Biochemical Society transactions最新文献_第6页

Insights into mechanisms of ubiquitin ADP-ribosylation reversal. 对泛素 ADP- 核糖逆转机制的见解。

IF 3.8 3区生物学

Biochemical Society transactions Pub Date : 2024-12-19 DOI: 10.1042/BST20240896

Zhengrui Zhang, Chittaranjan Das

{"title":"Insights into mechanisms of ubiquitin ADP-ribosylation reversal.","authors":"Zhengrui Zhang, Chittaranjan Das","doi":"10.1042/BST20240896","DOIUrl":"10.1042/BST20240896","url":null,"abstract":"Ubiquitination and ADP-ribosylation are two types of post-translational modification (PTM) involved in regulating various cellular activities. In a striking example of direct interplay between ubiquitination and ADP-ribosylation, the bacterial pathogen Legionella pneumophila uses its SidE family of secreted effectors to catalyze an NAD+-dependent phosphoribosyl ubiquitination of host substrates in a process involving the intermediary formation of ADP-ribosylated ubiquitin (ADPR-Ub). This noncanonical ubiquitination pathway is finely regulated by multiple Legionella effectors to ensure a balanced host subjugation. Among the various regulatory effectors, the macrodomain effector MavL has been recently shown to reverse the Ub ADP-ribosylation and regenerate intact Ub. Here, we briefly outline emerging knowledge on ubiquitination and ADP-ribosylation and tap into cases of direct cross-talk between these two PTMs. The chemistry of ADP-ribose in the context of the PTM and the reversal mechanisms of ADP-ribosylation are then highlighted. Lastly, focusing on recent structural studies on the MavL-mediated reversal of Ub ADP-ribosylation, we strive to deduce distinct mechanisms regarding the catalysis and product release of this reaction.","PeriodicalId":8841,"journal":{"name":"Biochemical Society transactions","volume":" ","pages":"2525-2537"},"PeriodicalIF":3.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11668277/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142709192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the influence of anionic lipids in the host cell membrane on viral fusion. 探索宿主细胞膜中阴离子脂质对病毒融合的影响。

IF 3.8 3区生物学

Biochemical Society transactions Pub Date : 2024-12-19 DOI: 10.1042/BST20240833

Daniel Birtles, Jinwoo Lee

引用次数: 0

Unusual modes of cell and nuclear divisions characterise Drosophila development. 果蝇的发育具有不寻常的细胞和核分裂模式。

IF 3.8 3区生物学

Biochemical Society transactions Pub Date : 2024-12-19 DOI: 10.1042/BST20231341

Qiaolin Yang, Fernando Wijaya, Ridam Kapoor, Harshaa Chandrasekaran, Siddhant Jagtiani, Izaac Moran, Gary R Hime

{"title":"Unusual modes of cell and nuclear divisions characterise Drosophila development.","authors":"Qiaolin Yang, Fernando Wijaya, Ridam Kapoor, Harshaa Chandrasekaran, Siddhant Jagtiani, Izaac Moran, Gary R Hime","doi":"10.1042/BST20231341","DOIUrl":"10.1042/BST20231341","url":null,"abstract":"The growth and development of metazoan organisms is dependent upon a co-ordinated programme of cellular proliferation and differentiation, from the initial formation of the zygote through to maintenance of mature organs in adult organisms. Early studies of proliferation of ex vivo cultures and unicellular eukaryotes described a cyclic nature of cell division characterised by periods of DNA synthesis (S-phase) and segregation of newly synthesized chromosomes (M-phase) interspersed by seeming inactivity, the gap phases, G1 and G2. We now know that G1 and G2 play critical roles in regulating the cell cycle, including monitoring of favourable environmental conditions to facilitate cell division, and ensuring genomic integrity prior to DNA replication and nuclear division. M-phase is usually followed by the physical separation of nascent daughters, termed cytokinesis. These phases where G1 leads to S phase, followed by G2 prior to M phase and the subsequent cytokinesis to produce two daughters, both identical in genomic composition and cellular morphology are what might be termed an archetypal cell division. Studies of development of many different organs in different species have demonstrated that this stereotypical cell cycle is often subverted to produce specific developmental outcomes, and examples from over 100 years of analysis of the development of Drosophila melanogaster have uncovered many different modes of cell division within this one species.","PeriodicalId":8841,"journal":{"name":"Biochemical Society transactions","volume":" ","pages":"2281-2295"},"PeriodicalIF":3.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11668308/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the dynamics of messenger ribonucleoprotein-mediated translation repression. 探索信使核糖核蛋白介导的翻译抑制动态。

IF 3.8 3区生物学

Biochemical Society transactions Pub Date : 2024-12-19 DOI: 10.1042/BST20231240

Julia Meyer, Marco Payr, Olivier Duss, Janosch Hennig

引用次数: 0

Untangling bacterial DNA topoisomerases functions. 解开细菌 DNA 拓扑异构酶的功能。

IF 3.8 3区生物学

Biochemical Society transactions Pub Date : 2024-12-19 DOI: 10.1042/BST20240089

Céline Borde, Lisa Bruno, Olivier Espéli

引用次数: 0

Advances towards potential cancer therapeutics targeting Hippo signaling. 靶向Hippo信号的潜在癌症治疗进展。

IF 3.8 3区生物学

Biochemical Society transactions Pub Date : 2024-12-19 DOI: 10.1042/BST20240244

Rui Zhu, Zhihan Jiao, Fa-Xing Yu

引用次数: 0

The emerging role of Rab proteins in osteoclast organelle biogenesis and function. Rab蛋白在破骨细胞细胞器生物发生和功能中的新作用。

IF 3.8 3区生物学

Biochemical Society transactions Pub Date : 2024-12-19 DOI: 10.1042/BST20240519

Shiou-Ling Lu, Takeshi Noda

引用次数: 0

Quaternary arrangements of membrane proteins: an aquaporin case. 膜蛋白的四级排列：水蒸发蛋白案例。

IF 3.8 3区生物学

Biochemical Society transactions Pub Date : 2024-12-19 DOI: 10.1042/BST20241630

Maria Hrmova

{"title":"Quaternary arrangements of membrane proteins: an aquaporin case.","authors":"Maria Hrmova","doi":"10.1042/BST20241630","DOIUrl":"10.1042/BST20241630","url":null,"abstract":"Integral polytopic α-helical membrane transporters and aquaporins move and distribute various molecules and dispose of or compartmentalize harmful elements that gather in living cells. The view shaped nearly 25 years ago states that integrating these proteins into cellular membranes can be considered a two-stage process, with hydrophobic core folding into α-helices across membranes to form functional entities (Popot and Engelman, 1990; Biochemistry29, 4031-4037). Since then, a large body of evidence cemented the roles of structural properties of membrane proteins and bilayer solvent components in forming functional assemblies. This mini-review updates our understanding of multifaced factors, which underlie transporters integration and oligomerization, focusing on water-permeating aquaporins. This work also elaborates on how individual monomers of bacterial and mammalian aquaporin tetramers, interact with each other, and how tetramers form contacts with lipids after being embedded in lipid bilayers of known composition, which mimics bacterial and mammalian membranes. Although this mini-review describes findings acquired using current methods, the view is open to how to extend this knowledge through, e.g. single-molecule-based and in situ cryogenic-electron tomography techniques. These and other methods could unravel the sources of entropy for membrane protein assemblies and pathways underlying integration, folding, oligomerization and quaternary structure formation with binding partners. We could expect that these exceedingly interdisciplinary approaches will form the basis for creating optimized transport systems, which could inspire bioengineering to develop a sustainable and healthy society.","PeriodicalId":8841,"journal":{"name":"Biochemical Society transactions","volume":" ","pages":"2557-2568"},"PeriodicalIF":3.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11668299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulatory dynamics of arginine metabolism in Staphylococcus aureus. 金黄色葡萄球菌精氨酸代谢的调控动力学。

IF 3.8 3区生物学

Biochemical Society transactions Pub Date : 2024-12-19 DOI: 10.1042/BST20240710

Itidal Reslane, Gabrielle F Watson, Luke D Handke, Paul D Fey

引用次数: 0

Advances in utilizing reverse micelles to investigate membrane proteins. 利用反向胶束研究膜蛋白的进展。

IF 3.8 3区生物学

Biochemical Society transactions Pub Date : 2024-12-19 DOI: 10.1042/BST20240830

Sara H Walters, Aaron S Birchfield, Brian Fuglestad

{"title":"Advances in utilizing reverse micelles to investigate membrane proteins.","authors":"Sara H Walters, Aaron S Birchfield, Brian Fuglestad","doi":"10.1042/BST20240830","DOIUrl":"10.1042/BST20240830","url":null,"abstract":"Reverse micelles (RMs) have emerged as useful tools for the study of membrane associated proteins. With a nanoscale water core surrounded by surfactant and solubilized in a non-polar solvent, RMs stand apart as a unique membrane model. While RMs have been utilized as tools to investigate the physical properties of membranes and their associated water, RMs also effectively house membrane associated proteins for a variety of studies. High-resolution protein NMR revealed a need for development of improved RM formulations, which greatly enhanced the use of RMs for aqueous proteins. Protein-optimized RM formulations enabled encapsulation of challenging membrane associated protein types, including lipidated proteins, transmembrane proteins, and peripheral membrane proteins. Improvements in biological accuracy of RMs using phospholipid-based surfactants has advanced their utility as a membrane mimetic even further, better matching the chemistry of the most common cellular membrane lipids. Natural lipid extracts may also be used to construct RMs and house proteins, resulting in a membrane model that better represents the complexity of biological membranes. Recent applications in high-resolution investigations of protein-membrane interactions and inhibitor design of membrane associated proteins have demonstrated the usefulness of these systems in addressing this difficult category of protein. Further developments of RMs as membrane models will enhance the breadth of investigations facilitated by these systems and will enhance their use in biophysical, structural, and drug discovery pursuits of membrane associated proteins. In this review, we present the development of RMs as membrane models and their application to structural and biophysical study of membrane proteins.","PeriodicalId":8841,"journal":{"name":"Biochemical Society transactions","volume":" ","pages":"2499-2511"},"PeriodicalIF":3.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11659023/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0