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Biocatalysis and Biotransformation最新文献

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Identification and characterization of a novel thermostable transaminase (TATP) from Thermorudis peleae peleae Thermorudis中一种新型热稳定转氨酶（TATP）的鉴定与表征
IF 1.8 4区 生物学
Biocatalysis and Biotransformation Pub Date : 2023-08-01 DOI: 10.1080/10242422.2023.2241601
Xiao Li, Ping Gui, Ruolin Yang, Zelin Lu, Xuefeng Wang, Chengkun Luo, Jingjie Jiang, Fuqiang Ma
{"title":"Identification and characterization of a novel thermostable transaminase (TATP) from Thermorudis peleae","authors":"Xiao Li, Ping Gui, Ruolin Yang, Zelin Lu, Xuefeng Wang, Chengkun Luo, Jingjie Jiang, Fuqiang Ma","doi":"10.1080/10242422.2023.2241601","DOIUrl":"https://doi.org/10.1080/10242422.2023.2241601","url":null,"abstract":"","PeriodicalId":8824,"journal":{"name":"Biocatalysis and Biotransformation","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42750457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Thermostable alkaline protease from Scytalidium thermophilum: production, purification, and biochemical characterization 嗜热镰刀菌耐热碱性蛋白酶:生产、纯化和生化表征
IF 1.8 4区 生物学
Biocatalysis and Biotransformation Pub Date : 2023-07-15 DOI: 10.1080/10242422.2023.2236760
Y. Yuzugullu Karakus, Gulen Sinem Inci, Elif Kale Bakir, Bektore Mansurov
{"title":"Thermostable alkaline protease from Scytalidium thermophilum: production, purification, and biochemical characterization","authors":"Y. Yuzugullu Karakus, Gulen Sinem Inci, Elif Kale Bakir, Bektore Mansurov","doi":"10.1080/10242422.2023.2236760","DOIUrl":"https://doi.org/10.1080/10242422.2023.2236760","url":null,"abstract":"Abstract An extracellular alkaline protease from Scytalidium thermophilum was produced in a glucose-containing medium supplemented with 5 mM NaCl for 3 days at pH 8.0 and 45 °C. The enzyme was 10-fold purified using ammonium sulfate precipitation followed by ion-exchange chromatography, and its molecular weight was calculated as 80 kDa from SDS-PAGE. The enzyme exhibited optimum activity at pH 8.0 and 60 °C. It was stable at pH and temperature range of 6.0–10.0 and 30–80 °C, respectively. Its half time was 30 h at pH 6.0, 7.0 and 8.0, while those were 22, 16, 8, and 3 h at 50 °C, 60 °C, 70 °C, and 80 °C, respectively. Kinetic parameters including Km (2 ± 0.02 mg/ml), Vmax (18.7 ± 1.5 µmole tyrosine ml−1 min−1), and kcat (2.5 x 103 min−1) were determined using casein. Ca2+ increased the enzyme activity, but it was slightly reduced by EDTA, Triton X-100, Tween 20, and Tween 80. It was active against reducing agents like β-mercaptoethanol but completely inhibited by phenyl methyl sulphonyl fluoride supporting the enzyme belonging to the serine protease family. Chloroform (143%), methanol (138%), and isopropanol (111%) increased the enzyme activity at 5% (v/v), while ethanol (71%) and acetone (81%) moderately reduced the proteolytic activity at the same concentration. Dimethyl sulfoxide (5%, v/v) did not significantly affect the enzyme. The enzyme was compatible with several detergents (1%, w/v), maintaining more than 90% of its original activity in almost all detergents tested. The stability of the enzyme presented against pH, temperature, organic solvents, and detergents indicates its potential use in various industrial applications, especially in peptide synthesis and the laundry industry.","PeriodicalId":8824,"journal":{"name":"Biocatalysis and Biotransformation","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46661763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A review on preparation and applications of glycerol carbonate: Focus biocatalysis 碳酸甘油酯的制备及应用综述：聚焦生物催化
IF 1.8 4区 生物学
Biocatalysis and Biotransformation Pub Date : 2023-07-06 DOI: 10.1080/10242422.2023.2232914
S. Karmee
{"title":"A review on preparation and applications of glycerol carbonate: Focus biocatalysis","authors":"S. Karmee","doi":"10.1080/10242422.2023.2232914","DOIUrl":"https://doi.org/10.1080/10242422.2023.2232914","url":null,"abstract":"","PeriodicalId":8824,"journal":{"name":"Biocatalysis and Biotransformation","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43245921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhanced biodegradation of crude oil contamination by indigenous bacterial consortium under real conditions 真实条件下原生菌群对原油污染的强化生物降解
IF 1.8 4区 生物学
Biocatalysis and Biotransformation Pub Date : 2023-07-06 DOI: 10.1080/10242422.2023.2231592
M. Baltaci, M. A. Omeroglu, H. Ozkan, Mesut Taskin, A. Adiguzel
{"title":"Enhanced biodegradation of crude oil contamination by indigenous bacterial consortium under real conditions","authors":"M. Baltaci, M. A. Omeroglu, H. Ozkan, Mesut Taskin, A. Adiguzel","doi":"10.1080/10242422.2023.2231592","DOIUrl":"https://doi.org/10.1080/10242422.2023.2231592","url":null,"abstract":"","PeriodicalId":8824,"journal":{"name":"Biocatalysis and Biotransformation","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47758433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Improving hydrogen peroxide stability of a dye-decolorising peroxidase from Irpex lacteus F17 by site-directed mutagenesis 通过定点诱变提高Irpex lacteus F17染料脱色过氧化物酶的过氧化氢稳定性
IF 1.8 4区 生物学
Biocatalysis and Biotransformation Pub Date : 2023-07-04 DOI: 10.1080/10242422.2023.2231122
Wen-Yi Huang, Jingjing He, Liuqing Li, Rong Jia
{"title":"Improving hydrogen peroxide stability of a dye-decolorising peroxidase from Irpex lacteus F17 by site-directed mutagenesis","authors":"Wen-Yi Huang, Jingjing He, Liuqing Li, Rong Jia","doi":"10.1080/10242422.2023.2231122","DOIUrl":"https://doi.org/10.1080/10242422.2023.2231122","url":null,"abstract":"","PeriodicalId":8824,"journal":{"name":"Biocatalysis and Biotransformation","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43187327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Tyrosinase from the peels of Musa cavendish: purification, characterization, immobilization and application in phenol biodegradation 麝香果皮酪氨酸酶的纯化、表征、固定化及其在苯酚生物降解中的应用
IF 1.8 4区 生物学
Biocatalysis and Biotransformation Pub Date : 2023-06-14 DOI: 10.1080/10242422.2023.2222206
M. Adeyanju, A. Ademakinwa
{"title":"Tyrosinase from the peels of Musa cavendish: purification, characterization, immobilization and application in phenol biodegradation","authors":"M. Adeyanju, A. Ademakinwa","doi":"10.1080/10242422.2023.2222206","DOIUrl":"https://doi.org/10.1080/10242422.2023.2222206","url":null,"abstract":"","PeriodicalId":8824,"journal":{"name":"Biocatalysis and Biotransformation","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44654707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Chitinase: a potent biocatalyst and its diverse applications 几丁质酶：一种有效的生物催化剂及其应用
IF 1.8 4区 生物学
Biocatalysis and Biotransformation Pub Date : 2023-06-02 DOI: 10.1080/10242422.2023.2218524
Gaytri Mahajan, Vibhuti Sharma, Reena Gupta
{"title":"Chitinase: a potent biocatalyst and its diverse applications","authors":"Gaytri Mahajan, Vibhuti Sharma, Reena Gupta","doi":"10.1080/10242422.2023.2218524","DOIUrl":"https://doi.org/10.1080/10242422.2023.2218524","url":null,"abstract":"","PeriodicalId":8824,"journal":{"name":"Biocatalysis and Biotransformation","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48379316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Modification of the properties of the metagenomic lipase LipC12 by engineering of the hydrophobic cavity 疏水腔工程修饰宏基因组脂肪酶LipC12的性质
IF 1.8 4区 生物学
Biocatalysis and Biotransformation Pub Date : 2023-05-05 DOI: 10.1080/10242422.2023.2202803
Marcos Rodrigues Maldonado, R. Alnoch, Gabriela Yumi Yamamoto Shiratori, C. C. de Oliveira, Marcos Brown Gonçalves, D. Mitchell, N. Krieger
{"title":"Modification of the properties of the metagenomic lipase LipC12 by engineering of the hydrophobic cavity","authors":"Marcos Rodrigues Maldonado, R. Alnoch, Gabriela Yumi Yamamoto Shiratori, C. C. de Oliveira, Marcos Brown Gonçalves, D. Mitchell, N. Krieger","doi":"10.1080/10242422.2023.2202803","DOIUrl":"https://doi.org/10.1080/10242422.2023.2202803","url":null,"abstract":"","PeriodicalId":8824,"journal":{"name":"Biocatalysis and Biotransformation","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41597723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Increased trypsin resilience in aqueous-acetonitrile environment when immobilized on glyoxyl-agarose may improve its applicability 当固定化在乙二酰琼脂糖上时，胰蛋白酶在乙腈水溶液环境中的弹性增加，可能会提高其适用性
IF 1.8 4区 生物学
Biocatalysis and Biotransformation Pub Date : 2023-04-19 DOI: 10.1080/10242422.2023.2202804
J. P. Miguez, Ana Elisa Almeida Sousa, O. Tavano
{"title":"Increased trypsin resilience in aqueous-acetonitrile environment when immobilized on glyoxyl-agarose may improve its applicability","authors":"J. P. Miguez, Ana Elisa Almeida Sousa, O. Tavano","doi":"10.1080/10242422.2023.2202804","DOIUrl":"https://doi.org/10.1080/10242422.2023.2202804","url":null,"abstract":"","PeriodicalId":8824,"journal":{"name":"Biocatalysis and Biotransformation","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43903705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Recent developments in biohydrogen production from wastewater: A review 废水生物制氢研究进展
IF 1.8 4区 生物学
Biocatalysis and Biotransformation Pub Date : 2023-04-10 DOI: 10.1080/10242422.2023.2181046
C. Ram, P. Rani, Amit Kumar
{"title":"Recent developments in biohydrogen production from wastewater: A review","authors":"C. Ram, P. Rani, Amit Kumar","doi":"10.1080/10242422.2023.2181046","DOIUrl":"https://doi.org/10.1080/10242422.2023.2181046","url":null,"abstract":"","PeriodicalId":8824,"journal":{"name":"Biocatalysis and Biotransformation","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44081761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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