Biochemistry and molecular biology international最新文献_第2页

Inhibition of eukaryotic dna polymerase alpha by persimmon (Diospyros kaki) extract and related polyphenols. 柿子提取物及相关多酚对真核dna聚合酶α的抑制作用。

Biochemistry and molecular biology international Pub Date : 1999-05-01 DOI: 10.1080/15216549900201883

H Umekawa, Y Takada, Y Furuichi, T Takahashi, Y Achiwa, T Komiya, S Yoshida

引用次数: 5

Chemical modification studies on the glucose/mannose specific lectins from field and lablab beans. 大豆中葡萄糖/甘露糖特异性凝集素的化学改性研究。

Biochemistry and molecular biology international Pub Date : 1999-05-01 DOI: 10.1080/15216549900201913

S K Nadimpalli

{"title":"Chemical modification studies on the glucose/mannose specific lectins from field and lablab beans.","authors":"S K Nadimpalli","doi":"10.1080/15216549900201913","DOIUrl":"https://doi.org/10.1080/15216549900201913","url":null,"abstract":"Seeds of Dolichos lablab var. lignosus (field beans) and variety typicus (lablab beans) contain glucose/mannose specific lectins that have been affinity purified and well characterised (Siva Kumar N., and Rajagopal Rao, D., J.Biosci., 1986, 10, 95-109, (1) Rajasekhar et al., (Biochem.Archives. 1997, 13, 233-240) (2). Purified lectins are glycoproteins with a native molecular mass of 60 kDa and are made of two types of subunits (Gowda et al., 1994, J.Biol.Chem. 269, 18789-18793) (3). Chemical modifications of various groups in purified lectins (using group specific reagents) such as lysine (citraconic anhydride), carboxyl groups (water soluble carbodiimide) tyrosine (N-acetyl imidazole) and tryptophan (2-hydroxy 5-nitro benzylbromide) revealed that 14 out of 21 residues of lysines 7 out of 92 residues of carboxyl groups, 16 out of 24 tyrosine residues and 2 out of 10 tryptophan residues were modified. Lysine and carboxyl group modification led to 95% loss in haemaglutinating activity compared to control while tyrosine and tryptophan modifications led to complete loss of lectin activity. Arginine and histidine modifications led to only 50% loss in activity. The extent of modification and loss in activity was same when the lysine and carboxyl groups were modified in the presence and absence of the inhibitory sugar methyl alpha-D-glucopyranoside at 0.1 M concentration. However protection of modification and lectin activity was observed when the tyrosine and tryptophan residues were modified in the presence of the inhibitory sugar. Earlier CD studies carried out (1) and extensive chemical modification studies reported here substantiate the involvement of tyrosine and tryptophan residues in the sugar binding site of these lectins.","PeriodicalId":8770,"journal":{"name":"Biochemistry and molecular biology international","volume":"47 5","pages":"825-34"},"PeriodicalIF":0.0,"publicationDate":"1999-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15216549900201913","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21234013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Nucleoid proteins of pea chloroplasts: detection of a protein homologous to ribosomal protein. 豌豆叶绿体的核糖体蛋白：检测到一种与核糖体蛋白同源的蛋白。

Biochemistry and molecular biology international Pub Date : 1999-05-01 DOI: 10.1080/15216549900201843

Oleskina YuP, N P Yurina, T I Odintsova, T A Egorov, A Otto, B Wittmann-Liebold, M S Odintsova

引用次数: 9

C-terminal region of hTPO is important for secretion and expression in insect cells. hTPO的c端区对昆虫细胞的分泌和表达具有重要意义。

Biochemistry and molecular biology international Pub Date : 1999-05-01 DOI: 10.1080/15216549900201803

H K Ahn, J Y Chung, S K Park, S M Joo, S K Park, Y W Koh

引用次数: 4

Cloning, sequencing, expression and characterization of the manganese superoxide dismutase gene from Vibrio alginolyticus. 溶藻弧菌锰超氧化物歧化酶基因的克隆、测序、表达及特性分析。

Biochemistry and molecular biology international Pub Date : 1999-05-01 DOI: 10.1080/15216549900201893

Y C Shyu, C C Chiu, F P Lin

引用次数: 4

Categorizing reactivity of bacteriorhodopsin cysteine mutants crosslinking to 4-bromoretinal. 细菌视紫红质半胱氨酸突变体与4-溴视网膜交联的反应性分类。

Biochemistry and molecular biology international Pub Date : 1999-05-01 DOI: 10.1080/15216549900201863

N Datta-Gupta, R K Crouch, E S Hazard

引用次数: 0

Mitochondrial changes during the apoptotic process of HeLa cells exposed to cisplatin. 顺铂作用下HeLa细胞凋亡过程中线粒体的变化。

Biochemistry and molecular biology international Pub Date : 1999-05-01 DOI: 10.1080/15216549900201853

J Melendez-Zajgla, E Cruz, V Maldonado, A M Espinoza

引用次数: 30

Phenotypic selection: a successful strategy to fix major genes of hypertension. 表型选择:固定高血压主要基因的成功策略。

Biochemistry and molecular biology international Pub Date : 1999-05-01 DOI: 10.1080/15216549900201813

I Klöting, P Kovács, J van den Brandt

引用次数: 0

Kinetic properties of ATP-dependent phosphofructokinase from grapefruit juice sacs: effect of TCA cycle intermediates. 葡萄柚汁囊中atp依赖性磷酸果糖激酶的动力学性质:TCA循环中间体的影响。

Biochemistry and molecular biology international Pub Date : 1999-05-01 DOI: 10.1080/15216549900201833

E Van Praag, U Zehavi, R Goren

引用次数: 4

Induction of apoptosis by selenite and selenodiglutathione in HL-60 cells: correlation with cytotoxicity. 亚硒酸盐和硒二谷胱甘肽诱导HL-60细胞凋亡:与细胞毒性的相关性。

Biochemistry and molecular biology international Pub Date : 1999-05-01 DOI: 10.1080/15216549900201873

D Y Cho, U Jung, A S Chung

{"title":"Induction of apoptosis by selenite and selenodiglutathione in HL-60 cells: correlation with cytotoxicity.","authors":"D Y Cho, U Jung, A S Chung","doi":"10.1080/15216549900201873","DOIUrl":"https://doi.org/10.1080/15216549900201873","url":null,"abstract":"Effects of selenite and selenodiglutathione, an initial metabolite of selenite, on the induction of apoptosis and cytotoxicity were investigated in human promyelocytic leukemia HL-60 cells. Treatment of selenite or selenodiglutathione resulted in concentration-dependent cytotoxicity, measured by lactate dehydrogenase leakage assay, and by tetrazolium salt reduction assay. Selenodiglutathione has been shown to exert more cytotoxic effect than selenite in both assay systems. Time-course study of cellular selenium uptake suggests that the higher cytotoxicity of selenodiglutathione be largely due to faster and greater selenium uptake rate. Treatment with selenite or selenodiglutathione also induced apoptosis in a dose-dependent manner, as detected by enzyme-linked immunosorbent assay and by DNA fragmentation assay. The dose-response data of apoptosis induced by selenite or selenodiglutathione were similar to those of cytotoxicity, implicating a relationship between the induction of apoptosis and cytotoxicity. Zn, which is a well-known inhibitor of apoptosis, dose-dependently blocked not only the induction of apoptosis, but also the membrane damage induced by selenium, corroborating this hypothesis. It was noted that the inhibition of apoptosis by Zn exerted little protective effect on cytotoxicity at higher concentrations of selenium, compared with a perfect protective effect at low concentration of selenium. These results suggest that cytotoxicity induced by selenium may be partially correlated with apoptosis.","PeriodicalId":8770,"journal":{"name":"Biochemistry and molecular biology international","volume":"47 5","pages":"781-93"},"PeriodicalIF":0.0,"publicationDate":"1999-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15216549900201873","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21234135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 45