Biochemistry Research International最新文献

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Genome Mining and Structural Study of Cathelicidins Across Chiroptera Species. 翅目昆虫Cathelicidins的基因组挖掘与结构研究。
IF 3.4
Biochemistry Research International Pub Date : 2025-09-23 eCollection Date: 2025-01-01 DOI: 10.1155/bri/5461549
Manuel R López, Beatriz González-Almécija, Francisco Farrais-Solana, Andrea Otazo-Pérez, Sergio González-Acosta, Patricia Asensio-Calavia, Antonio Morales-delaNuez, José-Manuel Pérez de la Lastra
{"title":"Genome Mining and Structural Study of Cathelicidins Across Chiroptera Species.","authors":"Manuel R López, Beatriz González-Almécija, Francisco Farrais-Solana, Andrea Otazo-Pérez, Sergio González-Acosta, Patricia Asensio-Calavia, Antonio Morales-delaNuez, José-Manuel Pérez de la Lastra","doi":"10.1155/bri/5461549","DOIUrl":"10.1155/bri/5461549","url":null,"abstract":"<p><p>The authors explored the genomic landscape of bat cathelicidins, critical proteins in the vertebrate innate immune system, across 41 bat species, which are notable for their high microbial tolerance. An array of bioinformatics tools, complemented by manual mining, were used to identify the curated cathelicidin sequences. Particular attention was given to the fourth exon, which is responsible for generating the biologically active peptide. This comprehensive exploration led to the identification of 72 annotated complete cathelicidins, with 44 being newly discovered. These 72 complete cathelicidin sequences, along with 7 incomplete and 4 nonfunctional sequences, result in a total of 78 peptides, 53 of which were previously unknown. These bat cathelicidins are characterized by a cathelicidin domain pfam00666, and the consensus sequence derived from aligning these domains shows a high degree of conservation in the position of each amino acid across all bat species, suggesting that they possess only one type of cathelicidin. Notably, the three-dimensional structure of the bat cathelicidin family domain closely resembles the cystatin domain previously described in <i>Sus scrofa</i> protegrin 3. The phylogenetic tree constructed using the bat conserved domain cluster species according to their taxonomic order, indicating the potential utility of this sequence as taxonomic markers. Finally, the authors propose a peptide classification based on three-dimensional structures and the presence or absence of the CAP18 domain, proline-rich or arginine-rich sequences. This approach holds great promise for future research focussed on precisely identifying antimicrobial peptides, conducting structure-activity and mechanism-of-action relationship studies and enhancing our understanding of the immune defence mechanisms in bats.</p>","PeriodicalId":8826,"journal":{"name":"Biochemistry Research International","volume":"2025 ","pages":"5461549"},"PeriodicalIF":3.4,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12483743/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Study of Peripheral Blood T-Lymphocytes and Their Subpopulations in COVID-19 Patients. COVID-19患者外周血t淋巴细胞及其亚群的研究
IF 3.4
Biochemistry Research International Pub Date : 2025-09-15 eCollection Date: 2025-01-01 DOI: 10.1155/bri/5791950
Huaitai Lin, Jingxing Yi, Die Huang, Jichao Wu, Jun Yin
{"title":"Study of Peripheral Blood T-Lymphocytes and Their Subpopulations in COVID-19 Patients.","authors":"Huaitai Lin, Jingxing Yi, Die Huang, Jichao Wu, Jun Yin","doi":"10.1155/bri/5791950","DOIUrl":"10.1155/bri/5791950","url":null,"abstract":"<p><p><b>Objective:</b> To study the characteristic changes in peripheral blood T-lymphocytes and their subpopulations in COVID-19 patients by in-depth characterization of peripheral blood T-lymphocytes and to analyze the changes in the severity of COVID-19 and age factors in relation to changes in T-lymphocytes and their subpopulations. <b>Methods:</b> T-lymphocytes (including Th cells, regulatory T-lymphocytes, CD4+ initial T-lymphocytes, CD4+ memory T-lymphocytes, CD4+ T-lymphocytes functional subsets, Tc cells, CD8+ initial T-lymphocytes, CD8+ memory T-lymphocytes, and CD8+ T-lymphocyte functional subpopulations) were isolated, by flow cytometry, from peripheral blood specimens of 60 COVID-19 patients (experimental group) and 36 healthy controls (control group). The results of the two groups were compared and further analyzed in relation to age and disease severity of COVID-19 patients. <b>Results:</b> The absolute counts of T-lymphocytes and their subpopulations in the peripheral blood of COVID-19 patients were reduced, and the proportions of the cells in each subpopulation were imbalanced, with the absolute counts of T-lymphocytes and their subpopulations in peripheral blood of critically ill patients being lower relative to those of mildly ill patients, and the absolute counts of CD8+ initial T-lymphocytes in the peripheral blood of COVID-19 patients group being negatively correlated with the age of the patients. <b>Conclusion:</b> SARS-CoV-2 infection has an inhibitory effect on the number of T-lymphocytes and the proportion of their subpopulations, especially in critically ill and elderly patients, suggesting that SARS-CoV-2 infection has a serious impact on the cellular immune function of the body, and that in-depth characterization of T-lymphocyte population can more accurately reflect the changes in immune function to assess the state of cellular immune function.</p>","PeriodicalId":8826,"journal":{"name":"Biochemistry Research International","volume":"2025 ","pages":"5791950"},"PeriodicalIF":3.4,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12453925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145129969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Neuroprotective Effect of Resveratrol Propionate Esters on Apoptosis of SH-SY5Y Cells Induced by Hydrogen Peroxide. 白藜芦醇丙酸酯对过氧化氢诱导SH-SY5Y细胞凋亡的神经保护作用。
IF 3.4
Biochemistry Research International Pub Date : 2025-09-10 eCollection Date: 2025-01-01 DOI: 10.1155/bri/9973711
Chun-Yung Huang, Chih-Yao Hou, Shin-Yu Chen, Chang-Wei Hsieh, You-Lin Tain, Mei-Chun Lin, Yu-Wei Chen, Ming-Kuei Shih
{"title":"Neuroprotective Effect of Resveratrol Propionate Esters on Apoptosis of SH-SY5Y Cells Induced by Hydrogen Peroxide.","authors":"Chun-Yung Huang, Chih-Yao Hou, Shin-Yu Chen, Chang-Wei Hsieh, You-Lin Tain, Mei-Chun Lin, Yu-Wei Chen, Ming-Kuei Shih","doi":"10.1155/bri/9973711","DOIUrl":"10.1155/bri/9973711","url":null,"abstract":"<p><p>Resveratrol, often referred to as 3,4',5-trihydroxystilbene (RSV), is a compound with a variety of pharmacological benefits, such as antiaging, neuroprotective, chemopreventive, antioxidant, and anti-inflammatory qualities. To investigate neuroprotective properties of resveratrol propionate (RPE), H<sub>2</sub>O<sub>2</sub> was added to SH-SY5Y cells (human neuroblastoma cell line) that had been pretreated with RPE. The modulation of Bcl-2 and Bax proteins, the mitochondrial membrane potential (MMP), cytochrome c release, the activation of caspase-9 and caspase-3, DNA fragmentation, and membrane catenin analysis were all measured in this study using flow cytometry. The protective effect of RPE pretreatment of SH-SY5Y cells on the H<sub>2</sub>O<sub>2</sub>-induced death process was further investigated using annexin V-fluorescein isothiocyanate (FITC). The induction of caspase-9 and caspase-3, release of cytochrome c, loss of MMP, modification of Bcl-2 and Bax, DNA fragmentation, and annexin V-FITC/PI (propidium iodide) double staining all indicate that RPEs are capable of successfully shielding SH-SY5Y cells from H<sub>2</sub>O<sub>2</sub>-induced cytotoxicity. Therefore, RPE is considered a good candidate for the prevention and treatment of neurodegeneration induced by oxidative damage.</p>","PeriodicalId":8826,"journal":{"name":"Biochemistry Research International","volume":"2025 ","pages":"9973711"},"PeriodicalIF":3.4,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12443518/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145085157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Antarctic Wintering Alters the Properties of Human Plasma Cell-Free DNA. 南极的越冬改变了人类无浆细胞DNA的特性。
IF 3.4
Biochemistry Research International Pub Date : 2025-09-09 eCollection Date: 2025-01-01 DOI: 10.1155/bri/8994730
Sergey Ponomarev, Anastasia Kotikova, Nikolay Osetsky, Natalia Veiko, Elizaveta Ershova, Elena Malinovskaya, Ekaterina Savinova, Julia Chudakova, Julia Eliseeva, Vera Izhevskaia, Sergey Kutsev, Svetlana Kostyuk
{"title":"The Antarctic Wintering Alters the Properties of Human Plasma Cell-Free DNA.","authors":"Sergey Ponomarev, Anastasia Kotikova, Nikolay Osetsky, Natalia Veiko, Elizaveta Ershova, Elena Malinovskaya, Ekaterina Savinova, Julia Chudakova, Julia Eliseeva, Vera Izhevskaia, Sergey Kutsev, Svetlana Kostyuk","doi":"10.1155/bri/8994730","DOIUrl":"10.1155/bri/8994730","url":null,"abstract":"<p><p>Extracellular DNA of blood plasma (cell-free DNA, cfDNA) may potentially indicate a total level of apoptosis and mediate the immune response to stress induced by extreme environmental conditions, such as Antarctic wintering. We studied blood nuclease activity (NA); the content of 8-oxodG, rDNA, and SatIII(1q12) in cfDNA; and the levels of BAX, BCL2, TLR9, AIM2, STING, RIG-I, NF-kB, IL-8, and IL-17A mRNAs in 11 males, the members of the 64th Russian Antarctic Expedition. Blood was sampled before the wintering and on the 27th, 85th, 160th, 270th, and 315th days. The early months of the wintering are characterized by increased rates of apoptosis, an elevated BAX/BCL2 RNA ratio in blood leukocytes, and high cfDNA concentrations and NA in blood plasma. The properties of cfDNA are dramatically changed: the content of GC-rich rDNA rises, while AT-rich SatIII and 8-oxodG are low. We note individual multidirectional changes in the expression of <i>TLR9</i> and <i>AIM2</i>, while <i>STING</i> and <i>RIG-I</i> are downregulated in all of the subjects. The mRNA levels of <i>NFKB1</i>, <i>IL-8</i>, and <i>IL-17A</i> increase dramatically, indicating immune system activation. In conclusion, (1) apoptosis is overactivated and remains elevated during the first half of the Antarctic wintering; (2) cfDNA is enriched with GC-repeats, which stimulates its biological activity; and (3) the expression of immunity genes associated with the inflammatory response is increased.</p>","PeriodicalId":8826,"journal":{"name":"Biochemistry Research International","volume":"2025 ","pages":"8994730"},"PeriodicalIF":3.4,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12440658/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Serotonin-Mediated Avoidance and Immune Suppression in Caenorhabditis elegans Exposed to Sodium Arsenite. 暴露于亚砷酸钠的秀丽隐杆线虫血清素介导的回避和免疫抑制。
IF 3.4
Biochemistry Research International Pub Date : 2025-09-04 eCollection Date: 2025-01-01 DOI: 10.1155/bri/8485137
Xinyu Li, Jiaxin Zhao, Xiaoying Liu, Honglian Zhao, Xiaobing Zhang, Yaqi Deng, Qi Wang, Wei Zou
{"title":"Serotonin-Mediated Avoidance and Immune Suppression in <i>Caenorhabditis elegans</i> Exposed to Sodium Arsenite.","authors":"Xinyu Li, Jiaxin Zhao, Xiaoying Liu, Honglian Zhao, Xiaobing Zhang, Yaqi Deng, Qi Wang, Wei Zou","doi":"10.1155/bri/8485137","DOIUrl":"10.1155/bri/8485137","url":null,"abstract":"<p><p>Pathogen avoidance behavior is widespread across the animal kingdom and plays an important role in animal survival in natural environments. As a free-living nematode, <i>Caenorhabditis elegans</i> is exposed to various microorganisms and toxic chemicals in the soil, including pathogenic bacteria and chemical toxins. <i>C. elegans</i> can effectively avoid pathogenic bacteria, thereby minimizing the risk of infection. However, it remains unclear whether it also exhibits avoidance behavior in response to toxic substances such as sodium arsenite. In this report, we found that as the sodium arsenite concentration in the environment increased, the arsenic content in <i>C. elegans</i> also increased, leading to an avoidance response. Further investigation revealed that deletion of the serotonin synthesis gene <i>tph-1</i> significantly suppressed this avoidance behavior. Moreover, the expression level of TPH-1 protein in ADF neurons was significantly upregulated. Our data indicate that <i>C. elegans</i> exhibits a serotonin-mediated behavioral avoidance response to sodium arsenite. Additionally, we observed that the immune system of <i>C. elegans</i> was suppressed following sodium arsenite exposure, likely as an adaptation to the adverse environment. This study provides new insights into the strategies of <i>C. elegans</i> in response to toxic environmental conditions.</p>","PeriodicalId":8826,"journal":{"name":"Biochemistry Research International","volume":"2025 ","pages":"8485137"},"PeriodicalIF":3.4,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12425621/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Anti-Inflammatory and Analgesic Activities of the Saponin, Daucosterol, and the Triterpenoid Ester, β-Sitosterol 3-Myristate, From Capparis erythrocarpos (Isert) Capparaceae, and Their Interaction With the TRPV1 Ion Channel Transporter. 红辣椒科辣椒皂苷、桃甾醇和三萜酯β-谷甾醇3-肉豆蔻酸酯的抗炎镇痛活性及其与TRPV1离子通道转运体的相互作用
IF 3.4
Biochemistry Research International Pub Date : 2025-09-04 eCollection Date: 2025-01-01 DOI: 10.1155/bri/2621242
Emmanuel Kofi Kumatia, Nguyen Huu Tung, Alex Asase
{"title":"Anti-Inflammatory and Analgesic Activities of the Saponin, Daucosterol, and the Triterpenoid Ester, <i>β</i>-Sitosterol 3-Myristate, From <i>Capparis erythrocarpos</i> (Isert) Capparaceae, and Their Interaction With the TRPV1 Ion Channel Transporter.","authors":"Emmanuel Kofi Kumatia, Nguyen Huu Tung, Alex Asase","doi":"10.1155/bri/2621242","DOIUrl":"10.1155/bri/2621242","url":null,"abstract":"<p><p><i>Capparis erythrocarpos</i> is a medicinal plant traditionally used as an antiarthritic, anti-inflammatory, and analgesic agent. However, no previous reports exist on the analgesic and acute anti-inflammatory activities of the individual chemical constituents from this plant. This study reports the isolation, characterization, and evaluation of analgesic and acute anti-inflammatory activities of chemical constituents from the root bark of <i>C. erythrocarpos</i>, including their modulatory effects on TRPV1 ion channel activity. The isolated compounds were characterized as daucosterol (DC) and <i>β</i>-sitosterol 3-myristate (SM) using NMR and LC/GC-MS. DC significantly (<i>p</i> < 0.05) inhibited both phases of carrageenan-induced edema in a reverse dose-dependent manner, demonstrating 58.38% anti-inflammatory activity at 2 mg/kg p.o., comparable to diclofenac sodium (DS) at 8 mg/kg p.o. (53.07%). SM inhibited only phase 2 of carrageenan-induced edema. Both compounds significantly inhibited cold-induced pain with superior analgesic activity compared to DS. Against inflammation-induced pain, DC showed the highest analgesic activity (47.37% at 2 mg/kg p.o.). Molecular docking studies revealed that DC and SM produced ΔG values of -10.60 and -9.80 kcal/mol, respectively, which are more negative than those of DS (-8.6 kcal/mol), suggesting that they might be superior TRPV1 ion channel inhibitors and that DS likely has additional mechanisms of action. These results demonstrate that DC and SM possess remarkable therapeutic properties, warranting further exploration for novel drug development.</p>","PeriodicalId":8826,"journal":{"name":"Biochemistry Research International","volume":"2025 ","pages":"2621242"},"PeriodicalIF":3.4,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12425617/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effectiveness of Juniper Essential Oils in Reducing Selected Cigarette Smoke Toxicants, Improving Oxidative Parameters, and Cytotoxicity Against Lung Adenocarcinoma. 杜松精油在减少选定香烟烟雾毒物、改善氧化参数和抗肺腺癌细胞毒性方面的有效性。
IF 3.4
Biochemistry Research International Pub Date : 2025-08-17 eCollection Date: 2025-01-01 DOI: 10.1155/bri/6238789
Haouaouchi Fatma Zohra, Boudiba Sameh, Boudiba Louiza, Baya Berka, Karima Hanini, Gasmi Salim, Soraya Hioun, Alfred Ngenge Tamfu
{"title":"Effectiveness of Juniper Essential Oils in Reducing Selected Cigarette Smoke Toxicants, Improving Oxidative Parameters, and Cytotoxicity Against Lung Adenocarcinoma.","authors":"Haouaouchi Fatma Zohra, Boudiba Sameh, Boudiba Louiza, Baya Berka, Karima Hanini, Gasmi Salim, Soraya Hioun, Alfred Ngenge Tamfu","doi":"10.1155/bri/6238789","DOIUrl":"10.1155/bri/6238789","url":null,"abstract":"<p><p>Tobacco smoke contains toxic chemical substances that mediate the generation of reactive oxygen species and lung cancer. This study investigates the potential of essential oils (EOs) from <i>Juniperus oxycedrus</i> (JOX) and <i>Juniperus phoenicea</i> L. (JPH) in reducing the harmful effects of cigarette smoke. The EO of JOX (JOX-EO) and JPH (JPH-EO) showed good inhibition of nicotine, with IC<sub>50</sub> values of 16.78 ± 1.04 μg/mL and 18.40 ± 0.46 μg/mL, respectively. Moreover, JOX-EO had the greatest percentage reduction of basic tar (64.45%), followed by neutral tar (53.23%), and acidic tar (25.15%). Furthermore, the evaluated JOX-EO demonstrated efficacy in inhibiting polycyclic aromatic hydrocarbons (PAHs). In vivo experiments on rats chronically exposed to cigarette smoke reveal promising results regarding oxidative stress markers. The administration of JOX-EO at 200 mg/kg for 15 days postsmoking cessation shows significant improvements in hematological variables (red blood cells [RBC], platelets [PLT], hemoglobin [HB], hematocrit [HCT], and mean corpuscular volume [MCV]). The oxidative stress markers, including glutathione (GSH), malondialdehyde (MDA), glutathione S-transferase (GST), glutathione peroxidase (GPx), and catalase (CAT), were significantly reduced, indicating powerful antioxidant potential. The EOs exhibited concentration-dependent percentage inhibition of human lung carcinoma (A549) cell viability, with IC<sub>50</sub> values indicating greater potential than etoposide, the standard used for comparison. Both EOs demonstrate the capacity to mitigate the lingering effects of smoking, such as oxidative stress and lung cancer, providing reassurance and comfort to those affected.</p>","PeriodicalId":8826,"journal":{"name":"Biochemistry Research International","volume":"2025 ","pages":"6238789"},"PeriodicalIF":3.4,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12375859/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144940761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
DFT Investigations and Molecular Docking as Potent Inhibitors of SARS-CoV-2 Main Protease of Novel Pyrimidine Dione Derivatives. 新型嘧啶二酮衍生物作为SARS-CoV-2主蛋白酶抑制剂的DFT研究和分子对接。
IF 3.4
Biochemistry Research International Pub Date : 2025-08-11 eCollection Date: 2025-01-01 DOI: 10.1155/bri/7961294
Wesam S Shehab, Jalal Hasan Mohammed, Naja Magdy, Wael A Zordok, Mariusz Jaremko, Doaa A Elsayed
{"title":"DFT Investigations and Molecular Docking as Potent Inhibitors of SARS-CoV-2 Main Protease of Novel Pyrimidine Dione Derivatives.","authors":"Wesam S Shehab, Jalal Hasan Mohammed, Naja Magdy, Wael A Zordok, Mariusz Jaremko, Doaa A Elsayed","doi":"10.1155/bri/7961294","DOIUrl":"10.1155/bri/7961294","url":null,"abstract":"<p><p>The global outbreak of SARS-CoV-2 has emerged as a major public health crisis due to its rapid transmission and significant morbidity and mortality rates. In response, there is an urgent need to discover novel antiviral agents targeting key viral proteins. In this study, a new series of pyrimidine-2,4-dione derivatives was synthesized from barbituric acid and its thio analog, aiming to explore their potential inhibitory activity against the SARS-CoV-2 main protease (Mpro). The synthesis involved 1,4-addition and cyclodehydration reactions, yielding novel pyran and condensed pyrimidine derivatives. The chemical structures were confirmed using various spectroscopic techniques. Molecular docking studies were performed using MOE software (version 2022) targeting Mpro (PDB ID: 6LU7), revealing favorable binding affinities for several compounds. Compound 9 showed the best docking score (-12.70 kcal/mol), followed by Compound 4 (-12.38 kcal/mol) and Compound 11 (-12.13 kcal/mol), with key interactions involving residues such as Asn142, His41, and Glu166. DFT calculations were carried out to evaluate electronic properties, including energy gap (ΔE), global hardness (η), and softness (σ), indicating that Compound 10 was the most reactive with notable HOMO-LUMO characteristics. Additionally, the synthesized compounds were screened for drug-likeness based on Lipinski's rule of five and ADME parameters. Overall, the study identifies promising pyrimidine-based inhibitors of SARS-CoV-2 Mpro and provides valuable insights for further optimization and development of potential antiviral agents.</p>","PeriodicalId":8826,"journal":{"name":"Biochemistry Research International","volume":"2025 ","pages":"7961294"},"PeriodicalIF":3.4,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12360883/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144881973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hypoglycaemic Principles of Securidaca longepedunculata Extracts Reverses Experimental Diabetes and Diabetes Associated Pathology in Wistar Albino Rats. 长柄参提取物对Wistar白化大鼠实验性糖尿病及糖尿病相关病理逆转的降糖原理。
IF 3.4
Biochemistry Research International Pub Date : 2025-07-31 eCollection Date: 2025-01-01 DOI: 10.1155/bri/7173019
Obiora Emmanuel Abonyi, John Onyebuchi Ogbodo, Simeon Ikechukwu Egba, Innocent Ogheneovo Orhonigbe, Obioma Uzoma Njoku, Okwesili Fred Chiletugo Nwodo
{"title":"Hypoglycaemic Principles of <i>Securidaca longepedunculata</i> Extracts Reverses Experimental Diabetes and Diabetes Associated Pathology in Wistar Albino Rats.","authors":"Obiora Emmanuel Abonyi, John Onyebuchi Ogbodo, Simeon Ikechukwu Egba, Innocent Ogheneovo Orhonigbe, Obioma Uzoma Njoku, Okwesili Fred Chiletugo Nwodo","doi":"10.1155/bri/7173019","DOIUrl":"10.1155/bri/7173019","url":null,"abstract":"<p><p><b>Background:</b> This study investigated the effect of the extractable hypoglycaemic principles from <i>Securidaca longepedunculata</i> leaves on some biochemical parameters in alloxan-induced diabetic rats with the view to having improved pharmacological agent(s) that could be used in managing diabetes mellitus with relatively lesser side effects. Three groups of four rats each were used for the oral glucose tolerance test (OGTT): group one received 3 mL/kg BW normal saline (normal control) while groups 2 and 3 received 200 and 400 mg/kg BW of methanol extract of <i>Securidaca longepedunculata</i> (MESL), respectively. Six groups of five rats per group were used for the main antidiabetic study. Group one received 3 mL/kg BW normal saline (normal control), group 2 received no treatment after induction, group 3, received 5 mg/kg BW glibenclamide (standard group), groups 3-6 received 100, 200, and 400 mg/kg BW MESL, respectively, after induction. <b>Results:</b> The OGTT showed significant (<i>p</i> < 0.05) reduction in random blood sugar of the treated groups compared to the control. In the antidiabetic study, the treated groups 4-6 significantly (<i>p</i> < 0.05) lowered the blood glucose of the Wistar rats compared to the treatment control. Extract administration showed no adversity to liver and kidney, respectively, as shown by result of tested standard indicators. Equally MESL caused significant (<i>p</i> < 0.05) increase in serum antioxidant activities and significant (<i>p</i> < 0.05) decrease in malondialdehyde concentration compared to the treatment control. <b>Conclusion:</b> The study suggested hypoglycaemic potency, antioxidant rejuvenation, and relative hepatic and nephron safety by MESL.</p>","PeriodicalId":8826,"journal":{"name":"Biochemistry Research International","volume":"2025 ","pages":"7173019"},"PeriodicalIF":3.4,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12331390/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144798070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Functional Changes Associated With the Subcellular Localization of the Nuclear Receptor NR4A1. 核受体NR4A1亚细胞定位相关的功能改变。
IF 3.4
Biochemistry Research International Pub Date : 2025-07-24 eCollection Date: 2025-01-01 DOI: 10.1155/bri/4849733
Yoshimitsu Kiriyama, Akira Nakatsuma, Hiroshi Tokumaru, Hisayo Sadamoto, Hiromi Nochi
{"title":"Functional Changes Associated With the Subcellular Localization of the Nuclear Receptor NR4A1.","authors":"Yoshimitsu Kiriyama, Akira Nakatsuma, Hiroshi Tokumaru, Hisayo Sadamoto, Hiromi Nochi","doi":"10.1155/bri/4849733","DOIUrl":"10.1155/bri/4849733","url":null,"abstract":"<p><p>Nuclear receptor subfamily 4 group A member 1 (NR4A1), which is also known as nuclear receptor 77 (NUR77), NGFI-B, or testicular receptor 3 (TR3), is a member of the NR4A subfamily of the nuclear receptor superfamily. NR4A1 has both nuclear localization and nuclear export signals, and NR4A1 is present in the cytoplasm as well as the nucleus. NR4A1 alters its subcellular localization through phosphorylation or SUMOylation. In the nucleus, NR4A1 plays an important role in regulating gene expression by directly binding to genomic DNA or indirectly influencing other transcription factors. In the cytoplasm, NR4A1 affects the stabilization of β-catenin, which is involved in various tumorigenesis. Furthermore, NR4A1 is involved in LPS-induced inflammasome activation. In mitochondria and endoplasmic reticulum, NR4A1 plays an important role in the induction of autophagy and apoptosis. In this review, we focused on the current knowledge of subcellular localization and molecular function of NR4A1.</p>","PeriodicalId":8826,"journal":{"name":"Biochemistry Research International","volume":"2025 ","pages":"4849733"},"PeriodicalIF":3.4,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12313389/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144759035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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