Signal transduction最新文献_第2页

Clustering of membrane proteins in the pre-stimulation stage is required for signal transduction: a computer analysis 在刺激前阶段膜蛋白的聚类是信号转导所必需的:计算机分析

Signal transduction Pub Date : 2007-08-01 DOI: 10.1002/SITA.200790020

Hiroshi Kobayashi, R. Azuma, A. Konagaya

{"title":"Clustering of membrane proteins in the pre-stimulation stage is required for signal transduction: a computer analysis","authors":"Hiroshi Kobayashi, R. Azuma, A. Konagaya","doi":"10.1002/SITA.200790020","DOIUrl":"https://doi.org/10.1002/SITA.200790020","url":null,"abstract":"The physiological significance of membrane protein clustering for signal transduction was examined theoretically using a Monte Carlo computer simulation. Simulation results revealed that pre-stimulation clustering of membrane proteins enhanced signal transduction. Membrane protein clustering induced by the binding of external stimuli provided no kinetic advantage in terms of formation rate or maximum quantity of active membrane receptor complexes. These data suggested that membrane proteins associate weakly in the clustering areas of non-stimulated cells, and that their association is strengthened upon binding of extracellular stimuli to the membrane receptor. Additionally, the number of cytosolic proteins recruited to membrane receptor complexes was not increased by the membrane complex clustering, except when cytosolic signal proteins were localized to a narrow area such as a tunnel that ran from the membrane cluster to the nucleus. Simulations were carried out on a conventional personal computer under Windows XP or 2000 operating systems. Since neither special computing hardware nor special training is required, our simulation procedure could be easily adapted for kinetic analysis of any signal transduction pathway.","PeriodicalId":88702,"journal":{"name":"Signal transduction","volume":"110 1","pages":"329-339"},"PeriodicalIF":0.0,"publicationDate":"2007-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/SITA.200790020","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50951491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The stoichiometry of the T cell antigen receptor and its implications for the signal transduction mechanism T细胞抗原受体的化学计量学及其对信号转导机制的影响

Signal transduction Pub Date : 2007-08-01 DOI: 10.1002/SITA.200600123

W. Schamel

引用次数: 2

The pre‐B cell receptor and its ligands – it takes two to tango 前B细胞受体和它的配体-需要两个才能探戈

Signal transduction Pub Date : 2007-08-01 DOI: 10.1002/SITA.200500055

Harald Bradl, Christian Vettermann, W. Schuh, S. Meister, H. Jäck

{"title":"The pre‐B cell receptor and its ligands – it takes two to tango","authors":"Harald Bradl, Christian Vettermann, W. Schuh, S. Meister, H. Jäck","doi":"10.1002/SITA.200500055","DOIUrl":"https://doi.org/10.1002/SITA.200500055","url":null,"abstract":"DFG Training Program GK592, Nikolaus Fiebiger Center for Molecular Medicine, University ofErlangen and Nurnberg, GermanyThe development of early precursor B cells is governed by the surface-bound pre-B cell receptorconsisting of the immunoglobulin µ heavy chain, the surrogate light chain components λ5andVpreB, and the signal transducing subunits immunglobulin α/immunglobulin β. The pre-B cell re-ceptor controls clonal expansion, survival and efficient differentiation of functional B lymphoid pre-cursors; however, it is still controversial how signals from this receptor are initiated. Recent studieswith Abelson murine leukemia virus (Abl-MuLV)-transformed pre-B cell lines suggest that the N-terminal non-immunoglobulin portion of λ5, the so-called unique tail, is required to initiate cell-autonomous signals by mediating self-aggregation of the pre-B cell receptor (pre-BCR). Strikinglyhowever, the λ5 unique tail also controls the interaction with two different groups of stroma cell-derived pre-BCR ligands, namely heparan sulfate glycosaminoglycans and surface-associated ga-lectin-1. Even though these findings are not mutually exclusive, they refresh the discussion aboutpotential modes of pre-BCR signal initiation. In this review, we discuss recent key findings andpropose an integrative model for ligand dependent and independent initiation of pre-BCR signalsduring selection of functional B cell precursors.","PeriodicalId":88702,"journal":{"name":"Signal transduction","volume":"7 1","pages":"299-310"},"PeriodicalIF":0.0,"publicationDate":"2007-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/SITA.200500055","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50950316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Signal transduction in vestibular adaptation to microgravity – A still unsolved problem 前庭适应微重力的信号转导——一个尚未解决的问题

Signal transduction Pub Date : 2007-06-01 DOI: 10.1002/SITA.200600107

E. Horn

引用次数: 4

Dlk/ZIP kinase, a novel Ser/Thr‐specific protein kinase with multiple functions Dlk/ZIP激酶是一种具有多种功能的新型丝氨酸/苏氨酸特异性蛋白激酶

Signal transduction Pub Date : 2007-06-01 DOI: 10.1002/SITA.200600112

K. Scheidtmann

引用次数: 6

Serotonin 1A receptor coupling to NF‐κB studied using inducible receptor expression in hippocampal neuron‐derived cells 利用诱导受体在海马神经元源性细胞中的表达研究血清素1A受体与NF - κB的偶联

Signal transduction Pub Date : 2007-06-01 DOI: 10.1002/SITA.200600087

T. Sobocki, I. Ray, P. Cano‐Sánchez, Probal Banerjee, E. Azmitia

{"title":"Serotonin 1A receptor coupling to NF‐κB studied using inducible receptor expression in hippocampal neuron‐derived cells","authors":"T. Sobocki, I. Ray, P. Cano‐Sánchez, Probal Banerjee, E. Azmitia","doi":"10.1002/SITA.200600087","DOIUrl":"https://doi.org/10.1002/SITA.200600087","url":null,"abstract":"The mechanism of serotonin 1A receptor (5-HT1A-R) mediated activation of NF-κB has been studied in non-neural cells, but this pathway has not been elucidated in neuronal cells. We have used inducible expression of the 5-HT1A-R in the hippocampal neuron-derived HN2 cells to analyze the coupling of this receptor to NF-κB. A construct harboring luciferase cDNA driven by a minimal promoter under the control of an NF-κB-specific enhancer element was transfected into these 5-HT1A-R-expressing HN2 cells. Using luciferase expression in the transfected cells, we have studied 5-HT1A-R agonist evoked activation of NF-κB. Inhibition of either mitogen activated protein kinase (MAPK) pathway with PD98059 or protein kinase C (PKC) with GFX caused elevation of the basal level of NF-κB activity but did not affect 5-HT1A-R mediated activation of NF-κB. Furthermore, neither the basal level of NF-κB nor its activation by a 5-HT1A-R agonist was altered by dibutyrylcAMP. Thus, the MAPK pathway and PKC cause inhibition of the basal NF-κB activity and the 5-HT1A-R-linked NF-κB activation does not require MAPK, PKC, and cAMP. Intriguingly, Western blot analysis showed that 5-HT1A-R mediates activation of both CaMKII and PI-3K. This 5-HT1A-R-evoked the stimulation of CaMKII was reversed in the presence of a PI-3K inhibitor. Therefore, the likely mechanism of 5-HT1A-R mediated induction of NF-κB in neuronal cells involves activation of PI-3K upstream of CaMKII. This reveals a novel pathway that could be crucial in the functional activity of brain neurons.","PeriodicalId":88702,"journal":{"name":"Signal transduction","volume":"67 2","pages":"260-269"},"PeriodicalIF":0.0,"publicationDate":"2007-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/SITA.200600087","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50950853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

p90 Ribosomal S6 kinases‐ eclectic members of the human kinome p90核糖体S6激酶-人类激酶的折衷成员

Signal transduction Pub Date : 2007-06-01 DOI: 10.1002/SITA.200600091

Kwok Y. Lee, P. Bignone, T. Ganesan

引用次数: 3

The generation of locomotory forces in the spontaneous and norepinephrine‐induced migration of carcinoma cells 在自发和去甲肾上腺素诱导的癌细胞迁移中产生的运动力

Signal transduction Pub Date : 2007-06-01 DOI: 10.1002/SITA.200600088

C. Strell, F. Entschladen, P. Bastian, B. Niggemann, K. Zänker, K. Lang

{"title":"The generation of locomotory forces in the spontaneous and norepinephrine‐induced migration of carcinoma cells","authors":"C. Strell, F. Entschladen, P. Bastian, B. Niggemann, K. Zänker, K. Lang","doi":"10.1002/SITA.200600088","DOIUrl":"https://doi.org/10.1002/SITA.200600088","url":null,"abstract":"The migration of tumor cells is essential for metastasis development. We distinguish two types of migration with regard to the dynamics and molecular basis: a slow, spontaneous type of migration is induced by the extracellular matrix. This type of migration is myosin II-independent. A fast type of migration is induced by ligands to G protein-coupled receptors, of which norepinephrine is the most potent. This migration depends on the myosin II activity. However, there is still discordance to which part other cytoskeletal components such as actin, myosin and tubulin contribute to these two types of tumor cell migration. Here we present a study on the role of several cytoskeletal proteins in the spontaneous and induced migration of breast and colon carcinoma cells by time-lapse videomicroscopy. The actin reorganization is essential for each type of cell migration. We have shown previously the important function of myosin II for the norepinephrine-induced migration, whereas we show herein that myosin VI is not involved. Most interestingly, stabilization of the microtubules reduced both types of migration, whereas a disruption increased the spontaneous and inhibited the norepinephrine-induced migration. These data suggest that the force generation is different in the spontaneous and the induced migration of carcinoma cells.","PeriodicalId":88702,"journal":{"name":"Signal transduction","volume":"6 2","pages":"270-278"},"PeriodicalIF":0.0,"publicationDate":"2007-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/SITA.200600088","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50950911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Signal transduction mechanisms regulating ion fluxes in the sea urchin sperm 调节海胆精子离子通量的信号转导机制

Signal transduction Pub Date : 2007-04-01 DOI: 10.1002/SITA.200600129

C. Beltrán, B. Galindo, E. Rodríguez‐Miranda, Daniel Sánchez

{"title":"Signal transduction mechanisms regulating ion fluxes in the sea urchin sperm","authors":"C. Beltrán, B. Galindo, E. Rodríguez‐Miranda, Daniel Sánchez","doi":"10.1002/SITA.200600129","DOIUrl":"https://doi.org/10.1002/SITA.200600129","url":null,"abstract":"Ion permeability changes involving ion channels and transporters are essential for fertilization, since they are key elements in sperm-egg signaling and environmental sensing. Changes modulated by external factors and components from the outer layer of the homologous egg, like the sperm-activating peptides and the fucose-sulfate glycoconjugate, trigger complex signaling systems in the sperm that modulate how sperm swim, find the egg and fertilize it. These signaling systems depend on plasma membrane ion permeability and involve an alteration of second messenger levels, changes of membrane potential and intracellular Ca2+, Na+ and pH, along with changes in sperm morphology in the case of the acrosome reaction (AR). New procedures to elucidate the signaling pathways implicated in sperm ion transport have expanded the opportunities to dissect sperm-egg signaling revealing how sperm ion channels participate in activation, motility, chemotaxis, and the AR. The sperm signaling pathways involve a large variety of ion channels and transporters, which are discretely localized and finely orchestrated to play specific roles that define the elaborate performance of sperm and allow a successful fertilization. In this review we will focus on the signaling molecules involved in the final aim of the sperm, to reach and fertilize the egg.","PeriodicalId":88702,"journal":{"name":"Signal transduction","volume":"37 7","pages":"103-117"},"PeriodicalIF":0.0,"publicationDate":"2007-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/SITA.200600129","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50951032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Secondary axis specification in sea urchin embryos 海胆胚胎的次轴发育

Signal transduction Pub Date : 2007-04-01 DOI: 10.1002/SITA.200600121

C. Bradham, D. McClay

{"title":"Secondary axis specification in sea urchin embryos","authors":"C. Bradham, D. McClay","doi":"10.1002/SITA.200600121","DOIUrl":"https://doi.org/10.1002/SITA.200600121","url":null,"abstract":"Secondary axis specification is a process that relies on asymmetric nuclearization of transcription factors in flies and vertebrates, such that the crucial factor is nuclear and therefore functional only in cells along one side of the embryo. In vertebrates, this transcription factor is β-catenin, which is canonically activated downstream from Wnt signals. However, the sea urchin uses asymmetric β-catenin nuclearization during specification of the primary animal-vegetal axis, rather than the secondary oral-aboral (OA) axis. OA specification relies instead on the asymmetric localization of p38 MAPK, a signaling kinase that directly modulates transcription factor activity. A number of genes are expressed in the oral territory downstream from p38, including Nodal and Goosecoid, both of which are associated with secondary axis specification in vertebrate embryos. Because the p38 asymmetry is the earliest known event in the specification of the OA axis, an outstanding question concerns identifying the apparatus upstream from p38 that regulates its asymmetrical activity. Intriguingly, this may be controlled by reactive oxygen species released from the mitochondria, which are asymmetrically distributed about the OA axis.","PeriodicalId":88702,"journal":{"name":"Signal transduction","volume":"22 3","pages":"181-186"},"PeriodicalIF":0.0,"publicationDate":"2007-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/SITA.200600121","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50951238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4