Biochemistry Research International最新文献

{"title":"Diversity of Ethiopian Black Cumin (<i>Nigella sativa</i> L.) Based on Compositions of Essential Oil.","authors":"Basazinew Degu, Bizuayehu Tesfaye, Wendawek Abebe, Kebebew Assefa","doi":"10.1155/bri/2065593","DOIUrl":"https://doi.org/10.1155/bri/2065593","url":null,"abstract":"<p><p>Black cumin (<i>Nigella sativa</i> L.) seeds essential oil compositions (EOCs) have been used for their medicinal and aromatic values across the world since ancient times. Studies have revealed the presence of variability among black cumin genotypes in EOCs. In Ethiopia, few studies have been conducted to explore the variability of black cumin genotypes by using EOCs. This study investigated the variability of Ethiopian black cumin genotypes (EBCGs) by EOCs. Seeds of 64 <i>N. sativa</i> genotypes were used for this experiment. Composite samples of 100 g of seeds were collected and roughly ground from each genotype. The extraction was made by hydrodistillation using a Clevenger-type apparatus for 3 h, and the essential oil was collected by measuring the amount using a measuring pipette. The essential oil samples were stored in a refrigerator at 4°C until gas chromatography-mass spectrometry (GC-MS) analysis. Descriptive statistics was used to estimate the variations among populations' combined mean values of EOCs using the SAS version 9.4 software package. The correlation and hierarchical clustering analysis were made based on the combined mean values of EOCs using the R-software version 4.2.2 packages. A total of 21 EOCs were detected from the essential oil of 64 EBCGs using GC-MS, out of which ρ-cymene, thymoquinone, α-thujene, carvacrol, <i>trans</i>-4-methoxythujane, longifolene, terpinen-4-ol, β-pinene, α-pinene, and <i>d</i>-limonene dominated the essential oils. Among these, ρ-cymene, thymoquinone, α-thujene, <i>trans</i>-4-methoxythujane, and carvacrol were the most abundant constituents in all genotypes, while the rest varied among the genotypes. It is predicted that the major EOCs will be improved by 25.33%-152.14% over improved varieties by selecting the top 5% of landraces. The abundant EOC thymoquinone had a significant and positive correlation with carvacrol and a strong and significant negative correlation with α-thujene, α-pinene, β-pinene, ρ-cymene, and <i>d</i>-limonene. Based on the major EOCs, cluster analysis grouped the 64 genotypes into two different chemotypes. Cluster-I: Chemotype A is characterized by a high content of thymoquinone. Cluster-II: Chemotype B is characterized by a high content of ρ-cymene. The presence of the most abundant volatile constituents in genotypes 242835, 9068, and 014_ATH means they are essential for the pharmaceutical and food industries. This study disclosed the existence of a significant diversity of EOCs among the Ethiopian <i>N. sativa</i> genotypes, which can be exploited for future improvement programs.</p>","PeriodicalId":8826,"journal":{"name":"Biochemistry Research International","volume":"2025 ","pages":"2065593"},"PeriodicalIF":3.4,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12052447/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current Understanding of Feather Keratin and Keratinase and Their Applications in Biotechnology.","authors":"Thanakorn Moktip, Lakha Salaipeth, Ana Eusebio Cope, Mohammad J Taherzadeh, Takashi Watanabe, Paripok Phitsuwan","doi":"10.1155/bri/6619273","DOIUrl":"https://doi.org/10.1155/bri/6619273","url":null,"abstract":"<p><p>The food industry generates substantial keratin waste, particularly chicken feathers, which are rich in amino acids and essential nutrients. However, the insolubility of keratin presents a significant challenge to its conversion. Keratinase, an enzyme produced by certain fungi and bacteria, offers a promising solution by degrading feather keratin into amino acids and soluble proteins. Among these, bacterial keratinase is notable for its superior stability and activity, although its production remains constrained, necessitating continued research to identify efficient microbial strains. Keratin-derived hydrolyzates, recognized for their biological and immunological properties, have garnered significant research interest. This review examines the structural characteristics of chicken feather keratin, its resistance to conventional proteases, and advances in keratinase production and purification techniques. Additionally, the keratin degradation mechanism and the adoption of environmentally friendly technologies for managing feather waste are explored. Finally, the review highlights the potential applications of keratinase across diverse industries, including animal feed and cosmetics.</p>","PeriodicalId":8826,"journal":{"name":"Biochemistry Research International","volume":"2025 ","pages":"6619273"},"PeriodicalIF":3.4,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041636/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical and Bioactive Analysis of Extracted Brown Macroalgae (<i>Dictyota implexa</i>) Collected in Vietnam.","authors":"Nguyen Ngoc Trang Thuy, Tran Thanh Men","doi":"10.1155/bri/9461117","DOIUrl":"10.1155/bri/9461117","url":null,"abstract":"<p><p>Brown algae are considered a marine algae resource for human health. This study investigated ethanol extract's chemical composition and biological activity from brown algae <i>Dictyota implexa.</i> The extract from <i>D. implexa</i> was examined for total contents of quercetin, tannic acid, phenolic, flavonoid, polysaccharides, agar, and fucoidan, and the antioxidant, anti-inflammatory, antibacterial, cytotoxic, and α-amylase inhibitory activities of the crude extract were determined. Results revealed the presence of a source of phenolic (85.95 ± 1.21 mg GAE/g of the sample), flavonoid (245.6 ± 2.83 mg QE/g of the sample), and tannin (172.179 mg/g DW) compounds in the extract. Evaluating antioxidant activity proved the ethanol extract of <i>D. implexa</i> possessed the highest activity on two testing methods of DPPH scavenging capacity and reducing power. Besides, the anti-inflammatory activity was potent in the extract with an IC₅₀ value of 9.95 ± 1.51 μg/mL. Concerning antimicrobial activities, the ethanol extract of <i>D. implexa</i> (70 mg/mL) showed potential inhibitory ability against <i>E. coli</i> and <i>B. cereus</i>. Moreover, the algal extract displayed cytotoxic activity against HeLa cells and inhibited α-amylase activity with an IC₅₀ value of 276.82 μg/mL. The current findings demonstrated that exploring novel natural resources offers a promising avenue for advancements in human health and economic well-being.</p>","PeriodicalId":8826,"journal":{"name":"Biochemistry Research International","volume":"2025 ","pages":"9461117"},"PeriodicalIF":3.4,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11972136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Some Long Noncoding RNAs (LncRNAs) in Pediatric Inflammatory Bowel Disease (IBD): An Iranian Study.","authors":"Fatemeh Khesali, Azizollah Yousefi, Seyyed Amir Yasin Ahmadi, Reza Nekouian","doi":"10.1155/bri/8879418","DOIUrl":"10.1155/bri/8879418","url":null,"abstract":"<p><p><b>Introduction:</b> According to the importance of long noncoding RNAs (LncRNA) in the pathogenesis of inflammatory bowel disease (IBD) and also the lack of study for pediatric IBD in this regard, we investigated the expression of a selected panel of LncRNAs in Iranian pediatric cases of IBD compared to adult cases and healthy samples. <b>Methods:</b> In this gene expression study, blood samples were taken from the three groups of pediatric IBD cases, adult IBD cases, and pediatric healthy samples (for gene expression calibration). The investigated LncRNAs were <i>UCA1</i>, <i>CCAT</i>, <i>IFNG-AS1</i>, and <i>CDKN2B</i>. Real-time PCR was used and fold changes (FCs) were reported. <b>Results:</b> A total of 50 individuals were studied including 28 cases of pediatric IBD, 12 cases of controls, and 10 cases of adult IBD. <i>UCA1</i> showed upregulation in adult IBD (FC = 10.56, <i>p</i> = 0.007). <i>CCAT</i> showed downregulations for pediatric IBD (FC = 0.01, <i>p</i> < 0.001) and adult IBD (FC = 0.10, <i>p</i> = 0.039). <i>IFNG-AS1</i> showed downregulation in pediatric IBD (FC < 0.01, <i>p</i> < 0.001). CDKN2B showed upregulation in pediatric IBD (FC = 17.39, <i>p</i> < 0.001). The results were in contrast with the literature. <b>Conclusion:</b> It seems that these LncRNAs may have different roles in pediatric IBD. Further studies are needed on pediatric cases of IBD.</p>","PeriodicalId":8826,"journal":{"name":"Biochemistry Research International","volume":"2025 ","pages":"8879418"},"PeriodicalIF":3.4,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11972125/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Urinary Screening for Aminoacidurias Using Chromatography and Serum Amino Acid Profile in Type 2 Diabetes and Healthy Controls.","authors":"Sushma B J, Sumit Parashar, Balvir Singh Tomar, Alka Meena, Priyanka B J","doi":"10.1155/bri/4060832","DOIUrl":"10.1155/bri/4060832","url":null,"abstract":"<p><p>Type 2 diabetes is a complex metabolic disorder characterised by altered amino acid metabolism. This study investigates plasma amino acid profiles and specific aminoacidurias in patients with type 2 diabetes. We recruited 115 patients with type 2 diabetes and 115 healthy controls. Urine amino acids were analysed using paper chromatography, while serum amino acids were analysed using gas chromatography-mass spectrometry (GC-MS). Diabetes-induced alterations in amino acid metabolism are multifaceted. Hyperglycemia and insulin resistance can lead to increased gluconeogenesis, resulting in the depletion of certain amino acids, such as glutamine and arginine. Conversely, the increased availability of branched-chain amino acids (BCAAs) such as leucine, isoleucine and valine can contribute to insulin resistance and impaired glucose metabolism. Significant alterations in plasma and urine amino acid levels were observed in patients with type 2 diabetes, including increased levels of BCAAs and decreased levels of glutamine and arginine. These changes correlated with disease severity and metabolic parameters. Understanding these alterations is crucial for developing novel therapeutic strategies and diagnostic tools for type 2 diabetes management. Our findings provide new insights into amino acid metabolism in type 2 diabetes, identifying potential biomarkers for disease diagnosis and monitoring. These results suggest avenues for developing therapeutic strategies and contribute to understanding the complex relationship between amino acid metabolism and type 2 diabetes. The study highlights the potential of amino acid profiling as a diagnostic and therapeutic tool in type 2 diabetes management.</p>","PeriodicalId":8826,"journal":{"name":"Biochemistry Research International","volume":"2025 ","pages":"4060832"},"PeriodicalIF":3.4,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11932744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"L-Arginine Activates the Neuregulin-1/ErbB Receptor Signaling Pathway and Increases Utrophin mRNA Levels in C2C12 Cells.","authors":"Gladys Tapia, Sebastián Fuenzalida, Constanza Rivera, Pía Apablaza, Mónica Silva, Enrique Jaimovich, Nevenka Juretić","doi":"10.1155/bri/2171745","DOIUrl":"https://doi.org/10.1155/bri/2171745","url":null,"abstract":"<p><p>L-arginine induces the expression of utrophin in skeletal muscle cells, so it has been proposed as a pharmacological treatment to attenuate the symptoms of Duchenne muscular dystrophy (DMD). On the other hand, it has been described that one of the pathways that participates in the expression of utrophin in muscle is the Neuregulin-1 (NRG-1)/ErbB receptors pathway. Several studies have postulated that disintegrin and metalloprotease-17 (ADAM17) causes the proteolytic processing of NRG of transmembrane, allowing the release of NRG to the medium, which when joining its ErbB receptor activates the signaling pathway that triggers utrophin transcription. The aim of this study was to evaluate the effect of L-arginine in the activation of NRG-1/ErbB pathway and utrophin mRNA levels in C2C12 cells, and the participation of ADAM17 in this process. Our results indicate that L-arginine induces phosphorylation of ErbB2 and increases utrophin mRNA levels in C2C12 myotubes, with a maximum increase of 2-fold at 4 h post-stimulation. This effect is not observed when the myotubes are stimulated in the presence of GM6001 (general metalloprotease inhibitor) or PD-158780 (specific inhibitor of ErbB receptor phosphorylation). Experiments performed by flow cytometry suggest that L-arginine stimulates ADAM17 activation in our study model. Furthermore, immunofluorescence analysis supports our findings that L-arginine stimulates ADAM17 increase in treated myotubes. However, our results using pharmacological inhibitors suggest that ADAM17 does not participate in utrophin expression in C2C12 cells treated with L-arginine. The results obtained help to clarify the mechanism of action of L-arginine in the expression of utrophin in muscle cells, which will contribute to the design of new therapeutic strategies in pathologies such as DMD.</p>","PeriodicalId":8826,"journal":{"name":"Biochemistry Research International","volume":"2025 ","pages":"2171745"},"PeriodicalIF":3.4,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11991828/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143968440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical Characterization and Antimicrobial Properties of Extracts From Four Food Plants Traditionally Used to Improve Drinking Water Quality in Rural Areas of Burkina Faso.","authors":"Frédéric Anderson Konkobo, Poussian Raymond Barry, Sandrine Zongo, Elisabeth Rakisewendé Ouédraogo, Noëlle Edwige Roamba, Roger Dakuyo, David Bazié, Balamoussa Santara, Mamounata Diao, Paul Windinpsidi Savadogo, Mamoudou Hama Dicko","doi":"10.1155/bri/9994531","DOIUrl":"10.1155/bri/9994531","url":null,"abstract":"<p><p>The use of local plant extracts in drinking water purification represents a sustainable alternative in the fight against diseases linked to unsafe water consumption in rural areas. The aim of this study was to evaluate the biochemical composition and antimicrobial activity of four local plant extracts used in rural areas of Burkina Faso to purify drinking water: <i>Moringa oleifera</i> seeds, <i>Boscia senegalensis</i> seeds, <i>Opuntia ficus-indica</i> cladodes, and <i>Aloe vera</i> leaves. These four extracts were then subjected to biochemical screening to identify phytocompounds, followed by quantification and evaluation of their antibacterial properties on ten pathogenic bacterial strains. The screening results revealed the presence of a variety of molecules (phenolic compounds, alkaloids, saponosides, etc.) in the different extracts studied. From a quantitative point of view, <i>M. oleifera</i> and <i>B. senegalensis</i> seeds showed a high total protein content (34.5 and 24.6 g/100 g DM). <i>A. vera</i> and <i>O. ficus-indica</i> extracts showed high levels of total carbohydrates (20.4 and 35.52 g/100 g DM) compared with total lipids and proteins. The same applies to phenolic compounds, which were also high in <i>A. vera</i> and <i>O. ficus-indica</i> extracts (17.42 and 26.5 mg GAE/100 mg DM) compared to <i>M. oleifera</i> and <i>B. senegalensis</i> seeds. In terms of antibacterial properties, the four extracts studied showed inhibition diameters ranging from 7.33 to 16.33 mm. These results reflect the ability of the different extracts to eliminate pathogenic microorganisms present in water. Overall, this study stands out for its innovative character, offering an in-depth understanding of the biochemical composition and antimicrobial properties of four distinct extracts of local plants commonly used in traditional drinking water purification practices. It enriches existing knowledge by providing new data on the biochemical composition and bioactivity of these extracts. In particular, the study highlights the synergistic effects of the bioactive compounds present, underlining their essential role in improving the sanitary quality of water consumed in rural areas, where sustainable and accessible solutions are crucial.</p>","PeriodicalId":8826,"journal":{"name":"Biochemistry Research International","volume":"2025 ","pages":"9994531"},"PeriodicalIF":3.4,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11858832/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143498233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential Mechanisms of Lactate Dehydrogenase and Bovine Serum Albumin Proteins as Antioxidants: A Mixed Experimental-Computational Study.","authors":"Jing Ye, Amy Bounds, Madeline Crumpton, Mallory Long, Haley McDonough, Isabella Srikhirisawan, Shanzhen Gao","doi":"10.1155/bri/9638644","DOIUrl":"10.1155/bri/9638644","url":null,"abstract":"<p><p>Proteins have shown varying degrees of antioxidant activity. This study examined the potential mechanisms of interactions between proteins and radicals using chemical kinetics and computational methods. The study quantified the antioxidant activity of lactate dehydrogenase (LDH) and bovine serum albumin (BSA) through Trolox equivalent antioxidant capacity (TEAC) and oxygen radical absorbance capacity (ORAC) assays. BSA was about seven times and LDH 12 times more potent as antioxidants for 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS^•-) than they were for peroxyl radicals. According to the evaluation of Trolox equivalents (TE) of 20 proteinogenic amino acids, tryptophan (with a TE value of 101 μmol TE/μmol) exhibited the highest antioxidant activity for ABTS^•-, followed by tyrosine (38.7 μmol TE/μmol) and cysteine (30.5 μmol TE/μmol), lysine (0.193 μmol TE/μmol), arginine (0.0325 μmol TE/μmol), valine (0.0280 μmol TE/μmol), histidine (0.00689 μmol TE/μmol), and leucine (0.00560 μmol TE/μmol). The EC50 showed a similar order with a swap between valine and histidine. The antioxidant activity of the amino acids and proteins was temperature dependent. The rate laws, activation energy, and pre-exponential factor A of these reactions provided information on the reaction mechanisms, i.e., a biomolecular elementary step for the reaction of ABTS^•- with amino acids tryptophan, tyrosine, cysteine, or protein LDH, and a more complicated mechanism for BSA. The presence of -NH- or hydroxyl groups on aromatic rings enhanced the antioxidant ability of tryptophan and tyrosine. LDH's antioxidant activity did not affect its enzymatic activity, indicating that the radical reaction likely happened on the protein's surface without significantly altering its conformation. The molecular modeling and visualization showed potential reaction sites on the proteins' accessible tryptophan and tyrosine residues. However, the mere surface exposure of tryptophan and tyrosine does not guarantee their antioxidant activities.</p>","PeriodicalId":8826,"journal":{"name":"Biochemistry Research International","volume":"2025 ","pages":"9638644"},"PeriodicalIF":3.4,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11832265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Propolis on PPP2R1A and Apoptosis in Cancer Cells.","authors":"Burak Durmaz, Latife Merve Oktay Çelebi, Ayşe Çekin, Ayshan Ahadova, Nur Selvi Günel, Hatice Kalkan Yıldırım, Ali Mert Özgönül, Eser Yıldırım Sözmen","doi":"10.1155/bri/5538068","DOIUrl":"10.1155/bri/5538068","url":null,"abstract":"<p><p>Recently, it has been shown that protein phosphatase 2A (PP2A) dysfunction was common in many cancer types and was mediated by various inactivation mechanisms. Although many research studies observed antitumor effect of propolis extracts in various types of cancer, the mechanism of effect are still obscure. In this study, we investigated the effect of propolis on PPP2R1A expression and its relationship with apoptosis in the SW-620 (colorectal cancer), DU-145 and PC-3 (prostate cancer), and MCF-7 (breast cancer) cell lines, with WI-38 (healthy fibroblast) cells serving as the control. Moreover, we aimed to investigate the impact of propolis on apoptosis by analyzing apoptosis markers such as tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), APAF-1, and caspases-3, -8, and -9. Propolis samples were extracted, and their phenolic compounds were quantified using LC-MS/MS. The RealTime Cell Analysis System-xCELLigence (RTCA-SP) device and software were employed to assess cell viability and cytotoxicity of the propolis samples. The IC₅₀ values for propolis were determined (298 μg/mL for SW-620, 185.6 μg/mL for DU-145, 250.7 μg/mL for PC - 3, 292.9 μg/mL for MCF-7, and 311.2 μg/mL for WI-38). Subsequently, the effects of propolis on PPP2R1A expression and apoptosis markers (TRAIL, Apaf-1, and caspases-3, -8, and -9) were analyzed. When we compared the healthy cell lines to cancer cell lines, a statistically significant increase in caspase-3 (3.62-fold) and in TRAIL (4.38-fold) was observed in the SW-620 cell line after the application of propolis. In addition, in the PC-3 cell line, a 1.4-fold increase in caspase-8 was observed compared with the healthy cell line, which is also statistically significant. Our findings indicated that propolis increased the PPP2R1A levels and apoptosis markers in cancer cell lines. It has been suggested that high PPP2R1A levels induced by propolis treatment might activate the apoptosis pathway. In this study, the inducible effect of propolis on PPP2R1A levels, identified as a new target for cancer treatment, was demonstrated for the first time. The findings suggest that propolis holds promise as a potential cancer therapy by increasing PPP2R1A levels, a key molecule in cancer treatment.</p>","PeriodicalId":8826,"journal":{"name":"Biochemistry Research International","volume":"2025 ","pages":"5538068"},"PeriodicalIF":3.4,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11756940/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current Perspectives in Detection of Bioactive Compounds From <i>Costus spicatus</i> Through GC-MS and LC-MS/MS: Antioxidant Properties and In Silico Analysis for Industrial Applications.","authors":"Dharmar Manimaran, Ararsa Tessema, M Govindarajulu Yadav, S Parthasarathi, Vasan Palanisamy","doi":"10.1155/bri/9599942","DOIUrl":"10.1155/bri/9599942","url":null,"abstract":"<p><p>Members of the <i>Costus</i> genus are the conventional medicinal plants used in the therapeutic management of numerous ailments, especially for their antioxidant and pharmacological activities. The crude extract of <i>Costus spicatus</i> was profiled using high-resolution GC-MS and LC-MS/MS techniques to determine possible bioactive compounds that are vital to the antioxidant activity. A total of 52 and 63 bioactive compounds have been detected in GC-MS chromatograms using different solvents (methanol and ethanol) in <i>C. spicatus</i> leaf extracts, representing the presence of certain bioactive compounds. The identified bioactive compounds in both extracts which exhibit neuroprotective effects have been confirmed through various literature studies. They are cholestan-3-amine, loliolide, stigmasterol and methylprednisolone acetate, succinimide, fumaric acid, beta-tocopherol and gamma-tocopherol. The aqueous extract possessed the highest antioxidant activity for DPPH scavenging activity and lipid peroxidation inhibition assays, whereas the alcoholic aqueous extract showed superior efficacy for hydroxyl radical scavenging activity. In this study, we performed molecular docking and found that four compounds exhibited promising binding affinities with predicted binding sites on alpha-synuclein. Notably, Androsta [17-16-b] furan-5'-imine, 4'-methylene-3-methoxy-N-cyclohexyl- showed the highest docking interaction score of -7.4, indicating a strong binding affinity. These findings, combined with the presence of bioactive components in the crude extract of <i>C. spicatus</i>, suggest that this plant may possess neuroprotective properties, warranting further investigation for potential industrial applications in the development of neuroprotective agents.</p>","PeriodicalId":8826,"journal":{"name":"Biochemistry Research International","volume":"2025 ","pages":"9599942"},"PeriodicalIF":3.4,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11753854/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}