BiocelebesPub Date : 2023-06-27DOI: 10.22487/bioceb.v17i1.16440
Umrah Umrah, A. Hasanuddin, Eny Yuniati, Trismansyah Trismansyah
{"title":"PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus) PADA MEDIA DASAR LIMBAH KULIT SINGKONG","authors":"Umrah Umrah, A. Hasanuddin, Eny Yuniati, Trismansyah Trismansyah","doi":"10.22487/bioceb.v17i1.16440","DOIUrl":"https://doi.org/10.22487/bioceb.v17i1.16440","url":null,"abstract":"Cassava peel used as the basic medium for the growth of mycelium of white oyster mushroom (Pleurotus ostreatus). The purpose of this study : to determine the growth of white oyster mushroom mycelium (P. ostreatus) and to determine the ratio of basic media that can provide the best growth of white oyster mushroom mycelium. This study was designed in a completely randomized design (CRD) consisting of seven treatments and three replications, namely P1 (100% cassava peel base medium), P2 (MD 80% + SG 20%), P3 (MD 60% + SG 40% ), P4 (MD 40% + SG 60%), P5 (MD 80% + SG 10%), P6 (SG 100%), P7 (70% sawdust, 20% rice bran + 10% corn flour). The results of observation this research of the fastest growth at P4, P5 and the lowest at P6, were seen from the observation of mycelium growth. Colony stages on Potato Sucrose Agar (PSA) media, the results obtained were the highest number of colonies (CFU) in P1 treatment 1.83 x 1012 CFU/g and the lowest in P6 treatment 0.8 x 1012 CFU/g. The fastest storage time in treatment P4, P5 and the lowest in treatment P6.","PeriodicalId":8780,"journal":{"name":"Biocelebes","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87204366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiocelebesPub Date : 2023-06-27DOI: 10.22487/bioceb.v17i1.16441
Yulius Duma, A. Rosyid, Mobius Tanari, Mardiah Mangun, D. Damry
{"title":"SUBTITUSI PAKAN KONVENSIONAL DENGAN PAKAN NON-KONVENSIONAL PADA SATWA TARSIUS (Tarsius dianae) YANG DIPELIHARA DALAM KANDANG","authors":"Yulius Duma, A. Rosyid, Mobius Tanari, Mardiah Mangun, D. Damry","doi":"10.22487/bioceb.v17i1.16441","DOIUrl":"https://doi.org/10.22487/bioceb.v17i1.16441","url":null,"abstract":"This research was conducted in order to know the conventional feed of tarsier could be substituted by non-conventional feed such as fish and chicken. The research showed that the conventional feed could be susbstituted with fish and chicken without annoying the comfortable of tarsier’s life.","PeriodicalId":8780,"journal":{"name":"Biocelebes","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87145637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiocelebesPub Date : 2023-06-27DOI: 10.22487/bioceb.v17i1.16350
Hassanahtul Wulan Wulan
{"title":"PENGARUH PERBEDAAN KONSENTRASI GARAM TERHADAP JUMLAH BAKTERI ASAM LAKTAT (BAL) PADA SAUERKRAUT DARI FERMENTASI SAWI PUTIH (Brassica rapa L. ssp. pekinensis.)","authors":"Hassanahtul Wulan Wulan","doi":"10.22487/bioceb.v17i1.16350","DOIUrl":"https://doi.org/10.22487/bioceb.v17i1.16350","url":null,"abstract":"Fermentasi adalah bagian dari bioteknologi yang merupakan suatu proses pemanfaatan mikroorganisme untuk menghasilkan sebuah produk yang dapat digunakan oleh manusia. Mikroorganisme yang berkontribusi besar adalah bakteri probiotik. Probiotik didefinisikan sebagai mikroorganisme hidup dalam bahan pangan yang apabila dikonsumsi dalam jumlah yang cukup akan memberikan manfaat kesehatan saluran pencernaan. Salah satu bekteri tersebut adalah bakteri asam laktat. Dengan memperbanyak produksi BAL maka akan bermanfaat dalam pengelohan makanan yang bersifat probiotik. Metode yang dapat digunakan dalam perbanyakan bakteri tersebut salah satunya dengan cara mengisolasi bakteri. Dalam hal ini, bakteri asam laktat dapat di isolasi dari hasil fermentasi, salah satu olahan fermentasi yaitu sauerkreut. Penelitian ini bertujuan untuk mengetahui pengaruh perbedaan konsentrasi garam terhadap jumlah BAL pada hasil fermentasi sawi putih (Brassica rapa L. ssp. pekinensis.) yang diolah menjadi sauerkreut. Penelitian ini menggunakan metode isolasi untuk menghitung jumlah bakteri. Identifikasi jumlah BAL konsentrasi garam yang efektif dalam membantu pertumbuhan BAL adalah 2,5% (kontrol, urutan kedua yaitu pada konsentrasi 3% dan yang paling sedikit terdapat pada konsentrasi 2% .","PeriodicalId":8780,"journal":{"name":"Biocelebes","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90016416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiocelebesPub Date : 2023-06-27DOI: 10.22487/bioceb.v17i1.16190
Ayu Reski Wahyuni Ayu
{"title":"EKSPLORASI TUMBUHAN HERBA BERMANFAAT OBAT OLEH MASYARAKAT TOLAKI DI DESA WATUKILA KECAMATAN LASOLO KABUPATEN KONAWE UTARA","authors":"Ayu Reski Wahyuni Ayu","doi":"10.22487/bioceb.v17i1.16190","DOIUrl":"https://doi.org/10.22487/bioceb.v17i1.16190","url":null,"abstract":"The title of this research is “Exploration of Medicinal Herb Plants by the Tolaki Community in Watukila Village, Lasolo District, North Konawe Regency, Southeast Sulawesi. This research was conducted in September 2020 in Watukila Village, Lasolo District, North Konawe Regency, Southeast Sulawesi. The purpose of this study was to explore the types of herbaceous plants, the organs of herbaceous plants that are used as traditional medicine, and how to use and efficacy of herbaceous plants. The identification process was carried out at the Taxonomy Laboratory, Faculty of Biology Education, Halu Oleo University. The method used in this study is an exploratory and descriptive survey consisting of field surveys and interviews using a questionnaire. The results showed that there were 30 types of herbaceous plants from 20 families traditionally used as medicine. Organ leaves are most widely used as medicine with a percentage of 58%, the method used the most is boiled with a percentage of 53%.","PeriodicalId":8780,"journal":{"name":"Biocelebes","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84644931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiocelebesPub Date : 2023-06-27DOI: 10.22487/bioceb.v17i1.16439
Abi Gael Sidon, Wahyu Harso, Umrah Umrah, Orryani Lambui
{"title":"PERBANDINGAN JUMLAH BAKTERI TANAH PADA HUTAN BERDAUN LEBAR DAN HUTAN BERDAUN JARUM PADA KEDALAMAN TANAH YANG BERBEDA","authors":"Abi Gael Sidon, Wahyu Harso, Umrah Umrah, Orryani Lambui","doi":"10.22487/bioceb.v17i1.16439","DOIUrl":"https://doi.org/10.22487/bioceb.v17i1.16439","url":null,"abstract":"The number of soil microorganisms that play a role in the organic matter decomposition process can be affected by litter quantity and quality. Lignin content is higher in needleleaf than in broadleaf. The study aimed to determine the number of bacteria found in the broadleaf forest and needleleaf forest soil at different soil depths. The soil was collected at depths of 0-5 cm, 10-15 cm, and 20-25 cm at a distance of 1.5 m from the canopy of both broadleaf and coniferous forests where their locations are close. The results showed that the soil organic matter of broadleaf forests was higher than needleleaf forests, especially at depths of 0-5 cm and 10-15 cm. The number of bacteria on broadleaf forest soil is higher than on needleleaf forest soil, especially at 0-5 cm depth. The organic matter in both forest soils decreases with the soil depth. A positive correlation is obtained between the quantity of organic matter and the number of bacteria. The quantity and quality of organic matter in the soil will affect the number of bacteria.","PeriodicalId":8780,"journal":{"name":"Biocelebes","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78945439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiocelebesPub Date : 2023-06-27DOI: 10.22487/bioceb.v17i1.16269
Aan Febriawan
{"title":"STRUKTUR DAN KOMPOSISI VEGETASI MANGROVE DI KAWASAN PERKEBUNAN DESA PINOTU","authors":"Aan Febriawan","doi":"10.22487/bioceb.v17i1.16269","DOIUrl":"https://doi.org/10.22487/bioceb.v17i1.16269","url":null,"abstract":"Penelitian ini bertujuan mendeskripsikan struktur dan komposisi vegetasi mangrove tumbuh secara alami di kawasan perkebunan di Desa Pinotu. Metode yang digunakan adalah metode transek, dengan peletakan nested plot secara purposive berdasarkan pertimbangan tingkat pertumbuhan, yakni plot 1 x 1 m untuk anakan, 5 x 5 m untuk pancang dan 10 x 10 m untuk pohon. Selanjutnya dilakukan perhitungan nilai penting, sehingga diketahui jenis yang bernilai penting rendah yaitu Aegiceras floridum R. & S. 11,7 % pada tingkat anakan, Sonneratia alba J.E. Smith 10,5 % pada tingkat pancang, dan Pongamia pinnata (L.) Pierre 2,8 % pada tingkat pohon. Sementara jenis yang bernilai penting tinggi adalah Avicennia marina (Forsk.) Vierh. 114,8 % pada tingkat anakan, Rhizophora apiculata Bl. 168,4 % pada tingkat pancang dan Avicennia marina (Forsk.) Vierh. 153,9 % pada tingkat pohon. Berdasarkan hasil yang diperoleh, diketahui komposisi penyusun vegetasi hutan mangrove terdiri atas 9 jenis yang tergolong dalam 6 suku, yaitu Aegiceras corniculatum (L.) Blanco, Aegiceras floridum R. & S., Pongamia pinnata (L.) Pierre, Sonneratia alba J.E. Smith, Bruguiera gymnorrizha (L.) Lamk., Rhizophora apiculata Bl., Rhizophora mucronata Lmk., Xylocarpus moluccensis (Lamk) Roem. dan Avicennia marina (Forsk.) Vierh.","PeriodicalId":8780,"journal":{"name":"Biocelebes","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90456892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiocelebesPub Date : 2023-06-27DOI: 10.22487/bioceb.v17i1.16328
S. Siska
{"title":"JUMLAH BAKTERI ASAM LAKTAT (BAL) PADA SAUERKRAUT DARI KUBIS UNGU (Brasica oleracea var. capitata L.f. rubra) DENGAN KONSENTRASI GARAM YANG BERBEDA","authors":"S. Siska","doi":"10.22487/bioceb.v17i1.16328","DOIUrl":"https://doi.org/10.22487/bioceb.v17i1.16328","url":null,"abstract":"Bakteri asam laktat merupakan kelompok bakteri yang menghasilkan asam laktat sebagai produk utama dalam fermentasi. BAL tergolong dalam bakteri gram positif, tidak membentuk spora, berbentuk coccus atau basil dan pada umumnya bersifat katalase negatif, dan membutuhkan suhu mesofilik. BAL dapat diisolasi dari berbagai olahan pangan fermentasi salah satunya adalah sauerkraut. Sauerkraut (kubis asam) adalah makanan khas Jerman yang terbuat dari kubis yang diiris halus dan difermentasikan. Proses fermentasi sauerkraut dilakukan dengan penambahan garam. Konsentrasi garam yang optimal pada fermentasi sayuran dan buah yaitu antara 2–3%. Sauerkraut dapat dibuat dengan menggunakan berbagai jenis sayuran seperti kubis, rebung, kangkung, genjer, dan sawi. Kubis atau kol memiliki beberapa jenis salah satunya yaitu kubis ungu. Kubis ungu (Brassica oleracea var. capitata L.f. rubra) merupakan salah satu jenis kubis yang memiliki warna ungu dan tinggi akan antioksidan. Penelitian ini bertujuan untuk mengetahui jumlah bakteri asam laktat pada sauerkraut dari kubis ungu dengan konsentrasi garam yang berbeda. Konsentrasi garam yang dipakai adalah 2%, 2,5%, dan 3%. Penelitian ini merupakan penelitian deskriptif, yang mengisolasi BAL yang terdapat pada sauerkraut. Hasil penelitian ini diperoleh total BAL yang didapatkan pada setiap konsentrasi garam yaitu 2%, 2,5%, dan 3% cenderung menurun yaitu 27 x 107 cfu/μl, 14,5 x 107 cfu/μl, 9,7 x 107 cfu/μl. Total BAL tertinggi yaitu 27 x 107 cfu/μl pada konsentrasi garam 2% dan total BAL terendah yaitu 9,7 x 107 cfu/μl pada konsentrasi garam 3%.","PeriodicalId":8780,"journal":{"name":"Biocelebes","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82134336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiocelebesPub Date : 2023-06-27DOI: 10.22487/bioceb.v17i1.16405
Esna Dilli Novianto, Nurul Anindyawati, Nindya Nur Aina Kasih
{"title":"PENGARUH PEMBERIAN LIMBAH BAGLOG JAMUR TIRAM PADA PERTUMBUHAN DAN HASIL TANAMAN CAISIM (Brassica juncea L.)","authors":"Esna Dilli Novianto, Nurul Anindyawati, Nindya Nur Aina Kasih","doi":"10.22487/bioceb.v17i1.16405","DOIUrl":"https://doi.org/10.22487/bioceb.v17i1.16405","url":null,"abstract":"Caisim (Brassica juncea L.) is one of the most popular vegetable crops in the community. However, the production of caisim is not commensurate with the amount of consumer demand. Currently, one of the efforts made by farmers is to increase caisim production by adding inorganic fertilizers. This method can leave residues so that other alternatives are needed, such as the use of organic fertilizers that are more environmentally friendly. One of the potential sources of organic matter is oyster mushroom baglog waste because it contains a number of nutrients that can act as soil improvement materials. This study aims to study the effect of adding oyster mushroom baglog waste to the growth and yield of caisim (Brassica juncea L.) plants. This study used a factorial Completely Randomized Block Design with 4 treatments and 5 blocks. The treatments consisted of 0 g, 300 g, 600 g and 900 g of baglog oyster mushroom waste. The data obtained were analyzed for variance and continued with the orthogonal polynomial test. The results showed that the control treatment was better than the other treatments. It is suspected that the oyster mushroom baglog waste did not undergo a good fermentation process so that the content of C-organic, N, P and K was low which resulted in fewer nutrients being available for plants. Oyster mushroom baglog waste is not recommended for caisim cultivation practices.","PeriodicalId":8780,"journal":{"name":"Biocelebes","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78678307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BiocelebesPub Date : 2022-12-31DOI: 10.22487/bioceb.v16i2.16112
Maria Wigati, N. Amini, Esna Dilli Novianto
{"title":"INOVASI UBI KAYU TRANSGENIK MENGGUNAKAN TEKNOLOGI CRISPR-CAS9 SEBAGAI UPAYA PEMENUHAN KEBUTUHAN PANGAN MASA DEPAN","authors":"Maria Wigati, N. Amini, Esna Dilli Novianto","doi":"10.22487/bioceb.v16i2.16112","DOIUrl":"https://doi.org/10.22487/bioceb.v16i2.16112","url":null,"abstract":"Jumlah penduduk yang kian meningkat menyebabkan kebutuhan pangan terus meningkat. Hal ini menyebabkan angka ketergantungan akan beras juga meningkat. Padahal hingga saat ini, untuk memenuhi kebetuhan bahan pangan beras, pemerintah masih harus melakukan impor. Untuk itu sebagai upaya menurunkan ketergantungan konsumsi beras masyarakat Indonesia, pemerintah menggalakkan program diverfisikasi pangan. Salah-satu komoditas yang difokuskan dalam program ini yaitu ketela pohon atau ubi kayu. Namun, dalam pemenuhan kebutuhan gizi, ketela pohon masih memiliki kandungan protein yang rendah. Untuk itu dilakukan genome editing dengan teknik CRISPR-Cas9. CRISPR-Cas9 ini adalah teknologi yang bekerja untuk pengeditan genom dengan kemampuan menemukan tempat tertentu di DNA, untuk memotong sebagian kecil DNA di tempat itu, dan mengedit DNA tersebut. Penerapan teknik CRISPR-Cas9 terhadap ketela pohon menghasilkan tanaman yang mengandung protein yang lebih tinggi.","PeriodicalId":8780,"journal":{"name":"Biocelebes","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90654050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"STUDI TUMBUHAN BERKHASIAT OBAT PADA SUKU DONDO DI KECAMATAN OGODEIDE, KABUPATEN TOLITOLI, PROVINSI SULAWESI","authors":"Syariful Anam, Agustinus Widodo, Muhamad Rinaldhi Tandah","doi":"10.22487/bioceb.v16i2.16063","DOIUrl":"https://doi.org/10.22487/bioceb.v16i2.16063","url":null,"abstract":"The Dondo Tribe is an indigenous tribe in Ogodeide District. The Dondo Tribe still uses traditional medicine to treat various diseases. The high trust of the Dondo people towards the use of traditional medicine makes people prefer to use traditional medicine. This study aimed to determine the types, parts, and methods of use of plants that have been used as traditional medicine by the bolrians of the Dondo tribe, Ogodeide District, Tolitoli Regency, Central Sulawesi Province. This type of research was descriptive qualitative and the sampling technique was purposive sampling, with structured interview techniques on 11 informants by filling out questionnaires with supporting documentation. The results of the study found that as many as 40 species of plants used as traditional medicine by the bolrians of the Dondo tribe in Ogodeide District which are still preserved today. The percentages of the most widely used plant parts were leaves 55%, stems 11%, rhizomes 11%, roots 5%, fruit 5%, herbs 5%, bark 2%, twigs 2%, tubers 2% and flowers 2%. The percentage of how to use plants as traditional medicine is 65% taken, 26% affixed, 3% seen, 3% washing and 3% smeared.","PeriodicalId":8780,"journal":{"name":"Biocelebes","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88278049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}