人精浆可溶性5′-核苷酸酶:核苷5′-单磷酸去磷酸化的调控方面

Alba Minelli, Monica Moroni, Isabella Mezzasoma
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引用次数: 7

摘要

人精浆含有两种酶活性,它们都能以不同的效率和特异性去磷酸化所有核苷5-单磷酸。广谱可溶性5′-核苷酸酶是本文的研究对象,讨论了该酶对一些生理效应物和其他非自然产生的效应物的反应的定义。该酶没有表现出任何产物调节,因为所有测试的核苷对底物的水解都有适度的影响。茶碱和其他黄嘌呤衍生物对酶活性没有影响,而甘油2,3二磷酸和其他可溶性5 ' -核苷酸酶一样,对酶活性有刺激作用,特别是对CMP和UMP。5-脱氧-5-异丁基硫腺苷对AMP和IMP的水解没有抑制作用,单钒酸盐和十钒酸盐对酶的水解没有影响,而Ap5A对酶的水解有强烈的抑制作用。腺苷酸能量电荷的变化不会引起酶对AMP的活性的任何改变,只是对IMP的水解有轻微的降低。这些不同于其他可溶性5 ' -核苷酸酶的调节特性表明,这种新从人类精浆中分离出来的形式受到一种几乎独特的组织特异性调节。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Human Seminal Plasma Soluble 5′-Nucleotidase: Regulatory Aspects of the Dephosphorylation of Nucleoside 5′-Monophosphates

Human seminal plasma contains two enzyme activities both capable of dephosphorylating all nucleoside 5-monophosphates with different efficiency and specificity. Broad-spectrum soluble 5′-nucleotidase is the object of this paper which deals with the definition of the response of this enzyme to effectors, some physiological and others not naturally occurring. The enzyme did not show any product regulation as all the nucleosides tested caused a moderate effect on the hydrolysis of the substrates. Theophylline and other xanthine derivatives had no effect on enzyme activity, whereas glycerate 2,3bisphosphate, like other soluble 5′-nucleotidases, caused a stimulation of the enzyme, especially toward CMP and UMP. 5-Deoxy-5-isobutylthiadenosine resulted in no inhibition of the hydrolysis of AMP and IMP. The enzyme was affected neither by monovanadate nor by decavanadate, whereas it was strongly inhibited by Ap5A. Variations in adenylate energy charge did not cause any alteration of the enzyme activity toward AMP and only a slight decrease of the hydrolysis of IMP. These regulatory properties, distinct from those of other soluble 5′-nucleotidases, show that this form, newly isolated from human seminal plasma, is subject to an almost unique, tissue-specific regulation.

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