Biomatter最新文献_第2页

Dextran grafting on PTFE surface for cardiovascular applications. 聚四氟乙烯表面葡聚糖接枝心血管应用。

Biomatter Pub Date : 2014-01-01 DOI: 10.4161/biom.28805

Eléonore C Michel, Vanessa Montaño-Machado, Pascale Chevallier, Amélie Labbé-Barrère, Didier Letourneur, Diego Mantovani

{"title":"Dextran grafting on PTFE surface for cardiovascular applications.","authors":"Eléonore C Michel, Vanessa Montaño-Machado, Pascale Chevallier, Amélie Labbé-Barrère, Didier Letourneur, Diego Mantovani","doi":"10.4161/biom.28805","DOIUrl":"https://doi.org/10.4161/biom.28805","url":null,"abstract":"The modification of biomaterial surfaces with biomolecules influences the biological response. In this work, caboxymethyldextrans (CMD) with different degrees of substitution have been grafted to surfaces by introduction of amino moieties directly onto the substrate surface. Polytetrafluoroethylene was selected as a model substrate for biomaterial as it is already largely used for cardiovascular clinical applications. Firstly, CMD polymers were characterized by FTIR, (1)H-NMR, and conductimetric titration. Then, the coatings have been analyzed by XPS to confirm the grafting and determine the composition. Once characterized, biological performances of CMD coatings were investigated. The hemocompatibility was ascertained using the free hemoglobin method. The effects on endothelial and smooth muscle cell adhesion were also studied. Results indicated that CMD at a 0.2 substitution degree, significantly influenced the biological property of PTFE by exhibiting non-thrombogenic properties as well as enhancing endothelial cell adhesion along with limiting smooth muscle cell adhesion. This work suggested the creation of versatile pro-active biomaterials suitable for different biomedical applications. ","PeriodicalId":8891,"journal":{"name":"Biomatter","volume":"4 ","pages":"e28805"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/biom.28805","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32888183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Interfacing polymeric scaffolds with primary pancreatic ductal adenocarcinoma cells to develop 3D cancer models. 将聚合物支架与原发性胰腺导管腺癌细胞结合，建立三维肿瘤模型。

Biomatter Pub Date : 2014-01-01 DOI: 10.4161/21592527.2014.955386

Claudio Ricci, Carlos Mota, Stefania Moscato, Delfo D'Alessandro, Stefano Ugel, Silvia Sartoris, Vincenzo Bronte, Ugo Boggi, Daniela Campani, Niccola Funel, Lorenzo Moroni, Serena Danti

{"title":"Interfacing polymeric scaffolds with primary pancreatic ductal adenocarcinoma cells to develop 3D cancer models.","authors":"Claudio Ricci, Carlos Mota, Stefania Moscato, Delfo D'Alessandro, Stefano Ugel, Silvia Sartoris, Vincenzo Bronte, Ugo Boggi, Daniela Campani, Niccola Funel, Lorenzo Moroni, Serena Danti","doi":"10.4161/21592527.2014.955386","DOIUrl":"https://doi.org/10.4161/21592527.2014.955386","url":null,"abstract":"We analyzed the interactions between human primary cells from pancreatic ductal adenocarcinoma (PDAC) and polymeric scaffolds to develop 3D cancer models useful for mimicking the biology of this tumor. Three scaffold types based on two biocompatible polymeric formulations, such as poly(vinyl alcohol)/gelatin (PVA/G) mixture and poly(ethylene oxide terephthalate)/poly(butylene terephthalate) (PEOT/PBT) copolymer, were obtained via different techniques, namely, emulsion and freeze-drying, compression molding followed by salt leaching, and electrospinning. In this way, primary PDAC cells interfaced with different pore topographies, such as sponge-like pores of different shape and size or nanofiber interspaces. The aim of this study was to investigate the influence played by the scaffold architecture over cancerous cell growth and function. In all scaffolds, primary PDAC cells showed good viability and synthesized tumor-specific metalloproteinases (MMPs) such as MMP-2, and MMP-9. However, only sponge-like pores, obtained via emulsion-based and salt leaching-based techniques allowed for an organized cellular aggregation very similar to the native PDAC morphological structure. Differently, these cell clusters were not observed on PEOT/PBT electrospun scaffolds. MMP-2 and MMP-9, as active enzymes, resulted to be increased in PVA/G and PEOT/PBT sponges, respectively. These findings suggested that spongy scaffolds supported the generation of pancreatic tumor models with enhanced aggressiveness. In conclusion, primary PDAC cells showed diverse behaviors while interacting with different scaffold types that can be potentially exploited to create stage-specific pancreatic cancer models likely to provide new knowledge on the modulation and drug susceptibility of MMPs. ","PeriodicalId":8891,"journal":{"name":"Biomatter","volume":"4 ","pages":"e955386"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/21592527.2014.955386","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32888171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 46

Magnetic force microscopy: quantitative issues in biomaterials. 磁力显微镜:生物材料的定量问题。

Biomatter Pub Date : 2014-01-01 Epub Date: 2014-07-22 DOI: 10.4161/biom.29507

Daniele Passeri, Chunhua Dong, Melania Reggente, Livia Angeloni, Mario Barteri, Francesca A Scaramuzzo, Francesca De Angelis, Fiorenzo Marinelli, Flavia Antonelli, Federica Rinaldi, Carlotta Marianecci, Maria Carafa, Angela Sorbo, Daniela Sordi, Isabel Wce Arends, Marco Rossi

{"title":"Magnetic force microscopy: quantitative issues in biomaterials.","authors":"Daniele Passeri, Chunhua Dong, Melania Reggente, Livia Angeloni, Mario Barteri, Francesca A Scaramuzzo, Francesca De Angelis, Fiorenzo Marinelli, Flavia Antonelli, Federica Rinaldi, Carlotta Marianecci, Maria Carafa, Angela Sorbo, Daniela Sordi, Isabel Wce Arends, Marco Rossi","doi":"10.4161/biom.29507","DOIUrl":"https://doi.org/10.4161/biom.29507","url":null,"abstract":"Magnetic force microscopy (MFM) is an atomic force microscopy (AFM) based technique in which an AFM tip with a magnetic coating is used to probe local magnetic fields with the typical AFM spatial resolution, thus allowing one to acquire images reflecting the local magnetic properties of the samples at the nanoscale. Being a well established tool for the characterization of magnetic recording media, superconductors and magnetic nanomaterials, MFM is finding constantly increasing application in the study of magnetic properties of materials and systems of biological and biomedical interest. After reviewing these latter applications, three case studies are presented in which MFM is used to characterize: (i) magnetoferritin synthesized using apoferritin as molecular reactor; (ii) magnetic nanoparticles loaded niosomes to be used as nanocarriers for drug delivery; (iii) leukemic cells labeled using folic acid-coated core-shell superparamagnetic nanoparticles in order to exploit the presence of folate receptors on the cell membrane surface. In these examples, MFM data are quantitatively analyzed evidencing the limits of the simple analytical models currently used. Provided that suitable models are used to simulate the MFM response, MFM can be used to evaluate the magnetic momentum of the core of magnetoferritin, the iron entrapment efficiency in single vesicles, or the uptake of magnetic nanoparticles into cells. ","PeriodicalId":8891,"journal":{"name":"Biomatter","volume":"4 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/biom.29507","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32527494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 46

Nanocarbon surfaces for biomedicine. 用于生物医学的纳米碳表面。

Biomatter Pub Date : 2014-01-01 Epub Date: 2014-03-19 DOI: 10.4161/biom.28537

Giacomo Reina, Emanuela Tamburri, Silvia Orlanducci, Stefano Gay, Roberto Matassa, Valeria Guglielmotti, Teresa Lavecchia, Maria Letizia Terranova, Marco Rossi

引用次数: 20

Influence of ECAP process on mechanical and corrosion properties of pure Mg and ZK60 magnesium alloy for biodegradable stent applications. ECAP工艺对纯Mg和ZK60镁合金生物可降解支架机械性能和腐蚀性能的影响

Biomatter Pub Date : 2014-01-01 DOI: 10.4161/biom.28283

Ehsan Mostaed, Maurizio Vedani, Mazdak Hashempour, Massimiliano Bestetti

{"title":"Influence of ECAP process on mechanical and corrosion properties of pure Mg and ZK60 magnesium alloy for biodegradable stent applications.","authors":"Ehsan Mostaed, Maurizio Vedani, Mazdak Hashempour, Massimiliano Bestetti","doi":"10.4161/biom.28283","DOIUrl":"https://doi.org/10.4161/biom.28283","url":null,"abstract":"Equal channel angular pressing (ECAP) was performed on ZK60 alloy and pure Mg in the temperature range 150-250 °C. A significant grain refinement was detected after ECAP, leading to an ultrafine grain size (UFG) and enhanced formability during extrusion process. Comparing to conventional coarse grained samples, fracture elongation of pure Mg and ZK60 alloy were significantly improved by 130% and 100%, respectively, while the tensile strength remained at high level. Extrusion was performed on ECAP processed billets to produce small tubes (with outer/inner diameter of 4/2.5 mm) as precursors for biodegradable stents. Studies on extruded tubes revealed that even after extrusion the microstructure and microhardness of the UFG ZK60 alloy were almost stable. Furthermore, pure Mg tubes showed an additional improvement in terms of grain refining and mechanical properties after extrusion. Electrochemical analyses and microstructural assessments after corrosion tests demonstrated two major influential factors in corrosion behavior of the investigated materials. The presence of Zn and Zr as alloying elements simultaneously increases the nobility by formation of a protective film and increase the local corrosion damage by amplifying the pitting development. ECAP treatment decreases the size of the second phase particles thus improving microstructure homogeneity, thereby decreasing the localized corrosion effects. ","PeriodicalId":8891,"journal":{"name":"Biomatter","volume":"4 ","pages":"e28283"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/biom.28283","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32888180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 83

Properties of titanium-alloyed DLC layers for medical applications. 医用钛合金DLC层的性能。

Biomatter Pub Date : 2014-01-01 Epub Date: 2014-08-05 DOI: 10.4161/biom.29505

Ludek Joska, Jaroslav Fojt, Ladislav Cvrcek, Vitezslav Brezina

{"title":"Properties of titanium-alloyed DLC layers for medical applications.","authors":"Ludek Joska, Jaroslav Fojt, Ladislav Cvrcek, Vitezslav Brezina","doi":"10.4161/biom.29505","DOIUrl":"https://doi.org/10.4161/biom.29505","url":null,"abstract":"DLC-type layers offer a good potential for application in medicine, due to their excellent tribological properties, chemical resistance, and bio-inert character. The presented study has verified the possibility of alloying DLC layers with titanium, with coatings containing three levels of titanium concentration prepared. Titanium was present on the surface mainly in the form of oxides. Its increasing concentration led to increased presence of titanium carbide as well. The behavior of the studied systems was stable during exposure in a physiological saline solution. Electrochemical impedance spectra practically did not change with time. Alloying, however, changed the electrochemical behavior of coated systems in a significant way: from inert surface mediating only exchange reactions of the environment in the case of unalloyed DLC layers to a response corresponding rather to a passive surface in the case of alloyed specimens. The effect of DLC layers alloying with titanium was tested by the interaction with a simulated body fluid, during which precipitation of a compound containing calcium and phosphorus--basic components of the bone apatite--occurred on all doped specimens, in contrast to pure DLC. The results of the specimens' surface colonization with cells test proved the positive effect of titanium in the case of specimens with a medium and highest content of this element.","PeriodicalId":8891,"journal":{"name":"Biomatter","volume":"4 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/biom.29505","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32561209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

The effects of a SiO2 coating on the corrosion parameters cpTi and Ti-6Al-7Nb alloy. 研究了SiO2涂层对cpTi和Ti-6Al-7Nb合金腐蚀参数的影响。

Biomatter Pub Date : 2014-01-01 DOI: 10.4161/biom.28535

Marcin Basiaga, Witold Walke, Zbigniew Paszenda, Paweł Karasiński, Janusz Szewczenko

引用次数: 12

Alternative technique for calcium phosphate coating on titanium alloy implants. 钛合金植入物上磷酸钙涂层的替代技术。

Biomatter Pub Date : 2014-01-01 Epub Date: 2014-03-19 DOI: 10.4161/biom.28534

Van Quang Le, Geneviève Pourroy, Andrea Cochis, Lia Rimondini, Wafa I Abdel-Fattah, Hadeer I Mohammed, Adele Carradò

引用次数: 17

Poly(vinyl alcohol) hydrogel coatings with tunable surface exposure of hydroxyapatite. 羟基磷灰石表面暴露可调的聚乙烯醇水凝胶涂层。

Biomatter Pub Date : 2014-01-01 DOI: 10.4161/biom.28764

David Moreau, Arthur Villain, David N Ku, Laurent Corté

{"title":"Poly(vinyl alcohol) hydrogel coatings with tunable surface exposure of hydroxyapatite.","authors":"David Moreau, Arthur Villain, David N Ku, Laurent Corté","doi":"10.4161/biom.28764","DOIUrl":"https://doi.org/10.4161/biom.28764","url":null,"abstract":"Insufficient bone anchoring is a major limitation of artificial substitutes for connective osteoarticular tissues. The use of coatings containing osseoconductive ceramic particles is one of the actively explored strategies to improve osseointegration and strengthen the bone-implant interface for general tissue engineering. Our hypothesis is that hydroxyapatite (HA) particles can be coated robustly on specific assemblies of PVA hydrogel fibers for the potential anchoring of ligament replacements. A simple dip-coating method is described to produce composite coatings made of microscopic hydroxyapatite (HA) particles dispersed in a poly(vinyl alcohol) (PVA) matrix. The materials are compatible with the requirements for implant Good Manufacturing Practices. They are applied to coat bundles of PVA hydrogel fibers used for the development of ligament implants. By means of optical and electronic microscopy, we show that the coating thickness and surface state can be adjusted by varying the composition of the dipping solution. Quantitative analysis based on backscattered electron microscopy show that the exposure of HA at the coating surface can be tuned from 0 to over 55% by decreasing the weight ratio of PVA over HA from 0.4 to 0.1. Abrasion experiments simulating bone-implant contact illustrate how the coating cohesion and wear resistance increase by increasing the content of PVA relative to HA. Using pullout experiments, we find that these coatings adhere well to the fiber bundles and detach by propagation of a crack inside the coating. These results provide a guide to select coated implants for anchoring artificial ligaments. ","PeriodicalId":8891,"journal":{"name":"Biomatter","volume":"4 ","pages":"e28764"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/biom.28764","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32888182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Design of 2D chitosan scaffolds via electrochemical structuring. 基于电化学结构的二维壳聚糖支架设计。

Biomatter Pub Date : 2014-01-01 Epub Date: 2014-06-18 DOI: 10.4161/biom.29506

Lina Altomare, Elena Guglielmo, Elena Maria Varoni, Serena Bertoldi, Andrea Cochis, Lia Rimondini, Luigi De Nardo

{"title":"Design of 2D chitosan scaffolds via electrochemical structuring.","authors":"Lina Altomare, Elena Guglielmo, Elena Maria Varoni, Serena Bertoldi, Andrea Cochis, Lia Rimondini, Luigi De Nardo","doi":"10.4161/biom.29506","DOIUrl":"https://doi.org/10.4161/biom.29506","url":null,"abstract":"Chitosan (CS) is a versatile biopolymer whose morphological and chemico-physical properties can be designed for a variety of biomedical applications. Taking advantage of its electrolytic nature, cathodic polarization allows CS deposition on electrically conductive substrates, resulting in thin porous structures with tunable morphology. Here we propose an easy method to obtain CS membranes with highly oriented micro-channels for tissue engineering applications, relying on simple control of process parameters and cathodic substrate geometry. Cathodic deposition was performed on two different aluminum grids in galvanostatic conditions at 6.25 mA cm(-2) from CS solution [1g L(-1)] in acetic acid (pH 3.5). Self-standing thin scaffolds were cross linked either with genipin or epichlorohydrin, weighted, and observed by optical and electron microscopy. Swelling properties at pH 5 and pH 7.4 have been also investigated and tensile tests performed on swollen samples at room temperature. Finally, direct and indirect assays have been performed to evaluate the cytotoxicity at 24 and 72 h. Thin scaffolds with two different oriented porosities (1000 µm and 500 µm) have been successfully fabricated by electrochemical techniques. Both cross-linking agents did not affected the mechanical properties and cytocompatibility of the resulting structures. Depending on the pH, these structures show interesting swelling properties that can be exploited for drug delivery systems. Moreover, thanks to the possibility of controlling the porosity and the micro-channel orientation, they should be used for the regeneration of tissues requiring a preferential cells orientation, e.g., cardiac patches or ligament regeneration.","PeriodicalId":8891,"journal":{"name":"Biomatter","volume":"4 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/biom.29506","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32561679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11