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Biochemical Constituents of Phaleria macrocarpa (Leaf) Methanolic Extract Inhibit ROS Production in SH-SY5Y Cells Model 大丽(叶)甲醇提取物的生化成分抑制SH-SY5Y细胞模型中ROS的产生
IF 3
Biochemistry Research International Pub Date : 2020-11-29 DOI: 10.1155/2020/2640873
I. Hassan, Wan Norhamidah Wan Ibrahim, Ferdaus Binti Mohamat Yusuf, S. Ahmad, Syahida Ahmad
{"title":"Biochemical Constituents of Phaleria macrocarpa (Leaf) Methanolic Extract Inhibit ROS Production in SH-SY5Y Cells Model","authors":"I. Hassan, Wan Norhamidah Wan Ibrahim, Ferdaus Binti Mohamat Yusuf, S. Ahmad, Syahida Ahmad","doi":"10.1155/2020/2640873","DOIUrl":"https://doi.org/10.1155/2020/2640873","url":null,"abstract":"Background. Reactive oxygen species generation in mammalian cells profoundly affects several critical cellular functions, and the lack of efficient cellular detoxification mechanisms which remove these radicals may lead to several human diseases. Several studies show that ROS is incriminated as destructive agents in the context of the nervous system especially with advance in age leading to neurodegeneration. Current treatments of this disease are not effective and result in several side effects. Thus, the search for alternative medicines is in high demand. Therefore, the aim of this study is to evaluate the reactive oxygen inhibitory effect of Phaleria macrocarpa 80% (leaf) extract. Methods. The leaf was extracted with 80% methanol. Cytotoxicity studies were carried out using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and ROS inhibitory activities were evaluated using dichlorofluorescein diacetate (DCF-DA) assay in the SH-SY5Y cells model. Results. The result revealed ROS inhibitory activities of the crude extract with highly significant differences at \u0000 \u0000 p\u0000 <\u0000 0.001\u0000 \u0000 between the group that were treated with crude extract only, the group treated with crude extract and exposed to H2O2, and the group exposed to H2O2 only as well as the group that were maintained in complete media. Bioactive compounds show the presence of vitexin and isovitexin following the HPLC method. Conclusion. High antioxidant activities and low toxicity effect of this crude revealed its high benefit to be used as natural medicine/supplements.","PeriodicalId":8826,"journal":{"name":"Biochemistry Research International","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2020-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2020/2640873","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46472798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antibacterial, Antifungal, and Antidiabetic Effects of Leaf Extracts from Persea americana Mill. (Lauraceae) 美洲棕榈叶提取物的抗菌、抗真菌和抗糖尿病作用。(樟科)
IF 3
Biochemistry Research International Pub Date : 2020-11-11 DOI: 10.1155/2020/8884300
Mercy Makopa, Benjamin Mangiza, Benjamin Banda, Winnie Mozirandi, Molly Mombeshora, S. Mukanganyama
{"title":"Antibacterial, Antifungal, and Antidiabetic Effects of Leaf Extracts from Persea americana Mill. (Lauraceae)","authors":"Mercy Makopa, Benjamin Mangiza, Benjamin Banda, Winnie Mozirandi, Molly Mombeshora, S. Mukanganyama","doi":"10.1155/2020/8884300","DOIUrl":"https://doi.org/10.1155/2020/8884300","url":null,"abstract":"Fruits and leaves of Persia americana are used in traditional medical practices. This study was carried out to determine the antibacterial, antifungal, and antidiabetic effects of the leaf extracts from P. americana. The antibacterial activities of the leaf extracts were evaluated against Klebsiella pneumoniae and Staphylococcus epidermidis while antifungal activities were determined against Candida albicans and Candida tropicalis. The antidiabetic potential of the extracts was determined against mammalian α-glucosidase in vitro. The broth microdilution method was used to investigate the antibacterial and antifungal susceptibility of the microbial strains towards the leaf extracts. S. epidermidis was the most susceptible microbe out of the tested microorganisms. The acetone extract was the most potent extract against S. epidermidis with a minimum inhibitory concentration (MIC) of 50 μg/mL. At 100 μg/mL, the ethanol:water extract 18% of K. pneumoniae cells remained viable. Cell viability after exposure to the dichloromethane (DCM) and methanol extracts was 28% against C. albicans and 8% against C. tropicalis, respectively. The DCM:methanol and acetone extracts caused membrane damage in S. epidermidis exhibited by protein leakage. Only the acetone extract effected nucleic acid leakage. Screening of extracts’ potential to inhibit the activity of α-glucosidase was carried out spectrophotometrically following the production of p-nitrophenol from p-nitrophenol-glucopyranoside (substrate) at a wavelength of 405 nm. Out of all the tested extracts, the methanolic extract showed the best inhibitory activity on α-glucosidase enzyme in a time-dependent and dose-dependent manner.KiandKinactvalues were found to be 1.4 mg/mL and 2.4 U/min, respectively, after incubation for 1 hour. It was concluded that the leaf extracts of P. americana contain phytochemicals with antibacterial, antifungal, and α-glucosidase inhibitory effects. Further studies are required for the identification of the active compounds in the leaf extracts responsible for these observed effects.","PeriodicalId":8826,"journal":{"name":"Biochemistry Research International","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2020-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2020/8884300","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47819101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
In Vitro Determination of Antimicrobial and Hypoglycemic Activities of Mikania cordata (Asteraceae) Leaf Extracts 薇甘菊(菊科)叶提取物抑菌和降血糖活性的体外测定
IF 3
Biochemistry Research International Pub Date : 2020-11-10 DOI: 10.1155/2020/8674708
Pavithra L. Jayatilake, H. Munasinghe
{"title":"In Vitro Determination of Antimicrobial and Hypoglycemic Activities of Mikania cordata (Asteraceae) Leaf Extracts","authors":"Pavithra L. Jayatilake, H. Munasinghe","doi":"10.1155/2020/8674708","DOIUrl":"https://doi.org/10.1155/2020/8674708","url":null,"abstract":"Infectious diseases and diabetes mellitus are counted responsible for a substantial amount of mortality among the human population. The current study was performed to detect the antimicrobial activities and hypoglycemic potential of Mikania cordata (Asteraceae) leaves extracted into aqueous media and several organic solvents (ethyl acetate and methanol). The ethyl acetate extract of Mikania cordata (MEA) leaves was observed to possess significantly (\u0000 \u0000 p\u0000 ≤\u0000 0.05\u0000 \u0000 ) greater antimicrobial capabilities (susceptible against Bacillus cereus ATCC 11778 and Staphylococcus aureus ATCC 25923) when compared with that of the methanol (MME) and aqueous extracts (MDW) which were assessed based on Kirby-Bauer disk diffusion assay. The minimum inhibitory concentration of MEA (against B. cereus, S. aureus, and Escherichia coli ATCC 25922) and MME (against B. cereus, S. aureus, E. coli, and Candida albicans ATCC 10231) lies in a similar range of 1.13 > MIC>0.56 mg/ml. In the present study, a single compound (from MEA) of Rf value 0.64 was isolated by thin-layer chromatography (TLC) that was responsible for the zone of inhibition against B. cereus (20.3 ± 0.3 mm). The results of this study also depicted the antihyperglycemic properties of M. cordata leaves which followed the same trend as the commercial drug Metformin in a glucose concentration-independent manner when tested in a glucose uptake assay by yeast cells. Therefore, it is evident that Mikania cordata is a reservoir of useful bioactive compounds which with further research will be paving the path for drug commercialization. This is the first record of TLC-based isolation of antimicrobial compounds of M. cordata and analysis of the hypoglycemic properties of M. cordata leaves.","PeriodicalId":8826,"journal":{"name":"Biochemistry Research International","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2020-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2020/8674708","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42125091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Activity of Phosvitin in Hydroxyapatite Acid-Damage Immersion and Antimicrobial Assays. 羟基磷灰石酸损浸液中磷维素活性及抑菌试验。
IF 3
Biochemistry Research International Pub Date : 2020-10-24 eCollection Date: 2020-01-01 DOI: 10.1155/2020/8831311
Richard D Shipman, Sean D Doering, Jack R Hemsath, Eun Joo Lee, Jennifer E Grant
{"title":"Activity of Phosvitin in Hydroxyapatite Acid-Damage Immersion and Antimicrobial Assays.","authors":"Richard D Shipman,&nbsp;Sean D Doering,&nbsp;Jack R Hemsath,&nbsp;Eun Joo Lee,&nbsp;Jennifer E Grant","doi":"10.1155/2020/8831311","DOIUrl":"https://doi.org/10.1155/2020/8831311","url":null,"abstract":"<p><p>Phosvitin, the most highly phosphorylated metal-binding protein found in nature, binds more than 100 calcium ions, and has been identified as an agent that could be used to generate biomineralization scaffolds. Because of published reports describing phosvitin's affinity for calcium and potential antibiotic activity, this study was undertaken in order to evaluate phosvitin for both antibiotic activity against common microorganisms and the ability to protect hydroxyapatite surfaces from acid damage. To more clearly define its antibiotic action, the effects of phosvitin on <i>Micrococcus luteus</i>, <i>P. mirabilis</i>, <i>B. cereus</i>, <i>E. coli</i>, and <i>S. epidermidis</i> were evaluated. In both Kirby-Bauer tests and liquid culture growth inhibition assays, phosvitin inhibited <i>M. luteus</i>, a microorganism that thrives in the human mouth, but not the other bacteria tested. The MIC of phosvitin was determined to be 31.3 <i>μ</i>g/mL when delivered in 1 mM CaCl<sub>2</sub> but was 0.5 mg/mL in the absence of added calcium. Expanding on the potential impacts of phosvitin on the mouth, its action was evaluated in a model of tooth decay represented by acid-damaged hydroxyapatite discs. SEM, AFM, and FAAS analyses revealed that pretreatment of discs with phosvitin modulated the damage-induced morphology and topography changes associated with acid-damaged discs.</p>","PeriodicalId":8826,"journal":{"name":"Biochemistry Research International","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2020-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2020/8831311","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38683851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In Vitro Study on the Regulation of Annexin IV and VEGF by hCG in the Human Endometrium. hCG对人子宫内膜膜联蛋白IV和VEGF调控的体外研究。
IF 3
Biochemistry Research International Pub Date : 2020-10-23 eCollection Date: 2020-01-01 DOI: 10.1155/2020/8892930
Shaoyuan Xu, Jie Li, Xiaoyan Chen, Beiyu Liu
{"title":"In Vitro Study on the Regulation of Annexin IV and VEGF by hCG in the Human Endometrium.","authors":"Shaoyuan Xu,&nbsp;Jie Li,&nbsp;Xiaoyan Chen,&nbsp;Beiyu Liu","doi":"10.1155/2020/8892930","DOIUrl":"https://doi.org/10.1155/2020/8892930","url":null,"abstract":"<p><strong>Objective: </strong>Whether changes in vascular endothelial growth factor (VEGF) and annexin IV during implantation are regulated through the LH/hCG-R needs further research. To investigate the mechanism of hCG on the expression of annexin IV and VEGF in human endometrial cells.</p><p><strong>Methods: </strong>Endometrial cells were isolated and identified from human specimens. The proportion of glandular and epithelial cells was analyzed. Annexin IV and VEGF were analyzed by qRT-PCR (mRNA), western blot (proteins), and immunohistochemistry (proteins). Protein location was identified by immunohistochemistry. The cells were cultured with hCG, hCG/PD98059 (a MAPK inhibitor), or no treatment (control).</p><p><strong>Results: </strong>The proportions between the glandular epithelial cells and stromal cells at inoculation and when adding hCG were 25.8 ± 0.2% and 27.8 ± 0.04%, respectively (<i>P</i> > 0.05). LH/hCG-R, annexin IV, and VEGF were found in the cytoplasm of endometrial cells. After 2, 6, 12, and 24 h of hCG treatment, compared with 1 h, VEGF mRNA was increased by 1.25-fold, 3.19-fold, 4.21-fold, and 4.86-fold and annexin IV by 2.23-fold, 3.37-fold, 5.14-fold, and 5.02-fold. Compared with the control group, annexin IV mRNA and protein were increased in the hCG and hCG/PD98059 groups (mRNA/protein: 1.99-fold/1.80-fold and 2.33-fold/1.93-fold, <i>P</i> < 0.05). Compared with the control group, VEGF mRNA and protein were increased in the hCG group (mRNA/protein: 2.30-fold/1.86-fold), but not in the hCG/PD98059 group.</p><p><strong>Conclusion: </strong>hCG could upregulate the mRNA and protein expression of annexin IV and VEGF. The upregulation of annexin IV by hCG could not be inhibited by PD98059, but the upregulation of VEGF by hCG could.</p>","PeriodicalId":8826,"journal":{"name":"Biochemistry Research International","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2020-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2020/8892930","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38569394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Cognitive-Enhancing, Ex Vivo Antilipid Peroxidation and Qualitative Phytochemical Evaluation of the Aqueous and Methanolic Stem Bark Extracts of Lonchocarpus eriocalyx (Harms.). 长叶树(Lonchocarpus eriocalyx)茎皮水提物和醇提物的认知增强、体外抗脂质过氧化和定性植物化学评价[j]。
IF 3
Biochemistry Research International Pub Date : 2020-10-01 eCollection Date: 2020-01-01 DOI: 10.1155/2020/8819045
Gervason Moriasi, Anthony Ireri, Mathew Ngugi
{"title":"Cognitive-Enhancing, <i>Ex Vivo</i> Antilipid Peroxidation and Qualitative Phytochemical Evaluation of the Aqueous and Methanolic Stem Bark Extracts of <i>Lonchocarpus eriocalyx</i> (Harms.).","authors":"Gervason Moriasi, Anthony Ireri, Mathew Ngugi","doi":"10.1155/2020/8819045","DOIUrl":"10.1155/2020/8819045","url":null,"abstract":"<p><p>Over 50 million persons are living with cognitive deficits worldwide, with over 80% of these individuals living in the developing world. The number of affected persons is projected to go over 152 million by the year 2050. Current drugs used for cognitive impairment are debatably ineffective, costly, inaccessible, and associated with undesirable events that call for the search for alternative and complementary approaches. Plants are arguably affordable, accessible, and efficacious. However, despite the reported healing claims, scientific data validating these claims are lacking. <i>L. eriocalyx</i> is traditionally used for the management of various conditions, including cognitive impairment but has not been scientifically explored. In this study, the Morris Water Maze (MWM) method was used to evaluate <i>in vivo</i> cognitive-enhancing effects of studied extracts of <i>L. eriocalyx.</i> Furthermore, following MWM experiments, brains were dissected and processed, and malondialdehyde profiles were determined. Qualitative phytochemical profiles of the studied plant extracts were also determined. The results showed that mice that were treated with the studied plant extracts took significantly shorter transfer latencies, navigation distances, and significantly longer latencies in the target quadrant (NW) (<i>p</i> < 0.05) compared with the negative control mice, indicating cognitive-enhancing activities. Furthermore, cognitively impaired mice that received the studied plant extracts had significantly lower MDA profiles compared with the MDA profile of the negative control group mice (<i>p</i> < 0.05). The cognitive-enhancing and MDA profile lowering effects were attributed to the presence of antioxidant phytoconstituents that ought to have modulated the redox state, thereby attenuating brain damage. These extracts can be, therefore, used for the management of cognitive deficits. Further studies leading to isolation and characterization of active molecules for cognitive impairment are recommended. Furthermore, the precise mechanism(s) through which these extracts exert their pharmacologic activity should be established.</p>","PeriodicalId":8826,"journal":{"name":"Biochemistry Research International","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2020/8819045","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39079631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
Phenolic Content, Antioxidant Activity, Anti-Inflammatory Potential, and Acute Toxicity Study of Thymus leptobotrys Murb. Extracts. 胸腺酚含量、抗氧化活性、抗炎潜能及急性毒性研究。提取物。
IF 3
Biochemistry Research International Pub Date : 2020-09-18 eCollection Date: 2020-01-01 DOI: 10.1155/2020/8823209
Asmaa Oubihi, Hanae Hosni, Issmail Nounah, Abdessamad Ettouil, Hicham Harhar, Katim Alaoui, Mohammed Ouhssine, Zineb Guessous
{"title":"Phenolic Content, Antioxidant Activity, Anti-Inflammatory Potential, and Acute Toxicity Study of <i>Thymus leptobotrys</i> Murb. Extracts.","authors":"Asmaa Oubihi,&nbsp;Hanae Hosni,&nbsp;Issmail Nounah,&nbsp;Abdessamad Ettouil,&nbsp;Hicham Harhar,&nbsp;Katim Alaoui,&nbsp;Mohammed Ouhssine,&nbsp;Zineb Guessous","doi":"10.1155/2020/8823209","DOIUrl":"https://doi.org/10.1155/2020/8823209","url":null,"abstract":"<p><p><i>Thymus leptobotrys</i> is a medicinal plant belonging to the Lamiaceae family, endemic in Morocco, and used in traditional medicine. The present work aims to study the phenolic compounds, the antioxidant activity, the anti-inflammatory effect, and the toxicity of two ethanolic and methanolic extracts of <i>Thymus leptobotrys</i> aerial part. The yield of the methanolic extraction (22.2%) is higher than that of the ethanolic extraction (15.8%) and is characterized by higher contents of polyphenols 243.08 mg/g GAE (mg/g of gallic acid), flavonoids 179.28 mg/g RE (mg/g of rutin), and tannins 39.31 mg/g CE (mg/g of catechin). The <i>in vitro</i> measurement of antioxidant activity with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical reduction test and Trolox equivalent antioxidant capacity (TEAC) test demonstrates the higher performance of the methanolic extract. The evaluation of the anti-inflammatory effect <i>in vivo</i> on adult Wistar female rats leads to a very significant decrease in the inflammation of the edema compared to the standard drug (indomethacin) and the control group. The toxicity test reveals that both extracts showed no toxicity within an LD50 above 2000 mg/kg body weight of the rats.</p>","PeriodicalId":8826,"journal":{"name":"Biochemistry Research International","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2020-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2020/8823209","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38452350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 13
Physiochemical, Insecticidal, and Antidiabetic Activities of Senna occidentalis Linn Root. 西番泻根的理化、杀虫和降糖活性。
IF 3
Biochemistry Research International Pub Date : 2020-09-15 eCollection Date: 2020-01-01 DOI: 10.1155/2020/8810744
Tahani Osman Issa, Ahmed Ibrahim Mohamed Ahmed, Yahya Sulieman Mohamed, Sakina Yagi, Abdelrafie Mohamed Makhawi, Tarig Osman Khider
{"title":"Physiochemical, Insecticidal, and Antidiabetic Activities of <i>Senna occidentalis</i> Linn Root.","authors":"Tahani Osman Issa,&nbsp;Ahmed Ibrahim Mohamed Ahmed,&nbsp;Yahya Sulieman Mohamed,&nbsp;Sakina Yagi,&nbsp;Abdelrafie Mohamed Makhawi,&nbsp;Tarig Osman Khider","doi":"10.1155/2020/8810744","DOIUrl":"https://doi.org/10.1155/2020/8810744","url":null,"abstract":"<p><p>The present study aimed to investigate the physiochemical activities of <i>Senna occidentalis</i> (Linn) roots and phytochemicals as insecticidal (ethyl acetate and methanol) and antidiabetic (ethanolic extract) activities. Physicochemical properties were carried out by using Association of Official Analytical Chemist methods; thin layer chromatography was carried out according to the Stahl method. Larvicidal activity and LD<sub>50</sub> were studied against the third instar of <i>Culex quinquefasciatus</i> mosquito larvae to detect and extract toxicity. The ethanolic extracts of the roots were orally tested at the dose 200 mg/kg for the hypoglycemic effect on induced hyperglycemia in normal rats, assessed in the ethanol extract, and were compared with diabetic control and standards glibenclamide 10 mg/kg. Physiochemical parameters showed high rate in the nitrogen-free extract (69.6%), curd fiber (14.5%), crude proteins (8.15%), ether extract (3.75%), and both ash and moisture (2%), and high concentrations values were found in potassium (43 mg/l) followed by phosphorous (28.5 mg/l), calcium (15 mg/l), sodium (3.65 mg/l), and magnesium (0.145 mg/l). In this part, phytochemical compounds showed high amount of alkaloids, triterpene, flavonoids, tannins, sugars, and few amount of anthraquinone glycosides. Thin-layer chromatography (TLC) studies different colored phytochemical constituted with different Rf values. All the spots are colored under UV light, but some are localized colorless after spaying. The ethyl acetate (EtAc) extract showed eight spots, and the methanol (MeOH) extract showed thirteen spots. The larvicidal activity showed that the ethyl acetate extract was safe against mosquito larvae with an LD<sub>50</sub> value 1412.54 (<i>p</i> < 0.05), and the methanol extract had moderate larvicidal activity against mosquito larvae with an LD<sub>50</sub> value 257.54 (<i>p</i> < 0.05), while the ethanolic extract of <i>Senna occidentalis</i> (L.) causes a favorable hypoglycemic activity when compared to control significant reduction by [53.15%, 32.87%, and 20.94%], respectively, as well as standard glibenclamide. Based on the various data of the physicochemical parameters, TLC spots, and phytochemical compounds of <i>Senna occidentalis</i> root, they could be used as references standards for manufacturing units of <i>Senna occidentalis</i> root larvicidal and antidiabetic drugs.</p>","PeriodicalId":8826,"journal":{"name":"Biochemistry Research International","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2020-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2020/8810744","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38452349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Comparison between Conventional Decalcification and a Microwave-Assisted Method in Bone Tissue Affected with Mycetoma. 足菌肿骨组织常规脱钙与微波辅助脱钙的比较。
IF 3
Biochemistry Research International Pub Date : 2020-08-01 eCollection Date: 2020-01-01 DOI: 10.1155/2020/6561980
Magdi Mansour Salih
{"title":"Comparison between Conventional Decalcification and a Microwave-Assisted Method in Bone Tissue Affected with Mycetoma.","authors":"Magdi Mansour Salih","doi":"10.1155/2020/6561980","DOIUrl":"https://doi.org/10.1155/2020/6561980","url":null,"abstract":"<p><p>Mycetoma is a lifelong granulomatous disease of subcutaneous tissues and bones. Histopathology is a substantiated indicative method based on the assumption of a definitive diagnosis of mycetoma. It requires efficient processing of tissues including bone decalcification. The decalcification process must ensure complete removal of calcium and also a proper preservation of tissue and microorganisms' staining ability. <i>Objectives</i>. To compare the conventional method used in decalcification with the microwave method using different decalcification solutions. Different characteristics were tested, including the speed of decalcification and morphological and fungal preservation in bone tissue affected with mycetoma. <i>Materials and Methods</i>. Three decalcification solutions were employed to remove calcium from 50 bone tissue samples affected with mycetoma, including 10% neutral buffered EDTA (pH 7.4), 5% nitric acid, and 5% hydrochloric acid. Conventional and microwave methods were used. Haematoxylin-eosin (HE) stain, Gridley's stain, and Grocott hexamine-silver stain were employed to evaluate the bone and fungi morphologies. <i>Results</i>. The decalcification time of the conventional method compared with the microwave method with 10% EDTA (pH 7.4) took 120 hours and 29 hours, while 5% hydrochloric acid and 5% nitric acid took 8 hours and 3 hours, separately. Also, 10% EDTA is the best decalcifying agent for HE staining and fungal stains. 5% hydrochloric acid and 5% nitric acid can be used for fungal staining. <i>Conclusion</i>. The current study investigated the effects of different decalcifying agents as well as two decalcification procedures on the preservation of the bone structure and fungal staining, which will help to develop suitable protocols for the analyses of the bone tissue affected with mycetoma infection.</p>","PeriodicalId":8826,"journal":{"name":"Biochemistry Research International","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2020-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2020/6561980","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38300684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Optimization of Liquid Culture Condition of a Novel Fungus Hygrophoropsis sp. and Antioxidant Activity of Extracts. 新型真菌湿磷菌液体培养条件优化及提取物抗氧化活性研究。
IF 3
Biochemistry Research International Pub Date : 2020-08-01 eCollection Date: 2020-01-01 DOI: 10.1155/2020/7403257
Liang Huang, Chunxia Li, Ning Sun, Yu Wang, Hongpeng Yang, Yiting Li, Litong Ban
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