Bing du xue bao = Chinese journal of virology最新文献_第6页

[The Complete Sequence Analysis of 18 Strains of Coxsackievirus A6 in Guangdong Province of China]. 广东省18株柯萨奇病毒A6全序列分析

Bing du xue bao = Chinese journal of virology Pub Date : 2016-09-01

Hanri Zeng, Jing Lu, Huanying Zheng, Xiaoli Chen, Leng Liu, Xue Guo, Changwen Ke, Hui Li

{"title":"[The Complete Sequence Analysis of 18 Strains of Coxsackievirus A6 in Guangdong Province of China].","authors":"Hanri Zeng, Jing Lu, Huanying Zheng, Xiaoli Chen, Leng Liu, Xue Guo, Changwen Ke, Hui Li","doi":"","DOIUrl":"","url":null,"abstract":"In this study, we examined the complete genome of coxsackievirus A6 (CVA6) from hand, foot, and mouth disease in Guangdong Province from 2013,and explored the genetic similarities and differences in epidemic and non-epidemic stains of CVA6.Eighteen strains of CVA6 were included in complete genome sequencing, and the sequences were subject to phylogenetic analysis,sequence alignment analysis and genetic recombination analysis using the software DNASTAR6.0,MEGA5.2and SimPlot3.5.1.The results showed that the complete genome of 18 Guangdong CVA6strains ranged from 7390bp to 7392bp.No insertions or deletions were detected in the coding region. There were several insertions and deletions in 5′UTR and 3′UTR.Phylogenetic analysis indicated that the nucleotide and amino acid sequence identity between the 18 complete genomes were 90.5%-99.6% and 97.5%-99.9%,respectively.The strains isolated in2013 could be further divided into two clusters, III and IV, while the strains isolated in 2011 were only present in the IV cluster. Genetic recombination analysis revealed that the Guangdong representative strain of CVA6,GD870/2013,had gene recombination in the P2 and P3regions,while the GD839/2013 strain did not show obvious genetic recombination. Genome-wide analysis of CVA6 revealed that there are two possible transmitted chains, III and IV, in epidemic strains from Guangdong Province in 2013.The transmitted chain Ⅲ originated from the strain with genetic recombination in the P2 and P3regions,whichwas completely different from the chain IV. Transmission of chain IV of CVA6 was only observed in the nonepidemic 2011 strain.","PeriodicalId":8776,"journal":{"name":"Bing du xue bao = Chinese journal of virology","volume":" ","pages":"566-73"},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40441995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Isolation and Identification of a Novel Cypovirus from Daphnis nerii]. 一种新型蛇形病毒的分离鉴定。

Bing du xue bao = Chinese journal of virology Pub Date : 2016-09-01

Zhigao Zhan, Yuzhou Xiao, Zhuorong Liu, Wenchao Zhang, Jinchang Wang, Hongxiu Wang, Limei Guan, Gang Yang, Zhao Huang, Liang Jin

{"title":"[Isolation and Identification of a Novel Cypovirus from Daphnis nerii].","authors":"Zhigao Zhan, Yuzhou Xiao, Zhuorong Liu, Wenchao Zhang, Jinchang Wang, Hongxiu Wang, Limei Guan, Gang Yang, Zhao Huang, Liang Jin","doi":"","DOIUrl":"","url":null,"abstract":"In order to develop a novel effective biological insecticide for controlling oleander hawk moth, a new pathogen was isolated from naturally diseased Daphnis nerii. Based on scanning electron microscopy, full-length amplification of cDNAs (FLAC), and phylogenetic analysis of genome segments 2and 10,the virus was identified as a new type of cypovirus (Da phnis nerii cypovirus [DnCPV]). Electrophoresis analysis showed that DnCPV had a genome comprising 10double-stranded RNA (dsRNA) segments, ranging from 892 to 4160bp.Using FLAC, the cDNAs from the 10 dsRNA segments of the new CPV were cloned and genome segments 2and 10 were sequenced. Sequencing results showed that segment 2 encoded RNA-dependent-RNA-polymerases (RdRps) and segment 10 encoded polyhedrin. These two segments shared conserved terminal sequences of AGUCAAA and AGC at the 5’and 3’ends,respectively.These conserved terminal sequences were not consistent with any of the known CPV types.Phylogenetic analysis of the RdRp and polyhedrin indicated that this CPV was more closely related to CPV type 19 and type 5than other CPV types. Based on the unique conserved terminal sequences and the electrophoresis pattern of the new virus, we tentatively named it DnCPV Nanchang isolate: DnCPV-NC.","PeriodicalId":8776,"journal":{"name":"Bing du xue bao = Chinese journal of virology","volume":"32 5","pages":"619-26"},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36308682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Advances in Avian Antiviral Innate Immune Effectors]. 禽抗病毒先天免疫效应物研究进展

Bing du xue bao = Chinese journal of virology Pub Date : 2016-09-01

Miao Zeng, Shun Chen, Mingshu Wang, Anchun Chen

引用次数: 0

[Analysis of Polymorphisms in the HCMV UL144 Gene in Clinical Isolates from Children with Asymptomatic Infection]. [无症状感染儿童临床分离HCMV UL144基因多态性分析]。

Bing du xue bao = Chinese journal of virology Pub Date : 2016-09-01

Gangqiang Guo, Shangdan Xie, Sisi Ye, Liang Zhang, Xiangwei Sun, Baoqing Li, Lifang Zhang, Xiangyang Xue

{"title":"[Analysis of Polymorphisms in the HCMV UL144 Gene in Clinical Isolates from Children with Asymptomatic Infection].","authors":"Gangqiang Guo, Shangdan Xie, Sisi Ye, Liang Zhang, Xiangwei Sun, Baoqing Li, Lifang Zhang, Xiangyang Xue","doi":"","DOIUrl":"","url":null,"abstract":"The purpose of this study was to examine polymorphisms in the human cytomegalovirus(HCMV)UL144gene in children with asymptomatic HCMV infection. PCR was performed to amplify the UL144 open reading frame(ORF)from urine specimens of asymptomatic HCMV-DNA positive children and both strands of the amplicon were sequenced. Sequence analysis was performed with software including BioEdit,DNAstar,Mega5.0and GeneDoc. Twenty-one of 50 clinical strains were successfully amplified and sequenced, giving a positive rate of detection of 42%.Nucleotide sequence homology ranged from 80.2%to100% and amino acid sequence homology ranged from 77.8%to 100%.The UL144 sequences were distributed among two genotypes, type A(47.61%)and type B(52.38%).The Expasy database was used to analyze the important functional motifs of the UL144 protein. These results revealed that there was a high level of conservation of post-translational modification sites including ASN, PKC, TNFR, and NCD3 G.UL144type B added a PROKAR-LIPOPROTEIN site and ZF-CTCHY site between amino acid residues 1 and 16 and between amino acid residues 30 and 96,respectively,as compared with type A.Compared to the UL144 gene from the Toledo strain, there was a high level of conservation in the CRD1 and CRD2of UL144 type A, while significantly more variability was observed in CRD1 and CRD2of UL144 type B. The transmembrane and cytoplasmic domains were highly conserved in both UL144 type A and type B. Variation in nucleotide sequences of UL144 type A and type B did not cause major changes to the predicted isoelectric point or secondary structure of the UL144 protein. The UL144 genotype of children with asymptomatic HCMV infection was divided into type A and type B, which was different from children with symptomatic HCMV infection.","PeriodicalId":8776,"journal":{"name":"Bing du xue bao = Chinese journal of virology","volume":" ","pages":"610-8"},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40531249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Identification of the Transcriptional Activity Domain of EV71 3D(pol)]. EV71 3D(pol)转录活性域的鉴定

Bing du xue bao = Chinese journal of virology Pub Date : 2016-09-01

Xiao Xu, Yunfang Yao, Jing Li, Keli Chai, Wentao Qiao, Juan Tan

引用次数: 0

[Analysis of Common Issues and Research Progress in Loop Mediated Isothermal Amplification]. [环介导等温扩增常见问题分析及研究进展]。

Bing du xue bao = Chinese journal of virology Pub Date : 2016-09-01

Zongyue Hu, Zhouheng Xu, Yiyu Lu

引用次数: 0

[Varieties and Mechanisms of Synergistic Factors Enhancing Baculovirus Infectivity]. 增强杆状病毒感染性的协同因子的种类和机制。

Bing du xue bao = Chinese journal of virology Pub Date : 2016-09-01

Linna Zhang, Wenfeng Chen, Xinming Yin, Wenming Li, Xiangyang Liu, Zhongxin Zhang

引用次数: 0

[Analysis of Egg-adapted Mutations in Influenza A H1N1pdm09 Viruses in Hubei Province of China, 2013-2014]. [2013-2014年湖北省甲型流感H1N1pdm09病毒卵适应突变分析]。

Bing du xue bao = Chinese journal of virology Pub Date : 2016-09-01

Bin Fang, Linlin Liu, Guojun Ye, Xiang Li, Xiao Yu, Yongzhong Jiang

{"title":"[Analysis of Egg-adapted Mutations in Influenza A H1N1pdm09 Viruses in Hubei Province of China, 2013-2014].","authors":"Bin Fang, Linlin Liu, Guojun Ye, Xiang Li, Xiao Yu, Yongzhong Jiang","doi":"","DOIUrl":"","url":null,"abstract":"Influenza A H1N1pdm09 Virus; Chicken embryo; Phylogenetic tree; Egg-adaptation; Antigenic site; Drug-resistance site; \u0000Abstract: To investigate egg-adapted mutation in influenza A H1N1pdm09 viruses isolated from the Hubei influenza surveillance network, a comparative analysis was performed of three influenza A H1N1pdm09 viruses isolated in chicken embryo and the corresponding MDCK cell-derived viruses. Analyses included examination of the phylogenetic tree, evolutionary rates, amino acid substitutions, egg-adapted mutation and homology modeling. We found differences between the egg-adapted viruses and MDCK cell-derived viruses based on phylogenetic trees and evolutionary rates; the viruses showed a trend of \" NA>HA>MP\". Four amino acid substitutions(Q223R,V527 I,M19Iand H275Y)were found in three egg-adapted viruses.Q223 Rand V527Iwere present in the haemagglutinin protein, while M19I and H275Y were detected in neuraminidase.The Q223R mutation changed the structure of antigenic sites between Sb and Ca2. H275Y is a classic neuraminidase resistance mutation. The results suggest that the egg-adapted mutations were introduced when influenza viruses were isolated in chicken embryos from the Hubei influenza surveillance network. These mutations may affect the selection of vaccine candidates and vaccine efficacy; therefore, monitoring of egg-adapted mutations should be strengthened in the influenza surveillance network.","PeriodicalId":8776,"journal":{"name":"Bing du xue bao = Chinese journal of virology","volume":" ","pages":"582-9"},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40441564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Construction of Recombinant Full-length Hepatitis E Virus Fused with EGFP and Assessment of Infectivity]. [EGFP融合重组戊型肝炎全长病毒的构建及传染性评价]。

Bing du xue bao = Chinese journal of virology Pub Date : 2016-09-01

Yunlong Li, Feiyan Long, Chenchen Yang, Wenhai Yu, Yanhong Bi, Jue Wang, Diancai Jiang, Fuchun Peng, Shenrong Jing, Fen Huang

{"title":"[Construction of Recombinant Full-length Hepatitis E Virus Fused with EGFP and Assessment of Infectivity].","authors":"Yunlong Li, Feiyan Long, Chenchen Yang, Wenhai Yu, Yanhong Bi, Jue Wang, Diancai Jiang, Fuchun Peng, Shenrong Jing, Fen Huang","doi":"","DOIUrl":"","url":null,"abstract":"The purpose of this study was to construct recombinant full-length hepatitis E virus(HEV)fused with enhanced green fluorescent protein(EGFP),and assess its infectivity in A549 cells. Two fragments from the full-length HEV genome and the EGFP gene were amplified by PCR. The EGFP gene was inserted downstream of the HEV ORF2 and then cloned into the pGEM® -7Zf(+)vector containing the T7 and SP6RNA polymerase promoters, producing pGEM-HEV-EGFP. The construction of the pGEM-HEV-EGFP recombinant plasmid was confirmed by restriction enzyme digest and sequencing. The pGEM-HEV-EGFP recombinant plasmid was transfected into A549 cells to assess infectivity using Lipofectamine. EGFP expression was observed at 24hpost-transfection,and expression of the HEV ORF2 was detected by immunofluorescence, confirming the presence of the HEV ORF2 and EGFP fusion protein. Cytopathic effects were observed at day seven post-transfection. The infectivity of pGEM-HEV-EGFP was confirmed by the presence of fluorescence after three continuous passages. The recombinant pGEM-HEV-EGFP vector was successfully constructed and effectively infected A549 cells, which will facilitate future studies on the mechanisms of HEV infection and pathogenesis.","PeriodicalId":8776,"journal":{"name":"Bing du xue bao = Chinese journal of virology","volume":" ","pages":"529-37"},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40532759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Cloning, Sequence Analysis and Expression of Recombinant E2 Protein of GB Virus C Genotype 7]. GB病毒C基因7型重组E2蛋白的克隆、序列分析及表达

Bing du xue bao = Chinese journal of virology Pub Date : 2016-09-01

Xiaoyu Yang, Yue Zhao, Yue Feng, Xueshan Xia

引用次数: 0