Biodiversitas Journal of Biological Diversity最新文献

{"title":"Species richness and dispersion patterns of Lepidoptera (Rhopalocera) in the Nuraksa Forest Park, Lombok, Indonesia","authors":"M. L. Ilhamdi, Agil Al Idrus, D. Santoso, G. Hadiprayitno, M. Syazali, Ismawan Hariadi","doi":"10.13057/biodiv/d250108","DOIUrl":"https://doi.org/10.13057/biodiv/d250108","url":null,"abstract":"Abstract. Ilhamdi ML, Idrus AA, Santoso D, Hadiprayitno G, Syazali M, Hariadi I. 2023. Species richness and dispersion patterns of Lepidoptera (Rhopalocera) in the Nuraksa Forest Park, Lombok, Indonesia. Biodiversitas 25: 62-70. Butterflies play an important role in maintaining the balance of ecosystems and pollinators. This study aims to analyze the species richness and dispersion patterns of butterflies from the Rhopalocera suborder in this area. The field survey was carried out from January to July 2023. The sampling site was divided into six areas. Specimens that were successfully collected using a hand net were identified at the Biological Laboratory of Mataram University. During this survey, 1576 specimens of Rhopalocera were recorded. This number consists of 47 species. Several species, such as Junonia atlites Linnaeus, 1763, Junonia almana Linnaeus, 1758, Danaus genutia Cramer, 1779, Hypolimnas misippus Linnaeus, 1764, and Amathusia phidippus Linnaeus, 1763 were found to be new records in the Nuraksa Forest Park area. The habitat types with the highest number of species are the utilization block and the traditional block, with 47 species. The habitat type with the lowest number of species is a special block with only 15 species. However, the highest species richness was found in the protection block, with an R-value of 8.09. The most dominant species is Leptosia nina Fabricius, 1793, and the least dominant is A. phidippus. Each species depicted a varying distribution in the six habitat types. According to the analysis results, it was found that the dispersion pattern of all Rhopalocera species is clustered. The research results themselves contribute to conservation and ecotourism efforts.","PeriodicalId":8801,"journal":{"name":"Biodiversitas Journal of Biological Diversity","volume":"86 16","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140462487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-throughput analysis using 16S rRNA sequencing of bacterial communities associated in selected mangrove species from Bayug Island, Iligan City, Philippines","authors":"S. Siblos, S. Tabugo","doi":"10.13057/biodiv/d250107","DOIUrl":"https://doi.org/10.13057/biodiv/d250107","url":null,"abstract":"Abstract. Siblos SKV, Tabugo SR. 2023. High-throughput analysis using 16S rRNA sequencing of bacterial communities associated in selected mangrove species from Bayug Island, Iligan City, Philippines. Biodiversitas 25: 53-61. Mangrove ecosystems are recognized globally as highly productive ecosystems, that play a crucial role in carbon sequestration, erosion control, water purification, and as essential breeding grounds for diverse aquatic life. They also offer specialized ecological niches, accommodating a diverse range of organisms, including bacterial communities. This study aimed to investigate bacterial communities of Bayug Island in Iligan City, Philippines, inhabiting six mangrove species, namely Rhizophora stylosa Griffith, Rhizophora apiculata Blume, Rhizophora mucronata Lam., Sonneratia alba Sm., Ceriops tagal (Perr.) C.B.Rob., and Nypa fruticans Wurmb and their possible functions within the mangrove forests. Genomic DNA was extracted from the six pooled soil samples, and the V3-V4 region of the 16S rRNA gene was sequenced using the Illumina MiSeq platform. Six amplicon libraries, corresponding to the mangrove species were analyzed with Parallel Meta Suite software, yielding 173,270 amplicon sequence variants (ASVs) after quality control. The study identified the top five most abundant ASVs in the mangrove rhizosphere, linked to the genera Vibrio, Stenotrophomonas, Serratia, Pseudoalteromonas, and Achromobacter. Among the mangrove species, S. alba exhibited higher alpha diversity according to the Shannon index. PICRUSt analysis revealed that microorganisms are involved in biodegradation, xenobiotic metabolism, and other metabolic processes within the mangroves. These bacteria could find applications in environmental cleanup, bioremediation, waste treatment, and soil health improvement. This result contributes to the ongoing ecological restoration of mangrove forests at the research site.","PeriodicalId":8801,"journal":{"name":"Biodiversitas Journal of Biological Diversity","volume":"82 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140462515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population study of long-tailed macaque (Macaca fascicularis) on Deli Island, Banten, Indonesia","authors":"Yuli S. Fitriana, Eko Sulistyadi, Rizki Kurnia Tohir, Mhd Muhajir Hasibuan, Farid Rifaie, Ibnu Maryanto, Arie Mulia Lubis, M. F. Rifqi","doi":"10.13057/biodiv/d250114","DOIUrl":"https://doi.org/10.13057/biodiv/d250114","url":null,"abstract":"Abstract. Fitriana YS, Sulistyadi E, Tohir RK, Hasibuan MM, Rifaie F, Maryanto I, Lubis AM, Rifqi MF. 2024. Population study of long-tailed macaque (Macaca fascicularis) on Deli Island, Banten, Indonesia. Biodiversitas 25: 144-150. Understanding the distribution and population dynamics of primate species is essential for practical conservation efforts. However, such information is often unavailable, even for species whose populations appear abundant. The long-tailed macaque (Macaca fascicularis Raffles, 1821) is a species with a wide range in Southeast Asia, including Sumatra, Java, Kalimantan, Bali, Lombok, and Lesser Sunda in Indonesia. However, comprehensive data on population density across its Indonesian distribution area still needs to be improved. To address this knowledge gap, we conducted a thorough survey and study to estimate the population density of long-tailed macaques on Deli Island, located in Banten Province. Long-tailed macaques were introduced to Deli Island between 1989 and 1990 with a total of 1000 males and 10,000 females. To date, a total of 9,500 individuals have been harvested from this island in the period 1991-2010. We employed the line transect distance sampling (LTDS) method with a perpendicular distance approach that covered all habitat types throughout the island. Our findings revealed that the population density of long-tailed macaques on Deli Island is approximately five individuals per hectare, with an estimated total population of 4,860. The data pertaining to age structure and sex ratio is notably limited, primarily due to challenges in identification caused by dense vegetation. To address these limitations and acquire a more comprehensive understanding, additional research is essential for the accurate determination of the age structure and sex ratio on the island. Our study provides crucial baseline data for conserving this species. We recommend implementing a systematic survey encompassing the entire distribution range of the long-tailed macaque across Indonesia. Such an initiative will be instrumental in assessing its threat status and laying out a comprehensive plan for conservation efforts.","PeriodicalId":8801,"journal":{"name":"Biodiversitas Journal of Biological Diversity","volume":"208 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140462456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity of universal stress protein in Enterobacteriales and its reduced expressions on Pectobacterium brasiliense after manuka honey treatment","authors":"Chotimah Chotimah, Alan Soffan, Tri Joko","doi":"10.13057/biodiv/d250106","DOIUrl":"https://doi.org/10.13057/biodiv/d250106","url":null,"abstract":"Abstract. Chotimah, Soffan A, Joko T. 2024. Diversity of universal stress protein in Enterobacteriales and its reduced expressions on Pectobacterium brasiliense after manuka honey treatment. Biodiversitas 25: 49-52. Universal stress proteins (Usps) support the survival of an organism by increasing their expressions under stress conditions, which include nutritional deficiencies, heat shock, and antibiotics. However, the expression of uspA in Staphylococcus aureus decreased after manuka honey exposure. Manuka honey also inhibits the growth of Pectobacterium brasiliense, although its effect on the usp gene of this soft-rot pathogen has never been studied. This study aimed to determine the diversity of Usps in P. brasiliense and their orthologs from Enterobacteriales using phylogenetic analysis. The effects of manuka honey on usp gene expressions were also investigated using quantitative real-time polymerase chain reaction and pathogenicity assay of P. brasiliense on Chinese cabbage. The results revealed that the UspA, UspB, and UspE of P. brasiliense had the closest similarity to those of P. carotovorum. By contrast, UspG had the closest similarity to P. polaris. The usp gene expressions were downregulated by 5, 16, 10, and 62% in 5% (w/v) manuka honey treatment. When tested on Chinese cabbage, P. brasiliense treated with manuka honey caused smaller lesion symptoms than those in the control treatment. This reduced virulence of P. brasiliense may be related to the reduced expression of usp genes triggered by manuka honey.","PeriodicalId":8801,"journal":{"name":"Biodiversitas Journal of Biological Diversity","volume":"119 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140462220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parasitoid of coffee berry borer, Hypothenemus hampei Ferrari (Coleoptera: Curculionidae) at Bogor District, West Java, Indonesia","authors":"Hiksa Maulana Saputra, N. Maryana, Pudjianto Pudjianto","doi":"10.13057/biodiv/d241206","DOIUrl":"https://doi.org/10.13057/biodiv/d241206","url":null,"abstract":"Abstract. Saputra HM, Maryana N, Pudjianto. 2023. Parasitoid of coffee berry borer, Hypothenemus hampei Ferrari (Coleoptera: Curculionidae) at Bogor District, West Java, Indonesia. Biodiversitas 24: 6447-6453. The coffee berry borer (CBB), Hypothenemus hampei Ferrari (Coleoptera: Curculionidae), is an essential pest of coffee plants that can cause loss and decrease in the quality of coffee berries. Parasitoids were expected to affect the presence of coffee berry borer in the ecosystem as one of its natural enemies. This study aimed to identify and describe the parasitoid of CBB in Bogor District, West Java, Indonesia. The study was conducted from June 2022 to January 2023 on three coffee cultivation located in three sub-districts in Bogor District, i.e., Cijeruk, Dramaga, and Sukamakmur. This study collected attacked coffee berries by CBB from coffee cultivation that kept the coffee berry until the parasitoids emerged and the parasitoids were identified; the morphological characters and descriptions of parasitoids CBB were presented. Ten species of Hymenopteran parasitoids belonging to five families were found. These parasitoids were Microgastrinae, Mendesellinae (Braconidae), Trissolcus sp. (Scelionidae), Anagyrus sp., Marxella sp., Procheiloneurus sp., Pararhopella sp., Parablastothrix sp. (Encyrtidae), Pronotalia sp. (Eulophidae), and Pteromalus sp. (Pteromalidae). The parasitoids of CBB in Bogor District were quite diverse, but their abundance was low.","PeriodicalId":8801,"journal":{"name":"Biodiversitas Journal of Biological Diversity","volume":"45 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140490663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection of iron gene on multidrug resistant avian fecal Escherichia coli isolated from broiler on traditional markets, Surabaya, Indonesia","authors":"Mariana Febrilianti Resilinda Putri, Irfan Alias Kendek, F. J. Wibisono, M. Effendi, Dadik Rahardjo, W. Tyasningsih, E. N. Ugbo","doi":"10.13057/biodiv/d241207","DOIUrl":"https://doi.org/10.13057/biodiv/d241207","url":null,"abstract":"Abstract. Putri MFR, Kendek IA, Wibisono FJ, Effendi MH, Rahardjo D, Tyasningsih W, Ugbo EN. 2023. Molecular detection of iron gene on multidrug resistant avian fecal Escherichia coli isolated from broiler on traditional markets, Surabaya, Indonesia. Biodiversitas 24: 6454-6460. Avian Fecal Escherichia coli (AFEC) is the cause of colibacillosis, which often infects the poultry industry throughout the world. The virulence gene influences the iroN factor, which causes systemic infections in poultry. This research aimed to determine Multidrug Resistance (MDR) in AFEC obtained from traditional markets in Surabaya. There were 96 cloacal swab samples from broiler chickens. The samples were isolated and identified using culture media in the form of Eosin Methylene Blue Agar (EMBA), gram stain, Triple Sugar Iron Agar (TSIA), Sulfide Indole Motility (SIM), Simmons Citrate Agar (SCA), Voges Proskauer (MR-VP) indole and methyl test; detection of multidrug resistance using Mueller-Hinton Agar (MHA) media. The results of the isolation and identification of Escherichia coli bacteria in this study showed that 60.4% of the isolates were positive for Escherichia coli. Escherichia coli is resistant to tetracycline antibiotics by 56%, ciprofloxacin by 55%, antibiotics aztreonam 29%, kanamycin 20%, and chloramphenicol 18%. The multidrug resistance test result on Escherichia coli was 25.8%. The PCR test results for the iroN gene were 40%. Therefore, there are MDR and iroN genes in avian fecal Escherichia coli in Surabaya traditional markets; APEC with iroN gene poses the potential to affect public health.","PeriodicalId":8801,"journal":{"name":"Biodiversitas Journal of Biological Diversity","volume":"175 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140501352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Barcoding of ornamental freshwater shrimp, Neocaridina denticulata (De Haan, 1844) from the aquatic ornamental market in Purbalingga, Central Java, Indonesia","authors":"E. S. Kusmintarsih, Hanan Hassan Alsheikh Mahmoud, M. H. Sastranegara, Hamdan Syakuri, Agus Nuryanto, T. B. Ambarningrum","doi":"10.13057/biodiv/d240714","DOIUrl":"https://doi.org/10.13057/biodiv/d240714","url":null,"abstract":"Abstract. Kusmintarsih ES, Mahmoud HHA, Sastranegara MH, Syakuri H,Nuryanto A, Ambarningrum TB. 2023. Barcoding of ornamental freshwater shrimp, Neocaridina denticulata (De Haan, 1844) from the aquatic ornamental market in Purbalingga, Central Java, Indonesia. Biodiversitas 24: 3601-3608. Indonesia is one of the world’s leading aquatic ornamental shrimps producers and exports. One of the aquatic ornament shrimp species is a Neocaridina denticulate (De Haan, 1844). There is a wide range of colors including red, bright red, yellow, orange, green, blue, violet, black, and color combinations. It is a landlocked Atydiae family species comprising31 species and subspecies. Due to taxonomic difficulties, this species is continually under revision, and the validation of a few species is currently questionable and uncertain for the relationships among the various colors of N. denticulata. The Cytochrome Oxidase gene I (CO1) gene sequences of mitochondrial DNA were used for species DNA barcoding studies. This study aimed to validate the taxonomic status of different colors of N. denticulata species using the mitochondrial DNA or mtDNA Cytochrome oxidase (CO1) gene sequences. Neighbour Joining trees were constructed based on 545 bp of CO1 gene from white, black, red, yellow, blue colors, and their combination. Several sequences were derived from GenBank for relevant species from different countries and the outgroup. The result showed that all samples are homolog, even to N. denticulata from China NC_023823.1.","PeriodicalId":8801,"journal":{"name":"Biodiversitas Journal of Biological Diversity","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139356237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity and cellulolytic activity of cellulase-producing bacteria isolated from the soils of two mangrove forests in Eastern Thailand","authors":"Tanapat Bamrungpanichtavorn, Sunisa Ungwiwatkul, P. Boontanom, Aiya Chantarasiri","doi":"10.13057/biodiv/d240728","DOIUrl":"https://doi.org/10.13057/biodiv/d240728","url":null,"abstract":"Abstract. Bamrungpanichtavorn T, Ungwiwatkul S, Boontanom P, Chantarasiri A. 2023. Diversity and cellulolytic activity of cellulase-producing bacteria isolated from the soils of two mangrove forests in Eastern Thailand. Biodiversitas 24: 3891-3902. The Southeast Asian countries hold the largest proportion of the world's mangrove area. Mangrove forests are a potential source for the isolation of economic microbial enzymes. Cellulases are a widely used microbial enzyme for cellulose degradation in various industries. Therefore, this study aimed to isolate, genetically identify, and enzymatically characterize cellulase-producing bacteria from the soils of two mangrove forests in Eastern Thailand. Twenty-six cellulase-producing bacteria were isolated and subsequently genotyped by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis of the 16S rRNA genes. Thirteen different RFLP patterns were obtained and genetically analyzed into six bacterial genera comprising Aeromonas, Bacillus, Chryseobacterium, Lysinibacillus, Pseudomonas, and Vibrio. The Bacillus species were the predominant cellulase-producing bacteria in the study sites. Moreover, the cellulase-producing Chryseobacterium and Lysinibacillus had hardly ever been reported. The Bacillus sp. strain RY08B was the most active cellulase-producing bacterium with 1.510±0.060 U/mL of CMCase activity. The optimum temperature and pH for the CMCase activity were determined to be 50°C at a pH of 7.0 with a thermal stability range of 25-50°C at a pH of 7.0. This bacterium could be applied in several environmentally friendly industries requiring mild conditions for their production processes.","PeriodicalId":8801,"journal":{"name":"Biodiversitas Journal of Biological Diversity","volume":"67 2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139356209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anatomical characteristics of the leaning stem in Pinus merkusii seedling","authors":"Nevlya Mia Safitri, Fanany Wuri Prastiwi, Syifa Salsabila, Yogi Feriawan, Alif Brilianto, W. D. Nugroho","doi":"10.13057/biodiv/d240717","DOIUrl":"https://doi.org/10.13057/biodiv/d240717","url":null,"abstract":"Abstract. Safitri NM, Prastiwi FW, Salsabila S, Feriawan Y, Brilianto A, Nugroho WD. 2023. Anatomical characteristics of the leaning stem in Pinus merkusii seedling. Biodiversitas 24: 3796-3802. Pinus merkusii Jungh. & de Vriese is one of the tropical conifer species. The species formed reaction wood called compression wood (CW) when the stem grows in an abnormal position. CW in stem wood is frequently found to cause several problems for utilization in the use of its products. However, there are few studies on the anatomical characteristics of reaction wood in tropical conifer. This study aimed to observe the differences in anatomical characteristics between compression (CW) and opposite (OW) woods in leaning the stem of P. merkusii seedlings and their anatomical differences compared to normal wood (NW). Approx. 1-year-old P. merkusii seedlings from a nursery in Trenggalek, East Java, Indonesia, that were naturally bent or tilted were used in the study. Anatomical characteristics of wood, namely the dimension of tracheids, rays, resin canals, and the proportion of cells were observed in the study. The results showed that CW of P. merkusii seedlings formed a rounded tracheid shape and intercellular space. The differences between CW and OW in P. merkusii seedlings were significant in terms of height of ray cells, tracheid proportion, and resin canal proportion. However, tracheid length, tracheid diameter, tracheid wall thickness, resin canal diameter and frequency, rays frequency, and ray proportion were not significantly different compared to OW and NW.","PeriodicalId":8801,"journal":{"name":"Biodiversitas Journal of Biological Diversity","volume":"93 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139356249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution and biodegradation analysis of polyvinyl chloride microplastic by indigenous bacteria isolated from Supit Urang Landfill, Malang, Indonesia","authors":"K. Rozana, S. Prabaningtyas, Dimas Ricko Widyatama","doi":"10.13057/biodiv/d240724","DOIUrl":"https://doi.org/10.13057/biodiv/d240724","url":null,"abstract":"Abstract. Rozana K, Prabaningtyas S, Widyatama DR. 2023. Distribution and biodegradation analysis of polyvinyl chloride microplastic by indigenous bacteria isolated from Supit Urang Landfill, Malang, Indonesia. Biodiversitas 24: 3853-3859. Microplastic waste is one of the most common forms of environmental pollution. Indonesia is the world's fourth largest contributor of plastic waste. One type of plastic that accumulates in the environment is Polyvinyl Chloride (PVC). Various attempts or methods have been developed to reduce PVC plastic waste, one of which is with the help of using indigenous bacteria. This research is essential in determining the best optimization method for PVC biodegradation agents through molecular and bioinformatic approaches. Indigenous bacteria were isolated from Supit Urang Landfill, Malang City, with three dilution levels (10-4, 10-5, and 10-6). The three highest isolates were taken for further biodegradation tests for 30 days and were identified based on the 16S rRNA gene. Then, the BLAST results were made into a phylogenetic tree to determine the kinship of each species. The isolation results obtained 17 bacterial isolates, selected by initial biodegradation screening for 10 days. Isolate K4 has a degradation percentage of 1.61±0.007379%. While isolates K4 and K15 have a biodegradation potential of 3.04±0.001861%. and 1.90±0.005576%. The BLAST results showed that K4 isolate had a 99% similarity to Staphylococcus capitis, K14 had a 99% similarity to Bacillus subtilis, and K15 had a 100% similarity to Acinetobacter pittii.","PeriodicalId":8801,"journal":{"name":"Biodiversitas Journal of Biological Diversity","volume":"72 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139356394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}