{"title":"水痘-带状疱疹病毒在人细胞中诱导的DNA结合蛋白。","authors":"K Shiraki, T Yamamoto, K Yamanishi, M Takahashi","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>DNA-binding proteins induced by varicella-zoster virus (VZV) were detected by native-and denatured-DNA affinity chromatography. Eleven DNA-binding proteins specific to the infected cells were separated by SDS polyacrylamide gel electrophoresis (PAGE); their molecular weights ranged from 180 X 10(3) to 22 X 10(3). When they were applied to an affinity column coupled with VZV-antibody, a polypeptide with a molecular weight of 34 X 10(3) was predominant on subsequent SDS-PAGE.</p>","PeriodicalId":8767,"journal":{"name":"Biken journal","volume":"25 4","pages":"185-9"},"PeriodicalIF":0.0000,"publicationDate":"1982-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"DNA binding proteins induced by varicella-zoster virus in human cells.\",\"authors\":\"K Shiraki, T Yamamoto, K Yamanishi, M Takahashi\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>DNA-binding proteins induced by varicella-zoster virus (VZV) were detected by native-and denatured-DNA affinity chromatography. Eleven DNA-binding proteins specific to the infected cells were separated by SDS polyacrylamide gel electrophoresis (PAGE); their molecular weights ranged from 180 X 10(3) to 22 X 10(3). When they were applied to an affinity column coupled with VZV-antibody, a polypeptide with a molecular weight of 34 X 10(3) was predominant on subsequent SDS-PAGE.</p>\",\"PeriodicalId\":8767,\"journal\":{\"name\":\"Biken journal\",\"volume\":\"25 4\",\"pages\":\"185-9\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1982-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biken journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biken journal","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
采用天然和变性dna亲和层析法检测水痘-带状疱疹病毒(VZV)诱导的dna结合蛋白。用SDS -聚丙烯酰胺凝胶电泳(PAGE)分离感染细胞特异性的11个dna结合蛋白;分子量在180 × 10(3) ~ 22 × 10(3)之间。当将它们应用于与vzv抗体偶联的亲和柱时，在随后的SDS-PAGE上，分子量为34 X 10(3)的多肽占优势。
DNA binding proteins induced by varicella-zoster virus in human cells.
DNA-binding proteins induced by varicella-zoster virus (VZV) were detected by native-and denatured-DNA affinity chromatography. Eleven DNA-binding proteins specific to the infected cells were separated by SDS polyacrylamide gel electrophoresis (PAGE); their molecular weights ranged from 180 X 10(3) to 22 X 10(3). When they were applied to an affinity column coupled with VZV-antibody, a polypeptide with a molecular weight of 34 X 10(3) was predominant on subsequent SDS-PAGE.
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