Biochemistry and Molecular Biology Education最新文献_第9页

Feasible and economic DIY procedures of a hand-held fluorescence detector set—FluorDetector to assist laboratory course development 手持式荧光探测器--FluorDetector 的可行和经济 DIY 程序，以协助实验室课程开发。

IF 0.9 4区教育学

Biochemistry and Molecular Biology Education Pub Date : 2024-06-25 DOI: 10.1002/bmb.21846

Bo Ding, Ju Wang, Yaxin Mao, Tian Meng, Yumei Luo, Xiaomei Xu, Zhanqing Lv, Mian Zhou, Qiuying Yang

{"title":"Feasible and economic DIY procedures of a hand-held fluorescence detector set—FluorDetector to assist laboratory course development","authors":"Bo Ding, Ju Wang, Yaxin Mao, Tian Meng, Yumei Luo, Xiaomei Xu, Zhanqing Lv, Mian Zhou, Qiuying Yang","doi":"10.1002/bmb.21846","DOIUrl":"10.1002/bmb.21846","url":null,"abstract":"Fluorescence-related experimental techniques play an important role in biochemistry, molecular biology, and cell biology. However, fluorescence-related experiments are rarely included in the laboratory courses of most Chinese universities. This is mainly due to the conflict between large class size (50–60 students in one room) and funding/space limitations to purchase and accommodate enough fluorescence detection equipment. Here, we proposed feasible and economical Do It Yourself (DIY) procedures of a hand-held fluorescence detector set—FluorDetector to support the development of laboratory courses. Tested on several samples, clear fluorescence signals could be directly observed by FluorDetector and photographed with a smartphone. In addition, FluorDetector was able to turn a conventional stereomicroscope into a fluorescence stereomicroscope, detecting fluorescence signals with clean background. FluorDetector is easy to make with a 3D printer, with an extremely low cost ($200 each) when compared with a commercial fluorescence microscope or fluorescence stereomicroscope, and almost as sensitive as a microplate reader in measuring fluorescence. Therefore, FluorDetector is a possible strategy to solve the problem and help to integrate fluorescence-related experimental modules in laboratory courses.","PeriodicalId":8830,"journal":{"name":"Biochemistry and Molecular Biology Education","volume":"52 5","pages":"580-587"},"PeriodicalIF":0.9,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141454904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"教育学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A cost-effective enzyme kinetics and inhibition model for biochemistry education and research 用于生物化学教育和研究的经济高效的酶动力学和抑制模型。

IF 0.9 4区教育学

Biochemistry and Molecular Biology Education Pub Date : 2024-06-15 DOI: 10.1002/bmb.21845

Lauren VanDee, Alyssa Teague, Taylor East, Kim Rhona Jacinto, Macie Carter, Jacey Totty, Paul D. Adams, Djamali Muhoza

{"title":"A cost-effective enzyme kinetics and inhibition model for biochemistry education and research","authors":"Lauren VanDee, Alyssa Teague, Taylor East, Kim Rhona Jacinto, Macie Carter, Jacey Totty, Paul D. Adams, Djamali Muhoza","doi":"10.1002/bmb.21845","DOIUrl":"10.1002/bmb.21845","url":null,"abstract":"Enzyme kinetics and inhibition studies are crucial in biochemistry education and research. Conventional methods often require expensive equipment and reagents, potentially limiting their accessibility in limited resource settings. Our approach sought to develop a cost-effective experimental design for studying enzyme kinetics and inhibition. Lactase was chosen as a protein model and its activity was investigated by measuring glucose production from lactose hydrolysis. In the study, commercially available lactase pills were used as an enzyme source, while milk was used as a substrate. Instead of scientific equipment, glucometers were used to measure lactase activity. Enzyme kinetics were evaluated using Michaelis–Menten and Lineweaver–Burk plots. In the study, the effects of temperature, pH, and inhibitors were also investigated. The results of our study aligned with established enzyme kinetics theories and previous studies. Lactase showed temperature and pH-dependent activity, with decreased activity observed at both low and high extremes. Results also showed that galactose acts as a competitive inhibitor of lactase. The approach presented here offers a cost-effective procedure for studying enzyme kinetics and inhibition. It can act as a valuable tool for educational purposes and for preliminary research in settings with limited resources.","PeriodicalId":8830,"journal":{"name":"Biochemistry and Molecular Biology Education","volume":"52 5","pages":"588-598"},"PeriodicalIF":0.9,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bmb.21845","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141327197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"教育学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular and genetics inquiry program using Drosophila eyes absent gene 使用果蝇眼球缺失基因的分子和遗传学探究程序。

IF 0.9 4区教育学

Biochemistry and Molecular Biology Education Pub Date : 2024-06-14 DOI: 10.1002/bmb.21844

Jinhyeon Choi, Sang-Hak Jeon, Hyeon-Pyo Shim

引用次数: 0

Constructing analogies: Developing critical thinking through a collaborative task 构建类比：通过协作任务发展批判性思维

IF 0.9 4区教育学

Biochemistry and Molecular Biology Education Pub Date : 2024-06-08 DOI: 10.1002/bmb.21843

Constantine Kapetanakis, Samantha Conflitti, Sarah Abdo, L. Kate Wright, Dina L. Newman

{"title":"Constructing analogies: Developing critical thinking through a collaborative task","authors":"Constantine Kapetanakis, Samantha Conflitti, Sarah Abdo, L. Kate Wright, Dina L. Newman","doi":"10.1002/bmb.21843","DOIUrl":"10.1002/bmb.21843","url":null,"abstract":"Analogies are used to make abstract topics meaningful and more easily comprehensible to learners. Incorporating simple analogies into STEM classrooms is a fairly common practice, but the analogies are typically generated and explained by the instructor for the learners. We hypothesize that challenging learners to create complex, extended analogies themselves can promote integration of content knowledge and development of critical thinking skills, which are essential for deep learning, but are challenging to teach. In this qualitative study, college biology students (n = 30) were asked to construct a complex analogy about the flow of genetic information using a familiar item. One week later, participants constructed a second analogy about the same topic, but this time using a more challenging item. Twenty participants worked on the challenging analogy in pairs, while the other 10 worked alone. Analysis of the 50 interviews resulted in a novel-scoring scheme, which measured both content knowledge (understanding of biology terms) and critical thinking (alignment of relationships between elements of the analogy). Most participants improved slightly due to practice, but they improved dramatically when working with a partner. The biggest gains were seen in critical thinking, not content knowledge. Having students construct complex, sophisticated analogies in pairs is a high-impact practice that can help students develop their critical thinking skills, which are crucial in academic and professional settings. The discussion between partners likely requires students to justify their explanations and critique their partner's explanations, which are characteristics of critical thinking.","PeriodicalId":8830,"journal":{"name":"Biochemistry and Molecular Biology Education","volume":"52 5","pages":"569-579"},"PeriodicalIF":0.9,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141293057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"教育学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green, yellow, or cyan? Introduction of color change mutations into a green thermostable fluorescent protein and characterization during an introduction to biochemistry lab course 绿色、黄色还是青色？在生物化学入门实验课程中引入绿色恒温荧光蛋白的变色突变并进行表征。

IF 0.9 4区教育学

Biochemistry and Molecular Biology Education Pub Date : 2024-06-08 DOI: 10.1002/bmb.21841

Matthew R. Anderson, Cammi J. Dargatz, Tuhina Banerjee, Natasha M. DeVore

{"title":"Green, yellow, or cyan? Introduction of color change mutations into a green thermostable fluorescent protein and characterization during an introduction to biochemistry lab course","authors":"Matthew R. Anderson, Cammi J. Dargatz, Tuhina Banerjee, Natasha M. DeVore","doi":"10.1002/bmb.21841","DOIUrl":"10.1002/bmb.21841","url":null,"abstract":"Green fluorescent protein has long been a favorite protein for demonstrating protein purification in the biochemistry lab course. The protein's vivid green color helps demonstrate to students the concept(s) behind affinity or ion exchange chromatography. We designed a series of introduction to biochemistry labs utilizing a thermostable green protein (TGP-E) engineered to have unusually high thermostability. This protein allows students to proceed through purification and characterization without the need to keep protein samples on ice. The 5-week lab series begins with an introduction to molecular biology techniques during weeks 1 and 2, where site-directed mutagenesis is used introduce, a single nucleotide change that shifts the fluorescent spectra of TGP-E to either cyan (CTP-E) or yellow (YTP-E). Students identify successful mutagenesis reaction by the color of a small expression sample after induction with IPTG. Next, students purify either the TGP-E (control—typically one group volunteers), YTP-E, or CTP-E protein as a 1-week lab. During the following week's lab, students run SDS-PAGE to verify protein purity, bicinchoninic acid assay to quantify protein yield, and absorbance and fluorescence spectra to characterize their protein's fluorescent character. The final lab in the series investigates the thermostability of YTP-E and CTP-E compared with TGP-E using a fluorescence plate reader. This 5-week series of experiments provide students with experience in several key biochemistry techniques and allows the students to compare properties of mutations. At the end of the course, the students will write a research report and give a short presentation over their results.","PeriodicalId":8830,"journal":{"name":"Biochemistry and Molecular Biology Education","volume":"52 5","pages":"549-558"},"PeriodicalIF":0.9,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141293058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"教育学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing teaching effectiveness in biochemistry labs: Author reflections and improvement strategies 提高生物化学实验室的教学效果：作者反思与改进策略。

IF 0.9 4区教育学

Biochemistry and Molecular Biology Education Pub Date : 2024-05-30 DOI: 10.1002/bmb.21842

Maurizio Costabile, Bradley Simpson, Jasmina Turkanovic, Bernard P. Hughes

{"title":"Enhancing teaching effectiveness in biochemistry labs: Author reflections and improvement strategies","authors":"Maurizio Costabile, Bradley Simpson, Jasmina Turkanovic, Bernard P. Hughes","doi":"10.1002/bmb.21842","DOIUrl":"10.1002/bmb.21842","url":null,"abstract":"This article details the outcome of a joint reflective approach undertaken by the authors to identify common difficulties experienced by 2nd-year undergraduate Biochemistry students in laboratory classes. Difficulties experienced in laboratories can affect the development of hand skills, an understanding of how to correctly operate laboratory equipment and the linkage between didactic content and their experimental demonstration. These difficulties covered were identified based on their common appearance across multiple cohorts and are grouped into five broad areas. The context of the laboratory exercises is detailed and the common difficulties experienced by students are outlined. The potential causes of these difficulties are then discussed along with the approaches and strategies that were implemented to help resolve future occurrences. The approach and resources developed to address these difficulties may help other Biochemistry educators who are facing similar experiences with their undergraduate students.","PeriodicalId":8830,"journal":{"name":"Biochemistry and Molecular Biology Education","volume":"52 5","pages":"559-568"},"PeriodicalIF":0.9,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bmb.21842","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141174036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"教育学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-impact practices in cancer education and research: Undergraduate students' perceptions of skills and career development 癌症教育与研究中的高效实践：本科生对技能和职业发展的看法。

IF 0.9 4区教育学

Biochemistry and Molecular Biology Education Pub Date : 2024-05-30 DOI: 10.1002/bmb.21839

Mohammad A. Al Hakani, Edward A. Grimmett, Eleftheria Laios, Jake Frank, Subidsa Srikantha, Kaila Wilson, Karen Metcalfe, Caroline Hamm, Lisa A. Porter, Dora Cavallo-Medved

{"title":"High-impact practices in cancer education and research: Undergraduate students' perceptions of skills and career development","authors":"Mohammad A. Al Hakani, Edward A. Grimmett, Eleftheria Laios, Jake Frank, Subidsa Srikantha, Kaila Wilson, Karen Metcalfe, Caroline Hamm, Lisa A. Porter, Dora Cavallo-Medved","doi":"10.1002/bmb.21839","DOIUrl":"10.1002/bmb.21839","url":null,"abstract":"High-impact practices (HIPs) are educational practices that foster student success. HIPs have not been widely used in cancer education and research despite the need for students to develop key transferable skills and cultivate social responsibility. Our study addresses this need by implementing four community-based learning HIPs within the context of cancer education and research. Each HIP was classified as having low, moderate, or high alignment with the traits of effective HIPs. Undergraduate science students participated in one to four HIPs as a Feedback Participant, General Volunteer, Student Leader, or Cancer Undergraduate Research and Education (CURES) Class Student. We then studied the effect of these HIPs on students' development of knowledge and skills; career interest and preparedness; and social responsibility. Results from self-reported questionnaires showed that HIPs increased students' cancer knowledge and developed their transferable and technical skills. Many students reported that these HIPs strongly impacted their career preparedness; positively influenced their interest in pursuing careers in health or biomedical sciences; and encouraged them to participate in community service activities. Thus, these findings provide new insights into the perceived benefits of HIPs in cancer education and research by undergraduate students.","PeriodicalId":8830,"journal":{"name":"Biochemistry and Molecular Biology Education","volume":"52 5","pages":"527-539"},"PeriodicalIF":0.9,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bmb.21839","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141174093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"教育学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving critical thinking skills of preservice chemistry teachers through integrated biochemistry course 通过综合生物化学课程提高职前化学教师的批判性思维能力。

IF 0.9 4区教育学

Biochemistry and Molecular Biology Education Pub Date : 2024-05-28 DOI: 10.1002/bmb.21840

Andi Wahyudi, Yusinta Dwi Ariyani

{"title":"Improving critical thinking skills of preservice chemistry teachers through integrated biochemistry course","authors":"Andi Wahyudi, Yusinta Dwi Ariyani","doi":"10.1002/bmb.21840","DOIUrl":"10.1002/bmb.21840","url":null,"abstract":"Many studies have reported various interventions to increase critical thinking, but very few studies have examined the impact of learning in classrooms and laboratories. This study aimed to find a learning pattern (practice to theory or theory to practice) in improving students' critical thinking skills (CTs). Pre and posttest nonequivalent group design was employed in this study. Eighty preservice chemistry teachers divided 40 students in experimental class 1 and 40 in experimental class 2. A test of enzyme-CTs was developed to measure student CTs before and after the intervention. The result showed that integrated biochemistry courses could improve students' CTs. An independent sample t-test was employed, and the result showed a significant difference N-gain students' CTs between experimental classes 1 and 2 (p = 0.018). It indicates that the pattern of developing CTs from practice to theory is better than theory to practice. The research result can be taken into consideration for placing biochemistry theory and biochemistry practicum in the same semester for the chemistry or chemistry education curriculum. Students can find concepts independently in practical activities and develop them in theoretical activities. Further research should analyze the discriminant factors that differentiate between students in experimental classes 1 and 2.","PeriodicalId":8830,"journal":{"name":"Biochemistry and Molecular Biology Education","volume":"52 5","pages":"540-548"},"PeriodicalIF":0.9,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141157281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"教育学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing complex bioprocess learning through simulation technology and hybrid teaching: A case study in university education 通过模拟技术和混合教学加强复杂生物工艺学习：大学教育案例研究。

IF 0.9 4区教育学

Biochemistry and Molecular Biology Education Pub Date : 2024-05-06 DOI: 10.1002/bmb.21838

Davor Cotoras

{"title":"Enhancing complex bioprocess learning through simulation technology and hybrid teaching: A case study in university education","authors":"Davor Cotoras","doi":"10.1002/bmb.21838","DOIUrl":"10.1002/bmb.21838","url":null,"abstract":"The utilization of computer simulators in university education is progressively being embraced to offer students a practical exposure to industrial bioprocesses. Bioreactor computer simulators hold various advantages over conventional laboratory experiments, such as cost-effectiveness and enhanced safety. The research objective is to assess the effectiveness of integrating bioreactor computer simulators into hybrid teaching to promote active and collaborative learning experiences and evaluate their impact on student participation and understanding. A hybrid strategy combining synchronous, face-to-face, and online teaching has been implemented to enhance the teaching-learning processes in the Industrial Bioprocesses course for Biochemistry students. The simulation software BIOSTAT®T Yeast was used. This software models the production of ethanol with Saccharomyces cerevisiae through batch cultivation and the determination of the kLa value of a bioreactor. In the first simulation activity, students analyzed the software response based on parameter values input by the instructor, while in the second simulation activity, students autonomously used the computer simulator under the primary oversight of the instructor. The survey results indicate that the pedagogical innovation was positively received and significantly motivating for the students. Comparing student satisfaction surveys between the two simulation activities suggests that fostering student autonomy and engagement through simulation technology can improve satisfaction and learning outcomes in bioprocess education.","PeriodicalId":8830,"journal":{"name":"Biochemistry and Molecular Biology Education","volume":"52 5","pages":"519-526"},"PeriodicalIF":0.9,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140849657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"教育学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and assessment of a virtual escape-room game for teaching industrial bioprocesses 开发和评估用于工业生物工艺教学的虚拟逃生游戏

IF 0.9 4区教育学

Biochemistry and Molecular Biology Education Pub Date : 2024-05-04 DOI: 10.1002/bmb.21837

Davor Cotoras, Felipe Valenzuela-Ibaceta, Diego Salas, Franco Cárdenas

{"title":"Development and assessment of a virtual escape-room game for teaching industrial bioprocesses","authors":"Davor Cotoras, Felipe Valenzuela-Ibaceta, Diego Salas, Franco Cárdenas","doi":"10.1002/bmb.21837","DOIUrl":"10.1002/bmb.21837","url":null,"abstract":"This article presents a study on the implementation of a virtual escape-room game as a novel teaching methodology in biochemistry education. The game aimed to engage students in producing monoclonal antibodies against SARS-CoV-2 while reinforcing theoretical concepts and fostering teamwork. Three versions of the game were tested, incorporating modifications to address student feedback on and improve the overall experience. The study employed a satisfaction survey to gather insights from students regarding their perception of the game. Results showed that the implementation of answer flexibility using RegEx had a significant positive impact on student satisfaction and motivation. The introduction of RegEx allowed for a more realistic and immersive gaming experience, as students could provide varied answers while still being evaluated correctly. Overall, the findings highlight the effectiveness of the game's design, the suitability of the Google Forms platform for distance learning, and the importance of incorporating answer flexibility through RegEx. These results provide valuable guidance for educators seeking to enhance student engagement and satisfaction through the use of escape-room games in biochemistry education.","PeriodicalId":8830,"journal":{"name":"Biochemistry and Molecular Biology Education","volume":"52 4","pages":"453-461"},"PeriodicalIF":0.9,"publicationDate":"2024-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140830480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"教育学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0