BIO Web of Conferences最新文献

{"title":"Specificity of the effect of different endophytic Bacillus velezensis strains on soybean cultivars with contrasting growth habits","authors":"Radik I. Safin, Valeria A. Kolesar, D.R. Safina, Gulnaz Kh. Shaimullina","doi":"10.1051/bioconf/202623801038","DOIUrl":"https://doi.org/10.1051/bioconf/202623801038","url":null,"abstract":"This study evaluates the influence of the source of isolation of an endophytic seed-borne isolate within a single bacterial species on its subsequent compatibility with a new host. Two Bacillus velezensis strains were tested: one (KS 38 AU) isolated from barley seeds and the other (KS 63 AU) from soybean seeds. Seed treatments based on these strains were applied to soybean (Glycine max L.). Two soybean cultivars differing in growth habit served as experimental material: cv. Lidiya (indeterminate type) and cv. Apis (determinate type). Plants were grown in a climate chamber under controlled conditions. At the V3 growth stage, leaf photosynthetic pigment content and free proline accumulation were assessed. Strain KS 63 AU was compatible with both soybean cultivars, increasing chlorophyll *a* content and reducing proline levels. In contrast, in the determinate cv. Apis, strain KS 38 AU caused a sharp decline in chlorophyll *a* content (to 36% of the control), yet paradoxically reduced proline to levels comparable with those of the compatible strain. The Cultivar × Strain interaction for proline was highly significant (p = 0.004). The results obtained for the determinate cv. Apis indicate that strain KS 38 AU may trigger accelerated leaf senescence with concomitant nitrogen remobilization. These findings demonstrate that endophyte origin and plant growth habit jointly determine the outcome of inoculation, which has direct implications for the rational selection of microbial inoculants based on seed-borne endophytic bacteria in legume production.","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":"238 1","pages":"01038-01038"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.bio-conferences.org/articles/bioconf/pdf/2026/31/bioconf_esdca2026_01038.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148305545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of Different Molecular Weight Chitosan Dip-coating on the Quality of <i>Monopterus albus</i> during Cold Storage","authors":"Shixin Sun, Qiufeng Huang, Yanrong Song, Hao Zhang, Li Zhao, chunqing Bai","doi":"10.1051/bioconf/202622201010","DOIUrl":"https://doi.org/10.1051/bioconf/202622201010","url":null,"abstract":"In this research, the impacts of chitosan with different molecular weights (30, 150, 300 kDa) on the quality of Monopterus albus during refrigeration storage were investigated in terms of total colony count (TCC), ph, TVB-N , water loss rate, and cooking loss rate. The results indicated that the 150 kDa chitosan dip-coating demonstrated superior performance in extending the shelf life of Monopterus albus by preserving tissue structure, reducing cellular damage, and moisture loss. This research would supply new insights into widening chitosan as natural preservative in aquatic product preservation.","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":"222 1","pages":"01010-01010"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.bio-conferences.org/articles/bioconf/pdf/2026/15/bioconf_icare2026_01010.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147382037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction and In Vitro Validation of Liposomes Loaded with ROS-Responsive Probe QM-CF","authors":"Chen Cheng, Mingxi Li, Fang Yang","doi":"10.1051/bioconf/202623702020","DOIUrl":"https://doi.org/10.1051/bioconf/202623702020","url":null,"abstract":"In this study, liposomes loaded with the reactive oxygen species-responsive probe QM-CF (Lipo@QM-CF) were prepared using a phospholipid composition consisting of dipalmitoylphosphatidylcholine (DPPC), sphingomyelin (SM), cholesterol (CHO), DSPE-PEG2000, and DSPE-PEG2000-maleimide, selected for their roles in membrane stability, fluidity, and potential for functional modification. The particle size, stability, encapsulation efficiency, and probe loading status of the liposomes were preliminarily investigated. The results showed that the prepared liposomes exhibited uniform particle size (approximately 160–170 nm, PdI &lt; 0.15) and showed no significant changes in particle size during 21 days of storage at 4°C, indicating good in vitro stability. Elemental analysis revealed that chlorine signals colocalized with the liposome positions, suggesting the colocalization of QM-CF with liposomes. Chemiluminescence imaging further verified the probe loading status: free QM-CF exhibited luminescence only in methanol, while Lipo@QM-CF showed no luminescence but recovered upon methanol demulsification, with further enhancement in the presence of reactive oxygen species, confirming that QM- CF was successfully encapsulated within liposomes and possessed good ROS-responsive release capability. This study successfully demonstrates the construction of a liposome-based probe for ROS imaging, offering a novel strategy for detecting inflammatory microenvironments.","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":"237 1","pages":"02020-02020"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.bio-conferences.org/articles/bioconf/pdf/2026/30/bioconf_icbb2026_02020.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148408664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Luciferase-Based Co-Culture System for Convenient Monitoring of CD8⁺ T Cell Cytotoxicity in Tumor Immunotherapy","authors":"Zhixin Dong, J. Li, Hai‐Long Wu","doi":"10.1051/bioconf/202518202007","DOIUrl":"https://doi.org/10.1051/bioconf/202518202007","url":null,"abstract":"Current methods for assessing CD8 + T cell cytotoxicity in tumor immunotherapy, such as Annexin V/PI staining or lactate dehydrogenase assays, often involve laborious protocols requiring complex workflows. Here, we developed a luciferase-based co-culture system that enables rapid and convenient evaluation of CD8 + T cell-mediated tumor killing through direct supernatant analysis. CD8 + T cells (&gt;85% purity) were isolated from OT-1 mouse spleens and activated with SIINFEKL peptide and IL-2. B16F10 melanoma cells were stably transfected with firefly luciferase (B16F10-FLuc) to allow monitoring of tumor cell viability. In co-culture assays, luminescence signals from supernatants peaked at 3–5 hours and correlated strongly with T cell cytotoxicity. Pretreatment of tumor cells with Atractylenolide I (ATT-I), an MHC-I enhancer, amplified luminescence, validating the system’s sensitivity to immune modulation. This method eliminates cell lysis and centrifugation steps while maintaining high reproducibility. Our platform offers a scalable tool for screening immunotherapeutic agents (e.g., checkpoint inhibitors) and dissecting T cell-tumor interaction mechanisms.","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":"182 1","pages":"02007-02007"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.bio-conferences.org/articles/bioconf/pdf/2025/33/bioconf_icfsb2025_02007.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147901223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of macromicroelement complexes on productivity and photosynthetic activity of anthocyanin-containing potato varieties","authors":"Maksim Pavlov, Polina V. Kulagina, Karina N. Khomyakova","doi":"10.1051/bioconf/202517908003","DOIUrl":"https://doi.org/10.1051/bioconf/202517908003","url":null,"abstract":"In the conditions of the Central Non-Chernozem region of Russia, the productivity and photosynthetic activity of anthocyanin- containing potato varieties Surpriz, Severnoye siyanie. and Amethyst were studied for various applications of macromicroelement complexes: the formulation of 2020 against the background of N 120 P 140 K 180 and calculated on the basis of indicators of the removal of nutrients for yields of 30 and 40 t/ha (100% before planting, 99.5% before planting + 0.5% in foliar top dressing; 99% before planting + 1 % in foliar top dressing). The highest yield of tubers (36.4 t/ha) was obtained from the Surpriz variety, which had a higher yield than the Severnoye siyanie and Amethyst varieties by 4.8-5.9 t/ha, which ensures a higher net photosynthetic productivity (NPP) compared to other varieties by 0.9-1.1 g/m2 day. For all varieties, the most effective was the use of MMC 2020 against the NPP background, which provided an increase in different varieties from 9.0 to 14.4 t/ha due to the growth of the leaf surface (leaf area and PP), as well as the content of photosynthetic pigments in the leaves (CCI index). MMC can also be selected as optimal for all varieties in a dose calculated for approximately 30 t/ha of tubers in a variant combining 99.5% of the weight of the elements before planting and 0.5% in non-root top dressing. At the same time, all varieties have achieved the planned yield level, and the Severnoye siyanie variety has the maximum increase in control (7.6 t/ha), which is ensured by an increase in the NPP by 0.47-1.50 g/m 2 per day and the CCI index during the maximum period for Surpriz and Severnoye siyanie varieties by 1.67-1.73 units.","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":"179 1","pages":"08003-08003"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.bio-conferences.org/articles/bioconf/pdf/2025/30/bioconf_idsisa2025_08003.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147910154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating The Impact Of Homoharringtonine On K562 Cell Viability And ER Stress Pathways","authors":"Meishi Wang, Sensen Zhao, Shuangle Yu, Zhaoli Zhou","doi":"10.1051/bioconf/202516303002","DOIUrl":"https://doi.org/10.1051/bioconf/202516303002","url":null,"abstract":"This study investigated the effects of Homoharringtonine (HHT) on K562 cell proliferation and endoplasmic reticulum (ER) stress. The inhibitory effect of HHT was assessed using the CCK-8 assay to calculate IC50 values. Flow cytometry evaluated cell cycle distribution post-HHT exposure, while Proteostat dye assessed protein aggregation. Expression levels of XBP1s and related markers (BIP, CHOP, IRE1α) were measured to analyze ER stress. Results indicated that HHT significantly reduced K562 cell viability, yielding an IC50 value of 28.53 nM. HHT treatment caused cell accumulation in the G0/G1 phase, indicating cell cycle arrest. It also activated ER stress pathways, leading to increased levels of XBP1s, BIP, and CHOP. The combination of HHT with the ER stress inhibitor 4PBA alleviated HHT-induced ER stress, enhancing its anti-tumor effects. This study demonstrates that HHT inhibits K562 cell proliferation while activating ER stress pathways, suggesting that modulating ER stress may enhance its therapeutic efficacy in myeloid leukemia. Further research is required to elucidate the underlying mechanisms.","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":"163 1","pages":"03002-03002"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.bio-conferences.org/articles/bioconf/pdf/2025/14/bioconf_icbbb2025_03002.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147898350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The chemical composition of the leaves of the “white turnip” and “yellow turnip” varieties of turnip","authors":"Nargiza Baxtiyorjon qizi Atakulova","doi":"10.1051/bioconf/202519401040","DOIUrl":"https://doi.org/10.1051/bioconf/202519401040","url":null,"abstract":"This study quantitatively compares the vitamin content in leaves of Brassica rapa L. (turnip) cultivars ‘White turnip’ and ‘Yellow turnip’ using the Yussch analytical method. The investigation focuses on water- soluble vitamin composition and its nutritional implications. As a result of the cited data, the fact that turnip has a rich vitamin composition, which is important in the Prevention of certain diseases and strengthening the immune system of the human body, has been widely covered on the basis of scientific sources. Analytical results demonstrate that 100 g of white turnip leaves contain: vitamins B 1 67.3 mg, B 3 15.5 mg, PP 21.16 mg, B 9 10.7 mg, C 2.36 mg, and B 2 4.36 mg. The sequence of decreasing vitamins is as follows: B 1 &gt; PP&gt; B 3 &gt; B 9 &gt; B 2 &gt; C&gt; B 6 &gt; B 12 . Our next sample is B 3 61.1 mg, PP 12.9 mg, B 9 11.54 mg, B 2 14 mg, and C 10.5 mg in the 100 g yellow turnip leaf section. In this case, we can see vitamins B 3 &gt;B 2 &gt;PP&gt;B 9 &gt;C&gt;B 1 &gt;B 6 &gt;B 12 in the order of decreasing.","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":"194 1","pages":"01040-01040"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.bio-conferences.org/articles/bioconf/pdf/2025/45/bioconf_bft2025_01040.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147903868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Ten Types of Diets on Nitrogen Balance and Excretion of Purine Derivatives in Hu Sheep","authors":"Lihong Hu, Huey‐Jen Su, Zuhui Pu, Shihong Yang, Yin Ma, Halidai Rehemujiang, Guishan Xu","doi":"10.1051/bioconf/202518201014","DOIUrl":"https://doi.org/10.1051/bioconf/202518201014","url":null,"abstract":"This experiment aimed to investigate the effects of ten concentrate feedstuffs on nitrogen balance and urinary purine derivative (PD) excretion in Hu sheep. Fifteen healthy 5-month-old Hu sheep (average body weight:25.31±2.24 kg) were randomly divided into 3 groups (5 sheep per group). The experiment consisted of 4 phases, with 3 treatments per phase and 5 replicates per treatment. A single-factor design was adopted, and the experimental diets comprised 10 total mixed rations (TMR). Each diet was fed for 20 days, including a 15-day adaptation period followed by a 5-day formal trial period, during which total feces and urine were collected and stored at −20°C. Results showed that nitrogen intake and total nitrogen excretion in sheep fed soybean meal, rapeseed meal, walnut meal, flaxseed meal, cottonseed meal, and rice distillers’ grains diets were significantly higher than those fed oat-,wheat-, corn-, and wheat bran-based diets ( P &lt;0.05). Urinary nitrogen excretion in the walnut meal and soybean meal groups was significantly higher than in the other eight diets ( P &lt;0.05). Absorbed nitrogen and nitrogen retention in the soybean meal and rapeseed meal groups were significantly higher than in the other eight diets ( P &lt;0.05). Urinary uric acid excretion ranged from 0.14 to 0.40 mmol/d,with allantoin accounting for 85.63%–89.26% of total PD excretion. The proportion of uric acid in total PD excretion (0.81%–2.14%) was significantly lower in the rapeseed meal diet than in the other nine diets ( P &lt; 0.05). Xanthine and hypoxanthine collectively represented 9.73%–12.92% of total PD excretion. The purine nitrogen index (PNI) of the four energy-supplemented diets was significantly higher than that of the five protein-supplemented diets (excluding rice distillers’ grains) ( P &lt;0.05). These findings indicate that dietary composition and concentrate type significantly influence nitrogen excretion patterns in Hu sheep. Under the experimental conditions,sheep fed low-protein diets excreted nitrogen via both fecal and urinary pathways,whereas high-protein diets shifted nitrogen excretion predominantly to urine,with urinary nitrogen surpassing fecal nitrogen. Urinary PD excretion increased proportionally with elevated dietary nitrogen levels. This study provides critical insights into optimizing feed formulations to enhance nitrogen utilization efficiency and mitigate environmental pollution in ruminant production systems.","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":"182 1","pages":"01014-01014"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.bio-conferences.org/articles/bioconf/pdf/2025/33/bioconf_icfsb2025_01014.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147331104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing the biocontrol ability of Bacillus against spoilage fungus with screened booster for postharvest fruit preservation","authors":"Tengyu Cui, Xin Luo","doi":"10.1051/bioconf/202517402018","DOIUrl":"https://doi.org/10.1051/bioconf/202517402018","url":null,"abstract":"A broad spectrum of studies is undertaken in postharvest fruit preservation. This particular research was focused on the combined application of Bacillus amyloliquefaciens (B. amyloliquefaciens) and eugenol solution to inhibit the growth of Penicillium expansum (P. expansum) mycelia. The research encompassed tests on P. expansum spore germination rate, membrane permeability of P. expansum mycelia cells, decay rate determination of grapes and blueberries, measurement of total soluble solids (TSS), and evaluation of Glutathione Peroxidase (GSH-Px) enzyme activity. The findings revealed that the eugenol solution exhibited superior performance in inducing nucleic acid and protein leakage. Consequently, the research opted for the eugenol solution as a synergistic agent with B. amyloliquefaciens for postharvest fruit preservation. Grapes displayed more pronounced inhibition effects compared to blueberries. Subsequent investigations demonstrated that the combined approach of eugenol solution and B. amyloliquefaciens exhibited greater inhibitory effects than individual applications or the control group. Consequently, postharvest fruits can be effectively preserved, and the mycelial growth of pathogenic fungi can be restrained by using B. amyloliquefaciens and eugenol solution as a viable biocontrol technique. Moreover, several considerations must be addressed before adopting this method as an established biocontrol strategy. These considerations encompass the stability and availability of eugenol solution at elevated temperatures, enhanced inhibition against other types of pathogenic fungi, and identification of optimal storage conditions for biocontrol. However, to validate the safety of the approach, further studies are necessary to investigate any adverse effects associated with using boosters and B. amyloliquefaciens.","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":"174 1","pages":"02018-02018"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.bio-conferences.org/articles/bioconf/pdf/2025/25/bioconf_icbb2025_02018.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147886255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utilization of <i>Papaya</i> Waste <i>(Carica papaya</i> L.) as Biopesticide to Manage the Aphid Population in Chili ( <i>Capsicum annuum</i> L.)","authors":"Dian Indratmi, Dyah Roeswitawati, Iswandy Iswandy, Hidayat Widi Saputra","doi":"10.1051/bioconf/202520102013","DOIUrl":"https://doi.org/10.1051/bioconf/202520102013","url":null,"abstract":"Chili cultivation (Capsicum annuum L.) is a farming activity that promises attractive profits due to high market demand. The low production of horticultural crops, among others, can be caused by plant disrupting organisms in the form of pests, diseases and weeds. The use of chemicals is an alternative for farmers to inhibit pest and disease attacks. Aphid attack causes plants to grow abnormally, with leaves turning yellow, crinkling, and dying. The importance of developing long-term pesticides for environmental health is crucial, so that the use of natural pesticides derived from papaya leaves and seeds can be considered. The purpose of the study was to determine the right concentration and effectiveness of papaya leaf and seed waste in suppressing aphid pests. The study used an experimental method with various concentrations arranged in a Randomized Group Design. The results of the evaluation of the application leaves+ papaya seeds showed a better concentration treatment for the variables of pest population, intensity of attack, percentage of infested leaves, and yield of chili 90-120 g/l. With the application of leaves, papaya seeds can increase chili production 2.5 times.","PeriodicalId":8805,"journal":{"name":"BIO Web of Conferences","volume":"201 1","pages":"02013-02013"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.bio-conferences.org/articles/bioconf/pdf/2025/52/bioconf_icon-beat2025_02013.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147915478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}