Biochemical and biophysical research communications最新文献_第5页

Graphene oxide in low concentrations can change mitochondrial potential, autophagy, and apoptosis paths in two strains of invertebrates with different life strategies 低浓度氧化石墨烯可以改变两种不同生活策略的无脊椎动物的线粒体电位、自噬和凋亡路径。

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2024-10-24 DOI: 10.1016/j.bbrc.2024.150898

Barbara Flasz , Agnieszka Babczyńska , Monika Tarnawska , Amrendra K. Ajay , Andrzej Kędziorski , Łukasz Napora-Rutkowski , Maria Augustyniak

{"title":"Graphene oxide in low concentrations can change mitochondrial potential, autophagy, and apoptosis paths in two strains of invertebrates with different life strategies","authors":"Barbara Flasz , Agnieszka Babczyńska , Monika Tarnawska , Amrendra K. Ajay , Andrzej Kędziorski , Łukasz Napora-Rutkowski , Maria Augustyniak","doi":"10.1016/j.bbrc.2024.150898","DOIUrl":"10.1016/j.bbrc.2024.150898","url":null,"abstract":"<div><div>Nanoparticles, like graphene oxide (GO), are particles with unique physiochemical properties that enable their wide application in various areas of life. The effects of GO on individual cell organelles like mitochondria and the effects of interactions are worth investigating, as they can activate multiple cellular processes, such as autophagy or apoptosis. Mitochondrial injury plays an essential role in the majority of cell death routines. In the project, we investigated cell health status measured as mitochondrial inner membrane depolarization, autophagy, and apoptosis induction during long-term GO administration in food (0.02 μg g<sup>−1</sup> and 0.2 μg g<sup>−1</sup> of food). Two unique <em>Acheta domesticus</em> strains that differ in life strategy were used: wild-type and long-lived at three different life stages (larva, young adult, mature adult). The changes in mitochondrial <em>trans</em>-membrane potential were marked in the wild-type strain. The autophagy was lower in all GO-treated groups in both strains, and the apoptosis was lower in both strains in the mature adult crickets. Low GO concentrations treatment for the whole life, despite mitochondrial dysfunction, may lead to inhibition of autophagy and apoptosis by arresting the cell cycle for the duration of repair, and other repair tools are involved in the process of restoring homeostasis.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142520893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tissue factor pathway inhibitor-2 inhibits integrin β1 activation and focal adhesion formation and suppresses peritoneal ovarian cancer dissemination in mice 组织因子通路抑制剂-2能抑制整合素β1的激活和病灶粘附的形成，并抑制小鼠腹膜卵巢癌的扩散。

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2024-10-24 DOI: 10.1016/j.bbrc.2024.150890

Yukihide Ota , Mari Uomoto , Shiro Koizume , Shinya Sato , Daisuke Hoshino , Mitsuyo Yoshihara , Yoshiyasu Nakamura , Hiroko Tadokoro , Shohei Myoba , Norihisa Ohtake , Etsuko Miyagi , Yohei Miyagi

{"title":"Tissue factor pathway inhibitor-2 inhibits integrin β1 activation and focal adhesion formation and suppresses peritoneal ovarian cancer dissemination in mice","authors":"Yukihide Ota , Mari Uomoto , Shiro Koizume , Shinya Sato , Daisuke Hoshino , Mitsuyo Yoshihara , Yoshiyasu Nakamura , Hiroko Tadokoro , Shohei Myoba , Norihisa Ohtake , Etsuko Miyagi , Yohei Miyagi","doi":"10.1016/j.bbrc.2024.150890","DOIUrl":"10.1016/j.bbrc.2024.150890","url":null,"abstract":"<div><div>Tissue factor pathway inhibitor-2 (TFPI2) is a Kunitz-type serine protease inhibitor and an ovarian clear cell carcinoma (CCC) biomarker. <em>TFPI2</em> is expressed in several cancers and exerts tumor-suppressive effects; however, the role of TFPI2 in the CCC cell phenotype remains unclear. Therefore, in this study, we investigated the function of <em>TFPI2</em> by establishing a gene knockout (KO) in ES-2 CCC cells and observed the change in phenotypes <em>in vitro</em> and <em>in vivo</em>. <em>TFPI2</em> KO inhibited ES-2 cell proliferation, increased extracellular matrix protein adhesion, enhanced focal adhesion formation and activated integrin β1 cell surface clustering <em>in vitro</em>, and markedly increased ES-2 tumor growth and dissemination in the peritoneal cavity of a mouse xenograft model. These findings suggest a novel function of <em>TFPI2</em> expression in suppressing the formation of focal adhesions in CCC cells, potentially by activating integrin β1. This function plays a role in the peritoneal growth characteristics of CCC cells.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anxiolytic- and antidepressive-like effects of harmaline in mice are mediated via histamine H3 receptor blockade 哈马灵对小鼠的抗焦虑和抗抑郁类似效应是通过组胺 H3 受体阻断介导的。

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2024-10-23 DOI: 10.1016/j.bbrc.2024.150879

Fatemeh Khakpai , Seyed Parsa Golshani , Sakineh Alijanpour , Mohaddeseh Ebrahimi-Ghiri , Mohammad-Reza Zarrindast

{"title":"Anxiolytic- and antidepressive-like effects of harmaline in mice are mediated via histamine H3 receptor blockade","authors":"Fatemeh Khakpai , Seyed Parsa Golshani , Sakineh Alijanpour , Mohaddeseh Ebrahimi-Ghiri , Mohammad-Reza Zarrindast","doi":"10.1016/j.bbrc.2024.150879","DOIUrl":"10.1016/j.bbrc.2024.150879","url":null,"abstract":"<div><div>Many neuropsychiatric disorders can be caused by neurotransmitter dysfunction. Experimental studies have demonstrated that histamine and the harmaline affect physiological processes through interaction with other neurotransmitter systems. The objective of these experiments was to investigate the involvement of the histaminergic system in the effects of harmaline on anxiety- and depressive-related effects in male NMRI mice. Behavioral tests were employed to evaluate anxiety-related symptoms (elevated plus maze; EPM), depressive-like symptoms (forced swim test; FST), and cognitive decline (step-down test). The histamine H3 receptor (H3R) agonist α-methylhistamine dihydrobromide (α-MH; 5 mg/kg, i.p.) had anxiolytic- and depressive-like effects, while the H3R antagonist thioperamide (10 mg/kg, i.p.) showed an antidepressive-like property. The subthreshold dose of α-MH resulted in an increase in the tendency of mice treated with the harmaline (2.5 mg/kg) to remain in the EPM open-arms. A subthreshold dose of thioperamide (5 mg/kg) increased the time spent in the open-arms in mice treated with harmaline (2.5 and 5 mg/kg) while a high dose of harmaline decreased the immobility time. Furthermore, two higher doses of harmaline resulted in a reduction in the number of open-arm entries. Similarly, mice administered with thioperamide and <em>a</em> low dose of harmaline decreased locomotor activity in the EPM. Ultimately, the combined thioperamide and harmaline did not impair memory retrieval of mice. These experiments demonstrate that the histaminergic system is implicated in the anxiety- and depressive-related effects of harmaline. The combination of thioperamide and harmaline is effective in treating anxiety and depression without having an adverse effect on memory formation.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142520892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rho-associated, coiled-coil-containing protein kinase 2 regulates expression of mineralocorticoid receptor to mediate sodium reabsorption in mice Rho相关含线圈蛋白激酶2调节矿质皮质激素受体的表达，从而介导小鼠的钠重吸收。

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2024-10-23 DOI: 10.1016/j.bbrc.2024.150874

Kensuke Sekiguchi , Keiichiro Matoba , Yosuke Nagai , Satoru Nagao , Shinji Ohashi , Etsuko Mitsuyoshi , Takeshi Hayashi , Daiji Kawanami , Tamotsu Yokota , Hirotaka Shibata , Kazunori Utsunomiya , Rimei Nishimura

引用次数: 0

Cyanide mediated conformational changes resulted in the displacement of sulfate ion from the active site of bovine pancreatic ribonuclease A 氰化物介导的构象变化导致硫酸根离子从牛胰腺核糖核酸酶 A 的活性位点移位

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2024-10-23 DOI: 10.1016/j.bbrc.2024.150868

Nayab Shah , Zeeshan Akbar , Malik Shoaib Ahmad

引用次数: 0

Decreased mitochondrial translation confers 3,3′-Diindolylmethane resistance to Schizosaccharomyces pombe 线粒体翻译减少使小鼠具有 3,3'-Diindolylmethane 抗性

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2024-10-23 DOI: 10.1016/j.bbrc.2024.150864

Kaiyu Wang , Hideto Nagai , Samiul Alam Rajib , Yorifumi Satou , Masaru Ueno

{"title":"Decreased mitochondrial translation confers 3,3′-Diindolylmethane resistance to Schizosaccharomyces pombe","authors":"Kaiyu Wang , Hideto Nagai , Samiul Alam Rajib , Yorifumi Satou , Masaru Ueno","doi":"10.1016/j.bbrc.2024.150864","DOIUrl":"10.1016/j.bbrc.2024.150864","url":null,"abstract":"<div><div>3,3′-Diindolylmethane (DIM), a compound derived from natural fruits and vegetables, is widely recognized for its anti-cancer activity. However, its action mechanisms remain ambiguous. In this study, to study the molecular mechanism of 3,3′-Diindolylmethane, we identified a novel mutation in the gene of mitochondrial translation elongation factor EF-Ts (<em>tsf1</em><sup><em>+</em></sup><em>)</em>, a key factor in mitochondrial protein translation, that conferred DIM resistance to <em>Schizosaccharomyces pombe</em>. The <em>tsf1Δ</em> also conferred DIM resistance. Decreased mitochondrial translation was found to be responsible for conferring DIM resistance to <em>Schizosaccharomyces pombe</em>, as the cells gained DIM resistance after treatment with chloramphenicol, a specific mitochondrial translation inhibitor. Notably, <em>tsf1</em>Δ conferred DIM resistance in the absence of either autophagy-related protein, Atg7, or nuclear envelope protein, Lem2, two proteins that have been reported to be required for cell survival in the presence of DIM. Overall, this study revealed novel biological functions of DIM and highlighted its potential as an anti-cancer agent.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TLR2–EGR1 signaling axis modulates TGFβ1-induced differentiation of fibroblasts into myofibroblasts in pulmonary fibrosis TLR2-EGR1信号轴调节TGFβ1诱导的肺纤维化中成纤维细胞向肌成纤维细胞的分化。

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2024-10-23 DOI: 10.1016/j.bbrc.2024.150836

Euitaek Jung , Tae Yoon Kim , Junekyu Han , Kye Young Lee , Soon Young Shin

{"title":"TLR2–EGR1 signaling axis modulates TGFβ1-induced differentiation of fibroblasts into myofibroblasts in pulmonary fibrosis","authors":"Euitaek Jung , Tae Yoon Kim , Junekyu Han , Kye Young Lee , Soon Young Shin","doi":"10.1016/j.bbrc.2024.150836","DOIUrl":"10.1016/j.bbrc.2024.150836","url":null,"abstract":"<div><div>Pulmonary fibrosis is a progressive lung condition characterized by the excessive activation of myofibroblasts. Transforming growth factor beta 1 (TGFβ1) plays a crucial role in the differentiation of fibroblasts into myofibroblasts. In addition, toll-like receptor 2 (TLR2), known for its role in immune responses, contributes to pulmonary fibrosis by promoting myofibroblast differentiation. However, the interplay between TGFβ1 and TLR2 signaling pathways in myofibroblast differentiation has remained elusive. In the present study, we investigated the involvement of TLR2 in TGFβ1-induced fibroblast differentiation into myofibroblasts using IMR-90 human pulmonary fibroblasts as a model cell line. We found that TLR2 activation induced myofibroblast differentiation by enhancing the expression of early growth response 1 (EGR1) via the mitogen-activated protein kinase (MAPK) signaling pathway. Elevated EGR1 levels were detected in the lung tissues of a bleomycin (BLM)-induced mouse model of pulmonary fibrosis. Moreover, the administration of tomaralimab, an antagonistic anti-TLR2 antibody, reduced the EGR1 expression and collagen deposition. Altogether, targeting the TLR2–EGR1 pathway could be a promising therapeutic approach for pulmonary fibrosis by blocking TGFβ1-induced myofibroblast differentiation.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142520895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elevated circulating HHIP levels in patients with metabolic syndrome 代谢综合征患者体内循环 HHIP 水平升高

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2024-10-23 DOI: 10.1016/j.bbrc.2024.150877

Jingwei Lei , Yu Yang , Yerui Lai , Dongfang Liu , Cong Wang , Weiwei Xu , Ke Li , Shengbing Li , Mengliu Yang , Ling Li

{"title":"Elevated circulating HHIP levels in patients with metabolic syndrome","authors":"Jingwei Lei , Yu Yang , Yerui Lai , Dongfang Liu , Cong Wang , Weiwei Xu , Ke Li , Shengbing Li , Mengliu Yang , Ling Li","doi":"10.1016/j.bbrc.2024.150877","DOIUrl":"10.1016/j.bbrc.2024.150877","url":null,"abstract":"<div><div>Abnormal fat accumulation can lead to metabolic syndrome (MetS), increasing the risk of diabetes and cardiovascular disease in MetS patients. Early identification of MetS risk is essential for effective disease prevention. Using bioinformatics methods, we sought biomarkers for MetS. After analyzing the GSE9624 and GSE15524 datasets, we identified three commonly differentially expressed genes: COX7A1, PRR12, and HHIP. Subsequently, we validated the expression of these DEGs using the GSE65540 dataset. Quantitative PCR and immunoblotting confirmed significantly elevated HHIP expression in the adipose tissue of HFD-fed and ob/ob mice. Furthermore, a population-based cohort study demonstrated that serum HHIP levels were significantly greater in MetS patients than in healthy controls and were correlated with all MetS components. Receiver operating characteristic (ROC) curve analysis confirmed the robust predictive capacity of HHIP levels for metabolic syndrome, with an area under the curve (AUC) of 0.72 (95 % confidence interval: 0.68–0.78, P < 0.001). Binary logistic regression showed that the serum HHIP concentration was significantly associated with MetS even after adjusting for anthropometric and lipid profile variables. In conclusion, our findings demonstrate that changes in HHIP expression are significantly associated with adverse MetS indicators, indicating that HHIP can serve as a new biomarker for the diagnosis of MetS.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142534316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of infection by Mycoplasma arginini and Mycoplasma salivarium on the oncogenic properties of lung cancer cell line A549 精支原体和唾液支原体感染对肺癌细胞株 A549 致癌特性的影响

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2024-10-23 DOI: 10.1016/j.bbrc.2024.150878

S.E. Parfenyev , I.E. Vishnyakov , T.N. Efimova , A.A. Daks , O.Y. Shuvalov , O.A. Fedorova , E.V. Lomert , D.G. Tentler , S.N. Borchsenius , N.A. Barlev

引用次数: 0

Chromatin condensed domains revealed by AFM, and their transformation in mechanically deformed normal and malignant cell nuclei 原子力显微镜揭示的染色质凝聚结构域及其在机械变形的正常细胞核和恶性细胞核中的转变。

IF 2.5 3区生物学

Biochemical and biophysical research communications Pub Date : 2024-10-23 DOI: 10.1016/j.bbrc.2024.150861

V. Yu Bairamukov , A.V. Ankudinov , R.A. Kovalev , R.A. Pantina , S.V. Grigoriev , E. Yu Varfolomeeva

{"title":"Chromatin condensed domains revealed by AFM, and their transformation in mechanically deformed normal and malignant cell nuclei","authors":"V. Yu Bairamukov , A.V. Ankudinov , R.A. Kovalev , R.A. Pantina , S.V. Grigoriev , E. Yu Varfolomeeva","doi":"10.1016/j.bbrc.2024.150861","DOIUrl":"10.1016/j.bbrc.2024.150861","url":null,"abstract":"<div><div>It has been generally accepted that heterochromatin is represented by a regular, dense and closed structure, while euchromatin is open and sparse. Recent evidence indicates that chromatin is comprised of irregular nucleosome clutches compacted within the nucleus. Transcriptional events transform the chromatin architecture, resulting in appearance of 100–300 nm nucleosomal aggregates. Meanwhile, the current paradigm of chromatin architecture is largely fragmented. In this communication, we unraveled chromatin ultrastructure of normal and malignant cell nuclei through mechanical deformation of the nuclei and Atomic Force Microscopy (AFM) analysis of the resulting landscape. In human skin fibroblasts cell nuclei, nanodomains of about 16.5–33.5 nm were revealed. Hierarchical folding of the chromatin of normal nuclei was observed: the nanodomains formed irregular fiber-like structures that coalesced into the macroscale chromatin compartments. In fibrosarcoma cell nuclei DNA supercoiling domains (SDs) of about 66.3–113.0 nm, uniformly distributed within the nuclei, were revealed. Transformation of the morphology of the condensed chromatin domains through up- and downregulation of supercoiling was demonstrated.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142493897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0