Inmunologia (Barcelona, Spain : 1987)最新文献_第10页

PCR-based microsatellite analysis to accelerate diabetogenic genetic background acquisition in transgenic mice 基于pcr的微卫星分析加速转基因小鼠的糖尿病遗传背景获取

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2011-01-01 DOI: 10.1016/S0213-9626(11)70008-7

Rosa M. Ampudia , Aurora Alba , Raquel Planas , Irma Pujol-Autonell , Concepción Mora , Joan Verdaguer , Marta Vives-Pi

{"title":"PCR-based microsatellite analysis to accelerate diabetogenic genetic background acquisition in transgenic mice","authors":"Rosa M. Ampudia , Aurora Alba , Raquel Planas , Irma Pujol-Autonell , Concepción Mora , Joan Verdaguer , Marta Vives-Pi","doi":"10.1016/S0213-9626(11)70008-7","DOIUrl":"10.1016/S0213-9626(11)70008-7","url":null,"abstract":"<div>The Non-obese diabetic (NOD) mice exhibit a susceptibility to spontaneous development of autoimmune diabetes and is the most widely used experimental model for the study of the disease. The NOD strain was established by inbreeding in 1980. This model has a MHC-matched diabetes resistant homologous, NOR/Lt mice, an insulitis-resistant and diabetes-free strain produced from an isolated genetic contamination within a NOD/Lt line. To evaluate the role of transgenes, transgenic mice can be generated in CD-1 mice for technical advantages and then backcrossed to inbred strains. To obtain transgenic mice in NOD or NOR background starting from CD-1, at least 20 backcrosses are required, spending more than two years in the process.Nucleotide repeats (microsatellites) mapped to specific locations on each chromosome are used to evaluate genomic polymorphism. From 23 microsatellites we selected eleven that were variant in PCR amplimer size between CD-1 colony and NOD or NOR strains. We used these microsatellites to identify individuals that were used for backcrossing, thus accelerating the acquisition of a new genetic background. Results yield a defined analysis of the genome in question and profiles were compared to detect genetic variation among individuals. After the selection of mice for backcrossing at the third generation, the 11 specific markers were acquired at the 5th generation and maintained to the 10th generation. Diabetes incidence and insulitis score correlated with the acquisition of genetic background, demonstrating that using this strategy, 5–6 crosses are enough to obtain the genotype of interest, shortening the process in more than one year and a half.</div>","PeriodicalId":88896,"journal":{"name":"Inmunologia (Barcelona, Spain : 1987)","volume":"30 1","pages":"Pages 2-7"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0213-9626(11)70008-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56139703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Informe técnico del IX Taller de Citometría de Flujo: inmunofenotipo de leucemias 第九届流式细胞术研讨会技术报告:白血病免疫表型

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2011-01-01 DOI: 10.1016/S0213-9626(11)70010-5

Berta Sánchez Sánchez

引用次数: 0

César Milstein: 35 años de anticuerpos monoclonales cesar Milstein: 35年的单克隆抗体研究

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2011-01-01 DOI: 10.1016/S0213-9626(11)70012-9

África González-Fernández , Fernando Díaz de Espada

引用次数: 2

Patrones diferenciales de distribución de los alelos y genotipos HLA-DPA1* en asociación con leucemias linfoides agudas y leucemias mieloides crónicas en pacientes del estado Zulia, Venezuela 委内瑞拉Zulia州患者急性淋巴细胞白血病和慢性髓细胞白血病相关HLA-DPA1*等位基因分布和基因型的差异模式

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2010-10-01 DOI: 10.1016/S0213-9626(10)70002-0

Miriam Echeverría , Sergio Rivera Pirela , Georgina Márquez , Zuhey Carrillo , Yennis Parra , Carmen Cecilia Villalobos

{"title":"Patrones diferenciales de distribución de los alelos y genotipos HLA-DPA1* en asociación con leucemias linfoides agudas y leucemias mieloides crónicas en pacientes del estado Zulia, Venezuela","authors":"Miriam Echeverría , Sergio Rivera Pirela , Georgina Márquez , Zuhey Carrillo , Yennis Parra , Carmen Cecilia Villalobos","doi":"10.1016/S0213-9626(10)70002-0","DOIUrl":"10.1016/S0213-9626(10)70002-0","url":null,"abstract":"<div>Little is known about the relevance of the polymorphisms in the function of HLA-DP. Acute lymphoblastic leukemia (ALL) is the most frequent cancer in children and adolescents in Zulia population. Chronic myeloid leukemia (CML) is similar in adolescents, young adults and mild adults. The epidemiologic studies suggest the presence of several factors related to the susceptibility. Forty-eight patients with ALL and 48 with CML, were compared with 48 controls from The Blood Bank of Zulia State, Venezuela, all of them unrelated racially mestizos. To evaluate the positive and negative associations between HLA allele and leukemias, the HLA-DPA1 locus and HLA-DPA1*01,*02,*03 and *04 alleles were studied using PCR Olerup SSPTM (Genovision). HLA-DPA1*01:05 (RR = 3.65; P ≤ .05) and DPA1*01:06 (RR = 13.88; P ≤ .05) alleles showed a positive association with ALL. Furthermore, HLA-DPA1*01:03:01-01:03:02 (RR = 0.46; P ≤ .05), DPA1*01:07 (RR = 10; P ≤ .05) and DPA1*02:01:01-02:01:06 (RR = 0.29; P ≤ .05) were negatively associated with ALL. Moreover, HLA-DPA1*01:05 (RR = 0.08; P ≤ .05), DPA1*01:08 (RR = 0.06; P ≤ .05) and DPA1*01:09 (RR = 0.14; P ≤ .05) showed a negative association with CML. Curiously, the genotype HLA-DPA1*01:03:01-01:03:02/02:01:01-02:02:06 showed a frequency of 40.4% in controls, 8.5% in ALL patients and 64.6% in CML patients. These marked differences in the frequency of distribution of HLA-DPA1* alleles and genotypes in CML and ALL patients, probably reveals a important pathogenic differences for the two types of leukemia.</div>","PeriodicalId":88896,"journal":{"name":"Inmunologia (Barcelona, Spain : 1987)","volume":"29 4","pages":"Pages 115-118"},"PeriodicalIF":0.0,"publicationDate":"2010-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0213-9626(10)70002-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56138741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Stiff person syndrome and cerebellar ataxia associated with glutamic acid decarboxylase antibodies and type 1 diabetes: What is the link between neurological diseases and autoimmunity to the beta cell? 与谷氨酸脱羧酶抗体和1型糖尿病相关的僵硬者综合征和小脑性共济失调:神经系统疾病与β细胞自身免疫之间的联系是什么?

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2010-10-01 DOI: 10.1016/S0213-9626(10)70003-2

Marta Vives-Pi , Lidia Sabater

引用次数: 0

Inmunorregulación: un nuevo paradigma terapéutico 免疫调节:一种新的治疗范式

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2010-10-01 DOI: 10.1016/S0213-9626(10)70005-6

Irene Pradas Barriga, María Luisa del Río González, José Ignacio Rodríguez Barbosa

引用次数: 0

Biological aspects of human plasmacytoid dendritic cells and their leukemic counterparts; similarities and differences 人浆细胞样树突状细胞及其白血病对应物的生物学特性异同

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2010-10-01 DOI: 10.1016/S0213-9626(10)70004-4

Laura Bover , Mar Naranjo-Gómez , Shino Hanabuchi , Begoña Pérez-Cabezas , Francesc E. Borràs

引用次数: 0

Influenza outbreak, a year after the pandemic, what have we learned? 流感大流行一年后爆发，我们学到了什么?

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2010-10-01 DOI: 10.1016/S0213-9626(10)70006-8

María Montoya , Francesc E. Borràs

引用次数: 0

Ilusión y determinación 幻想与决心

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2010-10-01 DOI: 10.1016/S0213-9626(10)70001-9

Francesc E. Borràs

引用次数: 1

Predicción de unión de péptidos de MSP-1 y EBA-140 de Plasmodium falciparum al HLA clase II 恶性疟原虫MSP-1和EBA-140肽与HLA II类肽结合的预测

Inmunologia (Barcelona, Spain : 1987) Pub Date : 2010-07-01 DOI: 10.1016/S0213-9626(10)70016-0

Javier Rodríguez, Pedro Bernal, Luisa Álvarez, Sandra Pabón, Sandra Ibáñez, Nanyid Chapuel, Héctor Pérez, Alexandra Correa, Luis Carlos Salazar, Raúl Walteros

{"title":"Predicción de unión de péptidos de MSP-1 y EBA-140 de Plasmodium falciparum al HLA clase II","authors":"Javier Rodríguez, Pedro Bernal, Luisa Álvarez, Sandra Pabón, Sandra Ibáñez, Nanyid Chapuel, Héctor Pérez, Alexandra Correa, Luis Carlos Salazar, Raúl Walteros","doi":"10.1016/S0213-9626(10)70016-0","DOIUrl":"10.1016/S0213-9626(10)70016-0","url":null,"abstract":"<div>The MSP-1 merozoite surface protein is related to the invasion phenomenon of malaria to red cells, while the EBA-140 antigen protein interacts with the sialoglycoproteins on the surface of erythrocytes. The identification of peptides from both proteins that bind to HLA class II has a particular importance for the development of vaccines.In the present work, a predictive binding to HLA class II theory, based on S/k proportion of entropy was applied, for the prediction of proteins MSP-1 and EBA-140 binding phenomenon to the totality of 20 amino acid sequences of both molecules. The probability, combinatory and entropy values were calculated for 948 and 732 nonamer overlapping sequences of MSP-1 and EBA-140, respectively. Three theoretical proteins of 500 aminoacids of lenght each were computationally built in order to apply the developed binding theory to all their nonamer overlapping peptides.It was predicted that for the two studied merozoite proteins, 298 sequences are related to the binding macrostate and 1409 to the not-binding macrostate. The developed theoretical prediction can facilitate the selection of peptides during the process of vaccine development. For the three theoretical proteins built, it was found that 111, 84 and 72 are predicted as included into the binding macrostate, while sequences 381, 408, and 420 are predicted as related to the not-binding macrostate.The predictions are an evidence of a physical and mathematical order in antigen presentation, which can be useful to the development of vaccines.</div>","PeriodicalId":88896,"journal":{"name":"Inmunologia (Barcelona, Spain : 1987)","volume":"29 3","pages":"Pages 91-99"},"PeriodicalIF":0.0,"publicationDate":"2010-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0213-9626(10)70016-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56139531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15