Biochemical and molecular medicine最新文献_第6页

An Enzyme Immunoassay for Neuron-Specific Enolase in Cerebrospinal Fluid 脑脊液中神经元特异性烯醇化酶的酶免疫测定

Biochemical and molecular medicine Pub Date : 1997-06-01 DOI: 10.1006/bmme.1997.2595

Elmo N. Orlino Jr. , Charles E. Olmstead , Jorge A. Lazareff , Warwick J. Peacock , Robin S. Fisher , Arvan L. Fluharty

{"title":"An Enzyme Immunoassay for Neuron-Specific Enolase in Cerebrospinal Fluid","authors":"Elmo N. Orlino Jr. , Charles E. Olmstead , Jorge A. Lazareff , Warwick J. Peacock , Robin S. Fisher , Arvan L. Fluharty","doi":"10.1006/bmme.1997.2595","DOIUrl":"10.1006/bmme.1997.2595","url":null,"abstract":"<div>A direct (as opposed to competitive) enzyme immunoassay (EIA) was developed to detect neuron-specific enolase (NSE) in cerebrospinal fluid (CSF). Most common methods of evaluating NSE levels have utilized radioimmunoassay. These are highly sensitive, but cannot be employed in laboratories not equipped or licensed for the use of radioisotopes. The EIA developed here shows sensitivity within the physiological range of values for CSF-NSE (>1 ng/ml) and can be used in laboratories with appropriate densitometric scanning capabilities. The assay was applied to CSF samples obtained from patients with a variety of diagnoses at the time of surgical intervention for their respective disorders. While there were no diagnostically significant differences between the levels of NSE in CSF from patients with different neurological disorders utilized in the development of this procedure, we were able to differentiate between marginally different levels of NSE. We conclude that we have developed a safe, fast, reliable, and sensitive assay for NSE in the CSF that can be used to study NSE levels in a variety of neurological cases.</div>","PeriodicalId":8837,"journal":{"name":"Biochemical and molecular medicine","volume":"61 1","pages":"Pages 41-46"},"PeriodicalIF":0.0,"publicationDate":"1997-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/bmme.1997.2595","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20176420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

The Mechanisms of Compound 48/80-Induced Superoxide Generation Mediated by A-Kinase in Rat Peritoneal Mast Cells a激酶介导的化合物48/80诱导大鼠腹膜肥大细胞产生超氧化物的机制

Biochemical and molecular medicine Pub Date : 1997-06-01 DOI: 10.1006/bmme.1997.2594

Nobuyuki Fukuishi , Masakiyo Sakaguchi , Shiori Matsuura , Chizuko Nakagawa , Reiko Akagi , Masaaki Akagi

{"title":"The Mechanisms of Compound 48/80-Induced Superoxide Generation Mediated by A-Kinase in Rat Peritoneal Mast Cells","authors":"Nobuyuki Fukuishi , Masakiyo Sakaguchi , Shiori Matsuura , Chizuko Nakagawa , Reiko Akagi , Masaaki Akagi","doi":"10.1006/bmme.1997.2594","DOIUrl":"10.1006/bmme.1997.2594","url":null,"abstract":"<div>This investigation was undertaken to clarify the mechanisms of superoxide anion (O2−) generation in rat peritoneal mast cells. Compound 48/80, a typical histamine liberator mediated by calcium influx, elicited O2−generation from the mast cells in a dose-dependent fashion. It was demonstrated by immunohistochemical study and Western blot analysis that the mast cells contained the 47-kDa phagocyte oxidase (p47phox) protein, which was one cytosolic component of the NADPH oxidase system. Arachidonic acid stimulated O2−generation in the mast cells, but other unsaturated fatty acids had no effect. On the other hand, 48/80-induced O2−generation was inhibited by phospholipase A2 inhibitors, such as arachidonyl trifluoromethyl ketone and manoalide. Forskolin, isoprenaline, and dibutyryl cyclic AMP inhibited the O2−generation, and KT-5720, a cyclic AMP-dependent protein kinase (A-kinase) inhibitor, markedly enhanced the O2−generation. These findings suggest that O2−is generated by a NADPH oxidase-like enzyme system in mast cells and that this enzyme system is activated by arachidonic acid released by cytosolic phospholipase A2. Thus, it is regulated by the cyclic AMP-A kinase system.</div>","PeriodicalId":8837,"journal":{"name":"Biochemical and molecular medicine","volume":"61 1","pages":"Pages 107-113"},"PeriodicalIF":0.0,"publicationDate":"1997-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/bmme.1997.2594","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20177433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34

Effects of Dehydroepiandrosterone in Rats Injected with Streptozotocin during the Neonatal Period 脱氢表雄酮对新生儿期注射链脲佐菌素大鼠的影响

Biochemical and molecular medicine Pub Date : 1997-06-01 DOI: 10.1006/bmme.1997.2581

M.-H. Giroix , F. Malaisse-Lagae , B. Portha , A. Sener , W.J. Malaisse

引用次数: 12

Human Seminal Plasma Soluble 5′-Nucleotidase: Regulatory Aspects of the Dephosphorylation of Nucleoside 5′-Monophosphates 人精浆可溶性5′-核苷酸酶:核苷5′-单磷酸去磷酸化的调控方面

Biochemical and molecular medicine Pub Date : 1997-06-01 DOI: 10.1006/bmme.1997.2589

Alba Minelli, Monica Moroni, Isabella Mezzasoma

{"title":"Human Seminal Plasma Soluble 5′-Nucleotidase: Regulatory Aspects of the Dephosphorylation of Nucleoside 5′-Monophosphates","authors":"Alba Minelli, Monica Moroni, Isabella Mezzasoma","doi":"10.1006/bmme.1997.2589","DOIUrl":"10.1006/bmme.1997.2589","url":null,"abstract":"<div>Human seminal plasma contains two enzyme activities both capable of dephosphorylating all nucleoside 5-monophosphates with different efficiency and specificity. Broad-spectrum soluble 5′-nucleotidase is the object of this paper which deals with the definition of the response of this enzyme to effectors, some physiological and others not naturally occurring. The enzyme did not show any product regulation as all the nucleosides tested caused a moderate effect on the hydrolysis of the substrates. Theophylline and other xanthine derivatives had no effect on enzyme activity, whereas glycerate 2,3bisphosphate, like other soluble 5′-nucleotidases, caused a stimulation of the enzyme, especially toward CMP and UMP. 5-Deoxy-5-isobutylthiadenosine resulted in no inhibition of the hydrolysis of AMP and IMP. The enzyme was affected neither by monovanadate nor by decavanadate, whereas it was strongly inhibited by Ap5A. Variations in adenylate energy charge did not cause any alteration of the enzyme activity toward AMP and only a slight decrease of the hydrolysis of IMP. These regulatory properties, distinct from those of other soluble 5′-nucleotidases, show that this form, newly isolated from human seminal plasma, is subject to an almost unique, tissue-specific regulation.</div>","PeriodicalId":8837,"journal":{"name":"Biochemical and molecular medicine","volume":"61 1","pages":"Pages 95-101"},"PeriodicalIF":0.0,"publicationDate":"1997-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/bmme.1997.2589","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20176315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Histidine Modification of Human Serum Butyrylcholinesterase 人血清丁基胆碱酯酶的组氨酸修饰

Biochemical and molecular medicine Pub Date : 1997-06-01 DOI: 10.1006/bmme.1997.2578

D. Cengiz, A.N. Çokugraş, K. Kilinç, E.F. Tezcan

{"title":"Histidine Modification of Human Serum Butyrylcholinesterase","authors":"D. Cengiz, A.N. Çokugraş, K. Kilinç, E.F. Tezcan","doi":"10.1006/bmme.1997.2578","DOIUrl":"10.1006/bmme.1997.2578","url":null,"abstract":"<div>The effects of histidine-modifying reagents on human serum butyrylcholinesterase (BChE) were investigated. The commercially available enzyme was further purified by chromatography on a Sepharose CI-6B column prior to use. In the modification studies, we found that the histidine-specific reagents tosylphenylalanine chloromethyl ketone (TPCK) and tosyllysine chloromethyl ketone (TLCK) did not modify the enzyme; however, they inhibited the enzyme reversibly. The kinetic parameters of enzyme inhibition calculated were α = 10.8, β = 0.26, andKi= 0.016 mmfor TPCK. TLCK inhibition gave similar kinetic behavior, with α = 41.6, β = 0.065, andKi= 0.039 mm. Tosyllysine, an analog of TLCK, did not inhibit the enzyme. Removal of TPCK and TLCK by dialysis resulted in significant reactivation of the enzyme. From kinetic studies, it was found that the inhibitions were hyperbolic mixed-type inhibitions. We concluded that the reagents competed with substrate for hydrophobic binding sites and inhibited the enzyme reversibly. On the other hand, in the modification studies with diethyl pyrocarbonate (DPC), it was observed that inactivation of the enzyme was irreversible and time-dependent. In the protection studies, the activity of the enzyme was partially protected from inactivation by DPC even at a 50 mmconcentration of butyrylthiocholine. The results indicate that DPC modifies some essential histidine side chains in BChE, including the functional histidyl residue found at the active site.</div>","PeriodicalId":8837,"journal":{"name":"Biochemical and molecular medicine","volume":"61 1","pages":"Pages 52-57"},"PeriodicalIF":0.0,"publicationDate":"1997-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/bmme.1997.2578","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20176309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Comparison of the Hydrolysis of the Three Types of Natriuretic Peptides by Human Kidney Neutral Endopeptidase 24.11 人肾中性内肽酶水解三种利钠肽的比较24.11

Biochemical and molecular medicine Pub Date : 1997-06-01 DOI: 10.1006/bmme.1997.2584

Yasuhiro Watanabe, Kenjiro Nakajima, Yoshimitsu Shimamori, Yukio Fujimoto

引用次数: 58

Leukotriene Synthesis by Isolated Perinatal Ovine Intrapulmonary Vessels Correlates with Age-Related Changes in 5-Lipoxygenase Protein 围产儿绵羊肺内血管白三烯合成与5-脂氧合酶蛋白的年龄相关变化

Biochemical and molecular medicine Pub Date : 1997-06-01 DOI: 10.1006/bmme.1997.2579

Basil O. Ibe, Jean M. Anderson, J.Usha Raj

{"title":"Leukotriene Synthesis by Isolated Perinatal Ovine Intrapulmonary Vessels Correlates with Age-Related Changes in 5-Lipoxygenase Protein","authors":"Basil O. Ibe, Jean M. Anderson, J.Usha Raj","doi":"10.1006/bmme.1997.2579","DOIUrl":"10.1006/bmme.1997.2579","url":null,"abstract":"<div>Leukotrienes (LT) are potent vasoconstrictors in the pulmonary circulation. We have investigated the synthesis of LTC4by intrapulmonary arteries and veins of perinatal lambs. Paired vessels of near-term fetal 146 ± 2- and 2- to 7-day-old newborn lambs (eachn= 7), were incubated for 10 min at 37°C in baseline, with 1 μmol/L A32187 or 0.1 mmol/L arachidonic acid. Produced leukotriene C4was assayed from media by ELISA. Baseline production of leukotriene (ng/mg tissue, means ± SEM) by vessels for arteries was 0.006 ± 0.001 and 0.059 ± 0.009 for fetus and newborn, respectively. In veins, the values were 0.013 ± 0.003 and 0.073 ± 0.007 for fetus and newborn, respectively. On stimulation with the calcium ionophore A23187, production by arteries increased 25-fold in the fetus, but 4-fold in the newborn. The corresponding values for stimulated veins were 37-fold and 9-fold in fetus and newborn, respectively. Generally, production by veins was greater than production by the matching arteries. In all instances, the fetal vessels produced less leukotrienes than the newborn vessels. Western analysis of stimulated and unstimulated vessel membrane protein showed greater expression of 5-lipoxygenase in veins than in arteries (P< 0.05). Our data show that veins produce more LTC4due to greater expression of 5-lipoxygenase in the vessels and thus suggest that veins of perinatal lamb lungs may be more susceptible to LT-induced vasoreactivity in the perinatal pulmonary circulation. We speculate that a higher production of LTC4by fetal veins may be necessary to maintain a high venous tone in fetal lungs.</div>","PeriodicalId":8837,"journal":{"name":"Biochemical and molecular medicine","volume":"61 1","pages":"Pages 63-71"},"PeriodicalIF":0.0,"publicationDate":"1997-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/bmme.1997.2579","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20176311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

DAX1 Gene Expression Upregulated by Steroidogenic Factor 1 in an Adrenocortical Carcinoma Cell Line 类固醇生成因子1上调DAX1基因在肾上腺皮质癌细胞中的表达

Biochemical and molecular medicine Pub Date : 1997-06-01 DOI: 10.1006/bmme.1997.2601

Eric Vilain, Weiwen Guo, Yao-Hua Zhang, Edward R.B. McCabe

{"title":"DAX1 Gene Expression Upregulated by Steroidogenic Factor 1 in an Adrenocortical Carcinoma Cell Line","authors":"Eric Vilain, Weiwen Guo, Yao-Hua Zhang, Edward R.B. McCabe","doi":"10.1006/bmme.1997.2601","DOIUrl":"10.1006/bmme.1997.2601","url":null,"abstract":"<div>Two nuclear hormone receptor superfamily members, DAX1 and SF1, are required for normal adrenal cortical development. Mutations in DAX1 are responsible for X-linked adrenal hypoplasia congenita (AHC) and hypogonadotropic hypogonadism. Steroidogenic Factor 1 (SF1) regulates the expression of a number of steroidogenic genes and a putative SF1 response element (SF1-RE) in the DAX1 promoter which binds SF1 specifically. Therefore, we examined deletions in the DAX1 promoter driving expression of β-galactosidase, with and without coexpression of SF1, in the human adrenocortical carcinoma cell line NCI-H295. We defined the DAX initiation start site and localized the putative SF1-RE at −135 to −143 bp. Loss of the putative SF1-RE region or specific removal of the 9-bp SF1 site resulted in decreased transcriptional activity by 2.3- to 2.5-fold. When cotransfected with 1550 bp of the DAX1 promoter, an SF1-containing expression vector increased the transcriptional activity of the DAX1 promoter by 4-fold. No significant change above baseline occurred when the cells were cotransfected with the 1541-bp fragment containing the entire 1550-bp promoter region minus the 9-bp SF1-RE. We conclude that the SF1-RE is an enhancer element within the DAX1 promoter and speculate that SF1 may be a transcription factor that acts, at least in part, through DAX1 for normal adrenal cortical development.</div>","PeriodicalId":8837,"journal":{"name":"Biochemical and molecular medicine","volume":"61 1","pages":"Pages 1-8"},"PeriodicalIF":0.0,"publicationDate":"1997-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/bmme.1997.2601","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20176415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 56

Expression Study of Survival Motor Neuron Gene in Human Fetal Tissues 存活运动神经元基因在人胎儿组织中的表达研究

Biochemical and molecular medicine Pub Date : 1997-06-01 DOI: 10.1006/bmme.1997.2590

G. Novelli , L. Calzà , P. Amicucci , L. Giardino , M. Pozza , V. Silani , A. Pizzuti , M. Gennarelli , G. Piombo , F. Capon , B. Dallapiccola

引用次数: 30

Identification of a Splice Site Mutation in the Cystathionine β-Synthase Gene Resulting in Variable and Novel Splicing Defects of Pre-mRNA 半胱硫氨酸β合酶基因剪接位点突变的鉴定导致Pre-mRNA的可变和新的剪接缺陷

Biochemical and molecular medicine Pub Date : 1997-06-01 DOI: 10.1006/bmme.1997.2591

Michael Y. Tsai , Paul W.K. Wong , Uttam Garg , Naomi Q. Hanson , Kerry Schwichtenberg

引用次数: 13