Biochemistry and molecular biology international最新文献_第3页

Anti-tumor polysaccharide from the mycelium of liquid-cultured Agaricus blazei mill. 液体培养姬松茸菌丝体的抗肿瘤多糖。

Biochemistry and molecular biology international Pub Date : 1999-04-01 DOI: 10.1080/15216549900201773

M Mizuno, K Minato, H Ito, M Kawade, H Terai, H Tsuchida

引用次数: 126

Protein kinase C isoforms in the epidermal tissues of normal and postburn human skin. 蛋白激酶C在正常和烧伤后人皮肤表皮组织中的异构体。

Biochemistry and molecular biology international Pub Date : 1999-04-01 DOI: 10.1080/15216549900201733

Y C Wang, Y S Hsieh, Y W Tang, J Y Liu

引用次数: 6

Lipoprotein alterations, abdominal fat distribution and breast cancer. 脂蛋白改变，腹部脂肪分布与乳腺癌。

Biochemistry and molecular biology international Pub Date : 1999-04-01 DOI: 10.1080/15216549900201743

L E Schreier, G A Berg, F M Basilio, G I Lopez, A E Etkin, R L Wikinski

引用次数: 39

Characterization of the human CR1 gene promoter. 人CR1基因启动子的表征。

Biochemistry and molecular biology international Pub Date : 1999-04-01 DOI: 10.1080/15216549900201713

J H Kim, S Lee, S Y Choe

引用次数: 13

Isolation and characterization of an arginine ester hydrolase from Bothrops jararacussu venom which induces contractions of the isolated rat uterus. 一种诱导离体大鼠子宫收缩的精氨酸酯水解酶的分离与鉴定。

Biochemistry and molecular biology international Pub Date : 1999-04-01 DOI: 10.1080/15216549900201763

S H Andrião-Escarso, A M Soares, V M Rodrigues, A C Mancin, M L Reis, G Ballejo, J R Giglio

{"title":"Isolation and characterization of an arginine ester hydrolase from Bothrops jararacussu venom which induces contractions of the isolated rat uterus.","authors":"S H Andrião-Escarso, A M Soares, V M Rodrigues, A C Mancin, M L Reis, G Ballejo, J R Giglio","doi":"10.1080/15216549900201763","DOIUrl":"https://doi.org/10.1080/15216549900201763","url":null,"abstract":"The isolation and partial characterization of a serine protease with arginine ester hydrolase activity from Bothrops jararacussu snake venom are described. The purification procedure consisted of a gel filtration of the crude venom on Sephadex G-75 followed by an ion-exchange chromatography of the active fraction on DEAE-cellulose and a rechromatography on Bio-Rex 70 resin. The esterase fraction (DI-III), M(r) = 25,000 by SDS-PAGE, showed proteolytic activity on fibrinogen and casein. After 2 hr incubation, the A alpha and B beta chains of fibrinogen were intensely hydrolysed, while the gamma chain kept apparently intact, even after 20 hr of incubation. In spite of that, DI-III did not clot fibrinogen. DI-III induced edema in the rat paw. Although unable to release bradykinin, it induced contractions of the isolated rat uterus. DI-III did not catalyse the hydrolysis of bradykinin. Its arginine ester hydrolase activity was completely inhibited by diisopropyl fluorophosphate after 1 hr incubation, but not by phenylmethylsulfonyl fluoride under the same conditions.","PeriodicalId":8770,"journal":{"name":"Biochemistry and molecular biology international","volume":"47 4","pages":"699-706"},"PeriodicalIF":0.0,"publicationDate":"1999-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15216549900201763","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21188206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Expression of the Na(+)-H+ exchanger isoform-1 and cyclooxygenases in human placentas: their implications in preeclampsia. 人胎盘中Na(+)-H+交换异构体-1和环加氧酶的表达:它们在子痫前期的意义。

Biochemistry and molecular biology international Pub Date : 1999-04-01 DOI: 10.1080/15216549900201783

I Khan, M al-Yatama, M Nandakumaran

{"title":"Expression of the Na(+)-H+ exchanger isoform-1 and cyclooxygenases in human placentas: their implications in preeclampsia.","authors":"I Khan, M al-Yatama, M Nandakumaran","doi":"10.1080/15216549900201783","DOIUrl":"https://doi.org/10.1080/15216549900201783","url":null,"abstract":"The sodium hydrogen exchanger isoform, NHE-1 plays an important role in electrolyte and water homeostasis. These functions are compromised in pregnancies complicated with preeclampsia. At present it is not known whether NHE-1 expression is altered during preeclampsia. In the present study the placental level of NHE-1 protein was measured using immunoblotting. Since prostaglandins regulate the secretory and absorptive functions, the levels of prostaglandin E-2 as well as the expression of cyclooxygenase-1 and -2 were also estimated. The amount of NHE-1 protein and cyclooxygenase-2 was reduced in preeclamptic placentas, whereas the level of cyclooxygenase-1 remained unaltered. In contrast, prostaglandin E-2 concentration was higher in preeclampsia. Suppression of NHE-1 might render the placenta with impaired uptake of water and electrolytes and therefore may be involved in the pathogenesis of preeclampsia. While prostaglandin E-2 may play a role in preeclampsia, these findings discount the induction of cyclooxygenase-genes for this increase.","PeriodicalId":8770,"journal":{"name":"Biochemistry and molecular biology international","volume":"47 4","pages":"715-22"},"PeriodicalIF":0.0,"publicationDate":"1999-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15216549900201783","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21188208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

An NMR study of the structural basis of the wide range of pharmacological functions of acetylsalicylic acid. 核磁共振研究了乙酰水杨酸广泛的药理功能的结构基础。

Biochemistry and molecular biology international Pub Date : 1999-04-01 DOI: 10.1080/15216549900201723

J Li, H Huang, M Zhou, S Ning, X Jiang, Y Peng, K Zhao

引用次数: 4

Polymerization of actin induced by a molar excess of ATP in a low salt buffer. 在低盐缓冲液中，由摩尔过量的ATP引起的肌动蛋白聚合。

Biochemistry and molecular biology international Pub Date : 1999-04-01 DOI: 10.1080/15216549900201753

T Ikkai, H Kondo

引用次数: 1

The carboxyl-terminal fragment of osteopontin suppresses arginine-glycine-asparatic acid-dependent cell adhesion. 骨桥蛋白的羧基末端片段抑制精氨酸-甘氨酸-天冬氨酸依赖的细胞粘附。

Biochemistry and molecular biology international Pub Date : 1998-12-01 DOI: 10.1080/15216549800204632

K Takahashi, F Takahashi, K K Tanabe, H Takahashi, Y Fukuchi

{"title":"The carboxyl-terminal fragment of osteopontin suppresses arginine-glycine-asparatic acid-dependent cell adhesion.","authors":"K Takahashi, F Takahashi, K K Tanabe, H Takahashi, Y Fukuchi","doi":"10.1080/15216549800204632","DOIUrl":"https://doi.org/10.1080/15216549800204632","url":null,"abstract":"Osteopontin (OPN) is a secreted glycoprotein implicated in cell adhesion. It contains the arginine-glycine-asparatic acid (RGD) cell adhesive domain and the thrombin cleavage sequence. Although thrombin cleavage of OPN has been shown to be of physiological importance, the function of C-terminal OPN fragment cleaved by thrombin remains unknown. To determine its role, we performed cell adhesion assays using glutathione S-transferase-OPN fusion protein fragments and full-length OPN fusion protein. The N-terminal fragment containing RGD motif promoted enhanced adhesion of mouse and human fibroblasts by 2.9 and 2.8 folds in comparison with full-length OPN, respectively. The enhanced adhesion of both cells mediated by N-terminal fragment was significantly suppressed by addition of C-terminal fragment lacking RGD motif that has less cell adhesive property than full-length OPN. These results suggest that the C-terminal domain may play a pivotal role in regulating OPN functions by suppressing the RGD-dependent cell adhesion.","PeriodicalId":8770,"journal":{"name":"Biochemistry and molecular biology international","volume":"46 6","pages":"1081-92"},"PeriodicalIF":0.0,"publicationDate":"1998-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15216549800204632","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20798557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26

Suppression of constitutive and inducible cytochrome P450 gene expression by alpha-hederin in mice. α -hederin对小鼠组成型和诱导型细胞色素P450基因表达的抑制作用。

Biochemistry and molecular biology international Pub Date : 1998-12-01 DOI: 10.1080/15216549800204572

H G Jeong

{"title":"Suppression of constitutive and inducible cytochrome P450 gene expression by alpha-hederin in mice.","authors":"H G Jeong","doi":"10.1080/15216549800204572","DOIUrl":"https://doi.org/10.1080/15216549800204572","url":null,"abstract":"The effects of alpha-Hederin, a triterpenoid saponin which exists in some oriental herbs, on the expression of liver cytochrome P450s were examined in mice. The administration of alpha-Hederin to mice significantly decreased the hepatic content of P450 and the activities of microsomal ethoxyresorufin O-deethylase, methoxyresorufin O-demethylase, and aniline hydroxylase, representative activities of cytochrome-P4501A1, -P4501A2, and -P4502E1, respectively, in a dose- and time-dependent manner. However, pentoxyresorufin O-dealkylase, a representative activity of cytochrome P4502B1/2, was decreased to a lesser extent. alpha-Hederin also decreased inducible monooxygenase activities in the same manner. Suppressions of P450 isozyme expression occurred in alpha-Hederin treated hepatic microsomes, as determined by immunoblot analysis in a manner consistent with that of the enzyme activity levels. Levels of mRNA of P4501A1/2 and P4502B1/2 were also decreased by alpha-Hederin as shown by Northern blot analysis. In contrast, the level of P4502E1 mRNA in the liver of alpha-Hederin treated mice was unchanged. These results suggest that alpha-Hederin may act as a more specific suppressor for P4501A and P4502E1 than P4502B and that the suppression involves decreases in mRNA levels except in the case of P4502E1.","PeriodicalId":8770,"journal":{"name":"Biochemistry and molecular biology international","volume":"46 5","pages":"1019-26"},"PeriodicalIF":0.0,"publicationDate":"1998-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/15216549800204572","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20769742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9