phosphate particles for dental and orthopedic applications.","authors":"Marc Bohner, Solène Tadier, Noémie van Garderen, Alex de Gasparo, Nicola Döbelin, Gamal Baroud","doi":"10.4161/biom.25103","DOIUrl":"10.4161/biom.25103","url":null,"abstract":"Calcium phosphate materials have been used increasingly in the past 40 years as bone graft substitutes in the dental and orthopedic fields. Accordingly, numerous fabrication methods have been proposed and used. However, the controlled production of spherical calcium phosphate particles remains a challenge. Since such particles are essential for the synthesis of pastes and cements delivered into the host bone by minimally-invasive approaches, the aim of the present document is to review their synthesis and applications. For that purpose, production methods were classified according to the used reagents (solutions, slurries, pastes, powders), dispersion media (gas, liquid, solid), dispersion tools (nozzle, propeller, sieve, mold), particle diameters of the end product (from 10 nm to 10 mm), and calcium phosphate phases. Low-temperature calcium phosphates such as monetite, brushite or octacalcium phosphate, as well as high-temperature calcium phosphates, such as hydroxyapatite, β-tricalcium phosphate or tetracalcium phosphate, were considered. More than a dozen production methods and over hundred scientific publications were discussed. ","PeriodicalId":8891,"journal":{"name":"Biomatter","volume":"3 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2013-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/03/77/biom-3-e25103.PMC3749799.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31466682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inclusion of Biomatter in Medline (Index Medicus) 生物物质在Medline (Index Medicus)中的收录

Biomatter Pub Date : 2013-04-01 DOI: 10.4161/BIOM.24610

P. Granja

{"title":"Inclusion of Biomatter in Medline (Index Medicus)","authors":"P. Granja","doi":"10.4161/BIOM.24610","DOIUrl":"https://doi.org/10.4161/BIOM.24610","url":null,"abstract":"It is with great pleasure that we announce the selection of Biomatter for indexing in Medline (Index Medicus). \u0000 \u0000Medline, which is Part of the US National Library of Medicine, is freely available through PubMed and is the most prestigious and widely used database of biomedical literature. Considering Medline’s stringent requirements for indexing new journals, this inclusion confirms the quality of Biomatter. Being indexed will be instrumental in increasing the visibility of the journal and thus reaching more readers, who find the articles they are interested in through Pubmed. Medline has back-indexed Biomatter’s contents since its first issue in September 2011. \u0000 \u0000The achievement of this important milestone for the journal would not have been possible without the help and support of our Editorial Board members, our reviewers, our authors and our Editorial team. I especially thank our current Editors, Liz Gilmer, Betsy Granger and Kimberly Mitchell, as well as our past Editors Kathryn Sauceda, Tara Barton and Kristine Pipit, for their thorough and timely efforts in several aspects involved in bringing this journal’s contents to its audience. \u0000 \u0000This inclusion in Medline also increases our responsibility and commitment to assure the quality of Biomatter’s scientific content, given the expectable increase in submissions, which will require even more rigor in the selection of accepted manuscripts.","PeriodicalId":8891,"journal":{"name":"Biomatter","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2013-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73958043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An in vitro study of composites of poly(L-lactide-co-ε-caprolactone), β-tricalcium phosphate and ciprofloxacin intended for local treatment of osteomyelitis. 聚l -乳酸-co-ε-己内酯、β-磷酸三钙和环丙沙星复合材料局部治疗骨髓炎的体外研究。

Biomatter Pub Date : 2013-04-01 Epub Date: 2013-01-01 DOI: 10.4161/biom.23162

Niina Ahola, Noora Männistö, Minna Veiranto, Matti Karp, Jaana Rich, Alexander Efimov, Jukka Seppälä, Minna Kellomäki

{"title":"An in vitro study of composites of poly(L-lactide-co-ε-caprolactone), β-tricalcium phosphate and ciprofloxacin intended for local treatment of osteomyelitis.","authors":"Niina Ahola, Noora Männistö, Minna Veiranto, Matti Karp, Jaana Rich, Alexander Efimov, Jukka Seppälä, Minna Kellomäki","doi":"10.4161/biom.23162","DOIUrl":"https://doi.org/10.4161/biom.23162","url":null,"abstract":"Osteomyelitis is a bacterial disease that can become chronic, and treatment often includes a surgical operation to remove infected bone. The aim of this study was to develop and investigate in vitro bone filling composite materials that release ciprofloxacin to kill any remaining bacteria and contain bioceramic to help the bone to heal. Three composites of poly(L-lactide-co-ε-caprolactone), β-tricalcium phosphate and ciprofloxacin were compounded using twin-screw extrusion and sterilized by gamma irradiation. Drug release and degradation of the composites were investigated in vitro for 52 weeks. The composite with 50 wt% of β-TCP had the most promising ciprofloxacin release profile. The ceramic component accelerated the drug release that occurred in three phases obeying first-order kinetics. Inhibition zone testing using bioluminescence showed that the released ciprofloxacin had effect in eradicating a common osteomyelitis causing bacteria Pseudomonas aeruginosa. During the in vitro degradation test series, molar weight of the polymer matrix of the composites decreased rapidly. Additionally, (1)H-NMR analysis showed that the polymer had blocky structure and the comonomer ratio changed during hydrolysis. The tested composites showed great potential to be developed into bone filler materials for the treatment of osteomyelitis or other bone related infections. ","PeriodicalId":8891,"journal":{"name":"Biomatter","volume":"3 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2013-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/biom.23162","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31317910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

Formulation and optimization of temozolomide nanoparticles by 3 factor 2 level factorial design. 替莫唑胺纳米颗粒的3因子2水平析因设计及优化。

Biomatter Pub Date : 2013-04-01 DOI: 10.4161/biom.25102

Aviral Jain, Sanjay K Jain

{"title":"Formulation and optimization of temozolomide nanoparticles by 3 factor 2 level factorial design.","authors":"Aviral Jain, Sanjay K Jain","doi":"10.4161/biom.25102","DOIUrl":"https://doi.org/10.4161/biom.25102","url":null,"abstract":"The aim of this study was to investigate the combined influence of 3 independent variables in the preparation of temozolomide bearing Non-PEGylated and PEGylated nanoparticles by emulsification solvent evaporation method. A 3 factor 2 level design was used to derive a polynomial quadratic model and construct contour plots to predict responses. The independent variables selected were concentration of drug (A), concentration of PLGA/PEG-PLGA (B), PVA concentration in aqueous phase (C) and sonication time (D) and evaluated for percentage drug entrapment (PDE) and particle size (PS). A 3(4) factorial design was used with 4 factors (A, B, C and D) at 3 levels and experimental trials were performed at all 82 possible combinations. In the present work, 28 runs are considered as the preliminary trials revealed that on increasing drug concentration from 2.5 to 5 mg the percent drug entrapment increases, but on further increasing the drug concentration (i.e., to 7.5 mg) no significant effect on the percent drug entrapment and particle size was observed. The 3(4) factorial design was used to derive a polynomial quadratic model and construct contour plots to predict responses. Contour plots were constructed to show the effects of A, B, C and D on the PDE and PS.","PeriodicalId":8891,"journal":{"name":"Biomatter","volume":"3 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2013-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/biom.25102","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31466680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

Defining cell-matrix combination products in the era of pluripotency. 在多能时代定义细胞-基质组合产品。

Biomatter Pub Date : 2013-01-01 DOI: 10.4161/biom.24496

Hal Sternberg, Jeffrey Janus, Michael D West

{"title":"Defining cell-matrix combination products in the era of pluripotency.","authors":"Hal Sternberg, Jeffrey Janus, Michael D West","doi":"10.4161/biom.24496","DOIUrl":"https://doi.org/10.4161/biom.24496","url":null,"abstract":"Human pluripotent stem (hPS) cells provide an attractive opportunity for the manufacture of a wide array of therapeutic cell types. The challenges to commercialization include the thousand-fold diversity of cell types emerging from hPS cells and the associated difficulties in validating processes to reliably generate cells with precise identity and purity. Improved methods of controlling the dosage and migration of hPS-derived cells in solid tissues are also needed. To directly address these issues, we clonally expanded proliferating lineages of cells that were intermediate in regard to their state of differentiation between hPS and terminally differentiated cells. These cells called monoclonal embryonic progenitors (hEP), are expandable mortal lineages with diverse site-specific homeobox gene expression and multipotentiality. In this review, we discuss methods of generating combination products wherein the fate space of precisely identified monoclonal hEP cells is mapped by differentiating the cells in vitro in HyStem-3D bead arrays in the presence of diverse growth factors. This combination of discovery processes has the potential to translate directly into cell-matrix formulations that can be used to generate pre-clinical data leading to human clinical trials and potentially new medical therapies. ","PeriodicalId":8891,"journal":{"name":"Biomatter","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/biom.24496","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31341243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Encapsulated stem cells for cancer therapy. 包封干细胞用于癌症治疗。

Biomatter Pub Date : 2013-01-01 DOI: 10.4161/biom.24278

Khalid Shah

引用次数: 42

Enhancing retention and efficacy of cardiosphere-derived cells administered after myocardial infarction using a hyaluronan-gelatin hydrogel. 使用透明质酸明胶水凝胶增强心肌梗死后心球源性细胞的保留和功效。

Biomatter Pub Date : 2013-01-01 DOI: 10.4161/biom.24490

Rachel Ruckdeschel Smith, Eduardo Marbán, Linda Marbán

引用次数: 47