Biocatalysis and Biotransformation最新文献_第8页

Bioremediation mediated by manganese peroxidase – An overview 锰过氧化物酶介导的生物修复——综述

IF 1.8 4区生物学

Biocatalysis and Biotransformation Pub Date : 2022-08-25 DOI: 10.1080/10242422.2022.2113517

Shilpa Saikia, M. Yadav, Rohida Amin Hoque, H. S. Yadav

引用次数: 4

Biodegradation and detoxification of textile dyes using a novel bacterium Bacillus sp. AS2 for sustainable environmental cleanup 新型细菌芽孢杆菌AS2对纺织染料的生物降解和解毒作用

IF 1.8 4区生物学

Biocatalysis and Biotransformation Pub Date : 2022-08-23 DOI: 10.1080/10242422.2022.2113518

Anita Rani Santal, R. Rani, Anil Kumar, J. Sharma, N. Singh

引用次数: 3

Chloroperoxidase applications in chemical synthesis of industrial relevance 氯过氧化物酶在化学合成中的应用具有工业意义

IF 1.8 4区生物学

Biocatalysis and Biotransformation Pub Date : 2022-08-09 DOI: 10.1080/10242422.2022.2107919

Y. Bhandari, Hemlata Sajwan, Parul Pandita, V. Koteswara Rao

{"title":"Chloroperoxidase applications in chemical synthesis of industrial relevance","authors":"Y. Bhandari, Hemlata Sajwan, Parul Pandita, V. Koteswara Rao","doi":"10.1080/10242422.2022.2107919","DOIUrl":"https://doi.org/10.1080/10242422.2022.2107919","url":null,"abstract":"Abstract Biocatalysts can accelerate the catalysis of a chemical reaction that is difficult to synthesize with typical chemical methods. The global enzyme market size is predicted to expand at a CAGR of 6.5% from 2021 to 2028. Enzymatic reactions are highly chemo, regio, and stereoselective and produce various fine chemicals such as drugs, agrochemicals, and fragrance molecules. Peroxidases (PO) (EC 1.11.1.x) are a large class of enzymes that play an important role in various biological processes. Chloroperoxidase (CPO, EC 1.1.1.10) is a versatile fungal haem-thiolate protein that is useful in the asymmetric synthesis of chiral building blocks and has an important role in a number of biological processes. CPO’s main biological role is chlorination, although it also catalyses haem PO, catalase (CAT), and reactions similar to cytochrome P450. However, CPO performs both oxidation and stereo-specific halogenation of chemical molecules. The haem and vanadium POs are produced by Caldariomyces fumago, and Curvularia inaequalis, respectively, and are capable of halogenating the flavanones, naringenin, and hesperetin, at C-6 and C-8 in the presence of either Cl− or Br−. In this review, we discussed the various applications of CPO including synthesis of epoxides, drugs, halogenation of thymol, nitriles, the Aza-Achmatowicz reaction, and biomedical applications such as cancer and biosensors. In light of these novel features, we have provided a detailed review of CPOs and their applications in various stereoselective chemical transformations of industrial relevance.","PeriodicalId":8824,"journal":{"name":"Biocatalysis and Biotransformation","volume":"41 1","pages":"403 - 420"},"PeriodicalIF":1.8,"publicationDate":"2022-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47184745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Biomass suspension catalysed the generation of various alkyl esters from acid oil and virgin cottonseed oil 生物质悬浮液催化酸性油和棉籽油生成各种烷基酯

IF 1.8 4区生物学

Biocatalysis and Biotransformation Pub Date : 2022-08-04 DOI: 10.1080/10242422.2022.2107427

Anirudh Sharma, J. Melo, R. Prakash, N. Tejo Prakash

引用次数: 1

Continuous-flow biocatalysed kinetic resolution of 4-fluorophenyl-furan-2-yl methanol 连续流动生物催化动力学拆分4-氟苯-呋喃-2-基甲醇

IF 1.8 4区生物学

Biocatalysis and Biotransformation Pub Date : 2022-07-01 DOI: 10.1080/10242422.2022.2094258

R. D. de Souza, Raquel A. C. Leão, Barbara Maia, M. Gomez

{"title":"Continuous-flow biocatalysed kinetic resolution of 4-fluorophenyl-furan-2-yl methanol","authors":"R. D. de Souza, Raquel A. C. Leão, Barbara Maia, M. Gomez","doi":"10.1080/10242422.2022.2094258","DOIUrl":"https://doi.org/10.1080/10242422.2022.2094258","url":null,"abstract":"Abstract Enantiomerically pure secondary alcohols are useful in the synthesis of several natural products and as active pharmaceutical intermediates (API). Due to the high demand for these chiral compounds, much progress has been made in the areas of asymmetric synthesis and catalysis. In this context, biocatalysis together with continuous flow technology can be a valuable tool for more versatile and sustainable methods, with lower cost, greater stereoselectivity and less environmental impact. This work aims to obtain an enantiomerically pure alcohol of industrial interest, (4 Fluorophenyl) (furan-2-yl) methanol (3), by performing a kinetic resolution using immobilized Candida antarctica lipase B (Novozyme 435, N435) under continuous-flow conditions. Initial study was carried out to optimize batch reaction conditions. The best results were obtained using isooctane as solvent, 37.7 mg of N435 and three equivalents of isopropenyl acetate as acyl donor at 60 °C for 24 h. Under these conditions, a conversion of 49% and 91 of enantiomeric ratio was obtained. Optimized batch conditions were translated to the continuous flow reactor leading to the desired product in 30 min of residence time, 47% conversion and an enantiomeric ratio of 61.","PeriodicalId":8824,"journal":{"name":"Biocatalysis and Biotransformation","volume":"41 1","pages":"367 - 373"},"PeriodicalIF":1.8,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49092455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyanide bioremediation potential of Klebsiella oxytoca JCM 1665 rhodanese immobilized on alginate-glutaraldehyde beads 藻酸盐-戊二醛珠固定化羟基克雷伯菌JCM 1665硫丹酸盐的氰化物生物修复潜力

IF 1.8 4区生物学

Biocatalysis and Biotransformation Pub Date : 2022-06-17 DOI: 10.1080/10242422.2022.2087510

B. Itakorode, R. E. Okonji, N. Torimiro

{"title":"Cyanide bioremediation potential of Klebsiella oxytoca JCM 1665 rhodanese immobilized on alginate-glutaraldehyde beads","authors":"B. Itakorode, R. E. Okonji, N. Torimiro","doi":"10.1080/10242422.2022.2087510","DOIUrl":"https://doi.org/10.1080/10242422.2022.2087510","url":null,"abstract":"Abstract Rhodanese, a cyanide detoxifying enzyme synthesized by Klebsiella oxytoca was immobilized on alginate-glutaraldehyde beads. K. oxytoca was isolated from industrial wastewater and identified using 16S rRNA gene sequencing with gene bank accession number MN590525. Rhodanese was produced from the bacterium through submerged fermentation. The rhodanese produced was immobilized on alginate-glutaraldehyde beads; its physicochemical properties and cyanide bioremediation potential were compared with the free enzyme. In this study, the optimum concentrations of glutaraldehyde and sodium alginate that resulted in the highest enzyme immobilization yield (89.71%) and lower leakage (1.45 ± 0.2%) were 4.5% (v/v) and 2.5 (%) respectively. The optimum temperature for free and immobilized rhodanese was observed at 50 °C and 60 °C respectively while the optimum pH for both preparations was 6.0. The free rhodanese retained 31% and 23% of relative activity at 60 °C and 70 °C respectively after 30 minutes of incubation while immobilized rhodanese retained about 95% and 70% at the same condition. The entrapped rhodanese showed activity until the 10th cycle and maintained about 70% of its activity after the fifth cycle. After 180 minutes of incubation, the free and immobilized rhodanese was able to biodegrade 115 mg/L cyanide to 77 mg/L and 45 mg/L respectively with degradation efficiency of 33 and 64.34%. These results suggest that immobilized K. oxytoca rhodanese may be profitably exploited in bioremediation of cyanide polluted environment due to its thermal stability and its reusability.","PeriodicalId":8824,"journal":{"name":"Biocatalysis and Biotransformation","volume":"41 1","pages":"299 - 308"},"PeriodicalIF":1.8,"publicationDate":"2022-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49300559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Saccharification of pre-treated wheat straw via optimized enzymatic production using Aspergillus niger: Chemical analysis of lignocellulosic matrix 利用黑曲霉优化酶法生产预处理小麦秸秆的糖化:木质纤维素基质的化学分析

IF 1.8 4区生物学

Biocatalysis and Biotransformation Pub Date : 2022-06-15 DOI: 10.1080/10242422.2022.2087511

M. El-sheekh, Mohammed Y. Bedaiwy, Aya A. El-Nagar, Eman Elgammal

{"title":"Saccharification of pre-treated wheat straw via optimized enzymatic production using Aspergillus niger: Chemical analysis of lignocellulosic matrix","authors":"M. El-sheekh, Mohammed Y. Bedaiwy, Aya A. El-Nagar, Eman Elgammal","doi":"10.1080/10242422.2022.2087511","DOIUrl":"https://doi.org/10.1080/10242422.2022.2087511","url":null,"abstract":"Abstract Bioconversion of lignocellulosic wastes to valuable end-products via multi-enzymatic hydrolysis is a potential low costing process for large-scale application in many industrial sectors. Thus, in this study, among thirty local fungal isolates, Aspergillus niger which gave the highest cellulase production, was identified under the accession number MZ062603 in GeneBank. Three types of pre-treatments (0.0–0.7%) acid, (0.0–2%) alkali, and (70–90 °C) hot water were applied to increase wheat straw (WS) digestibility by A. niger, and 1% NaOH treated WS was superior to the other pre-treatments (acid and hot water). During solid-state fermentation, the total cellulolytic activity of [filter-paper cellulase (FPase), carboxy-methyl cellulase (CMCase), and β- glucosidase (βGase)] increased about 2.8-fold. While reducing sugar was increased by 3.1 times. The optimum values of total cellulases activities and reducing sugar (8907.2 and 92.4 mg/gds) were obtained after 3 days of incubation at 30 °C and pH 5.2 at 75% v/w moisture using 3 days old inoculum (106 spores/mL/gds). The WS substrate which was subjected to alkali pre-treatment subsequent to fungal bioconversion was varied in its chemical composition and detailed structure compared to the raw and alkali pre-treatment ones as indicated by its chemical analysis, Scanning electron microscopy (SEM) observation, Fourier Transform Infra-red Spectroscopy (FTIR), and X-Ray Diffraction (XRD). All these analyses revealed that the lignocellulosic matrix was completely destroyed after the fungal treatment.","PeriodicalId":8824,"journal":{"name":"Biocatalysis and Biotransformation","volume":"41 1","pages":"309 - 321"},"PeriodicalIF":1.8,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43144120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Biocatalytic synthesis of dioctyl sebacate in toluene using an immobilised lipase 固定化脂肪酶在甲苯中的生物催化合成癸二酸二辛酯

IF 1.8 4区生物学

Biocatalysis and Biotransformation Pub Date : 2022-06-15 DOI: 10.1080/10242422.2022.2087512

Mingxin Zhu, Shuyi Guo, Rongchen Mang, Hua Zhou

{"title":"Biocatalytic synthesis of dioctyl sebacate in toluene using an immobilised lipase","authors":"Mingxin Zhu, Shuyi Guo, Rongchen Mang, Hua Zhou","doi":"10.1080/10242422.2022.2087512","DOIUrl":"https://doi.org/10.1080/10242422.2022.2087512","url":null,"abstract":"Abstract Sebacic esters have excellent lubricity, thermal stability, and biodegradability and therefore are widely used as aerospace lubricants, metal working oil, or engine oil. They are mainly produced by chemical synthesis which, however, may cause environmental pollution, its enzymatic synthesis represents a more environmentally friendly alternative. A few reports have described the synthesis of sebacic esters employing immobilised lipases, but these biocatalytic reactions were exclusively carried out in solvent-free systems and thus, could have been limited by slow reaction rates and high reaction temperatures due to poor enzyme dispersion, low substrate solubility, and high viscosity of the reaction mixture. The current study investigated the biosynthesis of dioctyl sebacate in toluene by Novozym 435, a commercial immobilised lipase. The reaction parameters were investigated using the single factor approach and an orthogonal array design. The optimal conditions obtained were as follows: 10 mL toluene, sebacic acid,1 mmol (202.25 mg); molar ratio of sebacic acid to 1-octanol, 1:3; Novozym 435, 0.03 g; 4 Å molecular sieves, 1.5 g; reaction temperature, 40 °C; reaction time, 30 h. A dioctyl sebacate conversion rate of 93% was achieved under these optimal conditions. In particular, the addition of molecular sieves to the reaction mixture markedly improved the product yield. The reaction temperature was low enough to make the operation easy and energy-efficient and therefore, well suited for large-scale production.","PeriodicalId":8824,"journal":{"name":"Biocatalysis and Biotransformation","volume":"41 1","pages":"395 - 402"},"PeriodicalIF":1.8,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44827323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of the white rot fungus Trametes hirsuta 072 laccases ability to modify 17β-oestradiol in the aqueous medium 白腐菌毛毡菌072漆酶在水溶液中修饰17β-雌二醇能力的比较分析

IF 1.8 4区生物学

Biocatalysis and Biotransformation Pub Date : 2022-06-13 DOI: 10.1080/10242422.2022.2085034

O. Savinova, P. N. Solyev, T. Fedorova, S. Kochetkov, T. Savinova

引用次数: 0

Remediation of chlorpyrifos in soil using immobilized bacterial consortium biostimulated with organic amendment 固定化菌群对土壤毒死蜱的修复研究

IF 1.8 4区生物学

Biocatalysis and Biotransformation Pub Date : 2022-06-09 DOI: 10.1080/10242422.2022.2085033

Elizabeth Mary John, J. Sreekumar, M. S. Jisha

引用次数: 0