Bing du xue bao = Chinese journal of virology最新文献_第4页

[Research Progress in Virus Infection Altering Cellular Glucose Metabolism]. 病毒感染改变细胞糖代谢的研究进展

Bing du xue bao = Chinese journal of virology Pub Date : 2016-11-01

Shiwei Wu, Xiaozhe Fu, Qiang Lin, Lihui Liu, Hongru Liang, Zhibin Huang, Ningqiu Li

引用次数: 0

[Expression of Type-Ι Interferon Production Pathway-Related Genes Induced by Infection Due to Enterovirus 71 or Coxsackievirus A16 in Normal Human Airway Epithelial Cells]. [肠道病毒71或柯萨奇病毒A16感染诱导-Ι型干扰素产生途径相关基因在正常人气道上皮细胞中的表达]。

Bing du xue bao = Chinese journal of virology Pub Date : 2016-11-01

Jie Song, Yajie Hu, Jiaqi Li, Jingjing Wang, Lei Guo, Huiwen Zheng, Ruotong Ning, Lichun Wang, Qihan Li, Longding Liu

{"title":"[Expression of Type-Ι Interferon Production Pathway-Related Genes Induced by Infection Due to Enterovirus 71 or Coxsackievirus A16 in Normal Human Airway Epithelial Cells].","authors":"Jie Song, Yajie Hu, Jiaqi Li, Jingjing Wang, Lei Guo, Huiwen Zheng, Ruotong Ning, Lichun Wang, Qihan Li, Longding Liu","doi":"","DOIUrl":"","url":null,"abstract":"Hand, foot, and mouth disease(HFMD)is caused by mainly enterovirus 71(EV-A71)and coxsackievirus A16(CV-A16),and is a serious healthcare problem worldwide.EV-A71 infection is thought to progress readily to serious complications whereas CV-A16 infection, in general, results in mild symptoms and presents repeatedly. However, the underlying mechanisms leading to these differences are not known. We compared changes in expression of type-I interferon(IFN-I)-related genes in normal human bronchial epithelial(16HBE) cells. Gene-expression levels of TLR3,MAVS,MDA5,MyD88,IRF7,IFNαand IFNβwere elevated significantly after EVA71 infection.MDA5expression was increased markedly, and that of TLR3 and IRF3was decreased obviously after CV-A16 infection, but that of MAVS,MyD88,IFNαand IFNβdid not show significant differences. Viral copy number and viral titers suggested that CV-A16 replicates more efficiently than EV-A71 in 16HBE.These results suggest that IFN-I production pathway-related genes in response to infection by EV-A71 and CV-A16 have notable discrepancies. Such information could shine a light on the different manifestations caused by EV-A71 and CV-A16,and the mechanism of repeat infection by CV-A16.","PeriodicalId":8776,"journal":{"name":"Bing du xue bao = Chinese journal of virology","volume":"32 6","pages":"694-701"},"PeriodicalIF":0.0,"publicationDate":"2016-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36309591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Pathogenic Analyses of an Outbreak of Viral Encephalitis Caused by ECHO30 in Guazhou of Gansu Province, China]. 甘肃省瓜州市一起由ECHO30引起的病毒性脑炎暴发的病原学分析

Bing du xue bao = Chinese journal of virology Pub Date : 2016-11-01

Jianhua Chen, Zhen Zhu, Yushan Li, Haizhuo Wu, Haixia Liu, Xuxia Wang, Deshan Yu

{"title":"[Pathogenic Analyses of an Outbreak of Viral Encephalitis Caused by ECHO30 in Guazhou of Gansu Province, China].","authors":"Jianhua Chen, Zhen Zhu, Yushan Li, Haizhuo Wu, Haixia Liu, Xuxia Wang, Deshan Yu","doi":"","DOIUrl":"","url":null,"abstract":"We used molecular-biology methods to identify the pathogens that caused an outbreak of viral encephalitis in Guazhou (Gansu province, China)during June-August 2015.We also undertook molecular characterizations of these pathogens. A total of 132samples(14cerebrospinal fluid(CSF)samples;25throat swabs;66serum samples;27fecal samples)were collected from 74 patients during the outbreak of viral encephalitis. For CSF and serum samples, enzyme-linked immunosorbent assay immunoglobulin-M tests were undertaken to detect Japanese encephalitis viruses, enteroviruses, herpes simplex viruses, mumps viruses, and adenoviruses. Real-time polymerase chain reaction was done to detect enteroviruses(including coxsackievirus A16 and enterovirus 71) and the RNA of human adenoviruses. Then, viral isolation was carried out using HEp-2 and RD cells, and the entire VP1 region of positive viral isolates was amplified and sequenced. Finally, molecular characterizations of these pathogens were completed. Seventy two samples were identified as enteroviruses from 132 samples. Among them,71 were identified as echovirus(ECHO)30using enterovirus molecular typing. Japanese encephalitis viruses,herpes simplex viruses, mumps viruses, and adenoviruses were not detected.ECHO30 was isolated from 46 samples out of 29 patients.Similarities in nucleic acids among these ECHO30 isolates were 99.2%-100.0%.ECHO30 from Gansu province and other ECHO30 strains isolated in China since 2011 belonged to a same evolutionary branch.ECHO30 was the pathogen that caused the outbreak of viral encephalitis in Guazhou in 2015.ECHO30 from and Gansu province and ECHO30 isolated in China since 2011 belonged to the same evolutionary branch.","PeriodicalId":8776,"journal":{"name":"Bing du xue bao = Chinese journal of virology","volume":"32 6","pages":"721-6"},"PeriodicalIF":0.0,"publicationDate":"2016-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36308994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Progress in the Development of Animal Models Supporting Human Adenovirus Infection]. [支持人类腺病毒感染的动物模型的发展进展]。

Bing du xue bao = Chinese journal of virology Pub Date : 2016-11-01

Xikui Sun, Xingong Liu, Liqiang Feng

引用次数: 0

[Epidemiology Characteristics and Pathogen Surveillance of Hand, Foot and Mouth Disease in Guangdong Province, China, 2008～2015]. 广东省2008~2015年手足口病流行病学特征及病原监测[j]。

Bing du xue bao = Chinese journal of virology Pub Date : 2016-11-01

Tianjiao Ji, Xiaohua Tan, Leng Liu, Xinrui Gu, Li Liu, Huanying Zheng, Hanri Zeng, Qian Yang, Hui Li, Wenbo Xu

{"title":"[Epidemiology Characteristics and Pathogen Surveillance of Hand, Foot and Mouth Disease in Guangdong Province, China, 2008～2015].","authors":"Tianjiao Ji, Xiaohua Tan, Leng Liu, Xinrui Gu, Li Liu, Huanying Zheng, Hanri Zeng, Qian Yang, Hui Li, Wenbo Xu","doi":"","DOIUrl":"","url":null,"abstract":"To understand the epidemiological etiology characteristics of hand, foot and mouth disease(HFMD)in Guangdong province, and to explore the risk change trend of the whole province. By using the descriptive epidemiological methods, the whole province’s incidence trend, population distribution and pathogenic form of HFMD were analyzed with the HFMD surveillance data,population data and geographic information of Guangdong province from 2008 to 2015.The analysis results show: A total of 2,133,722 cases of HFMD, including 5,066 severe cases and 259 death cases were reported in Guangdong province from 2008 to 2015.All the cities of Guangdong had HFMD cases, especially the Pearl River Delta Regions, which were on high-risk areas. There were two peaks every year, with the main peak of incidence occurred in spring and summer, and the sub peak occurred in autumn.Most cases were children aged<5years old, the proportion of this group in overall infections, the severe and death cases were 90.58%,95.93%and 97.30%,respectively,while the proportion for the children less than 3years old were 77.32% and 81.85%,respectively. The incidence of this disease among men was higher than that of women. Dynamic changes were presented between different years and seasons:CV-A16 was more popular in 2009,and enterovirus that none EV-A71 and none CV-A16 were predominant strains in 2013 and 2015.Especially in 2015,the proportion of other EV ranged as high as 71.97%.Besides,EV-A71 was the absolute predominance pathogen within death cases and was important pathogen in severe cases. This study suggests that HFMD epidemiology and laboratory monitoring in Guangdong Province should be strengthened, and provides scientific data support for further improvement of HFMD prevention and control strategies in Guangdong Province.","PeriodicalId":8776,"journal":{"name":"Bing du xue bao = Chinese journal of virology","volume":"32 6","pages":"713-20"},"PeriodicalIF":0.0,"publicationDate":"2016-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36308993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Effect of PLSCR1 on the Antiviral Activity of IFN against HBV in HepG2 Cells]. PLSCR1对HepG2细胞中IFN抗病毒HBV活性的影响

Bing du xue bao = Chinese journal of virology Pub Date : 2016-11-01

Qingjun Li, Bo Zhang, Qiling Zhang, Xin Wang, Yujia Huo, Jing Yang

{"title":"[Effect of PLSCR1 on the Antiviral Activity of IFN against HBV in HepG2 Cells].","authors":"Qingjun Li, Bo Zhang, Qiling Zhang, Xin Wang, Yujia Huo, Jing Yang","doi":"","DOIUrl":"","url":null,"abstract":"To study the effect of interferon(IFN)against hepatitis B virus(HBV)by silencing phospholipid scramblase (PLSCR)1in HepG2 cells. siRNA specific for PLSCR1 was designed and transfected in HepG2 cells. The inhibitory effect of siRNA was determined using semi-quantitative polymerase chain reaction(PCR)and western blotting 48hpost-transfection.HepG2 cells treated with IFN were co-transfected with plasmids expressing HBV1.3and siRNA targeting PLSCR1.Total RNA of HepG2 cells was isolated and the mRNA level of PLSCR1 measured by reverse-transcription semi-quantitative PCR. The expression of HBsAg in culture supernatants was determined by enzyme-linked immunosorbent assay. Expression of PLSCR1 was inhibited by siRNA911 in HepG2cells.Compared with the control, the level of HBsAg decreased in the cell supernatants of cells transfected with HBV1.3plasmid or NC-siRNA + HBV1.3plasmid.Compared with cells not treated with IFN, the level of HBsAg did not change significantly in the supernatants of cells transfected with siRNA + HBV1.3plasmid and treated with IFN. Inhibition of PLSCR1 could decrease the antiviral activity of IFN against HBV. These data suggest that PLSCR1 has an important role in the inhibition of HBV replication due to IFN.","PeriodicalId":8776,"journal":{"name":"Bing du xue bao = Chinese journal of virology","volume":"32 6","pages":"747-51"},"PeriodicalIF":0.0,"publicationDate":"2016-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36308998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The Effect of Influenza Type B Inter-lineage Reassortment on the Growth Characteristics of Influenza]. 乙型流感病毒株间基因重组对流感病毒生长特性的影响

Bing du xue bao = Chinese journal of virology Pub Date : 2016-11-01

Yao Zhang, Jianfang Zhou, Xiyan Li, Jian Lu, Kun Qin, Yuelong Shu, Dayan Wang

{"title":"[The Effect of Influenza Type B Inter-lineage Reassortment on the Growth Characteristics of Influenza].","authors":"Yao Zhang, Jianfang Zhou, Xiyan Li, Jian Lu, Kun Qin, Yuelong Shu, Dayan Wang","doi":"","DOIUrl":"","url":null,"abstract":"This study aims to research the effect of inter-lineage reassortment of type B influenza virus on its biological characteristics. The representative strains isolated in 2013~2015were selected, which included wild type viruses and inter-lineage reassortment viruses. We tested the growth curve of each virus based on the value of TCID50 at different time point. And further detected the Km value of virus to analysis the activity of neuraminidase of each virus. The growth curve of viruses in 2013 and 2014reached peak 48 hours after infection, maintained at high level until 72 hours, then the virus titer declined gradually, however the virus isolated in 2015 reached peak 24 hours after infection. The reassortment strain B/Fujian Tongan/1565/2013 has the similar growth characteristics with the wild type virus on the same year. Meanwhile the growth curve of other inter-lineage viruses in 2014 and 2015is lower than that of wild type viruses. We determined neuraminidase kinetic constants of all viruses. The reassortment strain B/Fujian Tongan/1565/2013 has the strongest affinity of neuraminidase. The results of 2014~2015virus suggest that the stronger affinity of neuraminidase of virus the better growth characteristics of virus. But the virus in 2013 is lack of such relationship between the growth characteristics and activity of neuraminidase. The neuraminidase activity and growth characteristics of inter-lineage reassortments due to surface protein gene exchange is not consistent, suggesting internal virus protein might have affected the growth characteristics of the viruses2013-2015,and pending further study.","PeriodicalId":8776,"journal":{"name":"Bing du xue bao = Chinese journal of virology","volume":"32 6","pages":"768-72"},"PeriodicalIF":0.0,"publicationDate":"2016-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36309477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Preparation and Preliminary Application of a Virus Library for the Neutralizing Activity of a Monoclonal Antibody Against the Rabies Virus]. [狂犬病病毒单克隆抗体中和活性病毒文库的制备及初步应用]。

Bing du xue bao = Chinese journal of virology Pub Date : 2016-11-01

Pengcheng Yu, Xiaoyan Tao, Lihua Wang, Qing Tang, Shuqing Liu, Wuyang Zhu

引用次数: 0

[Canine Parvovirusin Diarrheal Dogs and Analyses of the Full-Length VP2 Gene of Dogs]. 犬细小病毒腹泻犬及其VP2全长基因分析[j]。

Bing du xue bao = Chinese journal of virology Pub Date : 2016-11-01

Xuefeng Cao, Guangneng Peng, Xiangming Huang, Wenbo Wang, Yinan Tian, Xiaoyang Xu, Shuangshuang Lei, Yanyi Yang, Chao Gong, Kuixing Yang, Bo Yuan, Zhihua Ren, Zhijun Zhong

引用次数: 0

[Advances in the Role of Stem Memory T Cells in HIV-1 Infection]. [干细胞记忆T细胞在HIV-1感染中的作用进展]。

Bing du xue bao = Chinese journal of virology Pub Date : 2016-11-01

Xiaofan Lu, Li Li, Huan Xia, Hao Wu, Bin Su, Tong Zhang

引用次数: 0