Inmunologia (Barcelona, Spain : 1987)最新文献_第5页

Informe de actividades de la Sociedad Española de Inmunología 西班牙免疫学会的活动报告

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2013-01-01 DOI: 10.1016/j.inmuno.2013.01.001

José R. Regueiro

引用次数: 1

¿Hacia dónde va la Sociedad Española de Inmunología? 西班牙免疫学会的发展方向是什么?

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2013-01-01 DOI: 10.1016/j.inmuno.2012.11.003

José R. Regueiro, Eduard Palou, Margarita del Val, Miguel Fernández-Arquero, María Luisa Vargas Pérez, Carlos López-Larrea, Luisa M. Villar, José A. Brieva, Eduardo López-Granados, Manuel Muro Amador, Oscar de la Calle-Martin

引用次数: 1

Antiphospholipid antibodies in Mexican HIV-positive patients 墨西哥hiv阳性患者的抗磷脂抗体

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2013-01-01 DOI: 10.1016/j.inmuno.2012.10.002

Elena Soto-Vega , Alejandro Ruiz-Argüelles , Claudia Mendoza-Pinto , Jonathan R. Hernández-Molina , Jose A. Varela-Cabrera , María J. Muñoz-Pérez , Nancy Labastida-Mercado , Mario García-Carrasco , Liliana Rivadeneyra-Espinoza , Carlos Arroyo

{"title":"Antiphospholipid antibodies in Mexican HIV-positive patients","authors":"Elena Soto-Vega , Alejandro Ruiz-Argüelles , Claudia Mendoza-Pinto , Jonathan R. Hernández-Molina , Jose A. Varela-Cabrera , María J. Muñoz-Pérez , Nancy Labastida-Mercado , Mario García-Carrasco , Liliana Rivadeneyra-Espinoza , Carlos Arroyo","doi":"10.1016/j.inmuno.2012.10.002","DOIUrl":"10.1016/j.inmuno.2012.10.002","url":null,"abstract":"<div><p>Several studies have shown that HIV patients tend to develop autoimmune diseases, and have numerous antibodies, such as antiphospholipid antibodies<span>. Antiphospholipid antibodies are the serological markers used in the diagnosis of the antiphospholipid syndrome. However, antiphospholipid antibodies also appear to exist in infectious diseases.</span></p></div><div><h3>Objective</h3><p>To measure the titers of antiphospholipid antibodies in healthy and in HIV positive Mexican mestizo patients, and correlate them with the patient clinical manifestations to identify possible findings compatible with an autoimmune disease.</p></div><div><h3>Material and methods</h3><p>A case control study was conducted on 50 healthy mixed race Mexican subjects and in 50 randomly selected HIV-positive patients from the Infectious Diseases Department of a Regional Hospital in Puebla, México. Antiphospholipid titers were performed on the patients and controls and analyzed to see if there was any correlation between clinical signs.</p></div><div><h3>Results</h3><p><span>There was a statistical difference in the titers of anticardiolipin antibodies </span>isotype<span> IgG between the control group and the HIV group. When sexual preference was evaluated in the HIV group a statistical difference in the antibody titers was observed between homosexual and heterosexual HIV patients.</span></p></div><div><h3>Conclusion</h3><p>There were no correlations found between the antibody titers and specific clinical manifestations in HIV positive patients. The exact clinical meaning of the presence of these antibodies in HIV positive patients is still unknown, so further studies are needed.</p></div>","PeriodicalId":88896,"journal":{"name":"Inmunologia (Barcelona, Spain : 1987)","volume":"32 1","pages":"Pages 12-16"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.inmuno.2012.10.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54642652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

El descenso del número de linfocitos B de memoria en pacientes con inmunodeficiencia variable común está asociado a una apoptosis aumentada de esta población celular. Desarrollo de un método nuevo para la detección de la apoptosis en muestras de sangre completa 在常见可变免疫缺陷患者中，记忆B细胞数量的减少与该细胞群的凋亡增加有关。一种检测全血样本细胞凋亡的新方法的发展

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2013-01-01 DOI: 10.1016/j.inmuno.2013.01.002

Fernanda Gabriela Silva-Carreras, Santiago Sobrecasas, Inmaculada Toboso de Lamo, José Luis Castañer Alabau, Ernesto Roldán Santiago

{"title":"El descenso del número de linfocitos B de memoria en pacientes con inmunodeficiencia variable común está asociado a una apoptosis aumentada de esta población celular. Desarrollo de un método nuevo para la detección de la apoptosis en muestras de sangre completa","authors":"Fernanda Gabriela Silva-Carreras, Santiago Sobrecasas, Inmaculada Toboso de Lamo, José Luis Castañer Alabau, Ernesto Roldán Santiago","doi":"10.1016/j.inmuno.2013.01.002","DOIUrl":"https://doi.org/10.1016/j.inmuno.2013.01.002","url":null,"abstract":"<div><p>Common Variable Immunodeficiency (CVID) is a heterogeneous disease characterised by low memory B cell counts in peripheral blood (PB). Although previous reports have attributed memory CD27+ B cell decrease to a possible defective germinal centre development, the cause of this defect remains basically unknown. On the other hand, increased apoptosis has been implicated in the pathogenesis of several diseases, and could be another factor that contributes to explain memory B cell reduction. However, a negative balance between apoptosis/survival of memory B cells in CVID patients has still not been documented. Therefore, we asked whether increased apoptosis was relevant in CVID. To test this concept, total and memory B cells from CVID patients and healthy controls were studied by flow cytometry to determine spontaneous and induced apoptosis. Apoptosis was measured by Annexin-V expression after a 24 hour culture with and without anti-CD95 proapoptotic monoclonal antibody. We also developed a new and sensitive method that uses 7-amino-actinomycin D (7-AAD) to measure very low numbers of apoptotic cells in whole PB. Our results clearly indicate diminished absolute counts and higher apoptosis in memory B cells from CVID patients than in healthy controls (<em>P</em>  <!-->.01). Moreover, we also demonstrate that CD95 is not implicated in regulating memory B cell apoptosis.</p></div>","PeriodicalId":88896,"journal":{"name":"Inmunologia (Barcelona, Spain : 1987)","volume":"32 1","pages":"Pages 17-24"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.inmuno.2013.01.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92283952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging roles of granulocytes in B cell responses 粒细胞在B细胞反应中的新作用

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2013-01-01 DOI: 10.1016/j.inmuno.2012.08.003

Irene Puga , Montserrat Cols , Andrea Cerutti

{"title":"Emerging roles of granulocytes in B cell responses","authors":"Irene Puga , Montserrat Cols , Andrea Cerutti","doi":"10.1016/j.inmuno.2012.08.003","DOIUrl":"10.1016/j.inmuno.2012.08.003","url":null,"abstract":"<div><p><span>Protective antibody responses require cognate interaction between B cells and </span>T helper cells<span><span><span> in the germinal center of </span>lymphoid follicles<span>. This interaction leads to the formation of plasma cells that secrete high-affinity antibodies of different classes with distinct effector functions. Growing evidence shows that B cells receive additional helper signals from a variety of cells of the innate immune system<span>, including dendritic cells, macrophages, follicular dendritic cells and epithelial cells. </span></span></span>Granulocytes are a fundamental component of the innate immune system, as they are the first leukocytes that infiltrate infection and inflammation sites in order to clear invading microbes and necrotic cells. Granulocytes utilize opsonizing antibodies to enhance their phagocytic and killer functions, but recent studies indicate that granulocytes also optimize antibody diversification and production. In this article, the mechanisms by which different subsets of granulocytes deliver helper signals to B cells and plasma cells are discussed.</span></p></div>","PeriodicalId":88896,"journal":{"name":"Inmunologia (Barcelona, Spain : 1987)","volume":"32 1","pages":"Pages 25-34"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.inmuno.2012.08.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54642578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Obtención de células madre mesenquimales a partir de cordones umbilicales procedentes de un programa altruista de donación de sangre de cordón 从利他的脐带献血计划中获得脐带间充质干细胞

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2013-01-01 DOI: 10.1016/j.inmuno.2012.11.002

Aina Arbós , Francesca Nicolau , Marta Quetglas , Joana Maria Ramis , Marta Monjo , Josep Muncunill , Javier Calvo , Antoni Gayà

{"title":"Obtención de células madre mesenquimales a partir de cordones umbilicales procedentes de un programa altruista de donación de sangre de cordón","authors":"Aina Arbós , Francesca Nicolau , Marta Quetglas , Joana Maria Ramis , Marta Monjo , Josep Muncunill , Javier Calvo , Antoni Gayà","doi":"10.1016/j.inmuno.2012.11.002","DOIUrl":"10.1016/j.inmuno.2012.11.002","url":null,"abstract":"<div><h3>Introduction</h3><p>Mesenchymal stem cells (MSC) offer a great potential for regenerative medicine due to their unique properties of self-renewal, high plasticity and modulation of immune response. Although the original source of MSC has been bone marrow, there is a clear need for a source of MSC as an “off-the-shelf” product for quick and effective treatment. One of the first candidates was the umbilical cord blood (UCB) but the poor recovery discourages its use.</p></div><div><h3>Material and methods</h3><p>In the present paper, we tested umbilical cord (UC) tissue as a source for obtaining MSC. All the samples were obtained from donations included in our UCB altruistic procurement program.</p></div><div><h3>Results</h3><p>Our results showed that is possible to obtain MSC from UC (hUC-MSC) with a 100% success rate by using a combination of mechanical fragmentation and enzymatic digestion. The MSC thus obtained show a phenotype very similar to that observed in the MSC of bone marrow origin: CD45<sup>−</sup>CD31<sup>−</sup>CD34<sup>−</sup>HLA-DR-CD<sup>−</sup>105<sup>+</sup>CD90<sup>+</sup>CD73<sup>+</sup>. In addition, we have demonstrated that hUC-MSC, like the MSC from bone marrow, are able to differentiate into osteoblasts and adipocytes, and also exert a suppressive effect on the proliferative capacity of peripheral lymphocytes stimulated with phytohaemagglutinin.</p></div><div><h3>Conclusions</h3><p>We conclude that it is possible to implement a structured program of MSC derivation by using the same logistics that are used to obtain UCB. This opens the way to developing a biobank of human MSC that could be useful for research and clinical use.</p></div>","PeriodicalId":88896,"journal":{"name":"Inmunologia (Barcelona, Spain : 1987)","volume":"32 1","pages":"Pages 3-11"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.inmuno.2012.11.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54642692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Dr. Gregory Winter y Dr. Richard A. Lerner, Premios Príncipe de Asturias de Investigación Científica y Técnica 2012 格雷戈里·温特博士和理查德·a·勒纳博士，2012年Príncipe阿斯图里亚斯Investigación Científica与taca的Premios

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2012-10-01 DOI: 10.1016/j.inmuno.2012.09.001

África González-Fernández , Luis Álvarez-Vallina

引用次数: 0

Ha llegado el momento… 是时候了……

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2012-10-01 DOI: 10.1016/j.inmuno.2012.11.001

Francesc E. Borràs

引用次数: 2

Raman spectroscopic study of transfected HEK293T cells 转染HEK293T细胞的拉曼光谱研究

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2012-10-01 DOI: 10.1016/j.inmuno.2012.08.004

Joel Joshi , Santiago Sanchez-Cortes , Narahari V. Joshi , Enrique Aguado , Francisco J. Garcia-Cozar

引用次数: 0

Bisulfite genomic sequencing to uncover variability in DNA methylation: Optimized protocol applied to human T cell differentiation genes 亚硫酸氢盐基因组测序揭示DNA甲基化变异性:应用于人类T细胞分化基因的优化方案

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2012-10-01 DOI: 10.1016/j.inmuno.2012.09.002

Paula A. Correa , Ricardo Pujol-Borrell , Roger Colobran

{"title":"Bisulfite genomic sequencing to uncover variability in DNA methylation: Optimized protocol applied to human T cell differentiation genes","authors":"Paula A. Correa , Ricardo Pujol-Borrell , Roger Colobran","doi":"10.1016/j.inmuno.2012.09.002","DOIUrl":"10.1016/j.inmuno.2012.09.002","url":null,"abstract":"<div><p><span><span><span>DNA methylation, which most commonly occurs at the C5 position of </span>cytosines<span> within CpG dinucleotides, is one of several </span></span>epigenetic mechanisms<span><span><span> that cells use to control gene expression. The importance of DNA methylation in a variety of </span>biological processes<span><span><span> (i.e., embryonic development, cellular proliferation and differentiation, chromosome stability) has led to a demand for a precise and efficient method to determine the exact DNA methylation status. </span>Bisulfite </span>genomic sequencing is regarded as a gold-standard technology for detection of DNA methylation as it provides a qualitative, quantitative and efficient approach to identify 5-methylcytosine at single base-pair resolution. To optimize the final results of the bisulfite genomic sequencing protocol, numerous modifications have been explored and have significantly improved the sensitivity and accuracy of this procedure. The aim of this methodological report is to give an overview of the bisulfite genomic sequencing protocol, discussing the critical methodological aspects. Since we are interested in studying the methylation status of specific genes involved in </span></span>T cell development, we applied the bisulfite genomic sequencing to the study of the </span></span><span><em>CD8A</em></span><span> T cell co-receptor gene to determine whether the CGIs of this gene were subjected to methylation in different types of tissues. The results show that </span><em>CD8A</em> gene is differentially methylated depending on the tissue. In conclusion, we described a bisulfite genomic sequencing protocol that can be successfully used for the quantitative analysis of CpG island methylation of specific genes.</p></div>","PeriodicalId":88896,"journal":{"name":"Inmunologia (Barcelona, Spain : 1987)","volume":"31 4","pages":"Pages 97-105"},"PeriodicalIF":0.0,"publicationDate":"2012-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.inmuno.2012.09.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54642609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1