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Comparison of enzyme-linked immunosorbent assay, electron microscopy, and reversed passive haemagglutination for detection of human rotavirus in stool specimens. 酶联免疫吸附法、电子显微镜和反向被动血凝检测粪便标本中人轮状病毒的比较
Biken journal Pub Date : 1983-06-01
H Tsuchie, K Shimase, I Tamura, O Kurimura, E Kaneto, T Katsumoto, M Ito, T Kurimura
{"title":"Comparison of enzyme-linked immunosorbent assay, electron microscopy, and reversed passive haemagglutination for detection of human rotavirus in stool specimens.","authors":"H Tsuchie,&nbsp;K Shimase,&nbsp;I Tamura,&nbsp;O Kurimura,&nbsp;E Kaneto,&nbsp;T Katsumoto,&nbsp;M Ito,&nbsp;T Kurimura","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>An enzyme-linked immunosorbent assay (ELISA) using microplates as solid phase, rabbit antiserum against human rotavirus Wa strain as catching antibody, and the same reagent labeled with beta-D-galactosidase as conjugate, has been developed for detection of human rotavirus antigen(s) in stool specimens from patients with acute gastroenteritis. The limit of detection of purified human rotavirus by ELISA was 15.6 ng/ml (1.56 ng/well) of viral protein. The sensitivities of ELISA, electron microscopy, and the reversed passive haemagglutination method (ROTA-CELL) were compared. ELISA was more sensitive than electron microscopy and the reversed passive haemagglutination method. The ELISA blocking assay was useful for detection of an antibody response to human rotavirus in paired sera from children in two institutions during outbreaks of rotavirus gastroenteritis.</p>","PeriodicalId":8767,"journal":{"name":"Biken journal","volume":"26 2","pages":"87-92"},"PeriodicalIF":0.0,"publicationDate":"1983-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17380314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Interferon-alpha in serum and carcinomatous pleural effusion after repeated intrapleural injections of antitumor agents. 反复胸腔内注射抗肿瘤药物后血清和癌性胸腔积液中的干扰素- α。
Biken journal Pub Date : 1983-06-01
E Kaneto, H Sasaki, I Tamura, O Kurimura, T Kurimura
{"title":"Interferon-alpha in serum and carcinomatous pleural effusion after repeated intrapleural injections of antitumor agents.","authors":"E Kaneto,&nbsp;H Sasaki,&nbsp;I Tamura,&nbsp;O Kurimura,&nbsp;T Kurimura","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Pleural effusions and sera of two patients with lung cancer were tested after intrapleural injection of OK-432 as an anticancer drug for IFN-alpha activity by biological assay and for IFN-alpha as an antigen by radioimmunoassay. The titers by radioimmunoassay were fairly consistent with those by biological assay, but were usually higher. In Case 1, IFN-alpha was observed fairly early after administration of OK-432 and only in pleural effusions. In Case 2, induction of IFN-alpha at low level was observed late after the first administration of OK-432 both in the pleural effusion and serum and was detected only by radioimmunoassay.</p>","PeriodicalId":8767,"journal":{"name":"Biken journal","volume":"26 2","pages":"99-102"},"PeriodicalIF":0.0,"publicationDate":"1983-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17380315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Replication of herpes simplex virus in two cell systems derived from rhesus monkeys. 单纯疱疹病毒在源自恒河猴的两个细胞系统中的复制。
Biken journal Pub Date : 1983-03-01
M Yamada
{"title":"Replication of herpes simplex virus in two cell systems derived from rhesus monkeys.","authors":"M Yamada","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The replication of herpes simplex virus (HSV) in two cell systems derived from rhesus monkeys (LLC-MK2 and DBS-FRhL-2) was studied. In LLC-MK2, the growth of HSV-1 was abortive or extremely limited regardless of the multiplicity of infection, while that of HSV-2 was productive only on infection at high multiplicities. DBS-FRhL-2 cells supported growth of both types of HSV, although growth was highly dependent on the age of monolayers and the infectious dose of virus inocula. Plaques were produced in DBS-FRhL-2 cell monolayers inoculated with HSV-2 but not with HSV-1, although the efficiency of their formation in the former system was much less than in a system of FL and HSV-2. On the other hand, plaques were not produced in LLC-MK2 cell monolayers by either type of HSV. The growth of adapted variants of HSV-1 was also studied. In contrast to the parental strain, these variants replicated well in LLC-MK2 even at a low multiplicity of infection and produced clear plaques in the monolayers. Furthermore, persistent infections of HSV-2 were established in DBS-FRhL-2 cell monolayers under routine culture conditions.</p>","PeriodicalId":8767,"journal":{"name":"Biken journal","volume":"26 1","pages":"35-47"},"PeriodicalIF":0.0,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17373833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Studies on the surface component of Trypanosoma cruzi amastigotes: determination of the specific antigen and characterization by enzyme treatment and electronmicroscopy. 克氏锥虫无尾虫表面成分的研究:特异性抗原的测定及酶处理和电镜表征。
Biken journal Pub Date : 1983-03-01
H Kanbara, K Kototani, T Nakabayashi
{"title":"Studies on the surface component of Trypanosoma cruzi amastigotes: determination of the specific antigen and characterization by enzyme treatment and electronmicroscopy.","authors":"H Kanbara,&nbsp;K Kototani,&nbsp;T Nakabayashi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A specific antigen to amastigotes of Trypanosoma cruzi was found by immunodiffusion. It was one of the predominant antigens against anti-amastigote serum and also against infected rabbit serum. This specific antigen was mainly eluted in the first peak from a Sephadex G-200 column and moved slowly on immunoelectrophoresis. It was shown to be present on the surface of amastigotes using antiserum to the specific precipitin line between the first fraction of amastigote antigen from the Sephadex G-200 column and the anti-amastigote serum. The surface component of amastigotes was found to be rich in carbohydrate by histochemical electronmicroscopy. The component was also shown to be susceptible to pronase and hyaluronidase, but resistant to trypsin, chondroitinase ABC and neuraminidase, by investigating the sensitivity to the lytic effect of fresh serum from unimmunized rabbits after enzyme treatment. The relation between the specific antigen and the surface component was discussed.</p>","PeriodicalId":8767,"journal":{"name":"Biken journal","volume":"26 1","pages":"49-55"},"PeriodicalIF":0.0,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17474352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Purification and molecular anatomy of the varicella-zoster virion. 水痘-带状疱疹病毒粒子的纯化及分子解剖。
Biken journal Pub Date : 1983-03-01
C Grose, W E Friedrichs, G C Smith
{"title":"Purification and molecular anatomy of the varicella-zoster virion.","authors":"C Grose,&nbsp;W E Friedrichs,&nbsp;G C Smith","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Varicella-zoster virus (VZV) infected cell cultures were harvested and sonically disrupted when cytopathic effect was advanced. Infectious cell-free virus in the sonicates, as well as that in the culture medium, was further concentrated by precipitation with 8% (w/v) polyethylene glycol in the presence of high salinity (0.5 M). The virus-enriched pellet was layered onto 15-45% linear metrizamide gradients and sedimented for 18 h at 70,000 g. Of the three visible bands (designated upper, middle and lower), the middle band at a buoyant density of 1.156-7 g/cm3 was enriched for enveloped virions. Electron microscopic enumeration of particles demonstrated a total of 10.04 log10 enveloped particles and 8.26 log10 unenveloped particles from middle bands representing the yield from a 150 cm2 VZV-infected monolayer. Fractionation of radiolabeled virion preparations by SDS-PAGE revealed 30 polypeptides between 30 and 200 kilodaltons (K) with a total mol wt of 2,240,000. Prominent structural polypeptides included the major capsid protein (155K) and three glycoproteins--62K, 98K and 118K. Certain polypeptides better labeled by [14C] amino acids than by [35S] methionine included a higher mol wt polypeptide (174K) and 45K protein comigrating with actin. Immune precipitation of a Nonidet-extracted virion fraction again demonstrated the three major glycoproteins, as well as the 155K and 45K polypeptides. Comparison of structural polypeptides with the 16 constituents of the VZV-specific immunoprecipitation profile identified at least one polypeptide (145K) which was not represented in the virion and assumed, therefore, to be nonstructural.</p>","PeriodicalId":8767,"journal":{"name":"Biken journal","volume":"26 1","pages":"1-15"},"PeriodicalIF":0.0,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17373830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolation of drug resistant mutants of varicella-zoster virus: cross resistance of acyclovir resistant mutants with phosphonoacetic acid and bromodeoxyuridine. 水痘-带状疱疹病毒耐药突变体的分离:无环鸟苷耐药突变体与膦乙酸和溴脱氧尿苷的交叉耐药。
Biken journal Pub Date : 1983-03-01
K Shiraki, T Ogino, K Yamanishi, M Takahashi
{"title":"Isolation of drug resistant mutants of varicella-zoster virus: cross resistance of acyclovir resistant mutants with phosphonoacetic acid and bromodeoxyuridine.","authors":"K Shiraki,&nbsp;T Ogino,&nbsp;K Yamanishi,&nbsp;M Takahashi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Mutants of Varicella-Zoster Virus (VZV) which are resistant to phosphonoacetic acid (PAA), bromodeoxyuridine (BuDR), and acyclovir (ACV) were obtained by serial passages of VZV with increasing concentrations of these drugs. A PAA-resistant mutant and a BuDR-resistant mutant were found also to be resistant to ACV. Five of 8 ACV-resistant mutants acquired resistance to PAA, but none acquired resistance to BuDR. The BuDR-resistant mutant did not induce viral thymidine kinase (TK) activity, but all the ACV-resistant mutants selected in ACV showed viral TK activity which was suppressed with anti-VZV serum and had almost the same electrophoretic mobility as that of the parent strain on polyacrylamide gel electrophoresis in non-denaturing conditions. However, in competitive TK assay with ACV, 2 of 8 ACV-resistant mutants showed no change of phosphorylation of radioactive thymidine, while the other 6 showed decreased phosphorylation of radioactive thymidine. It was suggested that TK induced by the former 2 ACV-resistant mutants had lost affinity to ACV, and so the mutants could grow in the presence of ACV. Thus of the 8 ACV-resistant mutants selected in ACV, 2 were sensitive to PAA with altered TK activity, 5 were resistant to PAA with unaltered TK activity, and 1 was sensitive to PAA with unaltered TK activity, and may have altered DNA polymerase activity to ACV, retaining sensitivity to PAA. These results suggest that resistance of VZV to ACV results from alterations in the virus-specified TK or DNA polymerase, as demonstrated in HSV resistant to ACV.</p>","PeriodicalId":8767,"journal":{"name":"Biken journal","volume":"26 1","pages":"17-23"},"PeriodicalIF":0.0,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17373831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Neutralizing antibody responses to Japanese encephalitis vaccine in children. 儿童乙型脑炎疫苗中和抗体反应。
Biken journal Pub Date : 1983-03-01
R F Juang, Y Okuno, T Fukunaga, M Tadano, K Fukai, K Baba, N Tsuda, A Yamada, H Yabuuchi
{"title":"Neutralizing antibody responses to Japanese encephalitis vaccine in children.","authors":"R F Juang,&nbsp;Y Okuno,&nbsp;T Fukunaga,&nbsp;M Tadano,&nbsp;K Fukai,&nbsp;K Baba,&nbsp;N Tsuda,&nbsp;A Yamada,&nbsp;H Yabuuchi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Two shots of the current Japanese encephalitis (JE) vaccine were given to children and their immune responses to the Nakayama strain (the vaccine strain) and two wild strains (JaGAr-01 and E-50) of JE virus were examined by neutralizing (N) antibody titrations. Seventy vaccinees had no N antibody to JE virus before the first vaccination and were bled one month after the second vaccination. The N antibody responses to the JaGAr-01 and E-50 strains were found to be similar and to be less than that to the Nakayama strain after the second vaccination: the geometric mean titers (GMT) of N antibodies to the JaGAr-01 and E-50 strains (as logarithms) were 1.87 and 1.75, respectively, while the GMT to the Nakayama strain was 2.89. The seroconversion rates to the Nakayama, JaGAr-01 and E-50 strains were 70/70 (100%), 69/70 (99%) and 68/70 (97%), respectively, after the second vaccination. Twenty-seven of the 70 vacciness were also bled before the second vaccination. Most of them showed a considerably high N antibody response against the Nakayama strain and only one vaccinee failed to show seroconversion after the first vaccination. However, the antibody response to the E-50 strain appeared to be rather low and 9 of 25 vaccinees did not show any seroconversion. Similarly 3 of 25 failed to show any seroconversion against the JaGAr-01 strain. These results indicate that at the initial immunization two shots, at least, of the current JE vaccine are necessary to stimulate effective immune responses to wild strains of JE virus.</p>","PeriodicalId":8767,"journal":{"name":"Biken journal","volume":"26 1","pages":"25-34"},"PeriodicalIF":0.0,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17681185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative studies on trypomastigotes of Trypanosoma cruzi from infected mouse blood and infected fibroblast cell (L-cell) culture. 克氏锥虫感染小鼠血液与感染成纤维细胞(l细胞)培养的锥虫乳突体的比较研究。
Biken journal Pub Date : 1983-03-01
H Kanbara, T Nakabayashi
{"title":"Comparative studies on trypomastigotes of Trypanosoma cruzi from infected mouse blood and infected fibroblast cell (L-cell) culture.","authors":"H Kanbara,&nbsp;T Nakabayashi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The resistance to phagocytosis by macrophages and the penetration into fibroblast cells of blood form trypomastigotes of Trypanosoma cruzi were compared with those of trypomastigotes grown in fibroblast cell culture. On incubation for 24 h, blood form trypomastigotes were almost completely resistant to phagocytosis, but about 40% of the trypomastigotes from cell culture were phagocytized. On longer incubation, the resistance of both forms of trypomastigotes decreased gradually. The penetrating ability of blood form trypomastigotes was much lower than that of trypomastigotes from cell culture. Infection of mice with blood form trypomastigotes resulted in less proliferation of parasites in the liver and spleen than that with trypomastigotes from cell culture. From these results, the existence of two functionally different forms of trypomastigotes in infected mice and in infected fibroblast cell culture, respectively, is discussed.</p>","PeriodicalId":8767,"journal":{"name":"Biken journal","volume":"26 1","pages":"57-62"},"PeriodicalIF":0.0,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17474353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Improved method for separation on CM-cellulose of the trypomastigote form of Trypanosoma cruzi from forms grown in fibroblast cell cultures. 克氏锥虫锥体型与成纤维细胞培养的cm -纤维素分离方法的改进。
Biken journal Pub Date : 1983-03-01
H Kanbara, T Nakabayashi
{"title":"Improved method for separation on CM-cellulose of the trypomastigote form of Trypanosoma cruzi from forms grown in fibroblast cell cultures.","authors":"H Kanbara,&nbsp;T Nakabayashi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Trypomastigotes grown in fibroblast cell (L-cell) culture were more effectively and more rapidly separated from other cells on a CM-cellulose column with culture medium (MEM with 10% calf serum) for elution instead of phosphate-saline-glucose buffer (PSG). This effective separation was shown to be due to the presence of serum. Trypomastigotes weakly adhered to CM-cellulose resin by the tip of the body (mainly the flagellar tip) when they were suspended with CM-cellulose resin in PSG. Serum seemed to disturb the adhesion of trypomastigotes to the resin, but not the adhesion of amastigotes and fibroblast cells. Therefore, only trypomastigotes were rapidly eluted from a CM-cellulose column in the presence of serum.</p>","PeriodicalId":8767,"journal":{"name":"Biken journal","volume":"26 1","pages":"63-5"},"PeriodicalIF":0.0,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17414165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
DNA binding proteins induced by varicella-zoster virus in human cells. 水痘-带状疱疹病毒在人细胞中诱导的DNA结合蛋白。
Biken journal Pub Date : 1982-12-01
K Shiraki, T Yamamoto, K Yamanishi, M Takahashi
{"title":"DNA binding proteins induced by varicella-zoster virus in human cells.","authors":"K Shiraki,&nbsp;T Yamamoto,&nbsp;K Yamanishi,&nbsp;M Takahashi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>DNA-binding proteins induced by varicella-zoster virus (VZV) were detected by native-and denatured-DNA affinity chromatography. Eleven DNA-binding proteins specific to the infected cells were separated by SDS polyacrylamide gel electrophoresis (PAGE); their molecular weights ranged from 180 X 10(3) to 22 X 10(3). When they were applied to an affinity column coupled with VZV-antibody, a polypeptide with a molecular weight of 34 X 10(3) was predominant on subsequent SDS-PAGE.</p>","PeriodicalId":8767,"journal":{"name":"Biken journal","volume":"25 4","pages":"185-9"},"PeriodicalIF":0.0,"publicationDate":"1982-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17368502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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