Inmunologia (Barcelona, Spain : 1987)最新文献_第10页

Aplicación de la probabilidad y la entropía a la proteína EBA-140. Caracterización matemática de péptidos de alta unión 概率和熵在EBA-140蛋白中的应用。高结合肽的数学表征

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2009-04-01 DOI: 10.1016/S0213-9626(09)70028-9

Javier Rodríguez , Catalina Correa , Signed Prieto , Germán Puerta , Sarith Vitery , Pedro Bernal , Yolanda Soracipa , David Botero

{"title":"Aplicación de la probabilidad y la entropía a la proteína EBA-140. Caracterización matemática de péptidos de alta unión","authors":"Javier Rodríguez , Catalina Correa , Signed Prieto , Germán Puerta , Sarith Vitery , Pedro Bernal , Yolanda Soracipa , David Botero","doi":"10.1016/S0213-9626(09)70028-9","DOIUrl":"10.1016/S0213-9626(09)70028-9","url":null,"abstract":"<div><p>One of the <em>Plasmodium falciparum</em> merozoite proteins, EBA-140, also known as BAEBL or PfEBP-2, shares structural features and homology with EBA-175 and EBA-181. Studies on the sub-cellular localization suggest a micronem localization.</p><p>A probability space was built where the possibility of appearance by position for each of the 20 amino acids in EBA-140 protein 20-mer peptides based on 6 high-binding previously described sequences was quantified. Then, the probability, the addition of probability and the Entropy for 61 EBA-140 protein 20-mer sequences were calculated to mathematically characterize the high-binding peptides and the non-high-binding peptides. Additionally, the same measures for theoretical peptide analogs were made, in which calculations were made after those amino acids tested experimentally as critical were substituted by Glycine.</p><p>The probability values, probability summation and Entropy for the experimentally verified high-binding sequences, vary between the ranges associated to the binding macrostate, while every value for the nonhigh- binding peptides are outside of the binding macrostate range. The probability values, probability summation and Entropy differentiate the high-binding peptides from the low-binding, making a right guess in 100% of the cases studied according to experimental studies.</p><p>The binding phenomenon of the studied protein has an underlying order, which is objectively and reproductively characterizable starting from probability laws and Entropy.</p></div>","PeriodicalId":88896,"journal":{"name":"Inmunologia (Barcelona, Spain : 1987)","volume":"28 2","pages":"Pages 65-73"},"PeriodicalIF":0.0,"publicationDate":"2009-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0213-9626(09)70028-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56138246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Cómo promover y facilitar el diagnóstico precoz de la infección por el VIH-1 如何促进和促进hiv -1感染的早期诊断

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2009-04-01 DOI: 10.1016/S0213-9626(09)70032-0

Jose M. Gatell , Daniel Zulaica , Jorge Del Romero , Teresa Robledo , representación de la Plataforma “VIH en España 2009”

{"title":"Cómo promover y facilitar el diagnóstico precoz de la infección por el VIH-1","authors":"Jose M. Gatell , Daniel Zulaica , Jorge Del Romero , Teresa Robledo , representación de la Plataforma “VIH en España 2009”","doi":"10.1016/S0213-9626(09)70032-0","DOIUrl":"10.1016/S0213-9626(09)70032-0","url":null,"abstract":"<div><p>Human Inmunodeficiency Virus type 1 infection (HIV-1) was reported in 1981 and quickly became a pandemic and the most frequent cause of mortality among people between 20 and 40 years. The introduction of Highly Active Antiretroviral Therapy (HAART) back in 1996 was associated with a dramatic reduction in mortality and the majority of HIV-1 infected patients have now a life expectancy approaching the general population of similar age and gender with a relatively good quality of life. Conversely, most of the preventive policies and measures have failed and what may be even worse is that in Western Europe for every two known HIV cases there is one unknown (33%). This 33% of not yet diagnosed HIV infections contribute to an excess of HIV-1 transmissions (60–70% of the total). Moreover, these patients with a late diagnosis will not benefit from the advantages of an early treatment.</p><p>In the USA, to promote early diagnosis of the HIV-1 infection the recommendation is universal testing each time a sexually active person contacts with the health system. In Europe, initiatives like “HIV in Europe” or “HIV in Spain 2009” recommended a screening policy based on the presence of indicator situations or events where the estimated prevalence of HIV-1 infection is above 1%.</p></div>","PeriodicalId":88896,"journal":{"name":"Inmunologia (Barcelona, Spain : 1987)","volume":"28 2","pages":"Pages 101-104"},"PeriodicalIF":0.0,"publicationDate":"2009-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0213-9626(09)70032-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"104468741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Análisis comparativo de tres métodos de ELISA frente a Inmunodot para la determinación de antígenos extraíbles del núcleo (ENAs) 三种ELISA法与免疫dot法测定核可提取抗原(ENAs)的比较分析

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2009-01-01 DOI: 10.1016/S0213-9626(09)70022-8

Natividad López Riquelme , Mª Dolores Dosda González , Francisco Antonio Ramírez Garrido , Obdulia Noguera Moya , Antonia Espasa Sempere , Daniel Cañas Bello

{"title":"Análisis comparativo de tres métodos de ELISA frente a Inmunodot para la determinación de antígenos extraíbles del núcleo (ENAs)","authors":"Natividad López Riquelme , Mª Dolores Dosda González , Francisco Antonio Ramírez Garrido , Obdulia Noguera Moya , Antonia Espasa Sempere , Daniel Cañas Bello","doi":"10.1016/S0213-9626(09)70022-8","DOIUrl":"10.1016/S0213-9626(09)70022-8","url":null,"abstract":"<div><p>The detection and the specificity of the autoantibodies to extractable nuclear antigens (ENAs) play a critical role in the development of the diagnosis of certain autoimmune diseases. There are several methods to detect these autoantibodies, and enzyme-linked immunosorbent assay (ELISA) or the simple dot-blot are the most frequently used.</p><p>Here, we evaluate four methods to detect ENAs, namely an Immunodot test along with three different ELISA tests. Twenty six samples of patients were analysed. Antibodies to nuclear antigens (ANAS) were determined by indirect immunofluorescence (IFI), whereas antibodies to different ENAs (Ro, La, Sm-RNP, Sm, Scl-70 and Jo-1) were determined by simple dot-blot and by the three ELISA tests. In 27% of the cases all the techniques yielded similar results, and were related to the clinical symptoms of the patient. The concordance among the different ELISA techniques tested was 88% for ELISA 1 vs 2, 73% for ELISA 2 vs 3, and 73% for ELISA 2 vs 3. The concordance of the ELISA methods with the Immunodot was 38%, 42% and 27% for ELISA 1, 2, and 3, respectively.</p><p>The ELISA techniques obtained a higher percentage of concordance among them than with Immunodot and, moreover, they related better to the clinical symptoms and ANA pattern of the patients. The results also show that the presence or the specific type of ENA autoantibody does not always have a relation to the clinical status of the patient, so that the laboratory and the clinic must be conscious of the sensitivity and specificity limits of each method used in the clinical laboratory.</p></div>","PeriodicalId":88896,"journal":{"name":"Inmunologia (Barcelona, Spain : 1987)","volume":"28 1","pages":"Pages 7-11"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0213-9626(09)70022-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56137855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eph and ephrin: Key molecules for the organization and function of the thymus gland ephh和ephrin:胸腺组织和功能的关键分子

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2009-01-01 DOI: 10.1016/S0213-9626(09)70024-1

Juan José Muñoz, David Alfaro, Javier García-Ceca, Teresa Cejalvo, Marco A. Stimamiglio, Eva Jiménez, Agustín G. Zapata

{"title":"Eph and ephrin: Key molecules for the organization and function of the thymus gland","authors":"Juan José Muñoz, David Alfaro, Javier García-Ceca, Teresa Cejalvo, Marco A. Stimamiglio, Eva Jiménez, Agustín G. Zapata","doi":"10.1016/S0213-9626(09)70024-1","DOIUrl":"10.1016/S0213-9626(09)70024-1","url":null,"abstract":"<div><p>Eph are the largest family of protein tyrosine kinases, which are described in most cell types. Together with their ligands, ephrins, Eph participate in the organogenesis of many tissues mediating numerous processes, such as cell positioning and cell migration, which are key for the functioning of the thymus: a primary lymphoid organ involved in T-cell maturation. In the present study, we review available data on the role played by these families of molecules in the biology of the thymus gland. Most Eph and ephrin are expressed in adult and fetal thymus, frequently in both thymocytes and thymic stromal cells. They appear to play an essential role in governing thymus size through the control of survival of thymocytes and thymic epithelial cells (TEC). Furthermore, studies <em>in vivo</em> and <em>in vitro</em> demonstrate that altered Eph/ephrin signalling results in specific thymus phenotypes and conclude that the balance of Eph/ephrin signals finally determine the pattern of maturation/differentiation of thymocytes and TEC. Indeed, the role of Eph/ephrin in thymus function appears early in ontogeny. In this regard, several results emphasize their relevance in key processes for thymus organization, such as the recruitment of lymphoid progenitors to the thymic primordium, the branching pattern of thymus epithelium and the positioning of thymocytes and TEC in the growing gland. Some of the phenotypic alterations observed in the thymus of Eph/ephrin deficient mice are reflected in the peripheral lymphoid organs, but there is no evidence for alterations in the function of their immune systems.</p></div>","PeriodicalId":88896,"journal":{"name":"Inmunologia (Barcelona, Spain : 1987)","volume":"28 1","pages":"Pages 19-31"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0213-9626(09)70024-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56137933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

La gripe o la influencia de las estrellas 流感或星星的影响

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2009-01-01 DOI: 10.1016/S0213-9626(09)70026-5

María Montoya

引用次数: 0

El 35 Aniversario de la Sociedad Española de Inmunología (29 de Abril, Día Global de la Inmunología) 西班牙免疫学会成立35周年(4月29日，全球免疫日)

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2009-01-01 DOI: 10.1016/S0213-9626(09)70027-7

Julia Sequí Navarro , Jordi Vives Puiggros , José María Rojo , Carmen Gutiérrez Martín

引用次数: 0

The Th17 lineage: Answers to some immunological questions Th17谱系:一些免疫学问题的答案

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2009-01-01 DOI: 10.1016/S0213-9626(09)70025-3

Coral González-García, Francisco M. Martín-Saavedra, Alicia Ballester, Sara Ballester

{"title":"The Th17 lineage: Answers to some immunological questions","authors":"Coral González-García, Francisco M. Martín-Saavedra, Alicia Ballester, Sara Ballester","doi":"10.1016/S0213-9626(09)70025-3","DOIUrl":"10.1016/S0213-9626(09)70025-3","url":null,"abstract":"<div><p>In recent years the function and developmental pathway for the T helper subset specialized in IL-17 production (Th17) have been exhaustively studied. This lineage of effector cells plays a decisive role in the immune response to infectious agents, as well as in immunopathologies. Similar to the Th1 and Th2 subsets, the Th17 definition is orchestrated by specific cytokines and transcription factors. A combination of TGF-β plus IL-6, and the transcription factors RORγt, RORα and Stat3 are essential for Th17 commitment. IL-23 plays a key role in the stabilization of the phenotype and in the promotion of the pathogenic activity of IL-17-producer cells. The IL-21 cytokine produced by Th17 cells participates in a feedback mechanism to favour this phenotype, while IL-27, IL-4, IFN-γ, IL-25 and IL-2 cytokines limit the Th17 response. CD4<sup>+</sup>CD25<sup>+</sup>Foxp3<sup>+</sup> regulator cells (Treg) follow a development pathway divergent to Th17 establishment, although both alternatives are governed by TGF-β that directs the fate of naïve CD4<sup>+</sup> cells to each of these mutually exclusive T cell subsets depending on the presence of IL-6. Furthermore, recent data indicate that preestablished Treg cells can switch its genetic program to become IL-17-producer cells. In this review we summarize and discuss the current available data about the biology of Th17 cells.</p></div>","PeriodicalId":88896,"journal":{"name":"Inmunologia (Barcelona, Spain : 1987)","volume":"28 1","pages":"Pages 32-45"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0213-9626(09)70025-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56137964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Teoría de unión al HLA clase II: teoría de probabilidad, combinatoria y entropía aplicadas a secuencias peptídicas HLA结合理论II类:应用于肽序列的概率论、组合论和熵论

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2008-10-01 DOI: 10.1016/S0213-9626(08)70064-7

Javier Rodríguez

引用次数: 52

Polly Matzinger's “danger model” finds its predicted danger-denoting self moieties Polly Matzinger的“危险模型”找到了它所预测的代表危险的自我部分

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2008-10-01 DOI: 10.1016/S0213-9626(08)70068-4

Asis Palazón, Juan Dubrot, Iván Martinez-Forero, Ana Rouzaut-Subirá, Carmen Ochoa, José Luis Perez-Gracia, Sandra Hervás-Stubbs, Ignacio Melero

{"title":"Polly Matzinger's “danger model” finds its predicted danger-denoting self moieties","authors":"Asis Palazón, Juan Dubrot, Iván Martinez-Forero, Ana Rouzaut-Subirá, Carmen Ochoa, José Luis Perez-Gracia, Sandra Hervás-Stubbs, Ignacio Melero","doi":"10.1016/S0213-9626(08)70068-4","DOIUrl":"10.1016/S0213-9626(08)70068-4","url":null,"abstract":"<div><p><span><span><span>The “danger theory” is a set of postulates formally proposed by Polly Matzinger fifteen years ago. As a theory, it explains how it is possible that immune responses take place without infection. It provokingly proposed that the immune systems have not evolved for self/non-self discrimination, but to respond against what is causing tissue damage. The proposal of the “danger model” coincided in time with the discovery of the immune potentiating effects of microbial molecular patterns. Charles Janeway et al. proposed that infection would be detected by innate receptors for microbiological biomolecules that are either absent or different in mammals. These agents were found to stimulate antigen presenting cells in such a way that would provide </span>T lymphocytes<span><span><span> with appropriate costimulatory molecules that critically complement the signals raised by antigen recognition. This was considered absolutely critical to ignite and sustain immune responses. The danger theory predicted the existence of endogenous molecules released or modified by danger that would act in a similar fashion to the microbial molecular patterns on dendritic cell costimulatory functions. Recent evidence points to various molecules capable of sounding the alarm in aseptic conditions. These include: the nuclear protein HBGM-1, </span>uric acid, Interferon-α, chaperones of the </span>heat shock protein family, alternatively spliced domains of </span></span>fibronectin<span>, and self nucleic acids<span>. Some of these agents act through the same Toll like receptors involved in microbial pattern recognition. Identification of these mechanisms provides molecular support for the danger theory and has an extraordinary importance for tumor and </span></span></span>transplantation immunology.</p></div>","PeriodicalId":88896,"journal":{"name":"Inmunologia (Barcelona, Spain : 1987)","volume":"27 4","pages":"Pages 205-211"},"PeriodicalIF":0.0,"publicationDate":"2008-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0213-9626(08)70068-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56137745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Roitt. Inmunología. Fundamentos (11a edición) Roitt。免疫学。基础(第11版)

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2008-10-01 DOI: 10.1016/S0213-9626(08)70069-6

Peter J. Delves, Seamus J. Martin, Dennis R. Burton, Ivan M. Roitt, Pedro Aparicio Alonso

引用次数: 0